• 제목/요약/키워드: Stain Analysis

검색결과 292건 처리시간 0.038초

주조/단조 기술을 이용한 대형 알루미늄 플랜지 개발에 관한 연구 (A Study on the Development of Large Aluminum Flange using Casting/Forging Process)

  • 배원병;왕신일;서명규;조종래
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 2001년도 춘계학술대회 논문집
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    • pp.905-909
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    • 2001
  • The significance of casting/forging process for reducing the production cost of large components is being noted in these days. This casting/forging process is a method of forging a workpiece preformed by casting into the final shape. In this study, the casting/forging process has been applied in manufacturing a large aluminum flange in order to determine the optimum forging condition of the aluminum flange. The optimum range of forging temperature of Al 5083 was from $420^{\circ}C$ to $450^{\circ}C$. The suitable strain rate was 1.5 $sec^{-1}$. The deformation amount of a preform in a forging process is key role in the mechanical properties of casting/forging products. In order to find the change of mechanical properties according to effective stain of cast aluminum billets, a hot upsetting test were performed with rectangular blocks and then a uniaxial tensile test was performed with specimens cut from the upsetted billets. The tensile strength and the elongation of cast/upsetted aluminum billets were increased largely until the effective strain was 0.7. FE analysis was performed to determine the configurations of cast preform and die for an aluminum flange. In the FE analysis, the forging load-limit was fixed 1500ton for the low equipment cost. The cast preform was designed so that the effective stain around the neck of a flange exceeds 0.7. In the forging experiment for an aluminum flange, it was confirmed that the optimal configuration of the cast preform predicted by FE analysis was very useful. The cast/forged products using designed preform were made perfectly without any defects.

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랫드의 간발암화과정에서 분리한 간세포의 ploidy 분포변화에 관한 연구 (Studies on the ploidy distributions of the hepatocytes isolated in hepatocarcinogensis of rats)

  • 최경철;이영순
    • 대한수의학회지
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    • 제32권4호
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    • pp.649-661
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    • 1992
  • This study was performed to compare DNA content by flow cytometer (FCM) and glutathione S-transferase placental form (GST-P) positive foci for searching objective and accurated properties of tumor. Sprague-Dawley rats aged six weeks were divided into three groups and group 1 and 2 of rats were given an intraperitoneal injection of diethylnitrosamine at 200mg/kg body weight and group 3 of rats were given saline. Three weeks after beginning of the experiment, all groups were performed partial hepatectomy. Group 1 of rats were begun to feed on diets containing 0.02% 2-acetylaminofluorene as a promoter for six weeks, group 2 and 3 of rats were begun to feed on basal diets. At 4, 6, and 8 weeks after initiation, all groups of rats were killed, livers were extracted for H & E stain, immunohistochemical stain, and DNA ploidy analysis. In quantitative analysis for GST-P positive lesion number and area by using Image Analyzer, group 1 and 2 represented significant difference in comparison with group 3. In ploidy distribution, diploid cells of group 1 and 2 were increased significantly in comparison with those of group 3 at 4, 6, and 8 weeks after initiation, respectively tetraploid cells were reduced. But S-phase cells were not changed significantly. It is concluded that ploidy change by FCM is useful as objective data for early detection in hepatocarcinogenesis. Therefore, methodology and study of DNA content are carried out for more objective and accurate ploidy analysis in liver tumor.

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치위생과정에 근거한 구강건강관리프로그램 대상자의 치위생계획의 영향요인 분석 (Analysis of the factors of dental hygiene plans influencing patients of the dental hygiene program based on dental hygiene process)

  • 김유린
    • 한국치위생학회지
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    • 제18권2호
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    • pp.227-237
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    • 2018
  • Objectives: This study aims to recognize the importance of dental hygiene process diagnosis of dental hygiene process which can comprehensively grasp the patient's problem and to use it as a basis for establishing the patient's preventive treatment plan. Methods: This study did survey to 443 patients who received treatment based on the oral health care program from a dental clinic in Busan from January 2015 to January 2017. Data analysis was performed using IBM SPSS Statistics (Version 21.0), and statistical significance level was set at ${\alpha}=0.05$. Binary logistic regression analysis was performed to the dental hygiene problems affecting the dental hygiene plan. Results: There were significant differences in dental hygiene problems between male and female respondents on various dental problems such as dental plaque deposition, attrition, stain, dental fear, possibility of jaw joint disorder, food pressing, possibility of malocclusion. There were also significant differences in dental hygiene plans between male and female respondents in air-Jet, non-smoking education, and sealant. The most common dental hygiene plan was scaling, The problem of stain showed that the scaling plan was 0.20 times less. The explanatory power of the model was 43.5%, and the Hosmer and Lemeshow tests were 0.345. Conclusions: Therefore, if we continue to study the factors affecting the dental hygiene problems and the plan, we can reduce the burden of the dental hygienists applying the dental hygiene process in the dental clinic. And, it is expected that the oral health care program using the dental hygiene process will spread to the dental clinic as an excellent oral preventive program.

Comparison of fracture strength, surface hardness, and color stain of conventionally fabricated, 3D printed, and CAD-CAM milled interim prosthodontic materials after thermocycling

  • Mesut Yildirim;Filiz Aykent;Mahmut Sertac Ozdogan
    • The Journal of Advanced Prosthodontics
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    • 제16권2호
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    • pp.115-125
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    • 2024
  • PURPOSE. The purpose of this in vitro study was to investigate the fracture resistance, surface hardness, and color stain of 3D printed, CAD-CAM milled, and conventional interim materials. MATERIALS AND METHODS. A total of 80 specimens were fabricated from auto polymerizing polymethyl methacrylate (PMMA), bis-acryl composite resin, CAD-CAM polymethyl methacrylate resin (milled), and 3D printed composite resin (printed) (n = 20). Forty of them were crown-shaped, on which fracture strength test was performed (n = 10). The others were disc-shaped specimens (10 mm × 2 mm) and divided into two groups for surface hardness and color stainability tests before and after thermal cycling in coffee solution (n = 10). Color parameters were measured with a spectrophotometer before and after each storage period, and color differences (CIEDE2000 [DE00]) were calculated. The distribution of variables was measured with the Kolmogorov Smirnov test, and one-way analysis of variance (ANOVA), Tukey HSD, Kruskal-Wallis, Mann-Whitney U tests were used in the analysis of quantitative independent data. Paired sample t-test was used in the analysis of dependent quantitative data (P < .05). RESULTS. The highest crown fracture resistance values were determined for the 3D printed composite resin (P < .05), and the lowest were observed in the bis-acryl composite resin (P < .05). Before and after thermal cycling, increase in mean hardness values were observed only in 3D printed composite resin (P < .05) and the highest ΔE00 value were observed in PMMA resin for all materials (P < .05). CONCLUSION. 3D printing and CAD-CAM milled interim materials showed better fracture strength. After the coffee thermal cycle, the highest surface hardness value was again found in 3D printing and CAD-CAM milled interim samples and the color change of the bis-acryl resin-based samples and the additive production technique was higher than the PMMA resin and CAD-CAM milled resin samples.

초음파 스캔에서 알코올 겔 적용 시 화질과 소독효과 분석 (Analyzing Image Quality and Disinfection Effectiveness of Alcohol Gel Application in Ultrasound Scans)

  • 김현주
    • 한국방사선학회논문지
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    • 제18권4호
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    • pp.365-372
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    • 2024
  • 초음파 스캔에서 알코올 겔 적용 시 화질과 소독 효과 분석을 위해 초음파 겔과 알코올 함유량이 다른 소독용 알코올 겔을 변경 적용하여 실험하였다. 화질 분석은 ATS-539 다목적 팬텀을 이용하여 사각 구역, 축 및 측 방향 분해능, 민감도, 회색 조의 변화 항목을 비교해 보았고, 소독 효과 분석은 임상에서 사용 중인 볼록형 탐촉자 3개의 덮개 면을 swab을 통해 검체 수집과 24시간 증균배양한 후 gram stain과 identification을 하였다. 화질 분석 결과 알코올 함유량과 상관없이 모든 항목에서 초음파 겔 적용 영상과 차이가 없는 영상으로 구현되었으며, 모든 탐촉자에서 staphylococcus aureus, streptococcus, enterococcus 등의 병원균이 검출되었고 소독용 알코올 겔 적용으로 모두 사멸하였다. 초음파 검사 시 알코올 겔 적용은 화질 유지와 소독 효과 측면에서 의미가 있었으며 본 연구결과를 바탕으로 지속적인 후속 연구를 진행하고 보완하여시 제품화한다면 초음파 겔 대체재로 알코올 겔 적용은 화질뿐만 아니라 감염 예방 측면까지 고려할 수 있어 임상 검사 시 매우 유용할 것으로 판단된다.

Applications of Geostatistics to the Quantitative Analysis of Genetic Instability in Carcinogenesis

  • Kim Hyoung-Moon
    • Communications for Statistical Applications and Methods
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    • 제13권1호
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    • pp.167-175
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    • 2006
  • It has long been recognized that cancer is a genetic disease. To find this measures of genetic instability, stain cells with chromosome specific probes using chromosome in-situ hybridization technique is adopted. Even though in-situ hybridization technique is powerful, truncation of nuclei often results in under-representation of chromosome copies in slides due to the sectioning of tissue blocks. Because of this problem we suggest three different methods to analyze the cervical cancer data set. We observe that genetic instability is an increasing function of histology and our suggested model is the best in detecting genetic instability of tumorigenesis processes.

현장계측 및 수치해석에 의한 장대버팀보의 온도영향 분석 (The Analysis of the Temperature Effects on Long-span Strut by Field Monitoring and Numerical Analysis)

  • 이문주;최성근;신세영;이우진
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2005년도 춘계 학술발표회 논문집
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    • pp.1468-1475
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    • 2005
  • In this study, the effects of temperature on loading of long-span strut were studied by using field monitoring and numerical analysis. For the field monitoring, several sensors, such as stain gages, temperature gages and load cells, were installed on the struts. From the monitoring results, the relation between temperature and axial force of the struts was analysed. By numerical analysis, the changes of axial force of strut and lateral displacement of wall due to temperature change were described with the strut length and ground conditions.

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Electrophoretical Analysis of 36-Kilodalton Outer Membrane Protein of Vibrio vulnificus ATCC 27562

  • Moon-Soo Heo;Cho-Rok Jung
    • Journal of Life Science
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    • 제9권1호
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    • pp.35-39
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    • 1999
  • Elecrophoreticl analysis of a 36 kDa protein was runned by SDS-PAGE, isoelectric focusing (IEF) and two dimensional electrophoresis pattern. Major 36 kDa and 25, 46, 48, 66 kDa protein were detected by Coomassie blue stain on SDS-PAGE. Major 36kDa protein was eluted for production of antiserum for serological analysis, IEF and two dimensional electrophoresis. Isoelectric point of 36kDa was aout pH 8.5. Two dimensional electrophoresis of eluted 36kDa showed one point on the gel. Anti-36 kDa serum made by newzilland rabbit for serological test. In ELISA, final titer of antibody was 100×{TEX}$2^5}${/TEX} : 1. Neutralize ability of serum was examined by slide agglutination test and colonization test in rat. Anti-36 kDa serum agglutinated whole cell of V. vulnificus were inhibited colonization on intestine in rat. Accordingly In this paper contain some electrophoretical analysis and serological test of a 36 kDa OMP of V. vulnificus.

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한국산 다람쥐 핵형의 비교연구 I.일반염색과 C-Banding방법에 의한 한국산 청서(Sciurus vulgaris corea) 와 다람쥐(Tamias sibiricus asiaticus)의 핵형 분석 (A Comparative Karyotype Study in Korean Squirrels. I Karyotype Analysis of Sciunis vulgaris coreae and Tamlas sibiricus asiaticus by Conventional Giemsa Staining and C-Banding Method)

  • 김종봉;이희영
    • 한국동물학회지
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    • 제33권2호
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    • pp.222-230
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    • 1990
  • 한국산 청서(Sciunis vulgaris corea)와 다람쥐(Tamias sibiricus asiaticu)의 핵형을 일반 Giemsa- stain과 C-banding stain방법으로 분석하였다. 청서의 염색체 수는 2n=40이였으며 이 중 6쌍은 중부, 8쌍은 차중부, 3쌍은 차단부 그리고 2쌍은 단부 염색체이었고 X 염색체는 차중부,Y염색체는 acro또는 차단부 염색체로서 NF(arm number)=72(성염색체 제외)를 나타내었다. 다람쥐의 염색체 수는 2n=38이었으며 이 중 3쌍은 중부, 4쌍은 차중부, 5쌍은 차단부, 그리고 6쌍은 단부 염색체이었고 X염색체는 차중부, Y염색체는 중부염색체로서 NF=60을 나타내었다. C-banding분석결과, 청서의 염색체에서는 각 경우 대부분 동원체 (centromere)부위와 말단부(telomere)에 주로 구조적이질염색질이 분포하였고 다람쥐에서는 몇몇 염색체상(제2,3,9번)을 제외하고는 주로 동원체 부위에 구조적이직염색질이 분포하였다. 이러한 결과들로 보아 핵형상의 분화에 non-Robensonian 재배열과 구조적 이질염색질의 분포가 중요한 작용을 한 것으로 생각된다.

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