• Title/Summary/Keyword: State preference

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Recombinant Expression and Characterization of Thermoanaerobacter tengcongensis Thermostable $\alpha$-Glucosidase with Regioselectivity for High-Yield Isomaltooligosaccharides Synthesis

  • Zhou, Cheng;Xue, Yanfen;Zhang, Yueling;Zeng, Yan;Ma, Yanhe
    • Journal of Microbiology and Biotechnology
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    • v.19 no.12
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    • pp.1547-1556
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    • 2009
  • A novel thermostable $\alpha$-glucosidase (TtGluA) from Thermoanaerobacter tengcongensis MB4 was successfully expressed in E. coli and characterized. The TtgluA gene contained 2,253 bp, which encodes 750 amino acids. The native TtGluA was a trimer with monomer molecular mass of 89 kDa shown by SDS-PAGE. The purified recombinant enzyme showed hydrolytic activity on maltooligosaccharides, p-nitrophenyl-$\alpha$-D-glucopyranide, and dextrin with an exotype cleavage manner. TtGluA showed preference for short-chain maltooligosaccharides and the highest specific activity for maltose of 3.26 units/mg. Maximal activity was observed at $60^{\circ}C$ and pH 5.5. The half-life was 2 h at $60^{\circ}C$. The enzyme showed good tolerance to urea and SDS but was inhibited by Tris. When maltose with the concentration over 50 mM was used as substrate, TtGluA was also capable of catalyzing transglycosylation to produce $\alpha$-1,4-linked maltotriose and $\alpha$-1,6-linked isomaltooligosaccharides. More importantly, TtGluA showed exclusive regiospecificity with high yield to produce $\alpha$-1,6-linked isomaltooligosaccharides when the reaction time extended to more than 10 h.

Heart rate variability of students in hospital clinical practice by MBTI character types (MBTI성격유형에 따른 병원 임상실습 중인 응급구조학과 학생의 심박변이도 변화에 관한 연구)

  • Jung, Jun-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.12
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    • pp.6373-6386
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    • 2013
  • This study examined the relationship between the MBTI character types and heart rate variability. The subjects were 25 students in the department of emergency medical technology in C city, Chungnam, Korea. The heart rate variability was measured under two conditions: a stable state, and in the second week and fourth week in clinical practice. The period of the heart rate variability measurements was from Jun. 21, 2013 to Jul. 26, 2013.The changes in the HRV at the stable state and during clinical practice depending on the MBTI character types, and the HRV at the stable state and during clinical practice according to the distribution chart of four character types showed a significant difference in the nHF and standard deviation of all R-R intervals (SDNN). In the changes in the HRV at the stable state and during clinical practice depending on the preference distribution chart of each indicator, the T-F group and J-P group in the nLF, T-F group and J-P group in the nHF, S-N group in the standard deviation of all R-R intervals (SDNN) and the J-P group in the mHR showed a significant difference.

Dietary Frequencies of Korean American Adolescents in California: Correlation of Acculturation and Parental Dietary Consumption

  • Song YoonJu;Paik Hee Young;Park Haeryun;Hofstetter C. Richard;Hovell Melbourne F.;Irvin Veronica;Lee Jooeun
    • Nutritional Sciences
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    • v.8 no.2
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    • pp.104-110
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    • 2005
  • The purpose of this study was to examine dietary frequencies among Korean American adolescents in California, including the correlation of acculturation and parental dietary Consumption. This study was drawn from cross-sectional telephone surveys based on data from a larger study of Korean Americans in California using random sampling of residential phone listings. A total of 494 Korean American adolescents, ages 12 through 17, in California were collected with dietary frequencies and acculturation and one parents' demographic and dietary consumption information. Three-quarters of adolescents were classified as 'more acculturated group' into the U.S. society with a lower preference to Korean foods, frequent snacking, and lower consumptions of rice and kimchi. Other quarter of adolescents was classified as 'less acculturated group'. However, the most frequent food items by both groups were rice and kimchi. Fruit and vegetable consumption was associated between adolescents and parents in both groups, but rice and kimchi were associated only among the more-acculturated group. In conclusion, dietary frequencies of Korean American adolescents were influenced by acculturation and parental dietary consumption. These findings should inform future epidemiological researches as well as efforts to enhance the diet of Koreans and other immigrants.

Optimal Weather Variables for Estimation of Leaf Wetness Duration Using an Empirical Method (결로시간 예측을 위한 경험모형의 최적 기상변수)

  • K. S. Kim;S. E. Taylor;M. L. Gleason;K. J. Koehler
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.4 no.1
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    • pp.23-28
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    • 2002
  • Sets of weather variables for estimation of LWD were evaluated using CART(Classification And Regression Tree) models. Input variables were sets of hourly observations of air temperature at 0.3-m and 1.5-m height, relative humidity(RH), and wind speed that were obtained from May to September in 1997, 1998, and 1999 at 15 weather stations in iowa, Illinois, and Nebraska, USA. A model that included air temperature at 0.3-m height, RH, and wind speed showed the lowest misidentification rate for wetness. The model estimated presence or absence of wetness more accurately (85.5%) than the CART/SLD model (84.7%) proposed by Gleason et al. (1994). This slight improvement, however, was insufficient to justify the use of our model, which requires additional measurements, in preference to the CART/SLD model. This study demonstrated that the use of measurements of temperature, humidity, and wind from automated stations was sufficient to make LWD estimations of reasonable accuracy when the CART/SLD model was used. Therefore, implementation of crop disease-warning systems may be facilitated by application of the CART/SLD model that inputs readily obtainable weather observations.

Monosaccharide as a Central Scaffold Toward the Construction of Salicylate-Based Bidentate PTP1B Inhibitors via Click Chemistry

  • Tang, Yan-Hui;Hu, Min;He, Xiao-Peng;Fahnbulleh, Sando;Li, Cui;Gao, Li-Xin;Sheng, Li;Tang, Yun;Li, Jia;Chen, Guo-Rong
    • Bulletin of the Korean Chemical Society
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    • v.32 no.3
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    • pp.1000-1006
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    • 2011
  • The discovery of carbohydrate-based bioactive compounds has recently received considerable interest in the drug development. This paper stresses on the application of 1-methoxy-O-glucoside as the central scaffold, whereas salicylic pharmacophores were introduced with diverse spatial orientations probing into the structural preference of an enzymatic target, i.e. protein tyrosine phosphatase 1B (PTP1B). By employing regioselective protection and deprotection strategy, 2,6-, 3,4-, 4,6- and 2,3-di-O-propynyl 1-methoxy-O-glucosides were previously synthesized and then coupled with azido salicylate via click chemistry in forming the desired bidentate salicylic glucosides with high yields. The inhibitory assay of the obtained triazolyl derivatives leads to the identification of the 2,3-disubstituted salicylic 1-methoxy-O-glucoside as the structurally privileged PTP1B inhibitor among this bidentate compound series with micromole-ranged $IC_{50}$ value and reasonable selectivity over other homologous PTPs tested. In addition, docking simulation was conducted to propose a plausible binding mode of this authorized inhibitor with PTP1B. This research might furnish new insight toward the construction of structurally different bioactive compounds based on the monosaccharide scaffold.

The Impact of Collective Guilt on the Preference for Japanese Products (집체범죄감대경향일본산품적영향(集体犯罪感对倾向日本产品的影响))

  • Maher, Amro A.;Singhapakdi, Anusorn;Park, Hyun-Soo;Auh, Sei-Gyoung
    • Journal of Global Scholars of Marketing Science
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    • v.20 no.2
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    • pp.135-148
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    • 2010
  • Arab boycotts of Danish products, Australian boycotts of French products and Chinese consumer aversion toward Japanese products are all examples of how adverse actions at the country level might impact consumers' behavior. The animosity literature has examined how consumers react to the adverse actions of other countries, and how such animosity impacts consumers' attitudes and preferences for products from the transgressing country. For example, Chinese consumers are less likely to buy Japanese products because of Japanese atrocities during World War II and the unjust economic dealings of the Japanese (Klein, Ettenson and Morris 1998). The marketing literature, however, has not examined how consumers react to adverse actions committed by their own country against other countries, and whether such actions affect their attitudes towards purchasing products that originated from the adversely affected country. The social psychology literature argues that consumers will experience a feeling called collective guilt, in response to such adverse actions. Collective guilt stems from the distress experienced by group members when they accept that their group is responsible for actions that have harmed another group (Branscombe, Slugoski, and Kappenn 2004). Examples include Americans feeling guilty about the atrocities committed by the U.S. military at Abu Ghraib prison (Iyer, Schamder and Lickel 2007), and the Dutch about their occupation of Indonesia in the past (Doosje et al. 1998). The primary aim of this study is to examine consumers' perceptions of adverse actions by members of one's own country against another country and whether such perceptions affected their attitudes towards products originating from the country transgressed against. More specifically, one objective of this study is to examine the perceptual antecedents of collective guilt, an emotional reaction to adverse actions performed by members of one's country against another country. Another objective is to examine the impact of collective guilt on consumers' perceptions of, and preference for, products originating from the country transgressed against by the consumers' own country. If collective guilt emerges as a significant predictor, companies originating from countries that have been transgressed against might be able to capitalize on such unfortunate events. This research utilizes the animosity model introduced by Klein, Ettenson and Morris (1998) and later expanded on by Klein (2002). Klein finds that U.S. consumers harbor animosity toward the Japanese. This animosity is experienced in response to events that occurred during World War II (i.e., the bombing of Pearl Harbor) and more recently the perceived economic threat from Japan. Thus this study argues that the events of Word War II (i.e., bombing of Hiroshima and Nagasaki) might lead U.S. consumers to experience collective guilt. A series of three hypotheses were introduced. The first hypothesis deals with the antecedents of collective guilt. Previous research argues that collective guilt is experienced when consumers perceive that the harm following a transgression is illegitimate and that the country from which the transgressors originate should be responsible for the adverse actions. (Wohl, Branscombe, and Klar 2006). Therefore the following hypothesis was offered: H1a. Higher levels of perceived illegitimacy for the harm committed will result in higher levels of collective guilt. H1b. Higher levels of responsibility will be positively associated with higher levels of collective guilt. The second and third hypotheses deal with the impact of collective guilt on the preferences for Japanese products. Klein (2002) found that higher levels of animosity toward Japan resulted in a lower preference for a Japanese product relative to a South Korean product but not a lower preference for a Japanese product relative to a U.S. product. These results therefore indicate that the experience of collective guilt will lead to a higher preference for a Japanese product if consumers are contemplating a choice that inv olves a decision to buy Japanese versus South Korean product but not if the choice involves a decision to buy a Japanese versus a U.S. product. H2. Collective guilt will be positively related to the preference for a Japanese product over a South Korean product, but will not be related to the preference for a Japanese product over a U.S. product. H3. Collective guilt will be positively related to the preference for a Japanese product over a South Korean product, holding constant product judgments and animosity. An experiment was conducted to test the hypotheses. The illegitimacy of the harm and responsibility were manipulated by exposing respondents to a description of adverse events occurring during World War II. Data were collected using an online consumer panel in the United States. Subjects were randomly assigned to either the low levels of responsibility and illegitimacy condition (n=259) or the high levels of responsibility and illigitemacy (n=268) condition. Latent Variable Structural Equation Modeling (LVSEM) was used to test the hypothesized relationships. The first hypothesis is supported as both the illegitimacy of the harm and responsibility assigned to the Americans for the harm committed against the Japanese during WWII have a positive impact on collective guilt. The second hypothesis is also supported as collective guilt is positively related to preference for a Japanese product over a South Korean product but is not related to preference for a Japanese product over a U.S. product. Finally there is support for the third hypothesis, since collective guilt is positively related to the preference for a Japanese product over a South Korean product while controlling for the effect of product judgments about Japanese products and animosity. The results of these studies lead to several conclusions. First, the illegitimacy of harm and responsibility can be manipulated and that they are antecedents of collective guilt. Second, collective guilt has an impact on a consumers' decision when they face a choice set that includes a product from the country that was the target of the adverse action and a product from another foreign country. This impact however disappears from a consumers' decision when they face a choice set that includes a product from the country that was the target of the adverse action and a domestic product. This result suggests that collective guilt might be a viable factor for company originating from the country transgressed against if its competitors are foreign but not if they are local.

A study on the efficient supporting methods for small and medium businesses of mobile sector (모바일 분야 중소기업에 대한 효율적 지원 방안 연구)

  • Ahn, Jong-Chang;Lee, Dong-Han;Jung, Gag-Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.2
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    • pp.192-201
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    • 2018
  • This study intends to find efficient support methods for small and medium-sized businesses (SMBs) especially in the increasingly important mobile sector. We investigated regarding perceptions of mobile sector-specific support programs and their current state of usage, and perceptions of the new mobile market and the present conditions of each stage of production based on analysis of a survey that included 204 respondents in each SMB to achieve this goal. Concretely, detections through this research were the reasons for non-selection of support businesses, the future prospective mobile business area, the company's own first-preference item and the reason for the preference, plus new mobile areas of willingness to apply the company's own product and its own first-preference item, and the reason for the preference. In addition, we found out covering costs and revenue size of the maximum-revenue product for each stage (from the first to the fourth stage), the kind of a product's execution method connected to the product (e.g. self-production, outsourcing to another company to produce a product), and the execution method for pre-products. We can suggest a policy regarding efficient support alternatives for SMBs related to the mobile sector thanks to these findings.

A Study on Consumer Awareness, Preference, and Consumption Behavior Regarding Local Food - Focusing on Gyeongju Area - (향토음식의 인지도와 기호도 및 소비행동에 관한 연구 - 경주지역을 중심으로 -)

  • Jang, Sun-Ok;Woo, Iee-Shik
    • Culinary science and hospitality research
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    • v.21 no.2
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    • pp.154-170
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    • 2015
  • In this study, the real state of local foods was examined targeting Gyeongju citizens to help in developing distinguishing foods and to promote cultural to excellence of local foods widely. Ultimately, this study aims to promote cultural excellence of Gyeongju's local foods widely by examining the kinds of local foods in Gyeonggju area. In addition, current study was conducted to provide basic data of local foods which inherit and develop our precious assets, in order to keep up with the Korean Wave as well as globalization. For this study, a survey was performed with the 274 sample of local food consumers, and analyzed the data using descriptive, cross tabulation analysis, and t-test. Results shown that Janchi guksu(banquet noodles) and Jeonbok juk(abalone rice porridge) obtained the highest awareness and preference when awareness and preference of Gyeongju local foods were analyzed depending on the marital status. Significant implication in the study is that there is a need to develop and promote local foods and open local food restaurants for local residents. More discussion and limitations are suggested.

Suggestions for the Activation of the Elementary Science Camp through the Present Status of Operation and Preference Survey (초등과학캠프의 운영 실태와 선호도 조사를 통한 활성화 방안)

  • Kwon, Nanjoo;Kim, Sangyong;Na, Sanghoon
    • Journal of Science Education
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    • v.34 no.2
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    • pp.306-319
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    • 2010
  • The purpose of this study is to pursue suggestions for the activation of the elementary science camp through the present status of operation and preference survey; examined 50 elementary science camps conducted since year 2004, and surveyed 590 people of students, parents and teachers around Seoul and Gyeonggi province. This study also explores ways of issues and solutions through interviews with five officials of the elementary science camp. This study drew data from the present status of the elementary science camp, the state of camp participation, student's preference, interviews with camp managers. The following are suggestions for the activation of the elementary science camp based on the results of this study; First, the elementary science camp especially in charge of science-related association or office of education should be enlarged, and other private institute or organization should prepare for specialized programs and expert teachers. Second, insufficient camp spot should be solved and participation fee differentials should be alleviated. Third, the period of the camp and its announcement should be various, so that students have more opportunities. In conclusion, managing elementary science camp needs specialism, and continuing effort, because it is related to many important matters such as theme, period, place, participation expenses, guiding teacher, number of student, program organization, student's preference, and method of notice. Therefore, this study insists steady cooperation, continuing endeavor, and appropriate support system for elementary science camps on a nationwide scale.

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The Relationship between Health and Mind-Body State According to Five Colors Preference (오색(五色)선호도에따른 건강과 심신상태의 관련성 연구)

  • Ahn, Ji-Hye
    • Journal of Naturopathy
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    • v.9 no.1
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    • pp.1-12
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    • 2020
  • Purposes: The purpose of this study was to investigate the relationship between five viscera, environmental and seasonal changes, and their preference for five colors. Methods: The survey performed using the Chinese medicine constitution and the five cardinal colors (Red, Blue, Yellow, White, Black) paper. Results: The blood types distributions were A, O, B, AB, and others in order. The proportion of smokers(75.6%) and non-drinkers(25.6%). Their color preferences were highest in blue and the lowest in black, but not significant. The proportion of the viscera was stomach(45.3%), liver-lung, intestine, and heart, and the tastes were spicy(48.8%), sweet, sour, salty, and bitter in order. Blue was the highest in all. kidneys(8.3%) preferred red and bitter yellow. The change in mind(35.7%) was highest, followed by scary thoughts and their color preferences varied. The most preferred place was 'cold place,' and 'dry place' was low. The most preferred seasons were 'autumn (38.6%), and the lowest was rainy. And their colors preference were blue. The respondents(89.9%) were normal, 5.7% hypertension and 4.4% hypotension. Healthy people preferred blue (91.8%) and hypertensives yellow with not significant. The diabetics preferred blue and red(p<.04). Conclusions: In the questionnaire survey on the health and physical abnormalities of Seoul citizens, various distributions found in each category, and most of them preferred blue. The results indicated that the questionnaire and the five- colored preference were a little interrelated. However, the results are to be primary data for research in this field.