Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Factors Affecting Plant Regeneration in the Culture of Different Explants of Stevia(Stebia rebaudiana Bertoni)

Stevia 절편체 종류의 배양에 따른 식물체 재분화

  • Park, Gyu-Hwan (Department of Plant Resources, College of Life and Resources, Sangju National University) ;
  • Kim, Kyung-Min (Institute of Genetic Engineering, Kyungpook National University)
  • 박규환 (상주대학교 생명자원과학대학 식물자원학과) ;
  • 김경민 (경북대학교 유전공학연구소)
  • Published : 2003.06.01
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Abstract

This study was conducted to determine the optimal concentrations of plant hormones (2,4-D, picloram, dicamba, NAA, kinetin) and the suitable explants among seeds, hypocotyl, and cotyledons on calls formation and plant regeneration of stevia(Stevia rebaudiana Bertoni). The frequency of cellus formation was higher in the young leaf-explants then the older ones, and in the seeds then the hypocotyls and cotyledons on MS medium with 1mg/L 2,4-D. After transfer of seed-derived stevia callus producing embryogenic callus on plant-regeneration medium, the frequency of plant regeneration from callus was 23.8% in MS medium with 1mg/L NAA and 3mg/L kinetin.

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References

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  1. Changes of Antioxidant Enzymes in Stevia Plants under Clinorotation, Shaking, and Low Temperature Stresses vol.24, pp.4, 2011, https://doi.org/10.7732/kjpr.2011.24.4.343