Journal of Naturopathy (한국자연치유학회지)

Koean Soceity for Naturopathy (한국자연치유학회)
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Isolation and Structural Identification of Antioxidant Substances from Ethyl Acetate Extract of Conyza canadensis

망초(Conyza canadensis) Ethyl Acetate 추출물의 항산화성 물질의 분리와 동정

  • Hyun Sook Song (Dept. of Naturopathy, Graduate School of Integrative Medicine, Sun Moon University)
  • 송현숙 (선문대학교 통합의학대학원 자연치유학과)
  • Received : 2023.03.02
  • Accepted : 2023.03.04
  • Published : 2023.03.31
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Abstract

Background: As a result of analyzing the components of wild Conyza canadensis, it contains physiologically active ingredients, so it is necessary to identify the compound. Purposes: It was to study the compound's molecular structure; a previous study showed that C. canadensis contains antioxidant substances. Methods: The ultrasonic pulverized lysate of C. canadensis stem and leaves was first extracted with 90% methanol and then five organic solvents. Next, the extracts was fractionated by HPLC, LC/MS chromatography, and NMR analyzers identified the molecular structure. Results: 100 g of dry C. canadensis was sonicated in 90% methanol and concentrated under reduced pressure to 11.96 g of a crude extract. Then, this crude was extracted with five types of solvents to obtain 123.8 mg of n-hexane, 448.2 mg of dichloromethane, 1047.7 mg of ethyl acetate (EA), 2563.8 mg of butanol, and 7.04 g of water. The EA extracts were fractionated by LC-MS and then re-fractionated to obtain F1 to F20. Next, the F15 was further fractionated to obtain nine fine fractions. Finally, the F17 fraction was re-fractionated to obtain ten fine fractions. As a result of LC-MS and NMR spectrometer analysis of the F15-7, the structure of this compound was confirmed as 3,5-dicaffeoylquinic acid. As a result of examining the structures of the F17-4 and F17-5 fractions, Quercetin-3-o-β-galactose was identified. In addition, the form of the F17-10 was confirmed to be 1,3,4-tri-caffeoylquinic acid. Conclusions: This study demonstrated that C. canadensis contained phenolic antioxidants, and its utilization may be expected.

배경: 야생 망초(줄기와 잎)의 성분을 분석한 결과에서 생리활성 성분의 함유가 확인되어 화합물의 규명이 필요하다. 목적: 망초에는 항산화성 물질이 함유되었다는 선행연구 결과가 있어서 화합물의 분자적 구조를 연구하는 것이 목적이었다. 방법: 망초(줄기와 잎) 초음파 분쇄물을 1차로 90% 메탄올로, 다음에 유기 용매로 추출하였다. 다음에 HPLC, LC/MS 크로마토그래피 등으로 분획하였고, 분핵물을 NMR 분광기로 정밀 분석하여 분자의 구조를 동정하였다. 결과: 망초 100 g을 초음파기로 파쇄하여 90% methanol로 추출하고, 감압 농축하여 조 추출물 11.96 g을 얻었다. 조추출물을 유기용매로 재추출하여 n-hexane으로 123.8 mg, dichloromethane으로 448.2 mg, ethyl acetate(EA)로는 1047.7 mg, butanol로는 2563.8 mg, water로는 7.04 g을 얻었다. EA 추출물을 LC-MS 크로마토그래피로 분획하고, 정밀 분획하여 F1~F20의 20개를 얻었다. F15 분획을 다시 분획하여 9개를 얻었다. F17 분획을 재분획하여 10개의 분획을 얻었다. F15-7 분획물을 LC-MS 분석과 NMR 분광기로 분석한 결과는 이 화합물의 구조가 3,5-di-caffeoylquinic acid로 확인되었다. F17-4와 F17-5의 구조를 조사한 결과는 Quercetin-3-o-β-galactose로 동정 되었다. F17-10의 구조를 확인한 결과는 1,3,4-tri-caffeoylquinic acid로 확인되었다. 결론: 본 연구에서 망초 성분에도 항산화성 물질이 있었으며, 이의 활용성을 기대하며, 식물에는 항산화성 물질인 phenol 성분을 다양하게 함유하였다.

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References

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