• Korean Journal of Plant Resources
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• v.29 no.1
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• pp.46-61
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• 2016

The ethnobotanical study was conducted to record and conserve the traditional knowledge of botanical taxa in Hallyeo-Haesang National Park area, S. Korea from 2012 to 2013. According to the survey results, derived from 1,430 sheets of 183 residents at 70 places, the ethnobotanical plants in Hallyeo-Haesang National Park area consisted of a total 247 taxa; 82 families, 197 genera, 224 species, 3 subspecies, 19 varieties and 1 form. The analysis of usage for 247 taxa showed that the edible use was the highest with 166 taxa, followed by medicinal with 138 taxa, craft with 27 taxa, material with 16 taxa and ritual with 9 taxa respectively. The leaf of plant was the most useful part, followed by stem and fruit plant. Quantitative analysis of the ethnobotanical plants in Hallyeo-Haesang National Park area was performed by the basic values of FC, NU and UR, and indices of CI, RFC, RI and CV. As a result, The CI places Artemisia princeps in the first position, followed by Pueraria lobata, Taraxacum platycarpum. The RFC place A. princeps in first position, followed by Achyranthes japonica, Aster scaber. The RI places A. princeps in the first position, followed by P. lobata, Pinus densiflora. The CV places A. princeps in the first position, followed by P. lobata, A. japonica. Farfugium japonicum had been used as specific ethnobotanical plant in this area, for arthralgia, postpartum arthrodynia, circulatory disturbance.

• Journal of Korean Society of Forest Science
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• v.104 no.4
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• pp.536-542
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• 2015

This study was examined for the growth response of tree diameter and volume to thinning treatments from different thinning intensities using three long-term thinning trials for Japanese Evergreen Oak (Quercus acuta Thunb.) stands in Wando island, Korea. After thinning in 1999, annual tree growth of diameter and volume was highest in heavy thinned stands for individual tree and this growth pattern of thinning response showed similar tendency to the individual tree growth response in light thinned stands. By increasing diameter growth, the value of H/D ratio (HDR) as an indicator of stem form was properly decreased and improved up to 80%. Although there is significant growth response of basal area in both heavily and lightly thinned stands, the growth potential both of heavily and lightly thinned stands in total stand volume is not likely to reach at the level of unthinned stands because of basal area growth loss associated with both light and heavy thinnings.

• Korean Journal of Environment and Ecology
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• v.20 no.4
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• pp.425-435
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• 2006

The objectives of this research were to investigate the drought resistance as well as the growth of Wisteria floribunda seedlings with the soil water conditions. The seedlings for the research were grown in pots with strict water content control on a frame located outdoors. During the experiments, the soil water contents were adjusted to 5, 10, 20, 30, 40, 50, 60, and 70%. In addition, the effects of Rhizobium inoculation on the growth of seedlings were investigated. The experimental results are summarized as follows: 1. The seedlings in the pots with 5% soil water content withered to death due to the water stress. Withering or any distinct growth was not observed from the seedlings in the 10% soil water content. It can be inferred from these results that about 5% of soil water content is the wilting point of W. floribunda seedlings and about 10% is the critical soil water content of its growth in this experiment soils. Therefore, it seems that W. floribunda possesses a good drought resistance. 2. From the analyses of the main growth parameters such as stem elongation, diameter growth, leaf area growth and total dry weight, it was found that the seedling growth can be improved with increasing soil water contents. The relation between each growth parameter(Y) and the soil water contents(W) was well described by a quadratic equation, $Y=a+bW+cW^2$. 3. In soil water contents higher than 20%, the seedling growth(Y) was remarkable along with-its extended growing period, and related to the growing period(D) by a quadratic equation, $Y=a+bD+cD^2$. 4. The artificial inoculation of Rhizobiun promoted the growth of Wisteria floribunda seedlings. 5. Rhizobium was found to be more readily inoculated and to form more root nodules compared to seedlings grown in higher soil water contents.

• Korean Journal of Plant Tissue Culture
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• v.25 no.2
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• pp.135-139
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• 1998

Reciprocal interspecific hybrids between N. glauca(2n=24) and N. langsdorffii(2n=18) were obtained by intercrossing. One hundred percent of F$_1$ seeds was produced from intercrossing of N. glauca $\times$ N. langsdorffii, whereas the frequency of F$_1$ hybrid seed formation from N. langsdorffii $\times$ N. glauca was very low. However, all the hybrid seeds were germinated well and then grown to normal plantlets. All the plants of F$_1$ hybrids have chromosome number of interspecific hybrids (2n=21). From observation of morphological characteristic, the structure of petrol, leaf, flower, and the morphology of pollen have characteristics of F1 hybrid. Spontaneous tumors (genetic tumor) were formed from each F$_1$ hybrid; the genetic tumor arose at the reproductive phase when the maternal type of F$_1$ hybrid came from N. glauca, while the genetic tumor arose only after reproductive phase when the maternal type of F$_1$ hybrid came from N. langsdorffii. The genetic tumor actively proliferated on hormone-free medium and produced numerous teratoma shoots. In addition, normal leaf or stem explants of F$_1$ hybrid produced calli on hormone-free medium after 15 days of culture, the calli produced new numerous teratoma shoots after 30 days. The frequency of teratoma shoot formation from rnterspecific hybrid was higher in the N. glauca $\times$ N. langsdorffii than in the N. langsdorffii $\times$ N. glauca. Root development from the teratoma shoots was hardly obtained. Teratoma shoots without roots in vitro can form genetic tumor at the vegetative growth phase after tissue culture.

• Journal of Korean Society of Forest Science
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• v.34 no.1
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• pp.1-14
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• 1977

These studies were carried out to know the variation of morphological tree forms, needles, cotyledons, seed characters and top height growth of seedlings of Pinus koraiensis S. et Z. by populations in Mt. Jiri. The results are summarized as follows: 1. It was observed that the characteristics of length, needles of serration density of needles, percentage of full seeds length, of seeds, thickness, of seeds length of cotyledons, width of cotyledon, numbers of the stomata line in cotyledons and the top height growth of seedlings were significantly different in individual trees and populations. 2. The characteristics of branch angle specific gravity of wood and resin canal numbers of cotyledons were significantly different in populations. 3. It was that the characteristics of numbers of stomata line, width, of seeds weight, of seeds serration density of cotyledons, numbers of cotyledons were significantly different in individuals. 4. The resin canals of needles showed three principle resin canals in individual trees and population, and the coefficients of variation were not shown in individual trees and populations. Stem forms showed to be a straight form with all populations. 5. The characteristics of cotyledons had generally higher tendency than the characteristics of needles in comparison with coefficience of variation.

• FLOWER RESEARCH JOURNAL
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• v.19 no.4
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• pp.264-268
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• 2011

'Duo', a late-flowering white gladiolus with pink core, was released by the National Institute of Horticultural & Herbal Science (NIHHS) in 2000. The selection was derived from the cross between the progenies of the early flowering dark pink 'True Love' and bright pink domestic cultivars 'Madame Valdiek'. The breeding was conducted in 1996 and selected finally in 2000 after four years (1997 to 2000) of experimentation. 'Duo' is a cut flower with bright white color petals and Pink core which exhibited late flowering and vigor without stem bending. It can be cultivated in areas or regions with strong winds during summer. 'Duo' has a very firm growth form and sturdy branches which do not bend when planted in the open fields. Likewise, the plant showed high tolerance to flooding and resistance, hence, could grow vigorously in open areas. It is a fast- growing plant which exhibits high corm production rate. 'Duo' had a 12.2 cm wide flower and 150 cm plant height. During summer, the average number of days to flowering was recorded at 75 days of 'Duo'. Top dressing must be conducted once or twice during corm production to enhance corm quality and quantity.

• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.76-81
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• 2012

Silk protein has been explored to be used for biomedical applications for several decades. However, it has not been used in this field cause to their irreversible crystallization after dissolving in water. The existing methods of silk protein hydrolysis using silkworm cocoon were used with harmful solvents and through a very complicated process. Therefore, we have developed novel methods for the production of water-soluble hydrolysate using silkworm gland. We manufactured two types of silkworm gland-derived hydrolysate (water-soluble SGH, SSGH; total SGH, TSGH) and compared the characteristics with commercial cocoon-derived sericin hydrolysate (CSH). The molecular weight of SGH ranged from 7 to 50 kDa (SSGH) and 5 to 15 kDa (TSGH) within glycine, alanine, and aspartic acid as a main amino acid composition. In contrast, CSH ranged from 15 to 50 kDa within serine and aspartic acid. The results of FTIR implied that SGH was more soluble form than CSH, as shown by the decrease in the ${\beta}$-sheet structure at $1630cm^{-1}$ on amide I peak. In comparison with 10% fetal bovine serum, 0.1% (1 mg/ml) SSGH had equivalent effect on the proliferation of human dermal fibroblasts and mesenchymal stem cells. All results of the SSGH made by novel manufacturing process indicate the SSGH is more preferable as a culture medium supplement than cocoon-derived sericin.

• Journal of The Korean Association For Science Education
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• v.39 no.3
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• pp.321-335
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• 2019

This study aims to explore the influencing factors and the process of enhancing science self-efficacy (SSE) and to lay the foundation in understanding science self-efficacy of students. The ten categories related to the science self-efficacy were derived through the coding of the interview data based on the grounded theory and paradigm analysis to develop a process model of science self-efficacy improvement. Through the process analysis, four cyclical phases were found in the process of enhancing SSE: 'Entering into learning science' phase, 'enhancing SSE' phase, 'adjustment' phase, and 'result' phase. More specifically, the phase of 'entering into learning science' is where students choose science track and stimulated to construct SSE. The phase of 'enhancing SSE' is where students taking a science track actively learn science and perform science activities. In the phase of 'adjustment', students come to have successful performance about learning science and performing science activities by using diverse strategies. Finally, 'result' phase indicates different appearances of students depending on SSE levels. The phases were non-linear and periodically repeat depending on situation. The core category in the selective coding was indicated to be 'enhancing science self-efficacy.' Students' SSE form by learning science and performing science activities. These finding may help better understand the behavior of students who are taking a science track by facilitating effective science learning through the increase of their SSE levels.

• Microbiology and Biotechnology Letters
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• v.47 no.1
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• pp.164-171
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• 2019

Radiation therapy has many side effects, such as digestive mucosal ulcers, without regard to its efficacy. The purpose of this study is to address an alternative method to replace the limitation of radiation therapy using radiomimetic microbial ribotoxins. In the evaluation of cancer therapy, we analyzed the formation of colorectal cancer (CRC) cell spheroids, which can take into account the heterogeneous cellular constitution, tumor stem cells, and the surrounding microenvironment. Ribotoxic stress interfered with the spheroid structure composed of relatively small clusters. Spheroids under ribotoxic stress were structurally sparse and their shrinkage was very slow. In the control group, the clusters of strongly aggregated cells were resistant to physical stress, but the ribotoxic stress-exposed spheroids were easily broken up by the physical stress. Moreover, the ribosome-insulted CRC cells slowly migrated to form clusters and the cell-cell junctional points in the ribosome-insulted spheroids were rarer than those in the control CRC spheroid. Moreover, levels of the cell-to-cell junctional protein E-cadherin were suppressed by ribotoxic stress in both allograft and xenograft spheroids. In conclusion, the radiomimetic microbial ribotoxins induced structural defects in CRC cell spheroids via retardation of migration and cell-cell junction in the formation of three-dimensional structures, and provides a basis for the mechanism of pharmacological radiomimetic anticancer actions as an alternate to radiotherapy against cancer.

• Journal of Naturopathy
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• v.12 no.1
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• pp.7-15
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• 2023

Background: As a result of analyzing the components of wild Conyza canadensis, it contains physiologically active ingredients, so it is necessary to identify the compound. Purposes: It was to study the compound's molecular structure; a previous study showed that C. canadensis contains antioxidant substances. Methods: The ultrasonic pulverized lysate of C. canadensis stem and leaves was first extracted with 90% methanol and then five organic solvents. Next, the extracts was fractionated by HPLC, LC/MS chromatography, and NMR analyzers identified the molecular structure. Results: 100 g of dry C. canadensis was sonicated in 90% methanol and concentrated under reduced pressure to 11.96 g of a crude extract. Then, this crude was extracted with five types of solvents to obtain 123.8 mg of n-hexane, 448.2 mg of dichloromethane, 1047.7 mg of ethyl acetate (EA), 2563.8 mg of butanol, and 7.04 g of water. The EA extracts were fractionated by LC-MS and then re-fractionated to obtain F1 to F20. Next, the F15 was further fractionated to obtain nine fine fractions. Finally, the F17 fraction was re-fractionated to obtain ten fine fractions. As a result of LC-MS and NMR spectrometer analysis of the F15-7, the structure of this compound was confirmed as 3,5-dicaffeoylquinic acid. As a result of examining the structures of the F17-4 and F17-5 fractions, Quercetin-3-o-β-galactose was identified. In addition, the form of the F17-10 was confirmed to be 1,3,4-tri-caffeoylquinic acid. Conclusions: This study demonstrated that C. canadensis contained phenolic antioxidants, and its utilization may be expected.