• Journal of Plant Biotechnology
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• 제45권3호
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• pp.257-265
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• 2018

감미료(sweetener)는 단맛을 느끼게 하는 첨가물 중 하나로 인공감미료와 설탕이 대표적이며, 단맛의 특성을 지닌 감미 단백질도 잘 알려져 있다. 본 연구는 천연 감미 단백질, brazzein, thaumatin 및 miraculin의 안정적인 생산을 위해 Agrobacterium방법으로 상추 세포를 형질 전환시켰다. 감미 단백질을 코딩하는 합성 유전자들은 상시적으로 발현하는 프로모터의 제어 하에 상추에 형질전환 하였다. 형질전환한 잎을 이용하여 RT-PCR 및 Western blot 분석을 실시한 결과 감미 단백질이 안정적으로 발현하는 것을 확인하였다. 형질전환 상추에서 발현한 단백질은 단맛을 유발하는 단백질 활성을 가지고 있었다. 이러한 결과는 유전자 재조합 감미 단백질들은 형질전환한 상추에 잘 발현된 것을 보여 주며, 이들 생산 시스템은 식물로부터 감미단백질 생산을 위한 좋은 대안이 될 수 있을 것으로 생각된다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.355-355
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• 2017

South Korea is largely dependent on imports of forage crops. In order to raise the self-sufficiency rate of forage crops, it is necessary to cultivate forage crops in 135,100 ha reclaimed land of South Korea. This study was conducted to investigate the growth of halophytes and forage crops in order to search for stable plants on reclaimed land. The plants were cultured in Hoagland solution and grown at 0%, 0.5%, 1.0% and 2.0% of salt concentrations. Plants with good growth at 0.5% of salt concentration were halophytes such as Suaeda asparagoides and Salsola komarovii. Salicornia herbacea and Suaeda maritima showed good growth at 2.0% salinity, and Suaeda maritima showed stable growth at all of salt concentrations. In conclusion, Suaeda asparagoides showed the best growth at 0.5% of salt concentration and was considered the most biomass at reclaimed land in Korea with less than 0.5% of salt concentration.

• 산업경영시스템학회지
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• 제24권69호
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• pp.13-22
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• 2001

The efficient quality assurance system to prevent mixed assembly in introduced in this paper, It supports JIT(Just-In-Time) based Kanban and demand pull system. A new system is designed for the synchronized production and quality assurance using barcode and production kanban card, And the performance improvement method for the stable and fast response of the proposed system is suggested in this paper also. As the result of the proposed system, we improved production status visibility and quality traceability.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.6-6
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• 2022

Prolonged Russia-Ukraine war, and unstable situation of supply and demand of global crops including the COVID-19 pandemic have raised awareness regarding food crisis, and in addition to this situation, export restriction measures imposed by some countries have accelerated the rise in the prices. Since the Republic of Korea depends annual crop consumption (21.32 million tons) mostly on the imports (food self-sufficiency rate in 2020 was 45.8%, crop self-sufficiency rate was 20.2%), our main task is to stably secure food. Now we need to put focus on building capacity to secure stable food supply, and actively manage and respond to risks. To overcome this condition, the Korean government set robust food sovereignty as its policy task, and has been focusing on the policy capacity by providing financial and policy support in parallel. We need to implement mid- to long-term measures to strengthen food security as well as to ensure domestic price stability. While increasing the domestic capacity to supply food in the mid- to long-term perspective, we are implementing projects to bring in crops which are inevitable to be imported by private companies. Specifically, we are making efforts to expand infrastructure for the public reserve and domestic production of wheat and beans which have low self-sufficiency rate, and to secure food sovereignty by providing support to secure global crop supply chain to private companies. The Ministry of Agriculture, Food and Rural Affairs plans to set a target for food self-sufficiency rate and prepare a policy to strengthen mid- to long-term food security by establishing a task force to strengthen mid- to long-term food security in the Ministry. Especially, although wheat is the second staple food, domestic wheat production and the foundation for the industry is poor. Compared to the wheat imports, domestic production of wheat is 30 thousand tons (self-sufficiency rate of 1%), leading to a vulnerable status against internal and external shocks. Through the establishment of the Wheat Industry Promotion Act (Feb. 2020) and the First Master Plan for Wheat Industry Promotion (Nov. 2020), the Korean government has developed a policy basis, and has been providing financial support in overall across the production, distribution and consumption process. In addition, the government established a production complex for Korean wheat and beans in order to supply affordable government-supplied commodities, provide education and consulting services, and create a high-quality stable production system, including facilities and equipment. We are also continuing to increase the public reserve for wheat and beans with the purpose of stable supply and demand as well as food security. The Korean government will establish and implement mid- to long-term measures to strengthen the foundation for domestic production across production, distribution and consumption process, and to stably secure global supply chain including through diversified import channels.

• Journal of Microbiology and Biotechnology
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• 제10권1호
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• pp.115-118
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• 2000

Recombinant plasmids harboring a heterologous gene coding for the enhanced green fluorescent protein (EGFP) were transfected and expressed in Drosophila melanogaster S2 cells. A stable transformation of polyclonal cell populations expressing EGFP were isolated after 4 weeks of selection with hygromycin B. The recombinant EFGP expressed in transformed S2 cells consisted of a molecular weight of 27 kDa. EGFP expression was also confirmed by fluorometric measurement. The maximum EGFP concentration was about 9.3 mg/I. The present findings demonstrate not only the successful stable expression of EGFP in Drosophuila was about 9.3 mgI. The present findings demonstrate not only the successful stable expression of EGFP in Drosophila S2 cells, but also the use of EGFP as a reporter to analyze gene expression, with its potential of a Drosophila cell expression system for recombinant protein production being an alternative to a baculovirus-insect cell expression system.

• 대한수의학회지
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• 제28권1호
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• pp.59-65
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• 1988

This paper deals with the 0 groups of citrate utilizing variants of Escherichia coli ($Cit^+$ E. coli) isolated from cattle, the production of colicin, hemolysin, K99 antigen, heat stable enterotoxin, and the isolation of plasmid DNA. Among 42 $Cit^+$ E. coli, 12 strains were 020, 9 strains 08, 5 strains 045, 3 strains 0115, 1 strain 064, 1 strain 0139 and remaining strains(11) were untypable. Thirty-nine(81.3%) out of 48 $Cit^+$ E. coli were produced colicin and 13(27.0%) were produced hemolysin. Of 12 $Cit^+$ E. coli bearing K99 antigen, 6(50.0%) were produced heat stable enterotoxin. In gel electrophoresis for the isolation of plasmid DNA, the number of plasmids varied from 1 to 7 in 10 $Cit^+$ E. coli. It's molecular weight ranged from 2 to 50 Mdalton, and 50 Mdalton plasmid was commonly existed in all strains.

• Journal of Plant Biotechnology
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• 제34권2호
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• pp.95-101
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• 2007

The transport biofuel is emerging a promising option to realize the sustainable growth of our society. Two biofuels, bioethanol and biodiesel, are currently used in the transport sector. As the production of biofuels is getting activated, the stable supply of the feedstocks is becoming a critical issue. Active works have been carried out to secure the stable supply of the raw materials for the production of biofuels. One approach is the breeding of the energy crops to get higher productivity and / or the desirable fuel properties. The other approach is finding new energy crops which may not be used for edible purpose. First current aspects and challenging issues for the implementation of biofuels have been introduced. Finally the recent works and future prospects on the development of the energy crops are summarized.

• Asian-Australasian Journal of Animal Sciences
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• 제29권8호
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• pp.1152-1158
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• 2016

Morphological and physiological alterations occur in the digestive system of weanling piglets, compromising the performance in subsequent phases. This experiment aimed at verifying the influence of glutamine, glutamate and nucleotides on the carbon turnover in the pancreas and liver of piglets weaned at 21 days of age. Four diets were evaluated: glutamine, glutamic acid or nucleotides-free diet (CD); containing 1% glutamine (GD); containing 1% glutamic acid (GAD) and containing 1% nucleotides (ND). One hundred and twenty-three piglets were utilized with three pigs slaughtered at day zero (weaning day) and three at each one of the experimental days (1, 2, 4, 5, 7, 9, 13, 20, 27, and 49 post-weaning), in order to collect organ samples, which were analyzed for the ${\delta}^{13}C$ isotopic composition and compared by means of time. No differences were found (p>0.05) among treatments for the turnover of the $^{13}C$ in the pancreas ($T_{50%}$ = 13.91, 14.37, 11.07, and 9.34 days; $T_{95%}$ = 46.22, 47.73, 36.79, and 31.04 days for CD, GD, GAD, and ND, respectively). In the liver, the ND presented accelerated values of carbon turnover ($T_{50%}=7.36$ and $T_{95%}=24.47days$) in relation to the values obtained for the GD ($T_{50%}=10.15$ and $T_{95%}=33.74days$). However, the values obtained for the CD ($T_{50%}=9.12$ and $T_{95%}=30.31days$) and GAD ($T_{50%}=7.83$ and $T_{95%}=26.03days$) had no differences (p>0.05) among other diets. The technique of $^{13}C$ isotopic dilution demonstrated trophic action of nucleotides in the liver.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.102-102
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• 2022

More than 74% of forage crops in Korea are cultivated in winter rice fields. Italian ryegrass (IRG) can be said to be a representative forage crop, but over 70% of its seeds are dependent on imports. In addition, there are concerns that the stable supply of research fees may be disrupted due to the effects of climate change, war, and other factors. Therefore, it is necessary to secure a large production area for stable supply. Korea's reclaimed land area is 135,000 ha and its agricultural area is 112,000 ha. Therefore, this study attempted to apply IRG to the domestic IRG seed industry through stable cultivation techniques on reclaimed land. In this study, IRG 'Kowiearly' varsity was cultivated in late October 2020 and early October 2021 in the Saemangeum reclaimed land. The seeding methods were conventional drill sowing seeding, new technology spot seedling (30 × 18 cm) and new technology drill sowing seeding. The amount of sowing was conventional drill sowing seeding 2.0(kg/10a), new technology spot seedling 1.5(kg/10a), and new technology drill sowing seeding 1.5(kg/10a). Fertilizer application amount is conventional drill sowing seeding(N-P₂O₅-K₂O) 9.0-12-12(kg/10a), and new technology spot seedling and drill sowing seeding were(N-P₂O₅-K₂O) 4.5-12-12(kg/10a) respectively. Fertilizer was applied accordingly. After that, in February, the conventional drill sowing seeding, new technology drill sowing seeding and spot seedling applied 4.0 and 2.2(kg/10a) of nitrogen supplement fertilizer, respectively. Before wintering, plant length was higher in 2022 than in 2021, but leaf number was higher in 2021. Heading time was April 30, 2021 and April 25, 2022. In heading time, plant length was 74 cm in 2021 and 67 cm in 2022, lower than in 2021. On the other hand, There was no difference in the number of panicle and the number of seeds in the 2021 harvester in all treatment plots, and, thus seed yield was no differ. However, the drill sowing seeding and spot seedling of the new technology were somewhat higher than the conventional drill sowing seeding. On the other hand, seed yield was decreased in all treatment plots compared to 2022 because of raifall deficiency in 2021.

• The International Journal of Costume Culture
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• 제3권3호
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• pp.235-245
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• 2000

The purposes of this study were 1) to investigate usage level of production strategies based on group of production environment, 2) to investigate usage level of production systems based on group of production strategy, and 3) to analyze each of company performance based on group of production strategy and system. For this study, the questionnaires were administered to 215 apparel manufactures in metropolitan area from Feb. to Mar. 1998. Employing a sample of 201, data were analyzed by factor analysis, descriptive statistics, cluster analysis, discriminant analysis, and multivariate analysis of variance. The following are the results of this study. 1. Concerning production strategy due to group of production environment, the stable group and the complicated group prefer to rice/quality centered strategy but the level of usage for strategies is so pretty that it is not significant to carry out them. 2. Concerning production system due to group of production strategy, the workers centered group is occupied high in the price/quality centered group & the complex group. And also the product centered system is occupied high in the flexibility centered group. 3. Concerning company performance due to group of production strategy and system, the price/quality centered group holds low position of performance comparing to another groups. And the performance of the managers centered group is higher than that of the workers.