• Journal of Chest Surgery
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• 제21권3호
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• pp.583-587
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• 1988

Bilateral coronary artery-pulmonary artery fistula is very uncommon congenital heart disease which occupy small percentage of all coronary arterio-venous fistulas. We experienced a case who was 52 years old female with bilateral coronary artery-pulmonary artery fistula. She complained exertional dyspnea k angina[coronary steal syndrome]. On physical examination, any cardiac murmur was not audible. There was no 0y step-up in right heart catheterization. But selective coronary angiography revealed tortuous aberrant vessels which originated from the canal branch of the right coronary artery k the left anterior descending coronary artery. Both aberrant vessels traversed the right ventricular outflow tract, and conjoined just proximal the pulmonic annulus and drained into the main pulmonary artery. The operation was performed under the extracorporeal circulation with beating heart. The procedures were suture-ligation of the draining orifice in main pulmonary artery & the feeding vessels on the right ventricular outflow tract. Postoperatively her complaints were completely disappeared and the selective coronary angiography revealed no left-to-right shunt.

• 전기학회논문지
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• 제64권2호
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• pp.342-348
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• 2015

In this paper, fuel cell power generation system that is being studied in recent railway field was applied to compensate for the voltage drop due to the load as driving electric vehicle. PSIM simulation program is to be used to implement the modeling of the electric railway for AC AT feeder system. For it, It was applied to the product-type single-phase PLL algorithm, step-down converter is controlled as power so as to have the fuelcell generation system. Based on it's result, a reactive power due to the catenary impedance in accordance with the current flowing is compensated as linked with fuelcell generation system which supplied the current to the power supply grid. and then its performance was confirmed that voltage compensation effect obtained at SubStation (SS), SubSectioning Post (SSP), Sectioning Post (SP).

• 한국공작기계학회논문집
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• 제18권2호
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• pp.207-213
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• 2009

The bundling process is the final step in vegetable manufacturing, however, the process is a little difficult to be automatized, because vegetable has the physical properties of roughness, softness, and fragility etc. In this paper, we proposed an automatic bundling mechanism for vegetable based on the heat melt sticking. The proposed mechanism consists of three modules, one module is the moving part for aligning of the vegetable shape and adjusting of the vegetable tension, second module is the arm driving part for the vegetable binding and the band roll releasing, and third module is band joining, band cutting, and band feeding part for the vegetable binding continuously. Through this research, Using the SMO(SimDesigner Motion) module, we optimize condition of mechanical movement of the bundling mechanism. This bundling system designed in order to binding 288 bundle/hour.

• Journal of Microbiology and Biotechnology
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• 제20권3호
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• pp.622-629
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• 2010

Biosynthetic studies on brasiliamides, potently convulsive and bacteriostatic compounds from an endophytic Penicillium brasilianum isolated from Melia azedarach (Meliaceae), confirms their phenylpropanoid origin, which is very uncommon in fungi. Feeding experiments with [$2-^{13}C$]-phenylalanine indicated the incorporation of two units of this amino acid on brasiliamide structures. The first step in the phenylpropanoid pathway to those compounds was evaluated through enzymatic bioassays and confirmed the phenylalanine ammonia-lyase (PAL) participation. The metabolism of phenylalanine in this fungus is discussed.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권5호
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• pp.303-305
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• 2003

Scale-up of hirudin production from Saccharomyces cerevisiae from bench-scale to pilot-scale was carried out based on constant volumetric oxygen transfer coefficient (K$\sub$L/a). Fed-batch mode of cultivation using step-wise feeding strategy of galactose was employed for the production of hirudin in a 30-L and a 300-L pilot-scale fermentor. The final hirudin concentrations were achieved 390 mg/L and 286.1 mg/L, and the volumetric productivities were 80.4% and 90.7% with the 30-L and 300-L fermentors, respectively, compared to the productivity of the 5-L bench-scale fermentor.

• 한국식물학회:학술대회논문집
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• 한국식물학회 2002년도 춘계학술발표대회:발표눈문요지록
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• pp.73-82
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• 2002

The committing step in Met and S-adenosyl-L-methionine (SAM) synthesis is catalyzed by cystathionine ${\gamma}$ -synthase (CGS). Transgenic Arabidopsis thaliana overexpressing CGS under control of 35S promoter show increased soluble Met and its metabolite S-methylmethionine, but only at specific stages of development. CGS-overexpressing seedlings are resistant to ethionine. Similar results were obtained with transgenic potato plants overexpressing Arabidopsis CGS. Several of the transgenic lines show silencing of CGS resulting in deformed p]ants with a reduced capacity for reproductive growth similar as transgenic plants by antisense RNA (CGS[-]). Exogenous feeding of Met to the CGS[-] and CGS[+] silenced plants partially restores their growth. Similar morphological deformities are observed in plants cosuppressed for SAM synthetase, even though such plants accumulate 250 fold more soluble Met than wild type and they overexpress CGS. The results suggest that the abnormalities associated with CGS and SAM synthetase silencing are due in part to a reduced ability to produce SAM, and that SAM may be a regulator of CGS expression.

• Transactions on Electrical and Electronic Materials
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• 제15권1호
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• pp.12-15
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• 2014

At the moment of transformer energization by the supply voltage, a high current called transient inrush current, which may rise to ten times the transformer full load current, could be drawn by the primary winding. This paper discusses a microcontroller circuit with the intention of controlling and limiting the inrush current for a transformer, by the method of ramping up the supply voltage feeding to the transformer primary. Simulations and the experimental results show a significant reduction of inrush current, when the ramping up voltage is applied to the three-phase transformer load. The inrush current could be almost eliminated if the correct switching step rate is chosen.

• 한국자동차공학회논문집
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• 제16권1호
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• pp.86-92
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• 2008

Recently, the use of tubes in the manufacturing of the automobile parts has increased and therefore many automotive manufactures have tried to use hydro-forming technology. The hydro-forming technology may cause many advantages to automotive applications in terms of better structural integrity of the parts, lower cost from fewer part count, material saving, weight reduction, lower spring-back, improved strength and durability and design flexibility. In this study, the whole process of front engine cradle (or front sub-frame) parts development by tube hydro-forming using steel material having tensile strength of 440MPa grade is presented. At the part design stage, it requires feasibility study and process design aided by CAE (Computer Aided Design) to confirm hydro-formability in details. Effects of parameters such as internal pressure, axial feeding and geometry shape on automotive sub-frame by hydro-forming process were carefully investigated. Overall possibility of hydro-formable sub-frame parts could be examined by cross sectional analyses. Moreover, it is essential to ensure the formability of tube material on every forming step such as pre-bending, preforming and hydro-forming. At the die design stage, all the components of prototyping tools are designed and interference with press is examined from the point of geometry and thinning.

• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1327-1338
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• 2013

The humanized anti-hepatocyte growth factor (HGF) monoclonal antibody (mAb) YYB-101 is a promising therapeutic candidate for treating various cancers. In this study, we developed a bioprocess for large-scale production of YYB-101 and evaluated its therapeutic potential for tumor treatment using a xenograft mouse model. By screening diverse chemically defined basal media formulations and by assessing the effects of various feed supplements and feeding schedules on cell growth and antibody production, we established an optimal medium and feeding method to produce 757 mg/l of YYB-101 in flask cultures, representing a 7.5-fold increase in titer compared with that obtained under non-optimized conditions. The optimal dissolved oxygen concentration for antibody production was 70% $pO_2$. A pH shift from 7.2 to 7.0, rather than controlled pH of either 7.0 or 7.2, resulted in productivity improvement in 5 L and 200 L bioreactors, yielding 737 and 830 mg/ml of YYB-101, respectively. The YYB-101 mAb highly purified by affinity chromatography using a Protein A column and two-step ion exchange chromatography effectively neutralized HGF in a cell-based assay and showed potent tumor suppression activity in a mouse xenograft model established with human glioblastoma cells.

• 한국자동차공학회논문집
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• 제16권3호
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• pp.38-43
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• 2008

The hydroforming technology has been spreaded dramatically in automotive industry last 10 years. Itmay cause many advantages to automotive applications in terms of better structural integrity of the parts, lower cost from fewer part count, material saving, weight reduction, lower springback, improved strength and durability and design flexibility. In this study, the whole process of rear sub-frame parts development by tube hydroforming using steel material having tensile strength of 440MPa grade is presented. At the part design stage, it requires feasibility study and process design aided by CAE (Computer Aided Design) to confirm hydroformability in details. Effects of parameters such as internal pressure, axial feeding and geometry shape in automotive rear sub-frame by hydroforming process were carefully investigated. Overall possibility of hydroformable sub-frame parts could be examined by cross sectional analyses. Moreover, it is essential to ensure the formability of tube material on every forming step such as pre-bending, preforming and hydroforming. In addition, all the components of prototyping tool are designed and interference with press is examined from the point of geometry and thinning.