• Korean Journal of Plant Resources
• /
• v.22 no.4
• /
• pp.287-292
• /
• 2009

Recently, the consumption of mistletoe[Viscum album var. coloratum(Kom.) Ohwi] is increasing because of its good medical effectiveness with the increased concern on the natural medicines and foods. The result obtained from the investigation on the stem tissues of the mistletoe and the oriental chestnut oak, a host plant species, are as follows. Haustorium from the seeds of the mistletoe after their sticking to the branches of the host plant penetrates into the bark where it forms the endophyte system through the active cell division. The endophyte grown in the cambium of the host plant makes the stems and leaves as the outer tissues in a certain time. Even through lignification of the host wood in the branches the oriental chestnut oak was not progressive, its tylosis coas developed partially assembly due to the formation of the endophyte. The stems of the mistletoe consisted of vascular tracheid, selereid, and ray and axial parenchyma, classified as a hardwood without vessels. The vascular tracheids seemed to take a role instead of the vessels in the mistletoe plant from the result that the pits of the vessels in the host branches are linked to the vessel-form tracheid in the mistletoe stems. The constituent ratio of the sclereid cells in the mistletoe stems increased with aging. Furthermore their ratio of the parenchyma cells was higher, which contained the more cell content, compared with the cells of the general woody plant species.

• The Korean Journal of Ecology
• /
• v.28 no.3
• /
• pp.163-168
• /
• 2005

This study was carried out to estimate above-ground biomass and inorganic nutrient distribution for a Camellia japonica stand located Mt. Cheon-gwan, Jeonnam province. Regression analysis of biomass for stem, current twig, branch and foliage versus diameter at breast height(DBH) was used to calculate regression equations of the form of logY = a + blogD(Y: component biomass, D: DBH). Total above-ground biomass for a Camellia japonica stand was 115.2 ton/ha(47.9 for main stem, 1.4 for current twig, 53.4 for live and dead branch, 5.6 for current foliage and 6.9 for ${\geq}1$-yr-old foliage). Component biomass was non-linearly correlated with DBH, and the difference in biomass between ${\geq}1$-yr-old and current foliage increased in proportion to DBH. Current foliage and live branch showed higher N, P and K concentrations compared to ${\geq}1$-yr-old foliage and dead branch, respectively. However, Ca concentration of current foliage and live branch was lower than that of ${\geq}1$-yr-old foliage and dead branch, respectively. Total above-ground inorganic nutrient contents(kg/ha) were distributed as follows; K: 366.4. N: 442.7, Ca: 433.3, Mg: 118.4, P: 50.5 and Na: 25.3. The proportions of inorganic nutrient content for live branch were generally the highest in all the inorganic nutrients.

• Journal of Mushroom
• /
• v.3 no.2
• /
• pp.71-74
• /
• 2005

The sterile form of Inonotus obliquus is used for preparation of the medicine befungin that is effective in the treatment of gastritis, gastric ulcers, and several tumors. The fungus is known to be produced mainly on the stems of Betula platyphylla var. japonica that grows at high altitudinal (above 1,100 m) region in Korea. But, we found the mushroom on the stem of Betula costata at Mt. Jumbong in Korea. We isolated a pure culture of the fungus from the stem of B. costata by use of potato dextrose agar (PDA) medium with streptomycin. We could isolate the fungus from plant's tissue filled with hyphae, but not from other parts. The spore collected from the sclerotia showed $6.0{\sim}10.0{\times}4.5{\sim}6.0{\mu}m$ in diameter, and the hypha was $2.5{\sim}5.0{\mu}m$ in thickness. The colony showed irregular features and scattered yellow color at the center as the culture ages. We could find brownish setae at the yellow region of colony at 20 days of culture, and the size ranged $4{\sim}6{\times}100{\sim}420{\mu}m$. The oatmeal agar (OA) provided best growth for I. obliquus among five media (CDA, CMA, MA, OA and PDA). Optimum temperature ranged $25{\sim}30^{\circ}C$, and optimum pH was relatively alkaline with the range of pH 8.0~9.5.

• Journal of Bio-Environment Control
• /
• v.3 no.1
• /
• pp.20-27
• /
• 1994

This study was conducted to improve marketing value and productivity of cucumber which was cultivated with primary scaffold stem type (Mannungcheongjang) and lateral stem type (Sayeup). The results are summarized as follows ; 1. Investigation of fruit setting characteristics to improve cucumber training type was resulted that the fruit-thinning was effective 3 nodes in Mannung- cheongjang and 4 nodes in Sayeup, because of defected yield potential and marketing value. 2. In the matter of early growing stage after training and cucumber quality at different treatment, cucumber weight at flowering curved cucumber and growth analysis of Mannungcheongjang were good in order of horizontal>vertical>slant training. And those of Sayeup were good in order of horizontal >slant> vertical training. 3. Aspect of light reception of cucumber plant in the level of plant height and accumulated leaf area index, vertical and horizontal training are better than slant in Mannungcheongjang, and there is order of horizontal >vertical >slant training in Sayeup. 4. Horizontal training in Mannungcheongjang was superior to any other case In view of cucumber number, productivity, and marketing rate. Therefore, this training was suggest ed of best method in cucumber cultivation.

• Journal of Microbiology and Biotechnology
• /
• v.26 no.11
• /
• pp.1881-1890
• /
• 2016

Manganese superoxide dismutase (MnSOD) is a vital enzyme that protects cells from free radicals through eliminating superoxide radicals ($O^{2-}$). Hirudin, a kind of small active peptide molecule, is one of the strongest anticoagulants that can effectively cure thrombus diseases. In this study, we fused Hirudin to the C terminus of human MnSOD with the GGGGS linker to generate a novel dual-feature fusion protein, denoted as hMnSOD-Hirudin. The hMnSOD-Hirudin gene fragment was cloned into the pET15b (SmaI, CIAP) vector, forming a recombinant pET15b-hMnSOD-Hirudin plasmid, and then was transferred into Escherichia coli strain Rosetta-gami for expression. SDS-PAGE was used to detect the fusion protein, which was expected to be about 30 kDa upon IPTG induction. Furthermore, the hMnSOD-Hirudin protein was heavily detected as a soluble form in the supernatant. The purification rate observed after Ni NTA affinity chromatography was above 95%. The hMnSOD-Hirudin protein yield reached 67.25 mg per liter of bacterial culture. The identity of the purified protein was confirmed by western blotting. The hMnSOD-Hirudin protein activity assay evinced that the antioxidation activity of the hMnSOD-Hirudin protein obtained was $2,444.0{\pm}96.0U/mg$, and the anticoagulant activity of the hMnSOD-Hirudin protein was $599.0{\pm}35.0ATU/mg$. In addition, in vitro bioactivity assay showed that the hMnSOD-Hirudin protein had no or little cytotoxicity in H9c2, HK-2, and H9 (human $CD_4{^+}$, T cell) cell lines. Transwell migration assay and invasion assay showed that the hMnSOD-Hirudin protein could suppress human lung cancer 95-D cell metastasis and invasion in vitro.

• Korean Journal of Medicinal Crop Science
• /
• v.1 no.2
• /
• pp.171-177
• /
• 1993

This experiment was conducted to select and rear the adaptable cultivar which isused for various purpose and to build a year-round cultivation using with characters of aerial part and analysis of chemical components in 28 local cultivar of Artemisia princeps Var. The growth of aerial part, which are stem length, stem diameter, no. of branch, node, leaf size and leaf pubescence, in north local cultivars was more vigorous than those of south local caltivars. Fresh weight of "Myeongcheonssuk " was highest in local caltivars, and in view of local area, local cultivars which was collected from Iri Si and Euiseong Gun was shown the highest fresh weight. Local cultivars as mentioned above had a characters with plenty of leaves, nodes and pubescence of leaf is long. In chemical components of leaves in morth cultivar, content of N, MgO, Zn, Cu, Mn was high, while $P_2O_5,\;K_2O$ in middle local cultivars, Fe in south local cultivars. The more content of carbohydrate in leaves was high, the content of $K_{2}O$ had a increasing tendency. In local cultivars which was collected form Iri Si and Euiseong Gun with the highest fresh weight, content of $N,\;P_{2}O_5,\;K_{2}O$ was high, on the other hand content of MgO, Zn, Ca, Mn, Fe, was low in leaves of Artemisia Princeps Var.

• Journal of the Korean Society of Floral Art and Design
• /
• no.45
• /
• pp.13-29
• /
• 2021

Under the recognition that the technological system of traditional flower design in Korea has been passed down from the past to the present, we extracted the type of design form from the flower decoration of the Joseon Dynasty, analyzed the expression technique, and examined the relationship with the modern traditional flower decoration in this study. As a result, most of the flower design works of the Joseon Dynasty mainly expressed one focal point at the central starting point. One central branch line was mainly used, and cut leaves were mainly used as the base material. Similar to the Joseon Dynasty, one central starting point and a base made of leaves are mainly used In modern flower design. In the frequency of use by expression technique, the stem exposure technique was used the most, and cut flowers and cut stems were used at a similar frequency in all techniques. As plant materials, cut flowers and cut stems were mainly used. In addition, non-plant materials such as feathers, fans, and brushes were also used for decoration. In conclusion, it can be seen that traditional flower decorations in Korea were systematically established in the Joseon Dynasty in terms of expression techniques and high-level flower design works were made. In the future, it will be necessary to study the history of our flower designs in more diverse ways and to develop modern flower designs while preserving historical traditions.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.49 no.4
• /
• pp.365-373
• /
• 2023

Since the lips are a representative facial part that can express human attraction, aesthetic interest in them has always existed in human history. However, as lips age, they tend to form wrinkles, become thinner, and lose their volume. To counteract this phenomenon, medical procedures such as fillers or fat transplantation have been suggested. In this study, we verified that the one of main effective material complex of L G H&H L IPCERIN^®, combination of cedrol, a sesquiterpene found in cedarwood, and three peptides (acetyl hexapeptide-8, acetyl tetrapeptide-9, and desamidocollagen) could ameliorate the photo-aging and reduce the wrinkles through in vitro experiments. The possibility of improving collagen and elastin expression in skin fibroblasts and reducing MMP expression under photoaging conditions was verified. In addition, it was confirmed that the amount of fat tissue in the lips can be increased by promoting adipose stem cell differentiation and increasing the amount of fat produced in the in vitro adipose stem cell differentiation experiment. Two weeks of human application tests confirmed that a combination of cedrol and peptides can improve the wrinkles, texture, elasticity, and volume of the lips. This study verified that the combination of cedrol and three peptides can be used as effective cosmetic materials to decrease the various signs of aging in the lips.

• Journal of Korean Society of Forest Science
• /
• v.70 no.1
• /
• pp.28-37
• /
• 1985

In order to estimate breast-height form factors of Pinus thunbergii and Cryptomeria japonica, 8 models based on tree age, diameter at breast height and tree height were suggested and evaluated. It was the following equations that turned out to be most fit for estimating them; for Pinus thunbergii, $F=0.553-4.567\;1/A+71.409\;1/A^2$ ($R^2=0.928$), based on tree age, ($6.727^{**}$) ($14.100^{**}$) $F=0.356+1.774\;1/D-0.770\;1/D^2$ ($R^2=0.944$), based on diameter at breast height, ($15.102^{**}$) ($2.908^{**}$) $F=0.316+1.546\;1/H+0.397\;1/H^2$ ($R^2=0.941$), based on tree height, ($8.380^{**}$) ($3.896^{**}$) for Cryptomeria japonica, $F=0.400+2.348\;1/A+17.053\;1/A^2$ ($R^2=0.889$), based on tree age, ($3.501^{**}$) ($3.298^{**}$) $F=0.353+2.118\;1/D-1.462\;1/D^2$ ($R^2=0.923$), based on diameter at breast height, ($14.873^{**}$) ($3.545^{**}$) $F=0.403+0.427\;1/H+2.843\;1/H^2$ ($R^2=0.887$), based on tree height. ($3.254^{**}$) ($5.742^{**}$) The above estimated breast-height form factors proved to be overestimated for young trees and small diameter trees, and to be underestimated for old trees and large diameter trees, in comparison to generally accepted figure in Korea, that is, the form factor of 0.45.

• Journal of Plant Biotechnology
• /
• v.6 no.1
• /
• pp.15-19
• /
• 2004

The expression of a chimeric transgene (nos-npt II) has been examined in 9 years old transgenic poplars (Populus koreana x P. nigra) growing in a nursery. The expression of the gene in twenty six independentely transformed plants were examined by 1) enzyme (NPT II) assay, 2) RT-PCR, and 3) resistance to kanamycin. High NPT II activities in young leaves of all the transformed plants were found even without a selection pressure for antibiotics for 9 years. However, the activity varied with the positions of leaves in the stem in that young leaves showed higher activity than did mature tissues. When leaf segments were cultured in the presence of 150 mg/l kanamycin, only those from young leaves produced vigorously growing callus. However, as in the case of NPTII assay, the leaf segments from mature leaves did not form callus well on the media. RT-PCR with nptII specific primers also showed that amplification products were observed only when RNAs from young tissues were used. The total RNA gel showed that while RNA in young leaves are relatively stable and in a large quantity, those in old leaves were mostly degraded. All the above results suggest that the gene is transcriptionally active only in young tissue even though it is attached to a constituitive promoter. Therefore, the expression of foreign gene in poplar plants seemed to be affected by the metabolic state of the cells and thus vary greatly with the developmental stages and the age of tissue.