• Journal of the Korean Institute of Telematics and Electronics
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• v.24 no.6
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• pp.1080-1086
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• 1987

SLAGEN, a system for symbolic cell layout based on artificial intelligence approach, takes as input a transistor connection description of CMOS standard cells and environment information, and outputs a symbolic layout description. SLAGEN performas transistor grouping by a heuristic search method, in order to minimize the number of separations, and then performs group reordering and transistor reordering with an eye toward minimizing routing. Next, SLAGEN creates a rough initial routing in order to guarantee functionality and correctness, and then improve the initial routing by a rule-based approach.

• Korean Journal of Microbiology
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• v.2 no.1
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• pp.1-11
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• 1964

Yung Nok Lee (Dept. of Biology, Korea University) : Studies on the phosphate metabolism in Chlorella, with special reference to polyphosphate. Kor. J. Microbiol., Vol.2, No.1, p1-11 (1964). 1. Uniformly $^{32}P$-labeled Chlorella cells which were irradiated with Cobalt-60 gamma-rays of about 70, 000 $\gamma$ dose, were further grown in a standard "cold" medium ("hot".rarw."cold"), and some portions of the algae were taken out at the begining of, and at intervals during the culture, and subjected to analyze the contents of $^{32}P$- and total P in various fractions of the cell materials. Results obtained were compared with those of nonirradiated normal cells. 2. Amounts of phosphate in various fractions of the nonirradiated normal Chlorella cells were measured using uniformly $^{32}P$--labeled cells. Analysis of the $^{32}P$--labeled algal cells showed that the highest value in P-content was the fraction of RNA followed by those of lipid, polyphosphate "C" polyphosphate "B", DNA, nucleotidic labile phosphate compounds, polyphosphate "A" and protein. It was observed that content of total polyphosphates in a single Chlorella cell was almost equal to RNA-P content in the cell, and the amount of RNA-P was almost equal to ten times of DNA-P content. 3. When the $^{32}P$--labeled algae which were irradiated with gamma-rays were grown in a normal "cold" medium, phosphate contents in the fraction of DNA, nucleotidic labile phosphate compounds and protein decreased markedly, while the contents of phosphate in the fractions of polyphosphate "C" and potyphosphate "B" increased in comparison with those of unirradiated normal cells. So, it was considered that the pretreatment of above mentioned dose of gamma-ray inhibited DNA and protein synthesis from polyphosphate in Chlorella cells. 4. Proceeding the culture of $^{32}P$--labeled Chlorella in a "cold" standard medium, whose synthetic activity of DNA and protein from polyphosphate was disturded by gamma-ray irradiation, the amounts of $^{32}P$-in the fraction of polyphosphate "C" increased, in contrast with those of polyphosphate "B" fraction. According to these experimental results, it was inferred that polyphosphate "B" could transform into polyphosphate "C" in normal growing Chlorella cells.sults, it was inferred that polyphosphate "B" could transform into polyphosphate "C" in normal growing Chlorella cells.ing Chlorella cells.

• Journal of the Korean Institute of Telematics and Electronics
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• v.23 no.4
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• pp.557-564
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• 1986

This paper describes a standard cell placement strategy which consists of three kconsecutive steps` initial placement, iterative placement improvement, and string placement. In the initial placement step, cell placement was done by solving the linear ordering problem for a one-dimensional layout of standard cells and then zigzaging the resultant linear order width in the chip plane. The iterative placement improvement step is based on the iterative pairwise interchange using the estimated total routing length as a figure-or -merit. The string placement is used to reorder cells and terminals in each etandard cell row such that channel routing in the adjacent channels is not blocked by cyclic constraints and needs fewer routing tracks. The placement program is coded in PASCAL and kimplemented on a VAX-11/750 computer. Experimental results for several examples are included.

• Korean Journal of Microbiology
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• v.4 no.1
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• pp.14-20
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• 1966

1. Uniformly $^{32}P$-labeled Chlorella cells were further grown in a standard "cold" medium and aliquots of the algal cells were taken out at the beginning of, and at intervals during the culture, and subjected to analyze the contents of $^{32}$ P and total P in various fractions of the cell constituents. 2. When the $^{32}P$--labeled algae were grown in a normal "cold" medium, the P-contents in the fractions of DNA and protein increased. In the meantime the $^{32}P$- in acid-insoluble polyphosphate fraction decreased considerably, while that in RNA-polyphosphate complex significantly increased. 3. It was inferred that, under the experimental conditions of the present study, the phosphorus in polyphosphate seems to be transferred to RNA polyposphate complex and the phosphorus used in the synthesis of DNA and protein was, directly or indirectly, taken from those fractions above.ose fractions above.

• Journal of the Korean Institute of Telematics and Electronics
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• v.24 no.6
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• pp.1074-1079
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• 1987

We present a fast, constructive algorithm for the automatic placement of standard cells, which consists of two steps. The first step is responsible for cell-row assignment of each cell, and converts the circuit connectivity into a multi-stage graph under to constraint that sum of the cell-widths in each stage of the multi-state graph does not exceed maximum cell-row width. Generatin of feed-through cells in the final layout was shown to be drastically reduced by this step. In the second step, the position of each cell within the row is determined one by one from left to right so that the cost function such as the local channel density is minimized. Our experimental result shows that this algorithm yields near optimal results in terms of the number of feed-through cells and the horizontal tracks, while running about 100 times faster than other iterative procedures such as pairwise interchange and generalized force directed relaxation method.

• 한국정보디스플레이학회:학술대회논문집
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• 2002.08a
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• pp.567-570
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• 2002

The LVDS (Low Voltage Differential Signaling) I/O cells, fully compatible with ANSI TIA/ EIA-644 LVDS standard, are designed using a 0.35${\mu}m$ standard CMOS technology. With a single 3V supply, the core cells operate at 1.34Gbps and power consumption of the output driver and the input receiver is 10. 5mW and 4.2mW, respectively. In the output driver, we employ the DCMFB (Dynamic Common-Mode FeedBack) circuit which can control the DC offset voltage of differential output signals. The SPICE simulation result of the proposed output driver shows that the variation of the DC offset voltage is 15.6% within a permissible range. In the input receiver, the proposed dual input stage with a positive feedback latch covers rail-to-rail input common-mode range and enables a high-speed, low-power operation. 5-channels of the proposed LVDS I/O pair can handle display data up to 8-bit gray scale and UXGA resolution.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39A no.7
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• pp.389-397
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• 2014

This paper focuses on advanced receiver for interference suppression or cancellation from neighbor cells in the 3GPP Rel-12 standard. From UE (User Equipment) perspective, the advanced receiver which can manage inter-cell interference of adjacent cell is required to support and manage fast-growing wireless data traffic. In 3GPP standard, NAICS which is one of approaches to improve SINR and receiver performance by directly suppression or cancellation of data (PDSCH) and control (PDCCH) signal from interference cells is discussed. In this paper, we briefly introduce the concept and candidate receivers for NAICS (Network Assisted Interference Cancellation and Suppression) based on 3GPP Rel-12 standard, and simulation results for basic NAICS receiver performance are provided.

• Biomolecules & Therapeutics
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• v.24 no.1
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• pp.49-56
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• 2016

To determine the protective effect of aloe-emodin (AE) from high glucose induced toxicity in RIN-5F (pancreatic ${\beta}$-cell) cell and restoration of its function was analyzed. RIN-5F cells have been cultured in high glucose (25 mM glucose) condition, with and without AE treatment. RIN-5F cells cultured in high glucose decreased cell viability and increased ROS levels after 48 hr compared with standard medium (5.5 mM glucose). Glucotoxicity was confirmed by significantly increased ROS production, increased pro-inflammatory (IFN-${\gamma}$, IL-$1{\beta}$,) & decreased anti-inflammatory (IL-6&IL-10) cytokine levels, increased DNA fragmentation. In addition, we found increased Bax, caspase 3, Fadd, and Fas and significantly reduced Bcl-2 expression after 48 hr. RIN-5F treated with both high glucose and AE ($20{\mu}M$) decreased ROS generation and prevent RIN-5F cell from glucotoxicity. In addition, AE treated cells cultured in high glucose were transferred to standard medium, normal responsiveness to glucose was restored within 8hr and normal basal insulin release within 24 hr was achieved when compared to high glucose.

• Proceedings of the Optical Society of Korea Conference
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• 2009.02a
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• pp.293-294
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• 2009

The spectral responsivity of solar cells has been measured under high background irradiance using an LED-based differential spectral responsivity Comparator (DSR-C). The comparator developed is fully automated and has some advantages: It does not need a chopper to modulate the light. Unlike the conventional method, it does not require a monochromator to select wavelength. It covers a wavelength range up to 1200 nm. The wavelength range of the comparator is limited by the spectral power distribution of the LEDs and the spectral responsivity of the standard detector. An active temperature control was utilized to meet the specified standard conditions of solar cell test. This work shows the effect of different levels of background irradiance on the spectral responsivity and the importance of same background irradiance for solar cell test as specified by the corresponding standard.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.387-393
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• 2011

The objective of this study was to develop a real-time PCR method for the rapid detection and quantification of the protozoan pathogen Perkinsus olseni using a TaqMan probe. For the standard, genomic DNA was extracted from $10^5$ in vitro-cultured P. olseni trophozoites, and then 10-fold serial dilutions to the level of a single cell were prepared. To test the reliability of the technique, triplicates of genomic DNA were extracted from $5{\times}10^4$ cells and 10-fold serial dilutions to the level of 5 cells were prepared. The standards and samples were analyzed in duplicate using an $Exicycler^{TM}$ 96 real-time quantitative thermal block. For quantification, the threshold cycle ($C_T$) values of samples were compared with those obtained from standard dilutions. There was a strong linear relationship between the $C_T$ value and the log concentration of cells in the standard ($r^2$ = 0.996). Detection of DNA at a concentration as low as the equivalent of a single cell showed that the assay was sensitive enough to detect a single cell of P. olseni. The estimated number of P. olseni cells was similar to the original cell concentrations, indicating the reliability of P. olseni quantification by real-time PCR. Accordingly, the designed primers and probe may be used for the rapid detection and quantification of P. olseni from clam tissue, environmental water, and sediment samples.