• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.5
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• pp.413-418
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• 2003

Genetic diversity and soybean sprout-related traits were evaluated in a total of 72 soybean accessions (60 Glycine max, 7 Glycine soja, and 5 Glycine gracilis). 100-seed weight (SW) was greatly varied and ranged from 3.2g to 32.3g in 72 soybean accessions. Positive correlation was observed between GR and hypocotyl length (HL), whereas negative correlation was observed between SW and hypocotyl diameter (HD). Re-evaluation by discarding two soybean genotypes characterized with low GR indicated that much higher correlation of sprout yield (SY) with HD and SW. Based on the principal component analysis (PCA) for sprout-related traits, 57 accessions were classified. Soybean genotypes with better traits for sprout, such as small size of seeds and high SY, were characterized with high PCA 1 and PCA 2 values. The seed size in second is small but showed low GR and SY, whereas the third has large seed, high GR and more than 400% SY. In genetic similarity analysis using 60 SSR marker genotyping, 72 accessions were classified into three major and several minor groups. Nine of twelve accessions that were identified as the representatives of soybean for sprout based on PCA were in a group by the SSR marker analysis, indicating the SSR marker selection of parental genotypes for soybean sprout improvement program.

• The Plant Pathology Journal
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• v.19 no.6
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• pp.280-283
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• 2003

A soybean-sprout rot epidemic occurred in a mass production soybean sprout factory in 2000 and 2001 in Korea, which caused up to 20% production loss. Among the causal pathogenic bacteria and fungi, Pythium deliense was found to be the dominant pathogen of severe root and hypocotyls rot, particularly in recirculating water system. An average of 90% of the isolated fungi from the rotted sprout on potato dextrose agar were Pythium sp. The fungal density of Pythium in the sampled water was monitored in the recycled water system for 1 year using a selective medium (com meal agar with Pimaricin, 10 mg； Rifampicin, 10 mg； and Ampicillin, 100 mg per 1 liter). The drained water from the soybean-sprout cultivation always had a certain amount of fungus in it. The removal of Pythium from the recycling water system must be thorough, safe, and environment friendly. However, the pathogen in the water was easily found even after ozone and chlorine treatments, which were devised on the recycling system for the removal of microorganisms. 5-$\mu\textrm{m}$ pore size filter was applied and was able to successfully control the disease. As the sprout industry increasingly shifts into mass production, the demand for water will increase continuously. Recycling water for sprout production is eco-friendly. However, a process must be devised to be able to first decompose organic matters before Pythium zoospores are filtered.

• Korean Journal of Plant Resources
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• v.12 no.2
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• pp.152-160
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• 1999

Bacterial population density on soybean leaves was $10^2~10^5CFU/cm^2$. Bacterial population density was increased by progress of plant growth stage. Population density of soybean sprout rotting bacteria on soybean leaves was $0~10^3CFU/cm^2$. Population density of soybean sprouts rotting bacteria was related to cultivating area, but not related to plant growth stage. Cultivar and population density of soybean sprout rotting bacteria were less corelated, and varied by plant growth stages and plant parts. Erwina cypripedii, E. carotovora subsp. carotovora, Xanthomonas campestris pv. glycines, Staphylococcus sp., and Micrococcus sp. were identified as pathogenic bacteria causing soybean sprout rot. In generally population density of E. cypripedii, E. carotovora subsp. carotovora, Micrococcus sp., and X. campestris pv. glycines were high.

• Journal of Bio-Environment Control
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• v.9 no.2
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• pp.101-106
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• 2000

The practicality of utilizing ventilation pipe to enhance soybean sprout productivity by lowering commodity temperature and resultant sprout rot was tested. The ventilation pipes with holes for aeration were vertically installed inside of a sprout cultivation container prior to adding soaked soybean seeds. The time-series changes in sprout commodity temperature and resultant growth, yield, marketable sprout percentage, and rot of soybean sprouts were measured. The ventilation pipes effectively decreased sprout commodity decreased sprout commodity temperature by 4.7$^{\circ}C$. Ventilation pipes also enhanced sprout yield and marketable sprout percentage by 3.9% and 4.0%, respectively, while reducing sprout rot by 4.1%. In conclusion, ventilation pipe seems a practical and effective non-chemical method to enhance the productivity of soybean sprouts.

• Korean journal of food and cookery science
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• v.15 no.4
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• pp.382-387
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• 1999

This study was conducted to investigate the changes of mineral contents in radish leaves, soybean sprout and chwi namul by cooking method(boiling, steaming, sauting) and cooking time(1, 3, 5 min.). The residual rate of minerals(such as Calcium, Sodium, Potassium, Iron and Magnesium) in radish leaves, soybean sprout and chwi namul by 1 minute sauting was the range of 83.2 to 99.1%. It was shown that sauting was desirable method for all three vegetables. The residual rate by the cooking method was sauting, steaming, boiling, in that order, but boiling in radish leaves and chwi namul were more desirable method than steaming for Calcium use. At the cooking method and cooking time, Potassium content in soybean sprout was reduced remarkably in 5 minutes boiling. Boiling of radish leaves and soybean sprout showed that Sodium was reduced remarkably after 5 minutes of boiling, 54.4% for radish leaves and 19.9% for soybean sprout, respectively.

• Current Research on Agriculture and Life Sciences
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• v.27
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• pp.53-58
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• 2009

This experiment was conducted to determine the amount of asparagine content in soybean sprout soup as well as its effect on the regulation of blood alcohol concentration in human body. Asparagine content and alcohol concentration in blood were analyzed using the amino-acid analyzer and alcohol tester, respectively. Asparagine content in soybean sprout soup was highly significantly different among sprout soups sampled from different restaurants, although the boiling time and amount of sprout used were different among the samples. Significantly higher asparagine content was observed in soups with higher amounts of sprouts (2.81% in 500 grams of sprout) and 10 minutes after boiling with other solid ingredients in the soup. Lower concentration of alcohol in blood was recorded in persons with higher body weight. The asparagine+soybean extract (eaten immediately after drink) lowered the blood alcohol concentration significantly followed by only asparagine and control. The blood alcohol concentration after drinking become zero earlier (by 30 minutes), when asparagine+soybean extract or only asparagine was consumed as compared to control.

• Journal of Bio-Environment Control
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• v.6 no.4
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• pp.317-323
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• 1997

Incidence of soybean sprout rot by Erwinia carotovora subsp. carotovora was examined under several artificial conditions. Under higher temperatures over 3$0^{\circ}C$, disease incidence was increased and the rate of soft rot incidence was 22% at 35$^{\circ}C$. Artificial injuries of inner cotyledon and seed coat induced the disease above 70% and inhibited the soybean sprout growth. Relative humidity above 90% increased the soft rot to 33% and inhibited soybean sprout growth. When the leaked water collected from soybean sprout was reused for irrigation, the disease incidence was increased.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.3
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• pp.189-195
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• 2002

Soybean sprout trails were evaluated for soybeans for sprout (Glycine max) and wild soybeans (Glycine soja). Soybeans for sprout are characterized with small seed size of 10-14 grams per 100 seeds and are traditionally used only for producing sprouts. No difference was observed in hypocotyl length and sprout yield between two types of soybean. (However total length, hypocotyl hypocotyl, body weight, and the rate of good quality were greater in soybeans for sprout.) Number of lateral roots and root length were greater in wild soybeans. Contents of crude oil, oleic acid, and sucrose were higher in soybean for sprout, while that of protein and linoleic acid were higher in wild soybeans. There was no difference in sprout isoflavone content. Out of 17 amino acids analyzed, 10 amino acids such as aspartic acid, lysine, and arginine. etc. showed significantly Higher content in wild soybeans. Hypocotyl color of sprouts estimated by color meter based on the degree of L(brightness), a(red), and b(yellow) values were better in wild soybeans than soybean for sprout.

• The Korean Journal of Food And Nutrition
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• v.13 no.4
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• pp.312-318
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• 2000

This study was conducted to investigate the effects of soybean germ of Glycin max Merrill on the improvement of lipids in the serum of cholesterol supplemented diet induced dietary hyperlipidemic rats fed for 4 weeks. The experiment diet was mixed with 0.75% cholesterol recieved a basic diet mixed with 0.75% cholesterol. No significance differences in efficency of food, liver kidney and heart. Concentration of total cholesterol and LDL cholesterol in serum was significantly lower in the methanol extracts of soybean sprout than the cholesterol supplemented diet group. In the ratio of HDL-cholesterol concentration to total cholesterol concentration, the methanol extracts of soybean sprout administration group was higher percentage than the ocher groups. Atherosclerotic index was lower in the methanol extracts of soybean sprout group than in the cholesterol group. Concentration of phospholipid in serum was more decreased in the methanol extracts of soybean sprout group than in the cholesterol diet group. From these results, the methanol extracts of soybean sprout were effective on the improvement of the lipid compositions in the serum of high fat diet induced dietary hyperlipidemic rats.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.3
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• pp.303-307
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• 2008

Cotyledon Black Decay (CBD) on soybean sprout mimics the black spot due to microbial infection. CBD, not visible or predictable at seedlot state, for some reason, shows up exclusively on cotyledon of soybean sprout during sprouting process. Such an incidence rate fluctuated from 0.8 to 19.5% over three years from 2004. We suspected some pod-infecting anthracnose fungi and/or pod-blight pathogen, or pod-sucking bean bug, one of the major pests of soybean, might have involved, of which we ruled out fungal pathogen because it was preventable through heat treatment, a proven method for seedlot disinfestation. The healthy seeds artificially fed by bean bug for one to seven days were sprouted, and 6 to 41% of the soybean sprout revealed the CBD mimic to those occurred in soybean sprout from previous commercial seedlot screening experiments. This finding is the first report to confirm that bean bug damage to pod at $R_8$ stage is directly responsible for the CBD, which did not concur with any other deleterious effects on sprouting such as reduction in hypocotyls elongation and rooting except unsightly sprout quality. However, earlier feeding either at green pod or greenish yellow pod stage ($R_6$ -early $R_7$ stage) resulted in rather severe damages, which strikingly reduced hypocotyls growth to about one forth to about two third, as well as the reduction in rates of seed germination.