• Molecules and Cells
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• v.19 no.2
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• pp.239-245
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• 2005

Factor for inversion stimulation (FIS), the Escherichia coli protein, is a positive regulator of the transcription of genes that encode stable RNA species, such as rRNA and tRNA. Transcription of the rnpB gene encoding M1 RNA, the catalytic subunit of E. coli RNase P, rapidly declines under stringent conditions, as does that of other stable RNAs. There are multiple putative FIS binding sites upstream of the rnpB promoter. We tested whether FIS binds to these sites, and if so, how it affects rnpB transcription. In vitro binding assays revealed specific binding of FIS to multiple sites in the rnpB promoter region. Interestingly, FIS bound not only to the upstream region of the promoter, but also to the region from +4 to +18. FIS activated rnpB transcription in vitro, but the level of activation was much lower than that of the rrnB promoter for rRNA. We also examined the effects of FIS on rnpB transcription in vivo using isogenic $fis^+$ and $fis^-$ strains. rnpB transcription was higher in the $fis^-$ than the $fis^+$ cells during the transitions from lag to exponential phase, and from exponential to stationary phase.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.10
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• pp.1458-1464
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• 2002

$CO_2$ is well known greenhouse gas which is the major source of global warming. Reducing $CO_2$ emission in combustion process can be achieved by increasing combustion efficiency, oxygen enriched combustion and recirculation of the emitted $CO_2$ gas. Stability of non-premixed flame in oxygen enriched environment will be affected by the amount of oxygen, kind of diluents and fuel exit velocity. The effects of these parameters on flame liftoff and blowout are studied experimentally oxidizer coflowing burner. Experiments were divided into three cases according as where $CO_2$gas was supplied. - 1) to coflowing air, 2) to fuel with 0$_2$-$N_2$ coflow, 3) to coflowing oxygen. Flame in air coflowing case was lifted in turbulent region. Flame lift and blowout in laminar region with the increase in $CO_2$ volume fraction in $CO_2$-Air mixture makes flame lift and blowout in laminar region. Increase in oxygen volume fraction makes flame stable-i.e. flame liftoff and blowout occur at higher fuel flowrates. Liftoff height was non-linear function of nozzle exit velocity and affected by the $O_2$ volume fraction. It was found that the flame in $O_2$-$N_2$ coflow case was more stable than $O_2$-$CO_2$ case, Liftoff heights vs (nozzle exit velocity/laminar burning velocity)$^{3.8}$ has a good correlation in $O_2$-$CO_2$ oxidizer case.

• Proceedings of the KIEE Conference
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• 1998.11c
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• pp.795-797
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• 1998

A $BaTiO_3$, ferroelectric material, was mixed $SrTiO_3$, $(x)BaTiO_3-(1-x)SrTiO_3$($0.7{\leq}x{\leq}1$) ceramic capacitor with stable electrical properties in high voltage was fabricated. And microstructure, electrical property were investigated with $SrTiO_3$ mol ratio. The shrinkage, open porosity, sintering density were predominated at $9BaTiO_3-0.1SrTiO_3$. Increasing $SrTiO_3$ mol ratio, curie temperature was shifted at low temperature and maximum permittivity was increased. Also, $0.9BaTiO_3-0.1SrTiO_3$ was showed stable dielectric properties at $25{\sim}80[^{\circ}C]$. V-I properties of specimen were observed in the temperature range of $21{\sim}143[^{\circ}C]$, were divided into three regions. The region I below 10[kV/cm] was shown Ohmic conduction, the region II from 10 to 30[kV/cm] was explained by the Poole-Frenkel emission theory and the region III above 30[kV/cm] was analysed by the tunneling effect.

• Journal of the Korea Society of Computer and Information
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• v.19 no.8
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• pp.35-44
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• 2014

This paper proposes a 3D pointing interface that is designed for the efficient application of a hand mouse. The proposed method uses depth images to secure high-quality results even in response to changes in lighting and environmental conditions and uses the normal vector of the palm of the hand to perform 3D pointing. First, the hand region is detected and tracked using the existing conventional method; based on the information thus obtained, the region of the palm is predicted and the region of interest is obtained. Once the region of interest has been identified, this region is approximated by the plane equation and the normal vector is extracted. Next, to ensure stable control, interpolation is performed using the extracted normal vector and the intersection point is detected. For stability and efficiency, the dynamic weight using the sigmoid function is applied to the above detected intersection point, and finally, this is converted into the 2D coordinate system. This paper explains the methods of detecting the region of interest and the direction vector and proposes a method of interpolating and applying the dynamic weight in order to stabilize control. Lastly, qualitative and quantitative analyses are performed on the proposed 3D pointing method to verify its ability to deliver stable control.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.21 no.4
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• pp.473-485
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• 1997

Detailed experimental study has been made of air blast kerosene spray flames with and without swirl in combustion air flow. Phase-Doppler detect technique is used to measure Sauter mean diameter, axial component mean and rms velocity, size-velocity correlation, and number density. These measurements are obtained for both nonreacting and reacting cases under several stable flame conditions. The results show that the introduction of swirl to the combustion air modifies the spatial distribution of droplet size, velocity, and number density, and thus alters the flame structure. However, due to the weak swirl intensity, the overall structure of swirling flames are essentially same as that of nonswirling flames. Physical model of structure of air blast atomized spray flames is projected to show that spray flames are composed of three distinct regions: the two-phase mixture region, the main reaction and the intermittent combustion region. Near the atomizer, two phase mixture of droplet and air is formed in the core region. This dense spray region is characterized by high droplet number density and the strong convective effect. There follows the main combustion region where the main flame penetrates within the spray boundary. Main reaction region of these flames are governed by internal group combustion mode. Finally there exists the intermittent combustion region where local group burning or isolated droplet burning occurs.

• Journal of the Institute of Convergence Signal Processing
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• v.10 no.4
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• pp.233-238
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• 2009

In this paper, we propose the methods to select the stable region for the detect region which is required in the system used the face to the ROI in the auto-focus digital camera. this method regards the face region as the ROI in the progressive input frame and focusing the region in the mobile camera embeded ISP module automatically. The learning algorithm to detect the face is the Adaboost algorithm. we proposed the method to detect the slanted face not participate in the train process and postprocessing method for the results of detection, and then we proposed the stabilization method to sustain the region not shake for the region. we estimated the capability for the stabilization algorithm using the RMS between the trajectory and regression curve.

• BMB Reports
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• v.39 no.1
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• pp.61-67
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• 2006

Molecular co-suppression phenomena are important to consider in transgene experiments. Embryogenic cells were obtained from immature cotyledons and engineered with two different gene constructs (pHV and pHVS) through particle bombardment. Both constructs contain a gene conferring resistance to hygromycin (hpt) as a selective marker and a modified glycinin (11S globulin) gene (V3-1) as a target. sGFP(S65T) as a reporter gene was, however, inserted into the flanking region of the V3-1 gene (pHVS). Fluorescence microscopic screening after the selection of hygromycin, identified clearly the expression of sGFP(S65T) in the transformed soybean embryos bombarded with the pHVS construct. Stable integration of the transgenes was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. Seeds of transgenic plants obtained from the pHV construct frequently lacked an accumulation of endogenous glycinin, which is encoded by homologous genes to the target gene V3-1. Most of the transgenic plants expressing sGFP(S65T) showed highly accumulation of glycinin. The expression of sGFP(S65T) and V3-1 inherits into the next generations. sGFP(S65T) as a reporter gene may be useful to increase the transformation efficiency of transgenic soybean with avoiding gene co-suppression.

• 한국연소학회:학술대회논문집
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• 2004.06a
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• pp.42-48
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• 2004

Lean laminar premixed propane and methane flames which were anchored by a hydrogen-pilot flame in a tube were investigated experimentally. The flame shapes were observed by varying mean velocity from 10cm/s to 140cm/s and equivalence ratio from 0.45 to 0.8. In this study, behaviors of flames are divided into five regions such as tail-out, flash-back, flickering, stable and vibrating flames with respect to the mean velocity and the equivalence ratio. Although the flames are unstable in both the flickering and the vibrating region, they have different characteristics such as the frequency, sound generation and creation process of flame curvature. The flickering region exists near the flammability limit and the flame flickers in a frequency of about 10Hz. When flame front is bended, the propane flame front is straightened and the methane flame front is bended more by thermo-diffusive instability. In the vibrating region, the flame vibrates emitting audible sound in a frequency of about 100Hz. In the boundary of vibrating region, the vibration of flame changes between two modes such as single frequency vibration and dual frequency vibration. Increase and decrease of vibration in each mode are determined by thermo-acoustic instability.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 1997.04a
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• pp.318-322
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• 1997

The (Sr$_{l-x}$.Ca$_{x}$)TiO$_3$(0.05$\leq$x$\leq$0.2) ceramics were fabricated to form semiconducting ceramics by sintering at about 1350[$^{\circ}C$] in a reducing atmosphere($N_2$gas). After being fired in a reducing atmosphere, metal oxides, CuO, was painted on the both surface of the specimens to diffuse to the grain boundary. They were annealed at 1100[$^{\circ}C$] for 2 hours and cooled to room temperature. The grain boundary was composed of the continuous insulating layers. The capacitance changes slowly and almost linearly in the temperature region of -30~+85[$^{\circ}C$]. The capacitance characteristics appears a stable value within $\pm$10[%]. The conduction mechanism of the specimens observed in the temperature range of 25~125[$^{\circ}C$], and is divined into three regions haying different mechanism as the current increased: the region I below 230[V/cm] shows the ohmic conduction. The region II can be explained by the Poole-Frenkel emission theory, and the region III is dominated by the tunneling effect.ect.

• Journal of the Korean Ceramic Society
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• v.31 no.12
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• pp.1491-1500
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• 1994

Nonstoichiometry($\delta$) and the phase stability region of a ferrite spinel (Mg0.29Fe0.71)3-$\delta$O4 have been investigated by a coulometric titration method as a function of temperature(T) and oxygen partial pressure(Po2). It has been found that the spinel is thermodynamically stable in the ranges -8.0$\leq$log(PO2/atm)$\leq$-2.4, -7.0$\leq$log(PO2/atm)$\leq$-1.7 respectvely at 100$0^{\circ}C$. The nonstoichiometry extends over the ranges of -0.004$\leq$$\delta$$\leq$0.007, -0.008$\leq$$\delta$$\leq$0.006, -0.033$\leq$$\delta$$\leq$0.004 at 100$0^{\circ}C$, 120$0^{\circ}C$, respectvely. The observed PO2-dependence of $\delta$ suggests that the majority ionic defects are cation interstitials in the low PO2 region and cation vacancies in the high PO2 region.