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Improvement of Occasional Artificial Hatching and Incubation Method in Diapause Egg of the Wild Silkmoth, Antheraea yamamai

천잠알의 수시부화 및 최청법 개선

  • Yoon, Hyung-Joo (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology) ;
  • Kang, Pil-Don (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology) ;
  • Lee, Sang-Mong (Department of Life Science and Environmental Biochemistry, College of Natural Resources and Life Science, Busan National University) ;
  • Kim, Sam-Eun (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology) ;
  • Kim, Ki-Young (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology)
  • 윤형주 (농촌진흥청 농업과학기술원 농업생물부) ;
  • 강필돈 (농촌진흥청 농업과학기술원 농업생물부) ;
  • 이상몽 (부산대학교 생명자원과학대학) ;
  • 김삼은 (농촌진흥청 농업과학기술원 농업생물부) ;
  • 김기영 (농촌진흥청 농업과학기술원 농업생물부)
  • Published : 2007.08.30

Abstract

We investigated starvation of hatching larvae, occasional artificial hatching and incubation method to establish year-round rearing of the wild silkmoth, Antheraea yamamai. In the test of starvation of hatching larvae for brushing at a time, the survival rate of the fourth instar of larvae starved for 1 day after hatching in $25^{\circ}C\;and\;5^{\circ}C$ was 83.3% and 96.0%, respectively. The result represents that the survival rate is high at low temperature during starvation. In the occasional artificial hatching test for multi-times rearing of A. yamamai, the useful hatchability is high at $5^{\circ}C$ in case of preserving eggs for 2 months from incubation time, and at both $2.5^{\circ}C\;and\;0^{\circ}C$ in case of over 6 months. A new incubation method with pre-incubation at $15^{\circ}C$ and 24 D photoperiod showed high hatchability about 80% for only 2 days compared with hatching for 5-6 days in traditional incubation method with the preservation at $25^{\circ}C$.

천잠의 연중 실내사육법을 확립하고자 천잠의 의잠절식, 수시부화 및 최청법을 조사하였다. 천잠의 일시소잠을 위하여 의잠을 절식 실험한 결과, $25^{\circ}C$$5^{\circ}C$ 에서 1일간 보호한 4령기잠기 생존율이 각각 83.3% 및 96.0% 로 의잠 절식기간의 보호온도가 낮을수록 생존율이 높았다. 연중 다회사육을 위해 수시부화실험을 한 결과, 천잠알을 2개월 냉장할 경우에는 $5^{\circ}C$가 좋았으나 6개월 이상 냉장하여 사용할 경우에는 $2.5^{\circ}C$또는 $0^{\circ}C$에 보호하는 것이 실용부화비율이 높은 것으로 나타났다. 또한 일시부화에 적합한 천잠알의 보호조건을 조사한 결과, 관행적 최청방법 ($25^{\circ}C$ 보호)으로는 5-6일 걸리는 천잠알의 부화가 $15^{\circ}C$ 예비최청과 암최청 방법의 도입으로 2일간에 80% 가 부화하는 좋은 성적을 얻을 수 있었다.

Keywords

References

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