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http://dx.doi.org/10.5352/JLS.2005.15.1.038

Hepatitis B Virus-Induced TNF-a Expression in Hepa-lc1c7 Mouse Hepatoma Cell Line  

Yea Sung Su (Department of Biochemistry, College of Medicine, Inje University)
Jang Won Hee (Paik Institute for Clinical Research)
Yang Young-Il (Paik Institute for Clinical Research, Department of Pathology, College of Medicine, Inje University)
Lee Youn Jae (Department of Internal Medicine, College of Medicine, Inje University)
Kim Mi Seong (Paik Institute for Clinical Research)
Seog Dae-Hyun (Department of Biochemistry, College of Medicine, Inje University)
Park Yeong-Hong (Department of Biochemistry, College of Medicine, Inje University)
Paik Kye-Hyung (Paik Institute for Clinical Research)
Publication Information
Journal of Life Science / v.15, no.1, 2005 , pp. 38-44 More about this Journal
Abstract
Infection with hepatitis B virus (HBV) is a major health problem worldwide. Although a tremendous amount has been known about HBV, there have been obstacles in the study of HBV due to the narrow host range of HBV limited to humans and primates. In the present study, we investigated the susceptibility to HBV infection of mouse hepatoma cell line, Hepa-1c1c7. In addition, based on that human hepatocytes infected by HBV increase the expression of the pro-inflammatory cytokine TNF-a, the inducibility of TNF-a expression by HBV in the cells was determined. HBV surface antigen (HBsAg) secretion was measured by the microparticle enzyme immunoassay and steady state mRNA expression was analyzed by quantitative competitive RT-PCR. Transient transfection of Hepa-1c1c7 cells with HBV expression vector resulted in a dose-dependent induction of TNF-a expression. Infection of Hepa-1c1c7 cells with the serum of HBV carrier also increased TNF-a mRNA expression. Both in the transfected and infected cells, HBV mRNA was expressed and significant HBsAg secretion was detected. There was no significant variation in $\beta-actin$ mRNA expression by HBV. These results demonstrate that HBV is infectious to Hepa-lc1c7 in vitro and the viral infection induces TNF-a expression, which suggests that Hepa-lc1c7, a mouse hepatoma cell line, may be a possible model system for analysis of various molecular aspects of HBV infection.
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