• Journal of Food Hygiene and Safety
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• v.7 no.1
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• pp.29-36
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• 1992

This study primarily attempted to establish the method for the determination of the adulteration in the sesame oil. First of all, extensive experiment was conducted to determine the composition of genuine sesame oil prepared from Korean, Japanese, Taiwanese and Chinese sesame seed. Sesamin and sterols in unsaponfiable matter were examined along with fatty acid in saponifiable fraction by Gc. There was no significant difference in the composition of sesamin and sterols in sesame oils prepared from Korean and foreign seeds. The ranges of sesamin and ${\beta}-sitosterol$ against campesterol were 3.32~5.46 and 2.39~2.99 respectively in all samples. Similiar composition of fatty acids was showed in all pure sesame oils, in which the contents were 8.37~lO.09% palmitic acid, 4.61~5.50% stearic acid, 35.24~39.97% oleic acid, 43.04~49.76% linoleic acid, O.21~O.31% linolenic acid and 0.40~O.69% arachidic acid. Among the commercial sesame oils sold in Markets, three sesame oils from Japan revealed low sesamin, high linoleic acid and linolenic acid, and low oleic acid and stearic acid, suggesting the adulteration with soybean oil.

• Korean Journal of Food Science and Technology
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• v.21 no.1
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• pp.17-24
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• 1989

Effects of cooking and drying methods on the lipids content and neutral lipid component of shrimp, Metapenaeus joyneri, were investigated. Total lipid content of the fresh shrimp was 6.0%(dry basis), which was not changed significantly depending on the cooking and drying methods. Lipid of the fresh shrimp was composed of 36.8% of neutral lipids, 21.5% of glycolipids, and 41.7% of phospholipids. Freeze dried shrimp was substantially higher in neutral lipid contents but lower in glycolipid and phospholipid contents than hot air dried one. Main components of the neutral lipids were triglycerides, free sterols, free fatty acids and esterified sterols. Freeze dried shrimp contained higher triglycerides contents than hot air dried shrimp, whereas free fatty acids content in hot air dried shrimp was greater than that of freeze dried one due to the decomposition of triglycerides to free fatty acids during hot air drying. Major fatty acid composition of the total lipid were palmitoleic acid, eicosapentaenoic acid and docosahexaenoic acid, and that of neutral lipid were palmitic acid, palmitic acid, oleic acid, eicosapentaenoic acid and docosahexaenoic acid in fresh shrimp.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.16 no.3
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• pp.255-259
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• 1983

This work was done in order to clarify the chemical characteristics, composition, fatty acid components and sterol components of the lipids from Urechis unicintus. The results obtained are summarized as follows ; 1. The lipid content, iodine value and unsaponifiable matter of the total lipids are 1.89, 111, and $14.3\%$, respectively. 2. The main components of the total lipids are phospholipids $39.8\%$, free sterol $27.7\%$ and triglyceride $21.4\%$, and two unidentified fractions are detected. 3. The main fatty acids of total lipids are $C_{20:4}(19.4\%),\;C_{16:0}(13.7\%),\;C_{20:1}(11.3\%)\;and\;C_{18:1}(10.4\%)$ 4. Sterols found are Cholesterol($57.6\%$), Brassicasterol(?)($20.3\%$), 24-methylenecholesterol ($17.7\%$),22-dehydrocholesterol($3.0\%$)and 22.trans-24-norcholesta-5,22-dien-$3{\beta}$-ol(?)$1.4\%$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.6
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• pp.770-775
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• 2014

The green microalga of the genus Nannochloropsis (class Eustigmatophyceae) is a leading candidate for biofuel production due to its ability to accumulate high oil content (28.7% of cellular ash-free dry weight). We investigated the anti-inflammatory and anticancer activities of sterol-rich fraction from nannochloropsis oculata n-hexane (NOH) extract after saponification of the microalga. Among the fractions with n-hexane, chloroform and ethyl acetate, the n-hexane fraction showed the highest anti-inflammatory activity in LPS-stimulated RAW 264.7 macrophage as well as anticancer activity against human leukemia HL-60 cells without the cytotoxity. And the sterol-rich fraction was obtained from the n-hexane fraction by open silica column under the gradient solvent condition with 100% hexane (1L), hexane : ethyl acetate (20 : 1, 10 : 1, 5 : 1, 1 : 1, v/v). Among the four fractions (NOH-1~4), especially NOH-1 contained the highest content of sterols. NOH1 showed the highest HL-60 (about 85%) and NO inhibitory activities at the concentration of $100{\mu}g/mL$. These results demonstrated that the sterol-rich fraction from N. oculata might be a useful candidate as anti-inflammatory and anticancer agents for anti-inflammatory and anticancer activity.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.3
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• pp.195-202
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• 1976

The present investigation was performed to find the lipid composition of the total lipids, the fatty acid components of the neutral lipids and the phospholipids, and the composition of sterols, from Spisula sachalinensis. The results obtained are as follows ; 1) The main components of the total lipids are phospholipids$(43.1\%)$, triglyceride$(36.2\%) $, and sterol $(10.3\%)$. 2) The phospholipids are mainly composed of phosphatidyl choline, phosphatidyl ethanols, mine, phosphatidal ethanolamine and phosphatidyl serine. 3) The main fatty acids of the neutral lipids, the ethanolamine phospholipids and choline phospholpids, are C20:5, C16:1, C16:0, C20:5, C18:0, C22:6 and C16:0, C20:5, C22:6, respectively. Oleic acid content of all fractions is very small compared with one of gastropoda lipids and fish oil. 4) Most of plasmalogen are present in the ethanolamine phospholipids and only trace of plasmalogen in the choline phospholipids. 5) Sterols to be found are 22-trans-norcholesta-5, 22-diene-$3\beta$-ol, 22-dehydrocholesterol, cholesterol, brassicasterol, 24-methylenecho-lesterol and $\beta-sitosterol$.

• Fisheries and Aquatic Sciences
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• v.15 no.3
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• pp.241-250
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• 2012

Polycyclic aromatic hydrocarbons (PAHs), butyltins (BTs), nonylphenol (NP), and fecal sterols concentrations in sediments were investigated from Gunsan coast of Korea to evaluate organic pollution from anthropogenic activities. Sediment toxicity was also examined by bacterial bioluminescence toxicity test (Vibrio fischeri). The concentrations of 16 PAHs in sediments ranged from 67.9 to 425 ng/g dry wt; BTs ranged from 2.79 to 14.1 ng Sn/g dry wt; NP ranged from 20.7 to 2171 ng/g dry wt; and coprostanol, a fecal sterol, ranged from 7.60 to 245 ng/g dry wt. Effective concentration 50% ($EC_{50}$) of sediments ranged from 0.38 to 23.8 mg/mL. Most of the chemicals were present at levels lower than or comparable to the previously reported values from Korea. However, NP levels in the present study were in the high range of levels reported from the Korean coast, and 40% of the measured samples exceeded screening and ecotoxicological values of NP suggested by the Netherlands and Canada. This suggests that an ongoing source of NP is a serious concern in the Gunsan coast. High levels of contaminants were found in the proximity of potential sources, such as the outfall of a wastewater treatment plant for NP, an anthracite-fired power plant for PAHs, and ports for BTs, fecal sterols, and sediment toxicity. This indicates that Gunsan coast has various potential sources of marine sediment contaminants.

• Korean Journal of Food Science and Technology
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• v.11 no.2
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• pp.105-111
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• 1979

Phycomyces sp. has been isolated from Meju and its lipid composition was investigated. The lipid content was 18.2 % of dry weight and composed of 69.2 % neutral lipids. 24.3 % glycolipids and 6.5 % phospholipids. It was possible to identify the presence of carotene, hydrocarbons, esterified sterols, fatty acid esters, triglycerides. free fatty acids and free sterols in the neutral lipid fraction. Major components in the neutral lipid fraction were free fatty acids and triglycerides. Twelve molecular species of triglycerides and 8 molecular species of diglycerides were fractionated by argentation TLC. The major fatty acids in total lipids were stearic, linoleic, arachidic and linolenic acids, and those in the free fatty acid fraction were stearic, linoleic and arachidic acids.

• Journal of Nutrition and Health
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• v.27 no.2
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• pp.127-140
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• 1994

The effect of milk in low and high cholesterol diet was invesigated on serum cholesterol metabolism and lipid contents of serum, aorta, liver of rats. Weanling male Sprague-Dawley rats were divided into low(0.01% w/w) and high(1.01% w/w) cholesterol-diets groups. Low cholesterol groups subdivided into four groups ; control group was given water and three milk groups were given low heat milk(LM), ultra-high heat milk(HM), and powder milk(PM), respectivily, instead of water. High cholesterol groups were consisted of three groups ; control, LM, and HM groups. After feeding these experimental diets for six weeks, lipid levels were measured in serum and tissure and dried feces were analyzed for neutral and acidic sterols. Results obtained from this study are as follows : 1) Nutrient intakes, body weight gains and aorta weights did not differ among groups, but liver weights were higher in high cholesterol fed rats than low cholesterol fed rats. 2) Serum protein contents were increased independently by intakes of high cholesterol and milk. 3) Serum total cholesterol and triglyceride levels were increased but phospholipid levels and HDL-cholesterol/total-cholesterol ratios were decreased by high cholesterol in diet. And milk supplementation decreased serum total cholesterol and triglyceride levels but increased phospholipid levels and HDL-cholesterol/total-cholesterol ratios. 4) Contents of cholesterol and triglyceride in aorta and liver were elevated by dietary high cholesterol and lowered by consumption of all three types of milk. 5) Levels of cholesterol and triglyceride among serum, aorta and liver were highly correlated (r=0.7-0.9, p<0.001). 6) Fecal excretion of total sterols was three times high in high cholesterol group, compared with low cholesterol groups and were increased about 20% by milk consumption. 7) The effects of milk were more pronouncely shown in low cholesterol groups and mostly confined to LM and HM groups, rarely shown in PM group. It is concluded from the present study that milk had the hypolipidemic as well as hypocholes terolemic effect, which appears to be mediated through increased fecal bile acid excretion. But the effect is likely to be shaded by excess consumption of dietary cholesterol and was almost absent in powder milk.

• Journal of Apiculture
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• v.34 no.4
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• pp.315-323
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• 2019

Large amounts of sludge produced by paper mill industries represent one of the most serious environmental problems in the world. Recently, beekeepers living in the neighborhood of the paper mill in Hwasan County, Youngcheon city, GB, Korea, became alarmed that honey bee colonies were dying off suddenly across the neighborhood. A preliminary study was conducted to evaluate the toxicity (oral, fumigation, repellent) of recycled solid paper mill sludge (SPMS) and leachate paper mill sludge (LPMS) to honey bee workers under laboratory conditions, and to analyze the volatile organic compounds(VOC). The SPMS and LPMS were separately subjected to a liquid-liquid extraction (LLE) at three temperatures to extract VOC(highest VOC yields: 1.52% SPMS and 0.34% LPMS). A total of 70 chemicals were detected in the VOC of paper mill sludges, of which 49 and 21 volatile organic compounds from SPMS and LPMS, respectively. The SPMS was dominated by high degree presence of stanols (saturated sterols), such as cholestanol, cholestan-3-ol and also saturated hydrocarbons. However, LPMS was characterized by the absence of sterols. Both SPMS and LPMS showed an influence on the olfactory behavior of honey bee on Y-tube assay, with repulsion rates of 72 and 68%, respectively. Both SPMS and LPMS at concentration of 100mg/mL caused higher honey bee oral mortality than the untreated controls at 48, 72, 96 and 120 hours after treatment(highest oral mortality at 120 hr: 85.74%(SPMS); 93.51 % (LPMS)). A similar pattern was observed when honey bees were tested to fumigant toxicity. Both SPMS and LPMS caused significant higher mortality than the untreated control 24 hour after the exposure (highest fumigation mortality at 120 hr: 69.4% (SPMS); 56.8% (LPMS)). These preliminary results indicated that paper mill sludge could be partly responsible for sudden death and disappearance of honey bees, especially in hot humid summer days. With climate change, the risk of environmental chemical exposure to honey bee would pose greater attention.

• Natural Product Sciences
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• v.13 no.3
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• pp.247-250
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• 2007

A new sphingosine (1) was isolated from the MeOH extract of a marine sponge Haliclona (Reniera) sp. by bioactivity-guided fractionation. The 1D and 2D NMR, and MS spectroscopic analyses were used to establish the planar structure of 1. The stereochemistry of the compound was defined on the basis of modified Mosher's method, comparison of optical rotation and NMR data with those of the reported. Compound 1 was mildly cytotoxic to a panel of five human solid tumor cell lines.