Lee, Chang Soo;Choi, Sang Hyun;Park, Jong Hyok;Kim, Young Ook
KSCE Journal of Civil and Environmental Engineering Research
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v.29
no.4A
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pp.373-384
/
2009
For the first step on the quantitative evaluation of shrinkage reduction and differential shrinkage analysis of lightweight aggregate concrete, this study sets the moisture transport model of concrete by pre-absorbed water of porous lightweight aggregates and measured effective moisture diffusion coefficient, moisture capacity, degree of humidity supply and degree of humidity consumption by water binder ratio and aggregate type. The effective moisture diffusion coefficient in steady state caused by humidity difference between inside and outside of concrete had low value as low water-binder ratio. And in case of same water-binder ratio, effective moisture diffusion of mixtures used normal aggregates were lower than those used lightweight aggregates. To determine moisture store capability of concrete - moisture capacity, moisture contents were measured in 9 humidity conditions. As a result moisture contents of mixtures used lightweight aggregates was higher than mixtures used normal aggregates in all humidity conditions. This study measured lightweight aggregates' degree of humidity supply that applicable to normal atmospheric environment (above RH 50%) and made it quantitatively. Also amount of moisture release was set as a exponential function that represents a clear trend proportion to time and inverse proportion to humidity of the surroundings. As the result of measurement about degree of moisture consumption inside concrete following the internal consumption caused by cement hydration self-drying, it was showed that rapid decrease of humidity, around 10%, at early ages (7~10 days) when water-binder ratio is 0.3 and slow decrease around 5% and 1% when water-binder ratio is 0.4 and 0.5.
A derivation of new release limit, named Derived Release Limit(DRL), into the atomsphere from a reference nuclear power plant has been performed on the basis of the new system of dose limitation recommended by the ICRP, instead of the (MPC)a limit which has been currently used until now as a general standard for radioactive effluents in Korea. In DRL Calculation, a Concentration Factor Method was applied, in which the concentrations of long-term routinely released radionuclides were in equilibrium with dose in environment under the steady state condition. The analytical model used in the exposure pathway analysis was the one which has been suggested by the USNRC and the exposure limits applied in this analysis were those recommended by the USEPA lately. In the exposure pathway analysis, all of the pathways are not considered and some may be excluded either because they are not applicable or their contribution to the exposure is insignificant compared with other pathways. In case, the environmental model developed in this study was applied to the Kori nuclear power plant as the reference power plant, the highest DRL value was calculated to be as $9.10{\times}10^6Ci/yr$ for Kr-85 in external whole body exposure from the semi-infinite radioactive cloud, while the lowest DRL value was observed 3.64Ci/yr for Co-60 in external whole body exposure from the contaminated ground, by the radioactive particulates. The most critical exposure pathway to an individual in the unrestricted area of interest (Kilchun-Ri, 1.3 km to the north of the release point) seems to be the exposure pathway from the contaminated ground and the most critical radionuclide in all pathways appears to be Co-60 in the same pathway. When comparing the actual release rate from KNU-l in 1982 with the DRL's obtained here the release of radionuclides from KNU-1 were much lower than the DRL's and it could be conclued that the exposure to an individual had been kept below the exposure limits recommended by the USEPA.
Slack-tide sampling was carried out at 6 stations at high and low tide for a tidal cycle during spring tide of the early summer (June) and summer (July, August) of 2016 to determine the difference of water quality according to tide in Masan Bay, Korea. The mixing regime of all the water quality components investigated was well explained through the correlation with SAL. In the early summer and summer, TURB, DSi and NNN which mainly flow into the bay from the streams and SS, COD, AMN and $H_2S$ which mainly indicate the internal sink and source materials have a property of conservative mixing and non-conservative mixing, respectively. The conservative mixing showed a good linear relationship of the water quality between high and low tide, and the non-conservative mixing showed a variation of different pattern each other. Factor analysis performed on the concentration difference data sets between high and low tide helped in identifying the principal latent variables for them. In early summer, multiple effects (tidal action, natural influx and internal sinks and sources etc.) acted in combination for the differences to be distributed evenly in four factors (VF1~4), since there were few allochthonous inputs as a low-water season. On the contrary, in summer, the parameters showing large concentration difference at ST-1 affected by stream water were concentrated in one factor (VF1) and clearly distinguished from the parameters affected by the internal sinks and sources. In fact, there is no estuary (bay) that always maintains steady state flow conditions. The mixing regime of an estuary might be changed at any time due to the change of flushing time, and furthermore the change of end-member conditions due to the internal sinks and sources makes the occurrence of concentration difference inevitable. Therefore, when investigating the water quality of the estuary, it is necessary to take a sampling method considering the tide to obtain average water quality data.
Difractose anhydrides (DFAs) is studied as a sweetener for diabetics because of its structural property. DFAs have four types: DFA I, III, IV (degradation of levan) and V (degradation of inulin). Especially, DFA IV has been shown to enhance the absorption of calcium in experiments using rats. Levan fructotransferase is an enzyme for producing di-d-fructose-2,6':6,2-dianhydride (DFA IV). To identify structural characterization, we purified wild-type and mutants (D63A, D195N and N85S) of levan fructotransferase (LFTase) from Microbacterium sp. AL-210. These proteins were purified to apparent homogeneity by Ni-NTA affinity column, Q-sepharose ion exchange and gel filtration chromatography and detected by SDS-PAGE. They were also analyzed by circular dichroism (CD) measurements, JNET secondary structure prediction, activity measurements at various temperatures, and pH analysis. The optimum pH for the enzyme-catalyzed reaction was pH 7.5 and optimum temperature was observed at $55^{\circ}C$. Along with wild-type LFTase, mutants were analyzed by CD measurement, fluorescence analysis and differential scanning calorimetry (DSC). N85S showed less $\alpha$-helix and more $\beta$ strand than others. Also, N85S showed almost the same curve as wild-type in their steady-state fluorescence spectra, whereas mutant D63A and D195N showed higher intensity than wild-type. The amino acid sequence of wild-type LFTase was compared to the sequences of exo-inulinase from Aspergillus awamori, a plant fructan 1-exohydrolase from Cichorium intybus, and Thermotogo maritime (Tm) invertase and showed a high identity with Exo-inulinase from Aspergillus awamori.
Hwang River in South Korea, has experienced channel adjustments due to dam construction. Hapcheon main dam and re-regulation dam. The reach below the re-regulation dam (45 km long) changed in flow regime, channel width, bed material distribution, vegetation expansion, and island formation after dam construction. The re-regulation dam dramatically reduced annual peak flow from 654.7 $m^3$/s to 126.3 $m^3$/s and trapped the annual 591 thousand $m^3$ of sediment load formerly delivered from the upper watershed since the completion of the dam in 1989. An analysis of a time series of aerial photographs taken in 1982, 1993, and 2004 showed that non-vegetated active channel width narrowed an average of 152 m (47% of 1982) and non-vegetated active channel area decreased an average of 6.6 km2 (44% of 1982) between 1982 and 2004, with most narrowing and decreasing occurring after dam construction. The effects of daily pulses of water from peak hydropower generation and sudden sluice gate operations are investigated downstream of Hapcheon Dam in South Korea. The study reach is 45 km long from the Hapcheon re-regulation Dam to the confluence with the Nakdong River. An analysis of a time series of aerial photographs taken in 1982, 1993, and 2004 showed that the non-vegetated active channel width narrowed an average of 152 m (47% reduction since 1982). The non-vegetated active channel area also decreased an average of 6.6 $km^2$ (44% reduction since 1982) between 1982 and 2004, with most changes occurring after dam construction. The average median bed material size increased from 1.07 mm in 1983 to 5.72 mm in 2003, and the bed slope of the reach decreased from 0.000943 in 1983 to 0.000847 in 2003. The riverbed vertical degradation is approximately 2.6 m for a distance of 20 km below the re-regulation dam. It is expected from the result of the unsteady sediment transport numerical model (GSTAR-1D) steady simulations that the thalweg elevation will reach a stable condition around 2020. The model also confirms the theoretical prediction that sediment transport rates from daily pulses and flood peaks are 21 % and 15 % higher than their respective averages.
Recently, transport parameters of conservative solutes such as KCl in a porous medium have been successfully determined using time domain reflectometry (TDR) . This study was initiated to Investigate the applicability of TDR technique to monitoring the fate of a heavy metal ion in a sandy soil and the distribution of its concentration along travel distance with time. A column test was conducted in a laboratory that consists of monitoring both resident and flux concentrations of $ZnCl_2$in a sandy soil under a breakthrough condition. A tracer of $ZnCl_2$(10 g/L) was injected onto the top surface of the sample as pulse type as soon as a steady-state condition was achieved. Time-series measurements of resistance and electrical conductivity were performed at 10 cm and 20 cm of distances from the inlet boundary by horizontal-positioning of parallel TDR metallic rods and using an EC-meter for the effluent exiting the bottom boundary respectively. In addition. Zn ions of the effluent were analyzed by ICP-AES. Since the mode and position of concentration detected by TDR and effluent were different, comparison between ICP analysis and TDR-detected concentration was made by predicting flux concentration using CDE model accommodating a decay constant with the transport parameters obtained from the resident concentrations. The experimental results showed that the resident concentration resulted in earlier and higher peak than the flux concentration obtained by EC-meter, implying the homogeneity of the packed sandy soil. A close agreement was found between the predicted from the transport parameters obtained by TDR and the measured $ZnCl_2$concentration. This indicates that TDR technique can also be applied to monitoring heavy metal concentrations in the soil once that a decay constant is obtained for a given soil.
Yea Sung Su;Jang Won Hee;Yang Young-Il;Lee Youn Jae;Kim Mi Seong;Seog Dae-Hyun;Park Yeong-Hong;Paik Kye-Hyung
Journal of Life Science
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v.15
no.1
s.68
/
pp.38-44
/
2005
Infection with hepatitis B virus (HBV) is a major health problem worldwide. Although a tremendous amount has been known about HBV, there have been obstacles in the study of HBV due to the narrow host range of HBV limited to humans and primates. In the present study, we investigated the susceptibility to HBV infection of mouse hepatoma cell line, Hepa-1c1c7. In addition, based on that human hepatocytes infected by HBV increase the expression of the pro-inflammatory cytokine TNF-a, the inducibility of TNF-a expression by HBV in the cells was determined. HBV surface antigen (HBsAg) secretion was measured by the microparticle enzyme immunoassay and steady state mRNA expression was analyzed by quantitative competitive RT-PCR. Transient transfection of Hepa-1c1c7 cells with HBV expression vector resulted in a dose-dependent induction of TNF-a expression. Infection of Hepa-1c1c7 cells with the serum of HBV carrier also increased TNF-a mRNA expression. Both in the transfected and infected cells, HBV mRNA was expressed and significant HBsAg secretion was detected. There was no significant variation in $\beta-actin$ mRNA expression by HBV. These results demonstrate that HBV is infectious to Hepa-lc1c7 in vitro and the viral infection induces TNF-a expression, which suggests that Hepa-lc1c7, a mouse hepatoma cell line, may be a possible model system for analysis of various molecular aspects of HBV infection.
Journal of the Korean Institute of Telematics and Electronics
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v.26
no.2
/
pp.94-101
/
1989
Transport properties of 2DEG at AlGaAs/GaAs interface such as average electron energy, flight distance, each valley occupancy ratio, average electron velocity for various fields are investigated by MONTE-CARLO method. As the electric field increases, more electrons transit drastically from (000) valley to (000) upper valley. This phenomenon shows the nonstationary effect such as velocity overshoot. The duration of the transient decreases from about 1.4 psec for electric field E = 7KV/cm to about 0.7 psec for 12KV/cm. The average electron velocity during transient transport in 2DEG is about 8 times the steady-state velocity for E = 12KV/cm at room temperature. In comparison with bulk GaAs the peak velocity in the 2DEG is higher than that in even pure bulk GaAs at electric field E = 7 KV/cm. On the basis of the fact that the electrons in the 2DEG have larger peak velocity and shorter transient time of velocity than those in the bulk GaAs, it is suggested that the device with 2DEG may obtain higher mobility than that with bulk GaAs.
Park Byung-Kwon;Lim Jong-Hwan;Kim Myoung-Seok;Hwang Youn-Hwan;Yun Hyo-In
Journal of Veterinary Clinics
/
v.23
no.2
/
pp.114-118
/
2006
A study on bioavailability and pharmacokinetics of florfenicol was conducted in broilers following a single intravenous (i.v.) and oral (p.o.) doses of 20 mg/kg body weight (b.w.). Florfenicol concentrations in plasma were determined by a high-performance liquid chromatography/mass spectrometry. Plasma concentration-time data after i.v. administration were analyzed by a non-compartmental analysis. Following i.v. injection, the total body clearance was $0.74{\pm}0.25L/kg/h$ and the volume of distribution at steady-state was $1.16{\pm}0.19L/kg$. Florfenicol was rapidly distributed and eliminated following i.v. injection with $1.15{\pm}1.06h$ of elimination half-life. After oral administration, the calculated $C_{max}$ values ($8.18{\pm}0.97{\mu}g/mL$) were reached at $1.33{\pm}0.29h$ in broilers. The elimination half-life of florfenicol was $1.31{\pm}0.27h$ and the absolute bioavailability (F) was 75.46% after oral administration of florfenicol. Florfenicol amine, a major metabolite of florfenicol, was detected in all broilers after i.v. and p.o. administration of florfenicol. The observed $C_{max}$ values of florfenicol amine ($3.96{\pm}2.60\;and\;2.22{\pm}1.71{\mu}g/mL$) were reached at $0.16{\pm}0.19\;and\;1.61{\pm}1.02h$ after i.v. and p.o. administration of florfenicol, respectively. Florfenicol amine was eliminated with $1.88{\pm}0.39\;and\;2.64{\pm}1.39h$ of the elimination half-life after i.v. and p.o. administration of florfenicol, respectively.
Stream inflows induced by flood runoffs have a higher density than the ambient reservoir water because of a lower water temperature and elevated suspended sediment(SS) concentration. As the propagation of density currents that formed by density difference between inflow and ambient water affects reservoir water quality and ecosystem, an understanding of reservoir density current is essential for an optimization of filed monitoring, analysis and forecast of SS and nutrient transport, and their proper management and control. This study was aimed to quantify the characteristics of inflow density current including plunge depth($d_p$) and distance($X_p$), separation depth($d_s$), interflow thickness($h_i$), arrival time to dam($t_a$), reduction ratio(${\beta}$) of SS contained stream inflow for different flood magnitude in Daecheong Reservoir with a validated two-dimensional(2D) numerical model. 10 different flood scenarios corresponding to inflow densimetric Froude number($Fr_i$) range from 0.920 to 9.205 were set up based on the hydrograph obtained from June 13 to July 3, 2004. A fully developed stratification condition was assumed as an initial water temperature profile. Higher $Fr_i$(inertia-to-buoyancy ratio) resulted in a greater $d_p,\;X_p,\;d_s,\;h_i$, and faster propagation of interflow, while the effect of reservoir geometry on these characteristics was significant. The Hebbert equation that estimates $d_p$ assuming steady-state flow condition with triangular cross section substantially over-estimated the $d_p$ because it does not consider the spatial variation of reservoir geometry and water surface changes during flood events. The ${\beta}$ values between inflow and dam sites were decreased as $Fr_i$ increased, but reversed after $Fr_i$>9.0 because of turbulent mixing effect. The results provides a practical and effective prediction measures for reservoir operators to first capture the behavior of turbidity inflow.
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