• Korean Journal of Ichthyology
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• v.4 no.1
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• pp.20-28
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• 1992

The influence of starvation on morphological change and survival rate of the left eye flounder larvae was examined at the KORDI laboratories in March, 1990. 1. The larvae of left eye flounder began of feed on rotifers in 5 days after hatching. In case of non-feeding, all of the larvae died in 11 days after hatching. The larvae which fed 1 day after the normal first feeding schedule grew normally but 100 of the larvae died in 14 days when the feeding was delayed for 2 days after hatching. 2. With the exhaustion of the yolk, the total length, body length, myotome height and gut height of unfed larvae decreased. Gut height is the most decreased demensions while starving. 3. The ratio of gut height to myotome height in unfed larvae has declined most rapidly compare to other demensions during the starvation. At 13 days after hatching, the ratios of these between fed and unfed larvae were 0.797 and 0.467, respectively. 4. The morphology of starving larvae were characterized as sharpened jaw, projected edge of lawer part of clavicle and slender gut.

• International Journal of Industrial Entomology and Biomaterials
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• v.37 no.2
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• pp.100-104
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• 2018

The rearing of black soldier flies in Korea is affected by seasonal factors such as temperature and sun light; for this reason, it requires a great deal of effort to rear and maintain black soldier flies during the winter. In this study, we investigated how starvation affects larval development. After hatching the larvae, they were fed for 5 or 10 d and then starved for a certain period to see how they recovered. The length and width of larvae were estimated to be 18.18 to 21.96 mm, 5.19 to 6.04 mm, respectively. Larvae survivorship to the pupal stage was significantly different between groups and periods of starvation. The groups of fed for 5 d then starved showed a high survival rate until 20 d starvation and then the survival of larvae rapidly decreased. The survival rate of the larvae was abruptly decreased for 20 d starvation in the groups of fed for 10 d and starved, and then gradually decreased until 60 d starvation thereafter. Our research attempted to influence larvae development through starvation and provides basic information on how to culture the black soldier fly effectively and economically throughout the year.

• Korean journal of applied entomology
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• v.46 no.2
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• pp.295-302
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• 2007

We investigated starvation of hatching larvae, occasional artificial hatching and incubation method to establish year-round rearing of the wild silkmoth, Antheraea yamamai. In the test of starvation of hatching larvae for brushing at a time, the survival rate of the fourth instar of larvae starved for 1 day after hatching in $25^{\circ}C\;and\;5^{\circ}C$ was 83.3% and 96.0%, respectively. The result represents that the survival rate is high at low temperature during starvation. In the occasional artificial hatching test for multi-times rearing of A. yamamai, the useful hatchability is high at $5^{\circ}C$ in case of preserving eggs for 2 months from incubation time, and at both $2.5^{\circ}C\;and\;0^{\circ}C$ in case of over 6 months. A new incubation method with pre-incubation at $15^{\circ}C$ and 24 D photoperiod showed high hatchability about 80% for only 2 days compared with hatching for 5-6 days in traditional incubation method with the preservation at $25^{\circ}C$.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.76-77
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• 2003

Olive flounder Paralichthys olivaceus larvae and early juveniles were reared for 43 days after hatching in order to observe the effects of starvation during development and metamorphosis. Morphological, histological and biochemical measurements were made to assess the nutritional condition during growth and starvation from pre-matamorphic through post-metamorphic phases. Two groups of fish were compared ; one with sufficient food supply and one under continuous starvation until death. Among morphometric analyses, both ratios of body height at anus/head height and pre-/post-anal lengths appeared to be sensitive to starvation during which substantial reduction was observed within a day of food deprivation. Histological variables as intestinal and rectal epithelial heights and gall bladder volume changed significantly with onset of starvation. The gut epithelial heights of starving fish decreased with advances in starvation, although they fluctuated during mid-metamorphic phase. In contrast, gall bladder volume increased remarkably soon after starvation. Ontogenetic changes in both gut epithelial height and gall bladder volume were evident, those associated with settlement and/or completion of metamorphosis. Abrupt decrease in the RNA/DNA ratios of starving fish were found right after onset of starvation. Even in the fed fish marked fluctuations in its ratios during metamorphosis were observed, evident by decreasing from late-metamorphic to post-metamorphic stages. These findings suggest that a combination of morphologically and histologically sensitive characteristics, and biochemical measurement could be utilized as a measure to evaluate nutritional condition related to starvation in wild olive flounder larvae and juveniles.

• Journal of Aquaculture
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• v.11 no.4
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• pp.521-528
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• 1998

The influence of starvation on survival, growth and feeding incidence of larval tiger puffer Takifugu rubripes was investigated for 16 days after let them hatched at the temperature of 16.5~18.5${\circ}C$. The effect by delayed initial feeding on larvace was also studied. Almost all larvae began to be fed from the stage of yolk absorption about 5 days after hatching, in which rearing trials revealed that their survival and growth were influenced by food availability. Survival of unfed larvae, which stated to decrease drastically since the oil globule was exhausted about 9 days after hatching, dropped to 50% and 0% respectively on the 11.5th and 16th day. the difference of growth between fed and unfed larvae came into sight on about 5th day after hatching when the initial feeding was started, and had reduced in proportion to the length of starvation period. The condition factors for the unfed larvae were lower than thost of the fed. Of the larvae which had starved less than 5 days, more than 80% could consume food upon feeding. No fish had the ability in having food after 10 days. It appeared that the turning point of recover for the starving larvae came on 7th or 8th day. Their taking-food ability seemed to be affected by the change in swimming activity which depended on the duration of starvation. For the seed production of tiger puffer, therefore, feeding should be initiated as soon as possible, so as to enhance the production and survival of healthy seeds.

• Journal of Aquaculture
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• v.11 no.1
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• pp.11-17
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• 1998

Variations of hepatocyte in the larval liver of grouper, Epinephelus akaara wre examined to understand the effect of starvation during the first feeding period, 3 to 5 days after hatching. Total length of the fed larvae increased from the 5th day after hatching, although no significant difference between the fed and starved larvae was found untill the 4th day after hatching. Survival rate of the starved larvae decreased from the 4th day after hatching, and almost all of the larvae died by the 5th day after hatching. Nuclear size of hepatocyte in the starved larvae starterd to decrease from the 4th day after hatching. The sizes by 4th and 5th days after hatching in the starved larvae were 1.4 to 1.9 times smaller than those in the fed ones. Hepatocytes in the starved larvae showed irregular morphology in which the nuclei were irregularly shrunk and highly compacted from the 4th day, while hepatocyte nuclei in the fed ones maintained their uniform features during the whole experimental period. These results implied that the initial larval food should be supplied at least within the 4th day after hatching. Also, it suggested that the size of hepatocyte nucleus might be and indicator of starvation for wild and cultured grouper larvae.

• Korean Journal of Ichthyology
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• v.9 no.1
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• pp.15-21
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• 1997

The influence of delayed(1, 2, 3, 4 days) feeding and starvation on morphological change and survival rate of the spotted sea bass larvae was examined at the KORDI laboratories which located at Poryong Power Plant, Poryong-gun, Chungchongnam-do in November, 1996. 1. The larvae of spotted sea bass began to feed on rotifers at 5 days after hatching. In case of non-feeding, all of the larvae died at 9 days after hatching. The larvae which fed 1 day after the normal first feeding schedule(1 day delayed) grew normally and 2 days delayed groups showed 5.3% in survival rate at 9 days after hatching. In case of non-feeding and 3 or 4 days delayed groups, all of the larvae died between 9 and 10 days after hatching. 2. In case of non-feeding, total length of the larvae decreased gradually. 3. The percente ratio of gut height and mytome height to standard length in starved larvae has declined most rapidly compare to other demensions during the non-feeding period. The percente ratio of gut height to mytome height had also difference between unfed and fed larvae. At 9 days after hatching, the ratio of that between fed and unfed larvae were 84.5 % and 52.4 %, respectively. 4. The morphology of starving larvae were characterized as sharpened jaw, projected edge of lower part of clavicle and bending trunk with slenger gut.

• Korean Journal of Ichthyology
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• v.2 no.2
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• pp.138-148
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• 1990

The influence of starvation on morphological change of the red sea bream larvae was examined at Song-ji fish hatchery, Tongyong-Gun, Kyongnam Provice in July 1988. The results obtained are as follows: 1) The larvae of red sea bream began to feed on rotifers in 2 days after hatching. In case of non-feeding, all of the larvae died in 5 days after hatching and the larvae which feeding delayed 1 and 2 days from normal first feeding schedule also died 100 in 6 days after hatching. 2) With the exhaustion of the yolk, the total length, body length, myotome height and gut height of unfed larvae decreased. 3) The ratio of height to myotome height in unfed larvae has declined most rapidly compare to other demensions while starving. At 5 days after hatching, the ratios of these of starving larvae and fed larvae were 0.306 and 0.010, respectively. 4) The morphology of starving larvae at 6 days after hatching are characterized as sharpened jaw, projected edge of lower part of clavicle and slender gut.

• Development and Reproduction
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• v.17 no.4
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• pp.399-407
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• 2013

Morphological changes in the reared rock bream, Oplegnathus fasciatus, from hatching to six days after hatching were examined during the early growth stage under starvation. All the larvae died within five days when feeding was delayed for three days after hatching. These results imply that initial larval food should be supplied within two days of hatching. Changes in the pectoral angle and the ratios of eye height to head height, gut height to standard length, and gut height to myotome height in the rock bream are alternative indicators for the identification of starving fish. These indicators might prove useful in evaluating the successful transition from endogenous to exogenous feeding in this species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.5
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• pp.669-673
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• 1999

The effect of delayed initial feeding (1, 3, 5, 7 days) and starvation on morphological change, mortality and cannibalism on larvae of Siniperca scherzeri was examined by laboratory rearing. The larvae of S. scherzeri began to feed on Artemia nauplii at 4 days after hatching. In case of unfed and 7-days delayed groups, all of the larvae died at 12 days after hatching. The larvae of 1 day delayed feeding survived and grew as almost same as the control group, and 3-days delayed groups showed $33\%$ survival rate at the end of experiment (12 days after hatching). In case of the unfed group, total length of the starved larvae showed lower growth rate than the control group, and they did not reached at the same size of the larvae of the control group. Cannibalism were more common in the unfed group and the delayed fed group than the control group. The highest rate of daily mortality caused by cannibalism in the delayed fed group was $23\%$ at 8 days after hatching.