• Journal of Microbiology and Biotechnology
• /
• 제18권10호
• /
• pp.1672-1677
• /
• 2008

IscR (iron-sulfur cluster regulator) has been reported to be a repressor of the iscRSUA operon, and in vitro transcription reactions have revealed that IscR has a repressive effect on the iscR promoter in the case of [$Fe_{2}S_{2}$] cluster loading. In the present study, the iscR gene from A. ferrooxidans ATCC 23270 was cloned and successfully expressed in Escherichia coli, and then purified by one-step affinity chromatography to homogeneity. The molecular mass of the IscR was 18 kDa by SDS-PAGE. The optical and EPR spectra results for the recombinant IscR confirmed that an iron-sulfur cluster was correctly inserted into the active site of the protein. However, no [$Fe_{2}S_{2}$] cluster was assembled in apoIscR with ferrous iron and sulfide in vitro. Therefore, the [$Fe_{2}S_{2}$] cluster assembly in IscR in vivo would appear to require scaffold proteins and follow the Isc "AUS" pathway.

• 한국윤활학회:학술대회논문집
• /
• 한국윤활학회 2002년도 proceedings of the second asia international conference on tribology
• /
• pp.289-290
• /
• 2002

Stainless steel ball bearings are used in the control element drive mechanism and driving mechanisms such as step motor and gear boxes for the integral nuclear reactor, SMART. The bearings operate in pressurized pure water (primary coolant) at high temperature and should be lubricated with only this water because it is impossible to supply greases or any additional lubricant since the whole nuclear rector system should be perfectly sealed and the coolant cannot contain ingredients for bearing lubrication. Temperature of water changes from room temperature to about 120 degree Celsius and pressure rises up to 15MPa in the nuclear reactor. It can be anticipated that the frictional characteristics of the ball bearings changes according to the operating conditions, however little data are available in the literature. It is found that friction coefficient of 440C stainless steel itself does not change sharply according to temperature variation from the former research, and the friction coefficient is about 0.45 at low speed range. In this research frictional characteristics of the assembled ball bearings are investigated. A special tribometer is used to simulate the axial loading and the bearing operating conditions, temperature and pressure in the driving mechanism in the nuclear reactor. Highly purified water is used as lubricant ‘ and the water is heated up to 120 degree Celsius and pressurized to 15MPa. Friction force is monitored by the torque transducer.

• Structural Engineering and Mechanics
• /
• 제2권4호
• /
• pp.359-367
• /
• 1994

The stability behaviour of symmetrically laminated rectangular composite plates with loaded ends clamped and unloaded edges simply-supported, and subjected to uniform in-plane compression is investigated. A numerical and experimental investigation is presented in this contribution. The stacking sequence of the laminated glass/epoxy composite plates is symmetric about the middle surface and consists of 8-ply [0, 90, +45, -45]s lamination. Numerical predictions were obtained through the use of the finite element method. The above plates were modelled with 8-noded isoparametric layered shell elements. The effect of the input parameters such as the degree and forms of prescribed initial imperfection and the incremental step size required for incremental loading, on the convergence of the solution is thoroughly examined. Experimental results are presented for 10 test panels. All test panels were made from glass/epoxy unidirectional prepregs and have aspect ratio of 5.088. The laminate thicknesses were found to vary from 1.054 mm to 1.066 mm. Comparison of experimental data with predicted results show good correlation and give confidence in the finite element model.

• Steel and Composite Structures
• /
• 제29권3호
• /
• pp.333-348
• /
• 2018

An analytical method is developed for analysing the contact buckling response of infinitely long, thin corrugated plates and flat plates restrained by a Winkler tensionless foundation and subjected to linearly varying in-plane loadings, where the corrugated plates are modelled as orthotropic plates and the flat plates are modelled as isotropic plates. The critical step in the presented method is the explicit expression for the lateral buckling mode function, which is derived through using the energy method. Simply supported and clamped edges conditions on the unloaded edges are considered in this study. The acquired lateral deflection function is applied to the governing buckling equations to eliminate the lateral variable. Considering the boundary conditions and continuity conditions at the border line between the contact and non-contact zones, the buckling coefficients and the corresponding buckling modes are found. The analytical solution to the buckling coefficients is also expressed through a fitted approximate formula in terms of foundation stiffness, which is verified through previous studies and finite element (FE) method.

• 환경위생공학
• /
• 제14권3호
• /
• pp.130-138
• /
• 1999

The feasibility of municipal sewage sludge digestion was investigated by using thermophilic anaerobic sequencing batch reactor(ASBR). One-day settle time was enough for the high performance of solid-liquid separation. The conversion of semi-continuous mode to sequencing batch mode is easily achieved without any adverse effects, although the large amount of sludge equal to the volume ratio of 0.3~06 to reactor volume was added in the feed step of the start-up. The ASBRs had higher conversion capability of organics to biogas than the control reactor. Gas yields of the ASBRs were increased by the average of 50% over the control reactor across a range of hydraulic retention time(HRT)s from 10days to 5days. The thermophilic reactors showed higher gas production than mesophilic reactor. Removal efficiencies of organic matter exceeded 80% on the basis of supernatants, except that at the reactor. Solid-liquid separation was essential in the performance of the ASBR, especially, at the lower HFT. The ASBRs were highly efficient in the retention of activated biomass within the reactor. thus compensating for increased equivalent organic loading rate through increased solids retention times followed by the increased solids, while maintaining shorter HRTs.

• 대한기계학회논문집A
• /
• 제21권3호
• /
• pp.378-384
• /
• 1997

In curing process of tire, contact and slip occurs between green tire and curing bladder. The curing process is a critical step in the manufacture of tires. In this investigation, curing bladder shaping is examined using a finite element method. Specifically, a finite element model between the inner part of green tire and the outer part of curing bladder is generated using contact element and curing bladder is generated using incompressible element. Numerical analysis are performed on two different bladder types, different overall outer diameters of curing bladder and different heights of curing bladder. Numerical results show that contact pressure is increased by using toroidal type of curing bladder, increasing overall diameter and increasing height of curing bladder. To obtain natural equilibrium carcass line, there is a requirement in increasing contact pressure of the section between side and bead.

• 대한기계학회논문집A
• /
• 제28권1호
• /
• pp.48-54
• /
• 2004

Generally, structural optimization is carried out based on external static loads. All forces have dynamic characteristics in the real world. Mathematical optimization with dynamic loads is extremely difficult in a large-scale problem due to the behaviors in the time domain. In practical applications, it is customary to transform the dynamic loads into static loads by dynamic factors, design codes, and etc. But the optimization results with the unreasonably transformed loads cannot give us good solutions. Recently, a systematic transformation has been proposed as an engineering algorithm. Equivalent static loads are made to generate the same displacement field as the one from dynamic loads at each time step of dynamic analysis. Thus, many load cases are used as the multiple loading conditions which are not costly to include in modem structural optimization. In this research, the proposed algorithm is applied to the optimization of flexible multibody dynamic systems. The equivalent static load is derived from the equations of motion of a flexible multibody dynamic system. A few examples that have been solved before are solved to be compared with the results from the proposed algorithm.

• 한국정밀공학회지
• /
• 제33권3호
• /
• pp.199-205
• /
• 2016

A thin sheet of metal with corrugated structures has been utilized in various devices: heat exchangers, separators in fuel cells, and many others. However, it is not easy to fabricate thin corrugated structures using a single-step stamping process due to their geometrical complexity. To solve this problem, firstly, a plate type heat exchanger was redesigned to attain the optimal value of aspect ratio and the optimal shape of corrugated structures for the actual loading conditions. A forming analysis of the corrugated plate was then carried out to determine the process parameters. From this work, the optimal value of aspect ratio was found to be 4.6. In addition, the process parameters of heat exchanger forming were optimized using the optimal value of aspect ratio, and the analytical results were evaluated through experiments. The results obtained indicated good agreements between them.

• 한국강구조학회 논문집
• /
• 제11권2호통권39호
• /
• pp.191-199
• /
• 1999

본 연구는 중층철골 구조물 공업화방안에 관한 연구의 기초단계로서 앵글과 고력볼트를 사용한 콘크리트충전 각형강관기둥 H형강보 접합부의 구조적 거동을 파악하는데, 그 목적이 있다. 실험은 앵글의 두께를 주요변수로 하여 정적하중 하에 5종류의 시험체에 대해 실행되었다. 내력추정식은 접합부의 강도를 상계정리에 의한 하한값으로 구하는 항복선 이론을 적용하여 제시하였다.

• Structural Engineering and Mechanics
• /
• 제32권4호
• /
• pp.501-516
• /
• 2009

This paper aims to study the large deflections of variable-arc-length elastica subjected to the terminal forces (e.g., axial force and torque). Based on Kirchhoff's rod theory and with help of Euler parameters, the set of nonlinear governing differential equations which free from the effect of singularity are established together with boundary conditions. The system of nonlinear differential equations is solved by using the shooting method with high accuracy integrator, seventh-eighth order Runge-Kutta with adaptive step-size scheme. The error norm of end conditions is minimized within the prescribed tolerance ($10^{-5}$). The behavior of VAL elastica is studied by two processes. One is obtained by applying slackening first. After that keeping the slackening as a constant and then the twist angle is varied in subsequent order. The other process is performed by reversing the sequence of loading in the first process. The results are interpreted by observing the load-deflection diagram and the stability properties are predicted via fold rule. From the results, there are many interesting aspects such as snap-through phenomenon, secondary bifurcation point, loop formation, equilibrium configurations and effect of variable-arc-length to behavior of elastica.