• 한국미생물·생명공학회지
• /
• 제50권4호
• /
• pp.488-500
• /
• 2022

Most fungal infections by opportunistic yeast pathogens such as Candida spp. are the major causes of morbidity and mortality in patients with lowered immune. Previous studies have reported that some strains of Candida secret secondary metabolites play an important role in the decreasing of immunity in the infected patient. In this study, 110 Candida spp. were isolated from different clinical specimens from Baghdad hospitals. Candida isolates were identified by conventional methods, they were processed for Candida speciation on CHROMagar. The results of identification were confirmed by internal transcribed spacer (ITS) sequencing. Phylogenetic trees were analyzed with reference strains deposited in GenBank. Antifungal susceptibility testing was evaluated by the disc diffusion method and performed as recommended by the Clinical and Laboratory Standard Institute (CLSI) M44-A document. Candida isolates investigated produce secondary metabolites gliotoxin with HPLC technique and quantification. Out of 110 Candida isolates, C. albicans (66.36%) was the most frequent isolate, followed by the isolates of C. tropicalis (10.9%) and C. glabrata (6.36%) respectively. Concerning the antifungal susceptibility test, Candida isolates showed a high level of susceptibility to Miconazole (70.9%), Itraconazole (68.2%), and Nystatine (64.5%). The ability of obtained isolates of Candida spp. to produce gliotoxin on RPMI medium was investigated, only 28 isolates had the ability to secret this toxin in culture filtrates. The highest concentrations were detected in C. albicans (1.048 ㎍/ml). Gliotoxin productivity of other Candida species was significantly lower. The retention time for gliotoxin was approximately 5.08 min.

• 한국환경보건학회지
• /
• 제45권3호
• /
• pp.285-294
• /
• 2019

Objectives: For suitable risk management of the domestic aquatic environment, it is necessary to conduct toxicity tests using species native to Korea. In the present study, we performed toxicity ring tests using endemic freshwater arthropoda Neocaridina denticulata and evaluated its validity and reproducibility as an international standard test species. Methods: To evaluate the sensitivity levels of N. denticulata to hazardous chemicals, toxicity values for several chemicals were compared with other standard test species. Intra- and inter-laboratory acute toxicity tests were performed both within a single laboratory and among four laboratories respectively using 3,4-Dichloroaniline, which is generally used as a reference test substance in fish toxicity tests. In addition, intra- and inter-laboratory coefficient of variations (CVs) were calculated to evaluate reproducibility based on the estimated toxicity values. Results: The sensitivity of N. denticulata to several chemicals was found to be similar with D. manga, indicating that the species is valid as a test species. The CVs of the intra- and inter-laboratory tests were 22.946% with four qualified runs and 8.828% among the four laboratories, respectively. Conclusions: N. denticulata serves in an important role in the food chain of Korean aquatic ecosystems and also inhabits several other Asian countries. Since the validity and reproducibility of the species were confirmed as a toxicity test species in this study, further efforts are needed to establish N. denticulata as the international standard test species for the appropriate risk assessment of aquatic ecosystems at home and abroad.

• Nuclear Engineering and Technology
• /
• 제56권7호
• /
• pp.2875-2880
• /
• 2024

The development of nuclear power plants is in three phases. The first phase is a consideration before the decision on the NPP construction program is approved, the second phase is the preparatory work for making contracts and preparing for the construction of NPP after the NPP construction policy is approved, and the third phase is contracting, licensing and building the first NPP. As a volcanically active country, Indonesia contains over 130 active volcanoes that are part of the Pacific Ring of Fire. The volcanic aspect is one of the safety factors considered while deciding the location of an NPP. Research on the potential of natural external risks to the determination of nuclear power plants in Indonesia, including the volcanic aspect, has been conducted based on the safety reference or safety guide of the IAEA and the Nuclear Energy Regulatory Body (BAPETEN) Regulation. Due to technological advancements, safety needs have evolved so the existing Indonesia National Standard (SNI) must be updated to comply with BAPETEN regulations. The substance in SNI 18-2034-1990 relating to volcanic features seems less relevant in actual conditions, given that more complete and exact criteria for determining a site guarantee the safety and health of residents and surrounding the environment site. The study intends to conduct a gap analysis of volcanic issues in SNI and volcanic regulations. The method used is identification requirements for volcanic aspects in SNI 18-2034-1990 about Determining Site of Nuclear Reactor Guidance with BAPETEN Chairman Regulation (BCR) number 4 of 2018 about Nuclear Installation Site Evaluation Safety Provisions and BCR number 5 of 2015 about Evaluation of Nuclear Installation Sites for Volcanic Aspects, and analysis uses a qualitative method of inductive techniques. The outcome of this research applies to suggesting a revision of SNI number 18-2034-1990, especially the volcanic aspect.

• 한국산업보건학회지
• /
• 제4권2호
• /
• pp.208-223
• /
• 1994

The purpose of this study was to evaluate the efficiency of diffusive(or passive) sampler in measuring airbone organic solvents. Diffusive samplers are generally simple in construction and do not require power for operation. The efficiency of the diffusive samplers has not sufficiently been investigated in Korea. Three types of samplers were studied in this study. The sampling and analytical results by passive samplers were compared with results by charcoal tube method recommended by NIOSH(National Institute for Occupational Safty and Health). The following characteristics are identified and studied as critical to the performance passive monitors; recovery, reverse diffusion, storage stability, accuracy and precision, face velocity and humidity, n-Hexane, TCE(trichloroethylene) and toluene were used as test vapors. A dynamic vapor exposure system consisting of organic vapor generator and sampling chamber for evaluating diffusive samplers are made. The results of the study are summarized as follows. 1. NIOSH recommands that the overall accuracy of a sampling method in the range of 0.5 to 2.0 times the occupational health standard should be ${\pm}25$ percent for 95 percent confidence level. Among three types of diffusive samplers, sampler A has permeation membrane and samplers Band C have diffusive areas, samplers A and B met the criterion that overall accuracy for 95% confidence level of the samplers were within ${\pm}25$ percent of the reference value. Sampler C had overall accuracy ${\pm}9.6%$ and ${\pm}11.8%$ in hexane and TCE, respectively. The concentration of toluene was overestimated in sampler C with overall accuracy of ${\pm}43.9%$. 2. The desorption efficiencies of diffusive samplers were 96-107%. 3. There was no significant sampe loss during four weeks of storage both with and without refrigeration. 4. There was no significant reverse diffusion, when the samplers were exposure to clean air for 2 hours after sampling for 2 hours at the level of 2 TLY. 5. In case of 8 hours sampling, relative differences(RD) of concentrations between charcoal tube method and diffusive method were 15-39%, 13-46%, and 4-35% for sampler A, B and C, respectively. The performance was poor in 8 hours sampling for multiple substance monitors. 6. At high velocity(100 cm/sec), samplers B and C overestimated the concentrations of organic vapors, and sampler A with permeation membrance gave better results. 7. At 80% relative humidity, samplers showed no siginificant effect. Low humidity also did not affect the diffusive samplers.

• 한국해양학회지:바다
• /
• 제26권3호
• /
• pp.185-200
• /
• 2021

해양환경 정도관리제도는 자료의 신뢰성 확보와 측정·분석기관의 분석능력 향상을 위해 2010년부터 본격적으로 시행되었다. 시행부터 2020년까지 측정·분석기관이 정도관리제도에 참여한 누적 건수는 총 266건, 해양수산부에서 인증서를 발급한 건수는 182건, 현재까지 유효한 인증서는 42건이다. 정도관리 숙련도 평가를 위한 표준물질은 3개 분야에서 32항목이 개발되었으며, 해양환경측정망 운영, 해양오염영향조사, 해역이용영향평가, 해양심층수조사 및 어장환경조사 사업 등에서 주로 분석하는 영양염, 화학적산소요구량, 미량금속 등의 항목을 중심으로 개발되었다. 반면, 대부분의 사업에서 분석하는 해수의 수온, 염분 및 해저퇴적물의 PCBs, PAHs 등은 정도관리가 시행되고 있지 않아서 이 항목들에 대한 정도관리 확대가 필요한 시점이다. 현장 평가는 ISO/IEC 17025에 의해 평가 받은 실험실(162건)을 대상으로 품질시스템 평가 및 미흡사항을 분석하였다. 경영 및 기술요건에서는 직원들의 업무분장 미흡이 약 4.2%, 직원 교육 미흡이 약 8.7%로 취약하게 나타났다. 시험분야별로는 표준물질관리 미흡이 약 6.3%, 초자기구 세척 상태 등이 약 5.4%로 지적되었다. 미흡한 부분에 대한 문제점 및 개선점을 파악하여 제도를 지속적으로 보완해 나가야 할 것이다.

• 핵의학기술
• /
• 제19권2호
• /
• pp.112-114
• /
• 2015

Purpose Vitamin D to Anti- Rickets both steroid compounds showing activity, By acting on bone tissue secretary and the key to maintain serum Ca homeostasis. The blood level of vitamin D is the largest in D3 that the concentration of the metabolite is reflected in the holding state of vitamin D in vivo. Sunlight to change the 7-dehydrocholesterol in the skin and through the skin to D3, In the liver in combination with the D2 and D3 D4 changes. The Radioimmunoassay(RIA) method is measuring the D 3, the sensitivity can be measured also difficult trace substance to measure the normal test because it is very sensitive, but recently, a check is possible, for the Total D3 in Chemimicroparticle immunoassay(CMIA) or Chemiluminescent immunoassay(CLIA) measuring using microparticle RIA and CMIA(Architect i2000SR) / use the CLIA(DXI-800) method to compare and evaluate the correlation between the tests in the same test items. Materials and Methods Commissioned from January 2014 to March 2015 patients were enrolled in a total of 273 people. 29 out of 273 people conducted by RIA were compared with CMIA, 244 patients were compared with CLIA. Using reagents and equipment were used RIA(Diasource), CMIA(Architect i2000SR, Abbott Diagnostics) / CLIA( Unicel DXi-800, Beckman coulter). Results Correlation of the RIA and CLIA was a R2 = 0.1844 (y = 0.7303x + 3.9005), and the correlation of RIA CMIA is R2 = 0.2762 (y = 0.8862x + 4.56) respectively. (According to statistics, during the same period RIA is Deficiency 4.31%, Insufficiency 90.53%, Sufficiency 5.16%, was Excess 0%, CLIA / CMIA is Deficiency 17.02%, Insufficiency 75.91%, Sufficiency 7.03%, indicating the distribution of 0.03 % Excess) Conclusion Serum vitamin D and parathyroid hormone that show an inverse relationship, the level above which are not parathyroid hormone and vitamin D reduced the increase. The density is different for each study, at most 20 is reported to be the maximum between 30 ng / ml. In Korea it requires a proposed standard of vitamin D deficiency, reference to the WHO lack the case more than 10ng/ml, 20ng/ml and defined by the lack of, if not more than, the IOM, but looking at 12ng/ml or less to the normal to lack, at least 20ng/ml, the reference do not match the deficit under 20ng/ml, 21-29ng/ml relative lack between, was also defined as a sufficient condition for more than 30ng/ml. Although not statistically is between RIA and CLIA two ways to vitamin D levels change according to season match, when seasonally seen in summer as commonly known (April to September), winter (October to March) relative to the increase measured than it was found. Finally, the study on the correlation between the two methods have been expected to result in a consistent and apply the same view high reference value on the graph is difficult. However, there may be differences between the test equipment and methods, and could be especially the case of RIA method using an organic solvent is difficult to compare different methods and correlated view similar trend in vitamin D deficiency and quarterly aspect ratio.