Browse > Article
http://dx.doi.org/10.5010/JPB.2003.30.2.151

Factors Affecting Plant Regeneration in the Culture of Different Explants of Stevia(Stebia rebaudiana Bertoni)  

Park, Gyu-Hwan (Department of Plant Resources, College of Life and Resources, Sangju National University)
Kim, Kyung-Min (Institute of Genetic Engineering, Kyungpook National University)
Publication Information
Journal of Plant Biotechnology / v.30, no.2, 2003 , pp. 151-154 More about this Journal
Abstract
This study was conducted to determine the optimal concentrations of plant hormones (2,4-D, picloram, dicamba, NAA, kinetin) and the suitable explants among seeds, hypocotyl, and cotyledons on calls formation and plant regeneration of stevia(Stevia rebaudiana Bertoni). The frequency of cellus formation was higher in the young leaf-explants then the older ones, and in the seeds then the hypocotyls and cotyledons on MS medium with 1mg/L 2,4-D. After transfer of seed-derived stevia callus producing embryogenic callus on plant-regeneration medium, the frequency of plant regeneration from callus was 23.8% in MS medium with 1mg/L NAA and 3mg/L kinetin.
Keywords
Plant regeneration; seed; stevia;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bakal AI, Nabors LO (1990) Stevioside. In: Nabors LO, Gelardi RC (eds.), Alternative sweetners, Ed2. Marcel Dekker, New York, pp 295
2 Brown C, Brooks FJ, Pearson D, Mathias RJ (1989) Control of embryogenesis and organogenesis In immature wheat embryo callus using increased medium osmolarity and abscisic acid. J Plant Physiol 133: 727-733   DOI
3 Chae YA, Yu CY (1984) Plant regeneration from leaf explants in Stevia rebaudiana Bertoni. Korean J Plant Tiss Cult 11: 55-5
4 Thomas MR, Scott KJ (1985) Plant regeneration by somatic embryogenesis from callus initiated from immature embryos and immature inflorescences of Hordeum vulgare. J Plant Physlol 121: 159-169   DOI
5 Vasil V, Vasil lK (1984) Induction and maintenance of embryogenic callus cultures of Gramineae. In: Vasil lK (ed), Cell Culture and Senate Cell Genetcs of Rants. Vol 1, Academic Press, Orlando, Florida. pp 36-42
6 Yu CY, Chae YA (1984) In vitro propagation of Stevia rebaudiana Bertoni. Korean J Crop Sci 29: 102-107
7 Pareddy DR, Petolino JF (1990) Somatic embryogenesis and plant regeneration from immature inflorescences of several elite inbreds of maize. Plant Sci 67: 211-219   DOI   ScienceOn
8 Chen TH, Lam L, Glen SC (1985) Somatic embryogenesis and plant regeneration from cultured young inflorescences of Oryza sativaL (rice). Plant Cell Tiss Org Cult 4: 51-54   DOI
9 Franta R, Beck B (1986) Alternatives to cane and beet sugars. Food Technol 40: 116
10 Murashige T, Skoog F (1962) A recised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473-497   DOI
11 Park DC, Lee YH (1997) Production, transglycosylation and application of stevioside. Stevioside. Food Industry and Nutrition 2: 31-44
12 Swedlund B, Vasil lK (1985) Cytogenetic characterization of embryogenic callus and regenerated plants of Pennisetum americanum (L) K. Schum. Theor Appl Genet 69: 575-581   DOI   ScienceOn