Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Scale-up of Recombinant Hirudin Production from Saccharomyces cerevisiae

  • Kim, Chul-Ho (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • K. Jagannadha Rao (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Youn, Duk-Joong (Dong Kook Pharmaceutical Co. Ltd.,) ;
  • Rhee, Sang-Ki (Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2003.10.01
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Abstract

Scale-up of hirudin production from Saccharomyces cerevisiae from bench-scale to pilot-scale was carried out based on constant volumetric oxygen transfer coefficient (K$\sub$L/a). Fed-batch mode of cultivation using step-wise feeding strategy of galactose was employed for the production of hirudin in a 30-L and a 300-L pilot-scale fermentor. The final hirudin concentrations were achieved 390 mg/L and 286.1 mg/L, and the volumetric productivities were 80.4% and 90.7% with the 30-L and 300-L fermentors, respectively, compared to the productivity of the 5-L bench-scale fermentor.

Keywords

References

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