• Clinical and Experimental Reproductive Medicine
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• 제45권1호
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• pp.17-24
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• 2018

Objective: To investigate sperm chromatin/DNA integrity, global DNA methylation, and DNMT mRNA transcription in men with oligoasthenoteratozoospermia (OAT) compared with normozoospermic men. Methods: Semen samples from 32 OAT patients who comprised the case group and 32 normozoospermic men who comprised the control group were isolated and purified using a standard gradient isolation procedure according to World Health Organization criteria. DNMT1, DNMT3A, and DNMT3B transcripts were then compared between groups using real-time quantitative reverse-transcription polymerase chain reaction. Global DNA methylation in sperm was determined by an enzyme-linked immunosorbent assay. Protamine deficiency and the proportion of apoptotic spermatozoa were evaluated using chromomycin A3 (CMA3), aniline blue (AB), and toluidine blue (TB) staining, as well as the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The p-values < 0.05 were considered to indicate statistical significance. Results: Significantly higher proportions of AB+, TB+, CMA3+, and TUNEL+ spermatozoa, as well as DNMT3A and DNMT3B transcription, were found in the OAT group. Positive correlations were detected between sperm parameters, DNA/chromatin damage, and DNMT3A and DNMT3B transcripts. Global DNA methylation was significantly higher in the OAT patients and had a significant correlation with abnormal results of all sperm chromatin integrity tests, but was not associated with DNMT1, DNMT3A, or DNMT3B expression. Conclusion: Oligoasthenoteratozoospermic men showed abnormal sperm parameters, abnormal chromatin/DNA integrity, and a higher global DNA methylation rate, as well as overexpression of DNMT mRNA.

• Journal of Dental Anesthesia and Pain Medicine
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• 제16권2호
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• pp.111-116
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• 2016

Background: Inferior alveolar nerve block (IANB) of the mandible is commonly used in the oral cavity as an anesthetic technique for dental procedures. This study evaluated the success rate of the first IANB administered by dental practitioners. Methods: Volunteer dental practitioners at Mahidol University who had never performed an INAB carried out 106 INAB procedures. The practitioners were divided into 12 groups with their advisors by randomized control trials. We recorded the success rate via pain visual analog scale (VAS) scores. Results: A large percentage of the dental practitioners (85.26%) used the standard method to locate the anatomical landmarks, injecting the local anesthetic at the correct position, with the barrel of the syringe parallel to the occlusal plane of the mandibular teeth. Further, 68.42% of the dental practitioners injected the local anesthetic on the right side by using the left index finger for retraction. The onset time was approximately 0-5 mins for nearly half of the dental practitioners (47.37% for subjective onset and 43.16% for objective onset), while the duration of the IANB was approximately 240-300 minutes (36.84%) after the initiation of numbness. Moreover, the VAS pain scores were $2.5{\pm}1.85$ and $2.1{\pm}1.8$ while injecting and delivering local anesthesia, respectively. Conclusions: The only recorded factor that affected the success of the local anesthetic was the administering practitioner. This reinforces the notion that local anesthesia administration is a technique-sensitive procedure.

• 대한간호학회지
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• 제7권1호
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• pp.1-9
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• 1977

Primary function of health record is that as tool of communication between the health processionals with the mutual goal, the promotion of health care standard. Studies have been carried out world over oil tile subject, among those, Weed's Problem-Oriented Health Record is considered a paramount achievement. This study was designed to assess tile possibility of implementing tile problem-oriented health record system through ail experiment in order to provide data for nurse administrators infiltrating reformation of recording system and format. Record of 29 patients admitted at Korea University Hospital, Seoul, from March through June, 1976 for 4 to 14 days were sampled. Nursing notes were recorded by research assistants; senior nursing student trailed extensively by the researcher oil Problem-Oriented Records, oil Problem Oriented Nursing Record format (experimental group) and analysis were carried out comparative, with that of traditional nursing records noted by other nursing personnel (control group) on the same patient. Attitude towards Problem Oriented Nursing Record system and format were attained through questionaries responded by the 51 research assistants. Results are as fellows: Comparative analysis revealed that: 1. Assessment of patients' health problems recorded significantly more in traditional records. 2. Focus of health Problem differed; traditional records slowed significantly higher frequency in medical and procedure as focus while problem oriented records on nursing focus problems. 3. Problem- Oriented records were better organized, Mean value scores of attitude towards Problem- Oriented Records revealed that: Positive value scores on all 4 categories: 1) Assessment of nursing needs, 2) Nursing care planning 3) Patient progress assessment and 4) Tool of teaching and learning revealed that the Problem-Oriented Nursing Record is positively accepted by tile respondents. Recommendation Further experiments on implementation of Problem- Oriented Health Record are recommended: experiment involving all health professionals, in larger scope and longitudinal.

• 한방재활의학과학회지
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• 제27권2호
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• pp.39-54
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• 2017

Objectives This study aimed to search the Chinese literature on acupotomy for lumbar disc herniation and to analyze their methodologies. Methods Using 4 Chinese databases (CBM [www.sinomed.ac.cn], CNKI [www.cnki.net], WANFANG [www.wanfangdata.com], and WEIPU [www.cqvip.com]), we searched for clinical trials conducted in China over the past 10 years on acupotomy for lumbar disc herniation. The search terms used were "(腰椎椎間板脫出症 or 椎間板脫出症 or 椎間板脫出) and (针刀or 针刀松解术)" and we selected only meta-analyses that were published before December 2016. From among these meta-analyses, we excluded duplicates and selected the remaining 36 randomized controlled trials (RCTs) for our final analysis. Results The largest numbers of acupotomy papers were published in 2008 and 2013 (8 papers each). The average number of subjects was 120; the most common treatment method used for the control group was standard acupuncture (in 11 papers), and the most common concurrent treatment in the treatment group was massage (in 10 papers). The most common site of needle insertion was between the spinous processes, or at less than 0.5 cm on either side of the spinous processes (in 24 papers). The most common site of adhesion lysis was at the transverse process (in 24 papers). Two studies were blinded, and the mean Jadad score was 1.17. Conclusions A large number of RCTs on the use of acupotomy for lumbar disc herniation are conducted every year in China, and the procedure has been shown to be very effective, with few adverse effects. However, the average quality of the studies was not very high. Based on our study, we expect several high-quality clinical trials on acupotomy to be conducted in Korea in the future.

• 한국군사과학기술학회지
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• 제14권3호
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• pp.423-431
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• 2011

Warfighting experimentation is an important process for identifying requirements against changing military environment and for verifying proposed measures for reforming military service. The wargame simulation experiment is regarded as one of the most effective means to warfighting experimentation, and its importance is increasing than ever. On the other hand, the results of wargame experiments could be unreliable due to the uncertainty involved in the experimental procedure. To improve the reliability of the experimental results, systematic experimental procedures and analysis methods must be employed, and the design and analysis of experiments technique can be used effectively for this purpose. In this paper, AWAM, a wargame simulator, is used to optimize the organization of operational troops. The simulation model describes a warfighting situation in which the 'survival rate of our force' and the 'survival rate of the enemy force' are considered as responses, 'the numbers of weapons in the squad' as control factors, and 'the uncontrollable variables of the battlefield' as noise factors. In addition, for the purpose of effective experimentation, the product array approach in which the inner and outer orthogonal arrays are crossed is adopted. Then, the signal-to-noise-ratio for each response and the desirabilities for the means and standard deviations of responses are calculated and used to determine a compromise optimal solution. The experimental procedures and analysis methods developed in this paper can provide guidelines for designing and analyzing wargame simulation experiments for similar warfighting situations.

• 융합보안논문지
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• 제13권6호
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• pp.35-41
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• 2013

의료정보 보안에 대한 대안은 무엇보다 의료정보의 안전한 보존과 각종의 보안 위협으로부터 안전성을 강구하는 소프트웨어 설계로부터 시작되어야 한다. 의료정보시스템은 산재된 의료정보를 실시간으로 통합할 수 있어야 하고 의료정보의 교환은 신뢰할 수 있는 데이터 통신이어야 한다. 의료정보시스템의 소프트웨어 아키텍처 설계는 의료정보 공유상 보안 문제를 파악하고 의사소통을 통해 사용자 요구사항을 파악하여 소프트웨어 설계에 반영한다. 소프트웨어 프레임워크 설계, 메시지 표준 설계, 웹기반 프로세스간 통신절차 설계, 접근제어 알고리즘 설계, 아키텍처 기술서 작성, 아키텍처 평가의 제반 절차를 정립한다. 소프트웨어 아키텍처 초기 결정은 설계, 개발, 테스트, 유지보수에 지속적인 영향을 미친다. 또한 프로젝트상의 세부적 의사결정 근거가 된다. 의료정보보안 기반 소프트웨어 아키텍처 설계방법은 오늘날 중요한 과제가 되고 있는 의료정보 보안의 기본틀을 제공할 수 있을 것이다.

• 대한본초학회지
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• 제35권4호
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• pp.45-50
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• 2020

Objective : This study was performed to evaluate the toxicity after a single oral administration of black raspberry extract to male and female Sprague-Dawley (SD) rats and to determine the approximate lethal dose (ALD). Methods : We previously showed that the black raspberry extract repressed the simvastatin-mediated expression of Proprotein convertase subtilisin/kexin type 9 (PCSK9) and improved Low-Density Lipoprotein cholesterol (LDL-C) uptake by hepatocytes through the induction of the Low-Density Lipoprotein Receptor expression in hepatocytes. The groups consisted of black raspberry extract groups, as an oral dose of 2,000 mg/kg and a control group. 5 weeks SD rats were randomly assigned to 4 groups of 5 rats. Each male and female SD rats were administered orally once. For 14 days after the administration, mortality, clinical signs, changes in body weight, and necropsy findings were observed according to the "Standard for Toxicity Study of Pharmaceuticals" of Korea Food and Drug Administration (KFDA) guideline and "Acute Oral Toxicity- Fixed Dose Procedure" of OECD Test Guideline. Results : There were no cases of mortality in the group administered with 2,000 mg/kg of male and female, and no abnormalities in body weight change and clinical signs. Also, no gross abnormalities were observed at the autopsy. Conclusions : As a result of a single oral administration of the black raspberry extract to SD rats, the ALD was determined to exceed 2,000 mg/kg for both male and female SD rats.

• 분석과학
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• 제18권6호
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• pp.475-482
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• 2005

화학분석 분야에는 ISO 17025에 따른 체계적 품질 관리/보증의 요구가 커지면서, 측정 불확도의 표기가 크게 요구되고 있다. 측정품질의 정량화를 위하여, 화학분석의 QA/QC 문헌들로부터 화학분석 품질에 영향을 미치는 중요한 불확도 요소를 선정하였고, 이것을 측정결과 평가 요소의 기본인 7개의 품질요소로서 반복성, 직선성, 소급성, 변동성, 매질효과, 균질성, 안정성으로 구분하였다. 화학분석의 QA/QC를 위한 이 7대 품질 요소의 측정 모델화, 표준불확도의 계산 및 합성방법을 각각 예로서 제시하였다.

• 한국재난정보학회 논문집
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• 제15권1호
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• pp.98-108
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• 2019

연구목적:본 연구는 화재 시 샌드위치패널 구조 창고의 벽 천장접합부 방호를 위한 스프링클러 성능확인 실험을 목적으로 한다. 연구방법: 현장조사결과를 기반으로 창고의 공간규격과 가연물을 선정하고 벽 코너 및 헤드 직하부에서의 방호실험을 수행하고 결과를 분석하였다. 연구결과:벽 코너 화재 시나리오에 대해서 K-80 폐쇄형 스프링클러헤드의 작동으로 샌드위치 패널의 착화 및 접합부 손상이 방지됨을 확인하였다. 결론:샌드위치패널 심재로의 착화 및 연소방지를 통한 화세제어로 이를 가정한 소방대의 소화 및 구조활동을 위한 표준진압대책이 마련되어야 한다.

• 한국컴퓨터정보학회논문지
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• 제26권1호
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• pp.209-216
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• 2021

본 연구는 간호학생 실습교육을 위해 시나리오 기반 입원관리 가상현실(Scenario-based Admission Management Virtual Reality, 이하 SAM VR) 콘텐츠를 개발한 후 효과성을 검증하기 위해 수행되었다. 연구에 사용된 SAM VR 콘텐츠는 한국간호교육평가원에서 제시한 표준실무 절차에 따라 기어 VR과 스마트폰을 활용하여 10분 분량으로 개발되었다. SAM VR 콘텐츠 활용한 실습교육을 받은 실험군 30명은 실습교육 후에 학습몰입감, 학습자신감 및 학습만족도가 통계적으로 유의하게 상승했다(p<.001). 전통적인 방식으로 실습교육을 받은 대조군은 실습교육 후에 학습자신감은 상승하였으나(p<.005) 학습몰입감과 학습만족도에는 변화가 나타나지 않았다(p>.005). SAM VR 콘텐츠는 간호학생의 학습몰임감, 학습자신감과 학습만족도에 효과적인 실습교육 콘텐츠임이 검증되었다.