• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.233-236
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• 2006

This study was conducted to investigate somatic embryogenesis capacity using callus derived from bud meristems in sweetpotato. Shoot apical meristem explants $(height:150{\mu}m;base:\;350{\mu}m)$were cultured on MS medium supplemented with 1 mg/L 2/4-D. Embryogenic callus were observed in five cultivars when their shoot apices were cultured on MS medium supplements with 1 mg/L 2,4-D. After 6 weeks of culture, greater than 80% of the survived explants produced embryogenic calli and the calli gave rise to somatic embryos at frequencies of 72% (Yulmi), 60% (Shinhwangmi), 78% (Geonmi), 70% (KoKei 14), 40% (Sinjami). The regenerated plants developed into whole plantlets after they were transferred onto the fresh hormon-free MS medium of 74% (Yulmi), 82% (Shinhwangmi), 86% (Geonmi), 74% (Kokei 14), 41% (Sinjami) respectively.

• Journal of Plant Biotechnology
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• v.29 no.3
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• pp.189-192
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• 2002

An efficient plant regeneration system of major cultivars of sweetpotato (Ipomoea batatas (L.) Lam.) in Korea via somatic embryogenesis was established. Embryogenic calli were formed from shoot apical meristems of sweetpotato cultivars when cultured on LS medium supplemented with 1 mg/L auxin (2,4-D, picloram, dicamba). Among three kinds of auxin, 1 mg/L 2,4-D showed the highest embryogenic calli induction rate. After 4 weeks of cultures on LS medium supplemented with 1 mg/L 2,4-D, embryogenic calli induction rates of Sinhwangmi, Zami, Yulmi, and White Star were 86%, 78%, 76%, and 80%, respectively. Upon transfer onto LS basal medium, most of somatic embryos developed into plantlets. Regenerated plantlets were transplanted to potting soil and grown to mature plants in a greenhouse.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.5
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• pp.375-379
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• 2001

Transgenic plants from hypocotyl segments of buckwheat were produced with the Agrobacterium strain LBA4404 harboring the binary vector pBI121 containing chimeric genes of neomycin phosphotransferase II (npt II) and $\beta$-glucuronidase (gus). Two weeks after co-cultivation with Agrobacterium, most of the hypocotyl segments gradually became brown and died on the selection medium containing 100mg/$\ell$ of kanamycin. Plants regenerated from the hypocotyl explants grown on selection medium were GUS-positive in the leaf, stem and vascular tissues by histochemical assay, and varied in gus activity (440-2568 pmol, 4-MU/mg protein) by fluorimetry. The plants showing GUS activity were confirmed of containing GUS and NPT-II genes by polymerase chain reaction (PCR). Within 3 months, transgenic buckwheat plants were able to obtained from the hypocotyl segments.

• Journal of the Korea Convergence Society
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• v.13 no.3
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• pp.163-172
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• 2022

The purpose of this study is to present implications in the planning and design methodology for revitalization of public spaces for mixed-use residential building. For the purpose, I have taken a literature research and a site analysis with cases of 'Kyungsung Univ.·Bukyung Nat'l Univ. Station'-area in Busan City. The analysis framework was consist of spatial composition, street facilities, use behavior, and connection with the street. The result of this study is as follows; 1) It is needed to consist of an empty space practically usable in an open location that is easy to access by the general public. 2) Considering the use of people, it is needed to install street furniture that can actually be used for rest. 3) It is needed to make a plan and design that considers the access and use of the general public and the space through it. 4) It is needed to create a synergistic effect in the integration of the street and the public space and the linkage with the street through this.

• Journal of Sericultural and Entomological Science
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• v.30 no.1
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• pp.1-7
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• 1988

Flower buds of the mulberry(Morus alba L., Morus bombycis Koidz.) were cultured under different conditions such as basal media, and various concentrations of plant growth substances. Effects of the culture conditions on growth of the buds and organ regeneration were investigated and the result obtained are as follows: Murashige and Skoog(M.S.) medium was more effective on budding and growth of female(Keomseolppong) and male(Kaeryangppong) flower buds isolated directly from branches, compared to Greshoff & Doy(G.D.) and Wolter & Skog(W.S.) media. The growth of the female buds was promoted at higher concetration of benzyl amino purine(BAP) i.e., 2.0ppm. The female and male buds cultured after cuuting for seven days showed better growth than those without cutting treatment. The females and the males bloomed to form healthy stigmas and anthers, respectively, when cultured on M.S. media containing high Kinetin with low concentration of indole acetic acid(IAA).

• Particle and aerosol research
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• v.5 no.2
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• pp.37-43
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• 2009

Particulate matter (PM) is one of the major indoor air pollutants especially in the subway station in Korea. In order to remove PM in the subway station, several kinds of PM removal system such as roll-filter, auto-washable air filter, demister, and electrostatic precipitator are used in the air handling unit (AHU) of subway stations. However, those systems are prone to operation and maintenance problems since the filter-regeneration unit consisting of electrical or water jet parts might malfunction due to the high load of particulates unless the filter medium is periodically replaced. In this study, the use of axial-flow cyclone was proposed for particulate filter unit in the AHU for its low operation and maintenance cost. Novel shape of axial-flow cyclone was designed by using computational fluid dynamics (CFD). The shape of vortex vane was optimized in terms of pressure drop and tangential velocity. In addition, CFD analysis was validated experimentally through the pressure drop measurement of mock-up model. We found that pressure drop and tangential velocity of fluid through the axia-flow cyclone was significantly affected by the rotating degree of vortex vane and the numerical prediction of pressure drop agreed well with experimental measurement.

• Journal of Radiation Industry
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• v.4 no.4
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• pp.373-379
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• 2010

Cellulose, chitin, chitosan and hyaluronic acid are well known as polysaccharides. These polysaccharides have many effects on cell growth and differentiation. Cell activation increases with increasing the polysaccharides concentration. In this study, gelatin scaffold containing microorganism fermented cellulose, citrus gel were prepared by using irradiation technique. Physical properties of the scaffolds were investigated as a function of the concentrations of gelatin and citrus gel and the cell attachment, cell morphology and inflammation of the scaffolds also were characterized for regeneration of skin tissue.

• Korean Journal of Medicinal Crop Science
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• v.2 no.3
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• pp.219-226
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• 1994

Immature ovule of intergeneric $F_1$ hybrid between Platycodon grandiflorum x Codonopsis lanceolata for producing embryogenic callus. somatic embryos and plant regeneration were cultured in vitro on various medium as well as MT(Murashige Tucker)medium treated with different concentration of plant growth regulators. Embryogenic callus induction was highest in the treatment of NAA 0.5 $mg/{\ell}$ and zeatin 0.01 $mg/{\ell}$ added on MT medium, whereas it was lower in treatments with auxins alone. MT medium were more effective in production of somatic embryos from incubated embryogenic callus. Most favorable plant growgh regulator for producing somatic embryos was 2. 4-D 0.5 $mg/{\ell}$ and zeation. BAP 0.01$mg/{\ell}$, but hormone-free and auxins alone were less effective. NAA 0.01$mg/{\ell}$ added with zeation 0.5 $mg/{\ell}$ was effective as high as NAA 0.01 $mg/{\ell}$ alone in normal plant regeneration from somatic embryo.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.12
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• pp.5165-5176
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• 2010

This study is an attempt to seek ways to revitalize the main streets in local city with regard to urban regeneration. It focuses on the case of Myeong-dong Fashion Street located in front of Cheonan Station, which recently lost its vitality. In order to explore causes of the decline and solutions for restore, this paper investigate the concerned urban area by approaching through three different aspects: physical and environmental aspects, social and economic aspects, historic and cultural aspects. In addition, the street visitors were surveyed on their visiting patterns, priorities and discontents about the components of street, and preferences for its restoring, etc. The investigation resulted in following findings and proposals. In physical and environmental dimension, it is needed to create legal incentives for encouraging local residents to develop their own district, and suggested to plan green space for relaxation and cultural arts space by using existing buildings, along with the expansion of public parking. In regard of social economic aspects, it is proposed to give each street specialized commercial theme. At the historic and cultural level, it is suggested to plan the pedestrian network which links the Fashion Street with surrounding historic elements.

• Journal of Korea Soil Environment Society
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• v.4 no.2
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• pp.11-31
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• 1999

Removal of hexavalent chromium from artificial groundwater (AGW) by granular activated carbon (GAC) was investigated in batch and continuous-flow column studies. Experimental parameters that were examined included solution pH, presence of dissolved oxygen (DO), and GAC pretreatment with Fe(II). As the solution pH increased from 4 to 7.5, the amount of Cr(VI) removed by both GACs decreased significantly. Exclusion of DO from the experimental systems resulted in greater removal of Cr(VI) from solution, possibly as a result of reduction to Cr(III). However, pretreatment of the GAC with a reductant (Fe(II)) did not improve Cr(VI) removal. Equilibration With 0.01 M $K_2$$HPO_4$[to extract adsorbed Cr(VI)] followed by a wash with 0.02 N $K_2$$HPO_4$[to remove precipitated/sorbed Cr(III)] proved to be a viable approach for the regeneration of carbons whose Cr(VI) removal capacities had been exhausted. The performance of the regenerated carbons exceeded that of the virgin carbons, primarily because of the favorable adsorption of Cr(VI) at lower pH values and the reduction of Cr(VI) to Cr(III), The presence of Cr(III) in acid wash solutions provides direct evidence that Cr(VI) is reduced to Cr(III) in GAC systems under relatively acidic conditions. GAC performance over five complete cycles was consistently high, which suggests that such a system will be able to function over many operation cycles without deleterious effects.