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http://dx.doi.org/10.5010/JPB.2002.29.3.189

Plant Regeneration of Major Cultivars of Sweetpotato (Ipomoea batatas) in Korea via Somatic Embryogenesis  

Kwon, Eun-Jeong (Laboratory of Plant Cell Biotechnology)
Kwon, Suk-Yoon (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kim, Moon-Za (Department of Life Science, Mokwon University)
Lee, Joon-Seol (Mokpo Experiment Station/RDA)
Ahn, Young-Sup (Mokpo Experiment Station/RDA)
Jeong, Byeong-Choon (Mokpo Experiment Station/RDA)
Kwak, Sang-Soo (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee, Haeng-Soon (Laboratory of Plant Cell Biotechnology)
Publication Information
Journal of Plant Biotechnology / v.29, no.3, 2002 , pp. 189-192 More about this Journal
Abstract
An efficient plant regeneration system of major cultivars of sweetpotato (Ipomoea batatas (L.) Lam.) in Korea via somatic embryogenesis was established. Embryogenic calli were formed from shoot apical meristems of sweetpotato cultivars when cultured on LS medium supplemented with 1 mg/L auxin (2,4-D, picloram, dicamba). Among three kinds of auxin, 1 mg/L 2,4-D showed the highest embryogenic calli induction rate. After 4 weeks of cultures on LS medium supplemented with 1 mg/L 2,4-D, embryogenic calli induction rates of Sinhwangmi, Zami, Yulmi, and White Star were 86%, 78%, 76%, and 80%, respectively. Upon transfer onto LS basal medium, most of somatic embryos developed into plantlets. Regenerated plantlets were transplanted to potting soil and grown to mature plants in a greenhouse.
Keywords
Embryogenic callus; shoot apical meristem; somatic embryo;
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Times Cited By KSCI : 2  (Citation Analysis)
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