• Molecules and Cells
• /
• 제19권2호
• /
• pp.239-245
• /
• 2005

Factor for inversion stimulation (FIS), the Escherichia coli protein, is a positive regulator of the transcription of genes that encode stable RNA species, such as rRNA and tRNA. Transcription of the rnpB gene encoding M1 RNA, the catalytic subunit of E. coli RNase P, rapidly declines under stringent conditions, as does that of other stable RNAs. There are multiple putative FIS binding sites upstream of the rnpB promoter. We tested whether FIS binds to these sites, and if so, how it affects rnpB transcription. In vitro binding assays revealed specific binding of FIS to multiple sites in the rnpB promoter region. Interestingly, FIS bound not only to the upstream region of the promoter, but also to the region from +4 to +18. FIS activated rnpB transcription in vitro, but the level of activation was much lower than that of the rrnB promoter for rRNA. We also examined the effects of FIS on rnpB transcription in vivo using isogenic $fis^+$ and $fis^-$ strains. rnpB transcription was higher in the $fis^-$ than the $fis^+$ cells during the transitions from lag to exponential phase, and from exponential to stationary phase.

• 대한기계학회논문집B
• /
• 제26권10호
• /
• pp.1458-1464
• /
• 2002

$CO_2$ is well known greenhouse gas which is the major source of global warming. Reducing $CO_2$ emission in combustion process can be achieved by increasing combustion efficiency, oxygen enriched combustion and recirculation of the emitted $CO_2$ gas. Stability of non-premixed flame in oxygen enriched environment will be affected by the amount of oxygen, kind of diluents and fuel exit velocity. The effects of these parameters on flame liftoff and blowout are studied experimentally oxidizer coflowing burner. Experiments were divided into three cases according as where $CO_2$gas was supplied. - 1) to coflowing air, 2) to fuel with 0$_2$-$N_2$ coflow, 3) to coflowing oxygen. Flame in air coflowing case was lifted in turbulent region. Flame lift and blowout in laminar region with the increase in $CO_2$ volume fraction in $CO_2$-Air mixture makes flame lift and blowout in laminar region. Increase in oxygen volume fraction makes flame stable-i.e. flame liftoff and blowout occur at higher fuel flowrates. Liftoff height was non-linear function of nozzle exit velocity and affected by the $O_2$ volume fraction. It was found that the flame in $O_2$-$N_2$ coflow case was more stable than $O_2$-$CO_2$ case, Liftoff heights vs (nozzle exit velocity/laminar burning velocity)$^{3.8}$ has a good correlation in $O_2$-$CO_2$ oxidizer case.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 1998년도 추계학술대회 논문집 학회본부 C
• /
• pp.795-797
• /
• 1998

A $BaTiO_3$, ferroelectric material, was mixed $SrTiO_3$, $(x)BaTiO_3-(1-x)SrTiO_3$($0.7{\leq}x{\leq}1$) ceramic capacitor with stable electrical properties in high voltage was fabricated. And microstructure, electrical property were investigated with $SrTiO_3$ mol ratio. The shrinkage, open porosity, sintering density were predominated at $9BaTiO_3-0.1SrTiO_3$. Increasing $SrTiO_3$ mol ratio, curie temperature was shifted at low temperature and maximum permittivity was increased. Also, $0.9BaTiO_3-0.1SrTiO_3$ was showed stable dielectric properties at $25{\sim}80[^{\circ}C]$. V-I properties of specimen were observed in the temperature range of $21{\sim}143[^{\circ}C]$, were divided into three regions. The region I below 10[kV/cm] was shown Ohmic conduction, the region II from 10 to 30[kV/cm] was explained by the Poole-Frenkel emission theory and the region III above 30[kV/cm] was analysed by the tunneling effect.

• 한국컴퓨터정보학회논문지
• /
• 제19권8호
• /
• pp.35-44
• /
• 2014

본 논문에서는 효율적인 핸드 마우스를 위한 3D 포인팅 인터페이스에 대해 제안한다. 제안하는 방법에서는 조명과 환경 변화에 강건하기 위해 깊이영상을 이용하고, 손바닥의 법선벡터를 이용하여 3D 포인팅을 구성한다. 먼저, 손 영역 검출과 추적은 기존 방법을 이용하고, 이로부터 획득한 정보를 바탕으로 손바닥의 영역을 예측하여 관심 영역을 획득한다. 관심 영역을 획득하면, 해당 영역을 평면의 방정식으로 근사시키고, 법선 벡터를 추출한다. 다음으로, 안정적인 제어를 위해 추출한 법선 벡터를 이용하여 보간을 수행하고, 교점을 검출한다. 검출된 교점은 안정성과 효율성을 위해 시그모이드 함수를 이용한 동적 가중치가 적용되고, 최종적으로 2D 좌표계로 변환된다. 본 논문에서는 관심 영역, 방향 벡터 검출 방법에 대해 설명하고 안정적인 제어를 위한 보간 방법과 동적 가중치 적용방법에 대해 제안한다. 마지막으로 제안된 3D 포인팅의 정성적, 정량적 분석을 통해 안정적인 제어 가능성을 입증한다.

• 대한기계학회논문집B
• /
• 제21권4호
• /
• pp.473-485
• /
• 1997

Detailed experimental study has been made of air blast kerosene spray flames with and without swirl in combustion air flow. Phase-Doppler detect technique is used to measure Sauter mean diameter, axial component mean and rms velocity, size-velocity correlation, and number density. These measurements are obtained for both nonreacting and reacting cases under several stable flame conditions. The results show that the introduction of swirl to the combustion air modifies the spatial distribution of droplet size, velocity, and number density, and thus alters the flame structure. However, due to the weak swirl intensity, the overall structure of swirling flames are essentially same as that of nonswirling flames. Physical model of structure of air blast atomized spray flames is projected to show that spray flames are composed of three distinct regions: the two-phase mixture region, the main reaction and the intermittent combustion region. Near the atomizer, two phase mixture of droplet and air is formed in the core region. This dense spray region is characterized by high droplet number density and the strong convective effect. There follows the main combustion region where the main flame penetrates within the spray boundary. Main reaction region of these flames are governed by internal group combustion mode. Finally there exists the intermittent combustion region where local group burning or isolated droplet burning occurs.

• 융합신호처리학회논문지
• /
• 제10권4호
• /
• pp.233-238
• /
• 2009

본 논문에서는 얼굴을 자동 초점(Auto-focus) 디지털 카메라의 관심영역(ROI : Region Of Interest)으로 이용하는 시스템에서 요구되는 검출 영역의 안정적인 선택을 위한 방법을 제안한다. 이 방법은 디지털 카메라와 모바일 카메라에 포함되는 ISP(Image Signal Processor)에서 실시간으로 처리되는 프로그레시브 입력 영상에서 얼굴 영역을 관심영역으로 간주하고 자동으로 초점을 맞추는 방법이다. 얼굴 영역 검출을 위하여 사용한 학습 알고리즘은 에이다부스트 알고리즘을 이용하였다. 학습에 포함되지 않은 기울어진 얼굴에 대한 검출방법과 검출 결과에 대한 후처리 방법, 관심영역이 흔들리지 않고 일정한 영역을 유지하도록 하기 위한 안정화 대책을 제안한다. 제안된 ROI 영역 안정화 알고리즘에 대한 성능을 평가하기 위하여 움직임이 있는 얼굴에 대하여 기준 궤적과의 차이를 보이고, 각 궤적의 회귀곡선과의 RMS 오차를 안정화 성능평가의 척도로 이용하였다.

• BMB Reports
• /
• 제39권1호
• /
• pp.61-67
• /
• 2006

Molecular co-suppression phenomena are important to consider in transgene experiments. Embryogenic cells were obtained from immature cotyledons and engineered with two different gene constructs (pHV and pHVS) through particle bombardment. Both constructs contain a gene conferring resistance to hygromycin (hpt) as a selective marker and a modified glycinin (11S globulin) gene (V3-1) as a target. sGFP(S65T) as a reporter gene was, however, inserted into the flanking region of the V3-1 gene (pHVS). Fluorescence microscopic screening after the selection of hygromycin, identified clearly the expression of sGFP(S65T) in the transformed soybean embryos bombarded with the pHVS construct. Stable integration of the transgenes was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. Seeds of transgenic plants obtained from the pHV construct frequently lacked an accumulation of endogenous glycinin, which is encoded by homologous genes to the target gene V3-1. Most of the transgenic plants expressing sGFP(S65T) showed highly accumulation of glycinin. The expression of sGFP(S65T) and V3-1 inherits into the next generations. sGFP(S65T) as a reporter gene may be useful to increase the transformation efficiency of transgenic soybean with avoiding gene co-suppression.

• 한국연소학회:학술대회논문집
• /
• 한국연소학회 2004년도 제28회 KOSCO SYMPOSIUM 논문집
• /
• pp.42-48
• /
• 2004

Lean laminar premixed propane and methane flames which were anchored by a hydrogen-pilot flame in a tube were investigated experimentally. The flame shapes were observed by varying mean velocity from 10cm/s to 140cm/s and equivalence ratio from 0.45 to 0.8. In this study, behaviors of flames are divided into five regions such as tail-out, flash-back, flickering, stable and vibrating flames with respect to the mean velocity and the equivalence ratio. Although the flames are unstable in both the flickering and the vibrating region, they have different characteristics such as the frequency, sound generation and creation process of flame curvature. The flickering region exists near the flammability limit and the flame flickers in a frequency of about 10Hz. When flame front is bended, the propane flame front is straightened and the methane flame front is bended more by thermo-diffusive instability. In the vibrating region, the flame vibrates emitting audible sound in a frequency of about 100Hz. In the boundary of vibrating region, the vibration of flame changes between two modes such as single frequency vibration and dual frequency vibration. Increase and decrease of vibration in each mode are determined by thermo-acoustic instability.

• 한국전기전자재료학회:학술대회논문집
• /
• 한국전기전자재료학회 1997년도 춘계학술대회 논문집
• /
• pp.318-322
• /
• 1997

The (Sr$_{l-x}$.Ca$_{x}$)TiO$_3$(0.05$\leq$x$\leq$0.2) ceramics were fabricated to form semiconducting ceramics by sintering at about 1350[$^{\circ}C$] in a reducing atmosphere($N_2$gas). After being fired in a reducing atmosphere, metal oxides, CuO, was painted on the both surface of the specimens to diffuse to the grain boundary. They were annealed at 1100[$^{\circ}C$] for 2 hours and cooled to room temperature. The grain boundary was composed of the continuous insulating layers. The capacitance changes slowly and almost linearly in the temperature region of -30~+85[$^{\circ}C$]. The capacitance characteristics appears a stable value within $\pm$10[%]. The conduction mechanism of the specimens observed in the temperature range of 25~125[$^{\circ}C$], and is divined into three regions haying different mechanism as the current increased: the region I below 230[V/cm] shows the ohmic conduction. The region II can be explained by the Poole-Frenkel emission theory, and the region III is dominated by the tunneling effect.ect.

• 한국세라믹학회지
• /
• 제31권12호
• /
• pp.1491-1500
• /
• 1994

Nonstoichiometry($\delta$) and the phase stability region of a ferrite spinel (Mg0.29Fe0.71)3-$\delta$O4 have been investigated by a coulometric titration method as a function of temperature(T) and oxygen partial pressure(Po2). It has been found that the spinel is thermodynamically stable in the ranges -8.0$\leq$log(PO2/atm)$\leq$-2.4, -7.0$\leq$log(PO2/atm)$\leq$-1.7 respectvely at 100$0^{\circ}C$. The nonstoichiometry extends over the ranges of -0.004$\leq$$\delta$$\leq$0.007, -0.008$\leq$$\delta$$\leq$0.006, -0.033$\leq$$\delta$$\leq$0.004 at 100$0^{\circ}C$, 120$0^{\circ}C$, respectvely. The observed PO2-dependence of $\delta$ suggests that the majority ionic defects are cation interstitials in the low PO2 region and cation vacancies in the high PO2 region.