• Asian-Australasian Journal of Animal Sciences
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• 제22권8호
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• pp.1209-1223
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• 2009

In this review, the terminology that is used to describe ileal amino acid (AA) digestibilities in piglet feed ingredients is defined. If one accepts that the determination of AA digestibilities should be based on the ileal analysis method, one should consider that ileal digesta contains variable amounts of endogenous crude protein (CP), which originates mainly from digestive secretions, sloughedoff epithelial cells and mucins. The ileal endogenous CP and AA losses are separated into basal ileal endogenous CP and AA losses ($IAAL_{B}$), which are not influenced by the feed ingredient composition, and specific ileal endogenous CP and AA losses ($IAAL_{S}$), which are induced by feed ingredient characteristics such as level and type of fiber and anti-nutritional factors (ANF). Depending how ileal endogenous CP and AA losses are considered in the measurement of CP and AA digestibilities, digestibility values are expressed as apparent (AID), standardized (SID), or true (TID) ileal digestibilities of CP and AA. The main concern associated with the use of AID values in diet formulation for pigs is that they are not additive in mixtures of feed ingredients. Consequently, the concept of standardized ileal CP and AA digestibilities was introduced by correcting AID values for basal ileal endogenous CP and AA losses ($IAAL_{B}$). The correction for both $IAAL_{B}$ and $IAAL_{S}$ yields TID values, however, routine procedures to measure $IAAL_{S}$ are not yet available. In principle, SID values should be preferred, because they represent the fundamental properties of the feed ingredient. There exist only few reports on SID of CP and AA in feedstuffs frequently used in piglet nutrition. These include soybeans (SB), soybean meal (SBM), soy proteins (SP), soy protein concentrate (SPC), soy protein isolate (SPI), corn gluten (CG), wheat gluten (WG), pea protein (PeaP), potato protein (PotP), fish meal (FM) and whey proteins (WP), but the results obtained are inconsistent. Differences in SID values within feed ingredients may, at least in part, be attributed to different processing conditions or inherent differences of the assay feed ingredients. Moreover, there is some evidence that the determination of SID values and $IAAL_{B}$ in piglets may be confounded by the dietary CP level of the assay diet, age and (or) body weight (BW), the level of feed intake or the methodological approach used to determine $IAAL_{B}$.

• Asian-Australasian Journal of Animal Sciences
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• 제27권7호
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• pp.990-995
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• 2014

The objective of this experiment was to determine the concentration and digestibility of crude protein (CP) and amino acid (AA) in meat meal (MM), and to compare these values with the respective values in soybean meal (SBM). Six barrows (initial body weight = $66.9{\pm}3.8kg$) surgically fitted with a T-cannula at the distal ileum were allotted to a replicated $3{\times}3$ balanced Latin square design with 3 diets and 3 periods. Two experimental diets containing test ingredients as the sole source of AA were prepared to estimate the apparent ileal digestibility (AID) for CP and AA by the direct method. An N-free diet was also prepared to estimate basal endogenous losses of CP and AA. All experimental diets contained 5% chromic oxide as an indigestible index. Each period consisted of a 5-d adaptation period and a 2-d of ileal digesta collection period. Ileal digesta samples were collected from 0900 to 1700 on d 6 and 7 of each period. The concentrations of CP, Lys, Met, and Trp in MM and SBM were analyzed to be 64.1, 3.5, 1.1 and 0.6, and 45.6, 2.8, 0.8, and 0.3%, respectively. The AID of all AA except Gly in MM was less (p<0.05) than in SBM. The AID of Lys, Met, and Trp in MM was estimated to be 56.0, 71.7, and 47.1%, respectively. The SID of all AA in MM was less (p<0.05) than in SBM. The SID of Lys, Met, and Trp was 65.1, 79.2, and 78.5%, respectively. In conclusion, the CP and AA contents in MM were greater than those in SBM whereas the ileal digestibility of all AA in MM was less than in SBM.

• Asian-Australasian Journal of Animal Sciences
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• 제25권1호
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• pp.104-113
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• 2012

Two experiments were conducted to determine the digestibility of crude protein (CP), amino acids and energy in three Chinese corn distillers dried grains with solubles (DDGS), one rice DDGS, one American corn DDGS and one American high protein distillers dried grains (HP-DDG). In Exp. 1, the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of CP and amino acids in the six samples were determined using cannulated barrows (initial BW: $43.3{\pm}1.7$ kg). In Exp. 2, the digestible energy (DE) and metabolizable energy (ME) content of these six samples were determined using crossbred barrows (initial BW: $46.0{\pm}2.5$ kg). The results of the two experiments indicated that Chinese corn DDGS is generally similar to American DDGS in chemical composition, digestibility of amino acids, DE and ME. However, Chinese DDGS had a lower Lys concentration (0.50% vs. 0.74%) and SID Lys (52.3% vs. 57.0%, p<0.01). The DE and ME values in Chinese corn DDGS were 3,427 and 3,306 kcal/kg, respectively. Rice DDGS had a similar DE and ME (3,363 and 3,228 kcal/kg) but higher Lys concentration (0.64% vs. 0.50%) to corn DDGS, while the SID of Lys was quite low (61.8%, p<0.01). HP-DDG had high value of SID of Lys, DE and ME (79.8%, 3,899 and 3,746 kcal/kg). In conclusion, except for a lower Lys concentrations and availability, the chemical composition, digestibility of amino acids, DE and ME values in Chinese corn DDGS are similar to American corn DDGS. Additionally, the rice DDGS had lower Lys content and digestible Lys values than that in corn DDGS. Thirdly, HP-DDG has higher levels of digestible amino acids and energy than DDGS.

• Journal of Animal Science and Technology
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• 제65권5호
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• pp.1014-1023
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• 2023

The aim of this study was to investigate the effect of incorporating black soldier fly (BSF) larvae and its processed form as an alternative source of protein to fish meal (FM) on the digestibility of amino acids (AA) in weaned pigs. Four cannulated pigs with an initial bodyweight of 13.25 ± 0.25 kg and aged 30 days were subjected to a 4 × 4 Latin square design with three treatments, as well as a nitrogen-free treatment. The diets used for each treatment consisted of a FM diet, a diet containing BSF larvae meal (BSFM), and a diet containing extruded BSF (BSFE). The study was conducted over four stages, with a total duration of 28 days. The apparent ileal digestibility (AID) of protein was higher in the FM treatment compared with the BSFM. Among essential AA, the AID of Arg, His, Leu, and Thr were higher in the FM compared with the BSFM and BSFE. A greater AID of Ile and Phe was observed in pigs in the FM treatment compared with the BSFM. The average AA digestibility did not show any difference between treatments. Among non-essential AA, the AID of Ala (p = 0.054) and Glu (p = 0.064) tended to be increased in the FM compared with the BSFM. Among essential AA, the standardized ileal digestibility (SID) of Arg, His, Ile, and Leu were higher in the FM compared with the BSFM. Among non-essential AA, the SID of Cys (p = 0.074) tended to be increased in the FM compared with the BSFM. In conclusion, the processing and thermal conditioning techniques utilized for BSF larvae meal showed a tendency for increased AA digestibility. Therefore, when formulating a diet, it is important to take into account the difference in AA digestibility between FM and BSFM.

• Asian-Australasian Journal of Animal Sciences
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• 제31권12호
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• pp.1946-1955
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• 2018

Objective: This study was conducted to evaluate the effect of keratinase (KE) on the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) in rice bran, cottonseed meal (CSM), rapeseed meal (RSM), corn distillers dried grains with solubles (DDGS), and peanut meal (PNM). Methods: Twelve crossbred barrows (Duroc${\times}$Landrace${\times}$Yorkshire, $50.5{\pm}1.4kg$ body weight [BW]) fitted with T-cannulas at the terminal ileum were allotted to a $12{\times}6$ Youden Square design with 12 diets and 6 periods. The treatment diets included rice bran, CSM, RSM, corn DDGS, PNM, or corn-soybean meal (cSBM) supplemented with 0.05% KE or not. Diets were given to pigs at a level of 3% BW in two equal meals. The endogenous AA losses were the mean results of three previously experiments determined by a same nitrogen-free diet fed to pigs. Pigs had free access to water during the experiment. Results: The KE supplementation improved (p<0.05) the AID and SID of Met, Thr, Val, Asp, Cys, and Tyr in rice bran. Inclusion of KE increased (p<0.05) the AID and SID of Met and Val in CSM. The KE supplementation decreased (p<0.05) the AID and SID of His in RSM and all measured AA except for Arg, Met, Trp, Val, Gly, and Pro in corn DDGS. There was an increase (p<0.05) in AID and SID of Leu, Ile, Met, Ala, Cys, Ser, and Tyr in PNM supplemented with KE compared with that without KE. Inclusion of KE increased (p<0.05) the AID and SID of crude protein, Leu, Ile, Phe, Thr, Asp, and Ser in cSBM. Conclusion: This study indicated that KE had different effects on ileal AA digestibility of feedstuffs for growing pigs, which can give some usage directions of KE in swine feed containing those detected feedstuffs.

• Animal Bioscience
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• 제34권10호
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• pp.1632-1642
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• 2021

Objective: The hypothesis was that apparent ileal digestibility (AID), basal endogenous losses, and standardized ileal digestibility (SID) of amino acids (AA) are not affected by adding acid to collection containers or bags used to collect ileal digesta from pigs. Methods: Twenty-four growing barrows (initial body weight: 77.8±4.5 kg) that were fitted with a T-cannula in the distal ileum were fed diets for three 7-d periods. An N-free diet and 3 diets containing corn, soybean meal, or wheat middlings as the sole source of AA were used. Within each period, each of the 4 diets were fed to 6 pigs. Among the 6 pigs, digesta from 3 pigs were collected in bags containing no HCl, whereas 40 mL of 3 N HCl was included in the bags used to collect digesta from the remaining 3 pigs. Every other bag collected from each pig was emptied into a container without adding HCl, whereas the remaining bags were added to a container along with 40 mL of 3 N HCl for each bag. All digesta were stored at -20℃ immediately after collection. Data were analyzed using a model that included feed ingredient, HCl in bags, HCl in containers, and all 2-way and 3-way interactions as fixed effects. No 3-way interactions were significant, and data were, therefore, reanalyzed independently for each diet as a 2×2 factorial. Results: There were no interactions between adding HCl to collection bags and to containers, and no effects of adding HCl to collection bags or containers for AID, basal endogenous losses, or SID of most AA were observed. Conclusion: It is not necessary to add acid to digesta collection bags or collection containers if ileal digesta are stored at -20℃ immediately after collection.

• Animal Bioscience
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• 제35권9호
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• pp.1400-1407
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• 2022

Objective: This research determined the chemical composition and the apparent and standardized ileal digestibility (AID and SID) of crude protein (CP) and amino acids (AA) in whole yeast and autolyzed yeast derived from sugarcane ethanol production fed to growing pigs. Methods: Six growing pigs were randomly allocated in a replicated 3×3 Latin square design with 3 diets and 3 periods of 7 days each, resulting in a total of 6 experimental replications. Three assay diets were formulated using whole yeast, autolyzed yeast, or soybean meal as the sole sources of dietary CP and AA. Pigs were allowed to adapt to the assay diets for 5 days. Thereafter, ileal digesta samples were collected continuously for 8 hours on days 6 and 7. Results: There was no difference in the chemical composition between whole yeast and autolyzed yeast, but whole yeast had low digestibility of CP and AA due to the presence of a rigid cell wall. As conducting autolysis can induce cell wall damage, the AID and SID of CP and AA were greater in autolyzed yeast than in whole yeast. Conclusion: The information obtained on the SID of CP and AA in both yeast products can be used for the accurate estimation of the bioavailability of CP and AA in feed formulations. The yeast products derived from sugarcane ethanol production are an alternative protein source in pig diets.

• Asian-Australasian Journal of Animal Sciences
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• 제21권6호
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• pp.873-882
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• 2008

Eight ileally cannulated pigs (BW $35.9{\pm}0.9kg$) were randomly allotted according to a $4{\times}3$ Latin square design to determine the effects of cellulose, pectin and starch on standardized ileal digestibility (SID) and apparent total tract digestibility (ATTD) of crude protein (CP) and amino acids (AA) as well as on the bacterial AA contribution in feces. The pigs were fed the control diet (20.2% CP, % dry matter (DM)) or one of the three experimental diets in which 25% of the control diet was substituted by cellulose, starch or pectin. Due to this substitution, dietary CP levels were lower in the cellulose (15.5% CP, % DM), pectin (15.4% CP, % DM) and starch diet (15.2% CP, % DM). Following a 15-d adaptation period, feces were collected for 5 d and ileal digesta for a total of 24 h. Starch increased SID of CP, while cellulose and pectin had no significant effect on the digestibility of CP. Overall, starch supplementation resulted in higher (p<0.05) SID values of histidine, isoleucine, threonine, alanine, aspartic acid, cysteine, glycine and serine compared with cellulose, while pectin decreased (p<0.05) SID of valine and proline compared with the starch and control diet. Both cellulose and pectin reduced (p<0.05) the ATTD of CP and AA, while starch decreased (p<0.05) ATTD of phenylalanine, alanine, proline and serine compared with the control. With regard to bacterial AA composition of the fecal mixed bacterial mass (MBM), cellulose supplementation increased (p<0.05) its content of N and almost all AA, except for valine, while pectin caused higher contents of arginine, histidine and proline compared with the control (p<0.05). The bacterial contribution of arginine in feces was higher (p<0.05) in the cellulose treatment, while pectin reduced (p<0.05) the bacterial contribution of leucine, alanine, glutamic acid and proline in feces compared with the control. In conclusion, the effects of cellulose, starch and pectin on SID were rather small. Bacterial activity in the large intestine can only explain the reduced ATTD values for arginine in the cellulose treatment, but not for the other AA in the cellulose and pectin treatments, suggesting higher endogenous losses of these AA in the large intestine.

• Asian-Australasian Journal of Animal Sciences
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• 제25권12호
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• pp.1748-1758
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• 2012

Two experiments were conducted to evaluate the effects of chemical composition of wheat shorts and red dog on energy and amino acid digestibility in growing pigs and to establish prediction models to estimate their digestible (DE) and metabolizable (ME) energy content and as well as their standardized ileal digestible (SID) amino acid content. For Exp. 1, sixteen diets were fed to thirty-two growing pigs according to a completely randomized design during three successive periods. The basal diet was based on corn and soybean meal while the other fifteen diets contained 28.8% wheat shorts (N = 7) or red dog (N = 8), added at the expense of corn and soybean meal. Over the three periods, each diet was fed to six pigs with each diet being fed to two pigs during each period. The apparent total tract digestibility (ATTD) of energy in wheat shorts and red dog averaged 75.1 and 87.9%. The DE values of wheat shorts and red dog averaged 13.8 MJ/kg (range 13.1 to 15.0 MJ/kg) and 15.1 MJ/kg (range 13.3 to 16.6 MJ/kg) of dry matter, respectively. For Exp. 2, twelve growing pigs were allotted to two $6{\times}6$ Latin Square Designs with six periods. Ten of the diets were formulated based on 60% wheat shorts or red dog and the remaining two diets were nitrogen-free diets based on cornstarch and sucrose. Chromic oxide (0.3%) was used as an indigestible marker in all diets. There were no differences (p>0.05) in SID values for the amino acids in wheat shorts and red dog except for lysine and methionine. Apparent ileal digestibility (AID) and SID values for lysine in different sources of wheat shorts or red dog, which averaged 78.1 and 87.8%, showed more variation than either methionine or tryptophan. A stepwise regression was performed to establish DE, ME and amino acid digestibility prediction models. Data indicated that fiber content and amino acid concentrations were good indicators to predict energy values and amino acid digestibility, respectively. The present study confirms the large variation in the energy content and amino acid digestibility in wheat shorts and red dog, and describes the factors that influence this variation and presents equations based on chemical composition that could probably be used to predict the DE and ME values as well as the amino acid digestibility of wheat shorts and red dog.

• 한국산학기술학회논문지
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• 제21권6호
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• pp.358-365
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• 2020

본 시험은 돼지 사료 내 단백질원으로 사용되는 원료사료의 외관상 회장 아미노산 소화율과 표준 회장 아미노산 소화율을 평가하기 위해 수행하였다. 본 시험에서 T-cannula를 회장에 설치한 자돈(Landrace×Yorkshire×Duroc, 14.4±0.35kg) 6두를 공시동물로 사용하였으며, 6×6 라틴스퀘어 설계법(Latin square design)을 이용하였다. 평가 원료사료로서 옥수수 배아, 옥수수 글루텐 밀, 옥수수 글루텐 피드, 클로렐라 및 라이신 부산물이 사용하였으며, 각 원료사료별 5일의 적응기간과 2일의 소화물 채취기간을 두었다. 라이신의 외관상 회장 소화율에서 클로렐라와 라이신 부산물이 유의적으로 높았으며(p<0.05), 각각 79.1% 및 78.7%이였다. 메치오닌의 외관상 회장 소화율은 원료사료들 간의 유의적인 차이가 없었다. 트레오닌의 외관상 회장 소화율은 클로렐라가 유의적으로 높게 나타났으며(p<0.05), 81.8%이였다. 라이신의 표준 회장 소화율에서 클로렐라, 라이신 부산물 및 옥수수 글루텐밀이 유의적으로 높게 나타났으며(p<0.05), 각각 80.4%, 79.5% 및 77.0%이였다. 메치오닌의 표준 회장 소화율은 원료사료들 간의 유의적인 차이가 없었다. 트레오닌의 표준 회장 소화율은 클로렐라가 라이신 부산물, 옥수수 글루텐 피드에 비해 유의적으로 높게 나타났으며(p<0.05), 84.2%이였다. 본 연구결과, 클로렐라와 라이신 부산물의 외관상 및 표준 회장 아미노산 소화율이 가장 우수하였으며, 이 원료들은 돼지 사료 내 단백질 대체 원료사료로서 가치가 있음을 시사한다.