• Animal cells and systems
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• 제6권3호
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• pp.227-232
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• 2002

GroESLx proteins of symbiotic X-bacteria were overproduced in Escherichia coli and their structural characteristics were assayed after simple purification. The GroESx and GroELx were heat-stable at 8$0^{\circ}C$ and 5$0^{\circ}C$, respectively. After heat-treatment, GroESx was purified by DEAE Sephadex A-50 chromatography and GroELx was purified by step- and linear sucrose density gradient ultracentrifugation. Molecular masses of GroESx and GroELx were 50-80 kDa and 800 kDa, respectively, as estimated by sucrose density gradient ultracentrifugation. In chemical cross-linking analysis, subunits of GroESx were mostly cross-linked by incubation for 3 h in 0.4% glutaralde-hyde and GroESx was found to be composed of homo-heptamer subunits. Those of GroELx were cross-linked within 10 min in 0.3% glutaraldehyde and GroELx was in two stacks of homo-heptamer subunits. On the other hand, GroESx and GroELx proteins in a solution could not be cross-linked even after incubation for 3 h in 0.5% glutaraldehyde. GroELx was stable at 4-37$^{\circ}C$. In the presence of both GroESx and ATP, GroELx$_{14}$ was stable at 37$^{\circ}C$ but not at 4$^{\circ}C$ or 24$^{\circ}C$. Thus, we confirmed the oligomeric properties of GroESx$_{7}$ and GroELx$_{14}$ and their stability to heat and in the interaction with GroESx.x.

• Composites Research
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• 제18권4호
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• pp.14-20
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• 2005

방향족아민 경화제의 벤젠링 사이의 관능기 변화가 반응특성, 기계적특성 및 열적특성에 미치는 영향을 고찰하기 위하여 범용 에폭시수지인 DGEBF와 방향족아민 경화제 DDM, DDS 각각을 1:1의 당량비로 혼합하여 동일한 조건으로 경화하였다. 그 결과 에폭시수지 경화물의 특성은 경화제의 벤젠링 사이의 관능기 구조에 따라 매우 큰 영향을 받았다. 즉, 벤젠링 사이에 길이가 길고 bulky한 술폰기를 포함한 DDS는 반응성, 열안정성, 밀도, shrinkage($\%$), 선팽창계수, 인장탄성율. 인장강도, 굴곡탄성율, 굴곡강도의 값이 벤젠링 사이에 메틸기를 가지는 DDM 경화물계 보다 높게 나타났지만, epoxide기의 전환율, 유리전이온도의 값은 낮게 나타냈다. 이것은 술폰기의 강한 전기음성도와 메틸기의 전기 양성적인 repulsive한 성질의 차이에 의한 영향인 것으로 판단된다. 굴곡 파단면의 관찰 결과는 DDS 경화물계의 각개 grain이 불규칙적으로 분포되어 있음을 확연하게 보여준다.

• 전기학회논문지
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• 제62권10호
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• pp.1383-1389
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• 2013

There were telecommunication noise and malfunctions of the electronic devices occurred over a wide area due to the high harmonic voltage and/or current levels of the Back-to-back converter in the DFIG wind power system even though the magnitude of all harmonics is within the international standards. The triangular carrier signals of the PWM used in the power converter system is related to the telecommunication noise because they are in the range of audible frequencies and amplified by a variety of the standing waves that were excited by harmonic voltage sources in the weak grid system such as a long distance distribution transmission lines. This paper describes the characteristics of the harmonics in the wind turbine-generator, numerical analysis and simulation of the harmonics resonance phenomena in the distribution lines as well as measuring induced voltage of the telecommunication lines in parallel with power lines in order to verify the root cause of the telecommunication noise. These noise problems can occur in a wind turbine power system with a non-linear converter at any time, as well as photovoltaic power system. So, the preliminary review of suitable filter devices and switching frequencies of the PWM have to be required by considering the stability of the controller at the design stage but as part of the measures the effect of the telecommunication cable shields was analyzed by comparing the measured data between multi-conductor with/without shields so as to attenuate the sources of the harmonics voltage induced into the telecommunication lines and to apply the most cost-effective measures in the field.

• 한국강구조학회 논문집
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• 제24권6호
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• pp.683-692
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• 2012

사장교의 거더에는 케이블의 수평방향 분력에 의하여 휨모멘트 외에 부가적인 압축력이 가해지게 된다. 세장하게 설계되는 거더는 이러한 압축력에 의해 강성이 저하될 수 있으며, FCM으로 가설되는 사장교의 경우 이러한 문제는 완성계보다 시공중 모델에 대하여 고려되어야 할 필요가 있다. 현행 설계기준에서는 사장교 거더의 안정성 평가를 위하여 선형탄성고유치 해석법을 제안하고 있다. 그러나 이러한 방법으로는 비선형성 거동을 하는 사장교의 구조적 특성을 반영할 수 없을 뿐 아니라, 완성계 상태를 기준으로 하는 해석 방법은 시공중 모델에 대한 적용이 어렵다 할 수 있다. 따라서 본 연구에서는 FCM으로 가설되는 2주탑 3경간 강사장교에 있어, 케이블 거치 형식과 거더의 강성에 따라 총 3개의 모델을 제작하고 실험적, 해석적으로 시공중 사장교의 거동 특성을 분석하였다.

• Journal of Microbiology and Biotechnology
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• 제23권5호
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• pp.674-680
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• 2013

Yeast Dekkera/Brettanomyces bruxellensis is probably the most common contaminant in wineries and ethanol production processes. The considerable economic losses caused by this yeast, but also its ability to produce and tolerate high ethanol concentrations, make it an attractive subject for research with potential for industrial applications. Unfortunately, efforts to understand the biology of D. bruxellensis and facilitate its broader use in industry are hampered by the lack of adequate procedures for delivery of exogenous DNA into this organism. Here we describe the development of transformation protocols (spheroplast transformation, LiAc/PEG method, and electroporation) and report the first genetic transformation of yeast D. bruxellensis. A linear heterologous DNA fragment carrying the kanMX4 sequence was used for transformation, which allowed transformants to be selected on plates containing geneticin. We found the spheroplast transformation method using 1M sorbitol as osmotic stabilizer to be inappropriate because sorbitol strikingly decreases the plating efficiency of both D. bruxellensis spheroplast and intact cells. However, we managed to modify the LiAc/PEG transformation method and electroporation to accommodate D. bruxellensis transformation, achieving efficiencies of 0.6-16 and 10-20 transformants/${\mu}g$ DNA, respectively. The stability of the transformants ranged from 93.6% to 100%. All putative transformants were analyzed by Southern blot using the kanMX4 sequence as a hybridization probe, which confirmed that the transforming DNA fragment had integrated into the genome. The results of the molecular analysis were consistent with the expected illegitimate integration of a heterologous transforming fragment.

• 한국항해항만학회지
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• 제31권6호
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• pp.523-530
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• 2007

크레인 시스템은 항만 터미널 등의 산업현장에서 무거운 물체를 이송하는데 사용되는 장비로서 그 정확성과 신속성을 동시에 만족시키기 위한 연구가 활발히 진행되고 있다. 본 논문은 적응제어기의 일종인 모델매칭 기법을 이용하여 복잡한 3 자유도 비선형 크레인의 제어 시스템에 대한 연구를 제안한다. 피드백 선형화(feedback linearization)를 통해 비선형 크레인 모델을 선형화한 후 PD 제어기를 적용하여 선형 공칭 모텔을 구한다. 이 모델은 시스템 섭동을 갖는 실시간 시스템 모델과 함께, 리아푸노브(Lyapunov) 이론을 적용하여 실시간 섭동에 의해 발생되는 제어오차를 감소하기 위한 보조 제어규칙의 산출에 이용된다. 또한 리아푸노브 안정성이론을 적용하여 구성한 크레인 제어시스템의 안정성 해석을 실시한다. 컴퓨터 시뮬레이션을 통해 제안한 알고리즘의 타당성을 검증하며 기존의 제어방식과 비교 분석하여 그 우수성을 입증한다.

• 한국강구조학회 논문집
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• 제11권3호통권40호
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• pp.281-290
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• 1999

수십 년 동안, 철도는 차량의 운영과 조작 기술에 맞추어 짧은 길이의 레일을 서로 신축 이음을 두고 연결한 궤도를 이용하여 건설되었다. 그러나 이러한 신축이음들은 궤도 손상과 유지비 증가 등의 많은 단점들을 유발시켰다. 그래서, 철도 기술자들은 차량, 용접, 체결의 기술이 발전함에 따라 열차 하중, 속도의 증가를 위해 레일 사이의 신축 이음을 제거하는 데 많은 관심을 가지게 되었다. 그래서 최근 등장한 것이 기존 레일의 단점들을 해소할 수 있는 CWR궤도이다. 그러나 이러한 CWR궤도는 기존 레일의 단점을 극복할 수 있었으나 레일의 장대화에 따른 온도 하중으로 인한 레일의 좌굴 문제를 야기시켰다. 본 논문에서는 유한 요소 해석을 이용하여 선형 좌굴 해석을 위한 CWR 궤도의 모델과 해석 프로그램인 CWRB를 개발하였다. 레일의 해석 모델은 레일요소 당딤을 고려한 14개의 자유도로 나타내었으며, 체결재, 침목, 도상 저항력을 스프링 요소를 사용하였다. 장대레일의 온도하중에 의한 좌굴 온도 및 모드의 분석으로 장대레일의 온도 좌굴 특성을 고찰하였다.

• Structural Engineering and Mechanics
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• 제64권4호
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• pp.391-402
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• 2017

In this paper, a new nonlocal trigonometric shear deformation theory is proposed for thermal buckling response of nanosize functionally graded (FG) nano-plates resting on two-parameter elastic foundation under various types of thermal environments. This theory uses for the first time, undetermined integral variables and it contains only four unknowns, that is even less than the first shear deformation theory (FSDT). It is considered that the FG nano-plate is exposed to uniform, linear and sinusoidal temperature rises. Mori-Tanaka model is utilized to define the gradually variation of material properties along the plate thickness. Nonlocal elasticity theory of Eringen is employed to capture the size influences. Through the stationary potential energy the governing equations are derived for a refined nonlocal four-variable shear deformation plate theory and then solved analytically. A variety of examples is proposed to demonstrate the importance of elastic foundation parameters, various temperature fields, nonlocality, material composition, aspect and side-to-thickness ratios on critical stability temperatures of FG nano-plate.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.394.2-394.2
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• 2014

We fabricated highly transparent and flexible Ti doped In2O3 (TIO)/Ag nanowire(NW)/TIO (TAT) multilayer electrodes by linear facing target sputtering (LFTS) and brush-painting for used as flexible for anode organic solar cells(FOSCs). The characteristics of TAT transparent anode as a function of number of brush-painting cycles was also investigated. At optimized conditions we achieved highly flexible TAT multilayer electrodes with a low sheet resistance of $9.01{\Omega}/square$ and a high diffusive transmittance more than 80% in visible region as well as superior mechanical stability. The effective embedment of the Ag NW network between top and bottom TIO films led to a metallic conductivity, high transparency. Based on FE-SEM HRTEM, and XRD analysis, we can find that the Ag NW network was effectively embedded between top and bottom TIO layers due to good flexibility of Ag NW, the TAT multilayer showed superior flexibility than single TIO layer. Successful operation of FOSCs with high power conversion efficiency of 3.01% indicates that TAT hybrid electrode is a promising alternative to conventional ITO electrode for high performance FOSCs.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2009년도 춘계학술발표회 논문집
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• pp.23-28
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• 2009

The purpose of this study was to improve not only wet-end performances but also paper characteristics by the modification of various factors like molecular design and ionic characteristics of polyacrylamides First of all physical characteristics were observed after modify molecular design of the cationic polyacrylamides to linear, branched and cross-linked. In addition it was found analysis method to confirm branch degree of cationic polyacrylamides to combine ionic titration characteristics and spectroscopic behavior, After application of these structure modified polyacrylamides to the multiple retention systems with inorganic microparticles, it was found adjusting of branch degree of polyacrylamides was very important to optimize wet-end improvement. Second, After polymerization of amphoteric polyacrylamide to have both of cationic and anionic functional group in the polymer, we observed not only physical characteristics but also wet-end improvement to apply recycled pulp and found that the improvement of solution stability to prevent hydrolysis and increase of ash retention dramatically to compare traditional cationic polyacrylamide retention aid, Finally, After polymerization of anionic polyacrylamide, we observed not only wet-end improvement but also paper characteristics to apply preflocculation of PCC and it was found the improvements of flocculation efficiency, retention, ash retention, optical properties of the paper and bursting strength to compare traditional preflocculant of cationic polyacrylamide.