• Journal of Microbiology and Biotechnology
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• 제6권2호
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• pp.145-146
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• 1996

The 2.4-kb cryptic plasmid (pKH8) of multidrug-resistant Staphylococcus aureus SA2 was characterized by complete nucleotide sequencing and homology comparison. pKH8 was found to contain three open reading frames. Protein analysis of pKH8 showed that pKH8 was a multidrug resistance plasmid and mediated resistance to ethidium bromide and quaternary ammonium compounds.

• Journal of the Korean Wood Science and Technology
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• 제30권3호
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• pp.26-33
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• 2002

The seven strains, Pseudomonas paucimobilis, Pseudomonas cepacia, Staphylococcus auricularis, Staphylococcus saprophyticus, Acidovorax spp., Acinetobacter calcoaceticus, and Actinobacillus capsulatus were tested with three slimicides. Most of the tested bacteria were inhibited with slimicide K (an isothiazolin based compound), even at its low concentration, except for Actinobacillus capsulatus and Staphylococcus auricularis. Both slimicides B (an organic bromine based compound) and S (aldehydes) also couldn't prevent these two strains even at their highest concentration. Five different sizes of plasmid DNA were isolated from Actinobacillus capsulatus. Staphylococcus auricularis, a gram-positive bacteria, showed the slimy substances around its cell distinctively. The results suggest that two strains, Actinobacillus capsulatus, Staphylococcus auricularis, have presumably developed a resistance to the slimicide, by plasmid DNA or slimy substance. Our findings also suggest that not only gram-negative bacteria, but also gram-positive bacteria should not be neglected

• 생명과학회지
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• 제4권2호
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• pp.60-63
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• 1994

Protein A는 Jensen법에 의하여 Staphylococcus aureus Cowan type 1 (SCI)주의 가열균 체에서 추출한 단백으로서 최근에는 lysostaphin-chromatography 방법으로 순수분리할 수 있는 natural protein이다. Protein A를 다량 함유한 SCI은 혈청중의 IgG를 흡착하므로 혈청 중에 IgG를 제거할 목적으로 사용할 수 있다. 본고에서는 protein A가 면양적혈구로 면역한 가토혈청의 적혈구 응집소가 및 용혈소가에 미치는 영향을 기술하였다.

• Journal of Korean Biological Nursing Science
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• 제10권2호
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• pp.147-153
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• 2008

Purpose: Methicillin Resistant Staphylococcus aureus (MRSA) has become a major clinical problem and one of the major nosocomial pathogen worldwide. The aim of the study was to investigate the epidemiological characteristics of genotypes of MRSA isolated in the A-hospital ICU. Methods: In the period between December 2007 and May 2008, MRSA was isolated from ICU patients and its surrounding environment. Polymerase Chain Reaction (PCR) was conducted for the detection of MRSA gene. The incidence of MRSA in the clinical isolates of Staphylococcus aureus was examined by using a multiplex PCR. The spa gene of Staphylococcus aureus encodes protein A and is used for typing of MRSA. We used sequence typing of the spa gene repeat region to study the epidemiology of MRSA at a hospital. Results: Two different genotypes of MRSA were identified with 90 isolated from the patients and its surrounding environments in the ICU. Conclusion: This study may contribute to the development of effective strategies for preventing nosocomial infections. Genotyping may have more general application for the study of MRSA epidemic outbreak in hospital and community infection.

• 미생물학회지
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• 제31권1호
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• pp.44-47
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• 1993

A replication initiation gene was identified and its nucleotide sequence has been determined from a 3.8 kb, chloramphenicol acethyltransferase conferring R-plasmid pSBK203 of Staphylococcus aures. Location of the replication related region of pSBK 203 was determined by interuption with pUC 119 at XBaI and MspI sites which resulted in inactivation of replication in Bacilius subtilis. Base sequence of this region revealed on open reading frame of 942 base pairs, which encoded a 314 amino acid protein. Base sequence homology with other rep of pT181 family plasmids such as pT181, pC221, pC223, pS194, pU112, and pCW7 was ranged from 78% to 97% and the predicted amino acid sequence homology was from 72% to 95%.

• Bulletin of the Korean Chemical Society
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• 제32권8호
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• pp.2695-2699
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• 2011

${\beta}$ Ketoacyl acyl carrier protein synthase III (KAS III) initiates fatty acid synthesis in bacteria and is a key target enzyme to overcome the antibiotic resistance problem. In our previous study, we found flavonoid inhibitors of Enterococcus faecalis KAS III and proposed three potent antimicrobial flavonoids against Enterococcus faecalis and Vancomycin-resistant Enterococcus faecalis with MIC values in the range of 128-512 ${\mu}g/mL$ as well as high binding affinities on the order from $10^6$ to $10^7\;M^{-1}$. Using these series of flavonoids, we conducted biological assays as well as docking study to find potent flavonoids inhibitors of Staphylococcus aureus KAS III with specificities against Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus. Here, we propose that naringenin (5,7,4'-trihydroxyflavanone) and eriodictyol (5,7,3',4'-tetrahydroxyflavanone) are potent antimicrobial inhibitors of Staphylococcus aureus KAS III with binding affinity of $3.35{\times}10^5$ and $2.01{\times}10^5\;M^{-1}$, respectively. Since Arg38 in efKAS III is replaced with Met36 in saKAS III, this key difference caused one hydrogen bond missing in saKAS III compared with efKAS III, resulting in slight discrepancy in their binding interactions as well as decrease in binding affinities. 4'-OH and 7-OH of these flavonoids participated in hydrogen bonding interactions with backbone carbonyl of Phe298 and Ser152, respectively. In particular, these flavonoids display potent antimicrobial activities against various MRSA strains in the range of 64 to 128 ${\mu}M$ with good binding affinities.

• 대한수의학회지
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• 제35권3호
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• pp.497-504
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• 1995

Isolation of Staphylococcus hyicus subsp. hyicus from the skin of healthy chickens was performed and biochemical characteristics of the isolates were examined. Staphylococcus hyicus subsp hyicus was isolated from 129(14.8%) of 874 healthy chickens. The rate of isolation of this organism from chickens was found to vary depending upon the poultry farms at the isolation rate of 0% to 38.7% and this organism was isolated more frequently from adult chickens of over 1 year old than young chickens. All of the isolates were negative for haemolysis on rabbit, sheep, pig and chicken blood agars, protein A and ${\beta}$-glucuronidase, but positive for coagulase using pig plasma, heat-stable DNase, phosphatase and Tween 80 hydrolysis. Hyaluronidase was positive in 97.7% of isolates, but their reaction was weaker than that of swine strains of Staphylococcus hyicus subsp hyicus. The isolates were biotyped on the basis of the reactions for protease, urease, nitrate, galactose, trehalose and lactose, and 11 biotypes were found among the 129 isolates of Staphylococcus hyicus subsp hyicus.

• 한국미생물·생명공학회지
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• 제40권3호
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• pp.274-277
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• 2012

본 연구에서는 다제내성을 보이는 인체 병원균의 하나인 S. aureus에서 유래된 FtsZ 단백질의 유전자를 클로닝하고 대장균에 형질전환하여 재조합 단백질을 만들고, in vitro 상에서 폴리머 형성 활성을 측정하였다. Bradford 방법을 이용하여 SA FtsZ단백질의 농도를 측정한 후, SA FtsZ단백질의 폴리머 형성 활성을 확인하기 위해 형광계를 이용하여 excitation 방향과 $90^{\circ}$의 방향에서 산란되는 빛의 양을 측정하는 방법을 사용하였을 때에 대조군에서는 빛이 산란되지 않았고, SA FtsZ 단백질에 GTP와 $Mg^{2+}$를 처리한 실험군에서만 빛이 산란되는 현상을 관찰하였다. 1분여의 시간이 지난 이후에는 다시 산란되는 빛이 줄어드는 것을 볼 수 있는데, 이것은 SA FtsZ 단백질의 아미노말단 도메인의 GTPase 활성에 의해서 GTP가 분해되어서 SA FtsZ 단백질의 폴리머가 단량체로 분해되었기 때문이라고 예측된다. 본 연구를 통하여 확립된 SA FtsZ 활성 측정 방법은 향후 SA FtsZ 단백질의 폴리머 형성을 저해하는 방식으로 S. aureus를 표적으로 하는 항생제 후보물질 도출을 위한 스크리닝 방법으로 사용될 수 있을 것이다.

• Archives of Pharmacal Research
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• 제22권1호
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• pp.25-29
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• 1999

Psammaplin A, a natural bromotyrosine derivative from an associated form of two sponges (Poecillastra sp. and jaspis sp.) was found to possess the antimicrobial effect on the Gram-positive bacteria, especially on methicillin-resistant Staphylococcus aureus (MRSA). The minimal inhibitory concentration of psammaplin A against twenty one MRSAs ranged from 0.781 to 6.25 ${\mu}g/ml$, which that of ciprofloxacin was 0.391~3.125${\mu}g/ml$. Psammaplin A could not bind to penicillin binding protein, but inhibited the DNA synthesis and the DNA gyrase activity with the respective 50% (DNA synthesis) and 100% (DNA gyrase) inhibitory concentration 2.83 and 100 ${\mu}g/ml$. These results indicate that psammaplin A has a considerable antibacterial activity, although restricted to a somewhat narrow range of bacteria, probably by inhibiting DNA gyrase.

• Clinical and Experimental Reproductive Medicine
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• 제47권3호
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• pp.186-193
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• 2020

Objective: The effectiveness of Staphylococcus protein A (SPA) in improving the penetration ability of sperm and reducing antisperm antibody (ASA) titers in immunologically infertile males was evaluated. Methods: Seminal fluid samples were obtained from 15 infertile men, and ASA titers were assessed with the latex agglutination test. Identification of immunoglobulin (Ig) classes and characterization of the antigens involved in the immune response were performed using indirect immunofluorescence. Local ASAs typically present as a mixture of IgG and IgA classes. The capillary tube penetration method was used to assess the capability of spermatozoa to penetrate the cervical mucus (CM). Results: ASAs associated with the neck region of sperm showed a significantly lower migration distance in the CM of infertile females than ASAs associated with the head or tail segments. ASA-positive seminal fluid exhibited significant increases in the mean migration distance (2.6 ± 1.4 cm vs. 1.54 ± 1.1 cm, respectively; p< 0.001) and sperm concentration (174 ± 121.0 × 10³/mL vs. 101 ± 93.7 × 10³/mL, respectively; p= 0.033) after treatment with SPA compared to pre-treated samples. A significant reduction (p< 0.01) in the recorded ASA titer was detected. Conclusion: These results indicate that SPA can be used as a sorting regimen for insemination programs. However, further studies are warranted to assess its influence on pregnancy rate.