• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.134-134
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• 2022

Mungbean is used for not only seed but sprout, so, consumption of mungbean has been on the rise in Korea. Life cycle of mungbean tends to be short among Legume. For that reason, Mungbean can be harvested for various cropping system and season per regions and farmers regardless of sowing date and harvesting date. So, Prior research is needed about growth characters and life cycle of mungbean per sowing period. Mungebean cultivar 'Dahyun' and 'Sanpo' supplied by Korea Agriculture Technology Promotion Agency(KOAT) is cultivated in wagner pot. Sowing period is proper time of seeding in Jeollanam-do and Gyeongsangnam-do that is major cultivation region of mungbean in korea from early May to mid July every 2 weeks. Length at maturity stage tends to increase from early May(sowing date: 4^th May) to early July(sowing date: 5^th July), but after that, It tends to decrease from mid July(sowing date: 19^th July). Number of branches and nods shows a similar trend. Length of pod has no tendency and no difference per sowing date. Number of pod per plants has also no tendency per sowing date. Test plots sowing in late May has the most number of pods.(Sanpo 22.9pods, Dahyun 16.8) Number of seeds per pod tends to increase to late May and Test plots sowing in mid July has the most number of seed per pod. In case of sowing at early May, Days of emergence is 7d. its summation of temperature is 132.2℃. After that, it tends to decrease to mid June. After mid June, Days of emergence is fixed to 3d. Average temperature growing up in this season, Summation of temperature from sowing to emergence takes the lowest point in test plots sowing in mid June.(Sanpo 88.6℃, Dahyun 88.6) Days of flowering tends to fasten from early May to mid July. Two cultivar shows same level. Days of maturity tends to fasten to mid June, after that tends to slow. In case that many research results about growth characters and life cycle mungbean per sowing period are drawn, it is expected that it result in increase of cultivation area and income of farmer.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.81-81
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• 2020

본 연구는 새싹인삼(Panax ginseng sprout) 재배용으로 이용하기 위한 묘삼(종삼)의 장기 저온저장 시 사용되는 필름의 종류가 묘삼의 출아율이나 생육에 영향을 미치는 지 조사하기 위해 수행되었다. 포장에 사용된 필름은 다공성물질인 제올라이트와 페그마타이트가 5% 함유된 50㎛ low density polyethylene(기능성 LDPE) 필름과 nylon/polyethylene 80 ㎛(Ny/PE) 필름에 묘삼을 100g씩 포장한 후 골판지 상자에 담아 -2℃의 저장고에서 10개월 동안 저장하면서 8개월째부터 2개월마다 시료를 꺼내어 5℃에서 5일 동안 온도 순화를 시킨 후 1차 조사에서는 육안조사로 실험실에서 건전한 묘삼과 부패 묘삼을 조사하였고, 건전한 묘삼만 새싹인삼 수경재배 농가에 재식한 후 2차 조사로 묘삼의 출아율과 출아된 묘삼이 새싹인삼으로 건전하게 생육한 비율을 조사하였다. 조사결과 Ny/PE 필름에 8개월 저장된 묘삼의 경우 1차 육안검사 시 뇌두부위가 검게 변하여 묘삼이 대부분 고사된 것으로 조사되었다. 필름 개봉 전 필름 내부의 O₂ 및 CO₂ 가스농도를 측정한 결과 O₂ 농도는 1.91%, CO₂ 농도는 38.9%로 측정되었고, 필름 개봉 시 알코올 냄새의 이취가 나는 것으로 보아 Ny/PE 필름으로 포장 된 묘삼이 -2℃의 저온저장기간 동안에도 호흡을 하면서 필름 내 산소를 완전히 소모하고 혐기적인 환경으로 변화시켜 대사활동이 불가능하였기 때문에 뇌두의 싹이 고사된 것으로 조사되었다. 반면 기능성 필름으로 포장한 묘삼은 저장 8개월에 정식 후 74.3%의 출아율을 보였고, 건전한 새싹인삼의 비율은 67.2%로 조사되었다. 정식 전·후 묘삼의 개체수 대비 건전 출아율의 비율은 56.7%를 나타내어 Ny/PE 필름으로 포장한 묘삼의 결과와 큰 차이를 보였다. 기능성필름의 개봉 전 필름 내부의 O₂ 및 CO₂ 가스농도를 측정한 결과 O₂ 농도는 12.9%, CO₂ 농도는 4.6%로 측정되어 묘삼의 저온저장 시 필름을 적용할 경우 공기의 유동이 원활이 이루어져 내부 O₂ 농도를 일정 수준 이상 유지해주는 필름을 사용하는 것이 묘삼의 출아와 생육에 좋은 것으로 조사되었다.

• Journal of Bio-Environment Control
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• v.33 no.2
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• pp.99-106
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• 2024

The objective of the experiment was to determine the sprout emergence and asparagus seedling growth characteristics of six cultivars ('Apollo', 'Atlas', 'Grande', 'Purple Passion', 'UC157', and 'Walker Deluxe') according to growth stage to select suitable cultivar in the Korean climate condition. As a result of examining the growth characteristics of the seedlings of each cultivar during the dormancy, bud break, early fern growth and fern establishment stages, green asparagus cultivar 'UC157' had a higher number of spears, number of roots and buds, root length, and fresh and dry weights of root than the other cultivars. Green asparagus cultivars, 'Atlas' and 'UC157' were released from dormancy considerably earlier than other cultivars. In contrast, a purple asparagus cultivar 'Purple Passion' was showed to lower growth characteristics than those of other cultivars. Proper thermal insulation will be required when the seedling stage of asparagus is damaged by low temperature in winter. These results indicated that the 'UC157' cultivar showed characteristics of good seedlings and bud break compared to other cultivars and is thus expected to have the highest yield among all cultivars tested.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.3
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• pp.172-178
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• 2004

When soybean sprouts aye grown in the closed condition (where the ratio of $\textrm{O}_2$ and $\textrm{CO}_2$ is 7 ： 3), amount of $\textrm{CO}_2$ is increased and $\textrm{O}_2$ is decreased with the passage of time. At the same time, the amount of ethylene is automatically increased. By increasing the concentration of ethylene gas up to 0.5-1.0 ppm in the growth room, the length of sprouts was restricted to 6-7 cm and the thickness of sprouts was increased to 2.70$\pm$0.30 mm. The production of good quality sprouts which were fat and short was possible without application of any growth regulators such as indole-3-acetic acid known to have accumulation problem in humane body. To maintain the freshness during the transportation and prevent sprouts from rotting and bad smell at market, cold storage at 2-$5^{\circ}$ and airtightness which will restrict photosynthesis and respiration (higher than $10^{\circ}$) are needed. The freshness of sprouts is depended on the increase of $\textrm{CO}_2$ and the depletion of $\textrm{O}_2$ in the package. When the sprouts were stored below 1$0^{\circ}C$ (preferably below 8$^{\circ}C$), the concentration of $\textrm{CO}_2$ in the package remained below 30% for more than 60 hours, which was possible to keep sprouts in freshness without any offensive odor, But sprouts were maintained at $13^{\circ}$ for more than 25 hours, the concentration of $\textrm{CO}_2$ increased over 30% and produced an offensive odor. The little amount of $\textrm{O}_2$ gas was existing for 30 hours at $5^{\circ}$ but it was disappeared completely within 7 hours over $10^{\circ}$ and the sprouts became rot and produced severe offensive odor.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.12
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• pp.1958-1965
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• 2013

This study is conducted to investigate the contents of ${\beta}$-carotene and retinol in bap (e.g. gimbap, bokkeumbap, sushi, deopbap, bibimbap), guk (e.g. sundaeguk, seonjiguk), tang (e.g. galbitang, chueotang), and jjigae (e.g. dongtaejjigae, kimchi jjigae with port) by using saponification extraction and HPLC analysis. The samples were collected from six regions in Korea (Gangwon-do, Gyeonggi-do, Gyeongsang-do, Seoul, Jeonla-do, Chuncheong-do). In bap, the ${\beta}$-carotene contents of kimchi gimbap (234.459~719.180 ${\mu}g/100g$), bibimbap (200.091~489.867 ${\mu}g/100g$) and pork deopbap (228.876~778.591 ${\mu}g/100g$) were higher than that of sushi (0.000~41.234 ${\mu}g/100g$), and jajangbap (4.833~28.141 ${\mu}g/100g$). The retinol contents of bap was 0.000~60.418 ${\mu}g/100g$, among which, omelet rice (13.974~60.418 ${\mu}g/100g$) showed the highest amount of retinol. Among the analyzed guk (tang) and jjigae, higher contents of ${\beta}$-carotene were observed in chueoutang (346.261~843.947 ${\mu}g/100g$), kimchi jjigae with pork (178.558~352.604 ${\mu}g/100g$) and altang (169.443~175.287 ${\mu}g/100g$). The retinol of guk (tang) and jjigae were not detected to doganitang, gomtang, naejangtang, chueotang and soy sprout haejangguk.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.2
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• pp.201-207
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• 2014

The changes of growth characteristics and antioxidant activity for selection of optimum germinated temperature on common bean (Phaseolus vulgaris L.). Common beans (IT100888, IT102849, and IT231267) were cultivated at $20^{\circ}C$, $23^{\circ}C$ and $25^{\circ}C$ during 5 days of germination. The range of whole length, hypocotyls length, thickness, abnormal germination and yield rate of sprouts was 7.27~27.62 cm, 3.10~18.86 cm, 1.80~2.27 mm, 5.54~18.34% and 205.95~ 618.71%, respectively. Antioxidant activities of common beans with germination temperature investigated. Common beans (IT100888, IT102849, and IT231267) germinated at $20^{\circ}C$, $23^{\circ}C$ and $25^{\circ}C$ during 5 days, and then extracted with 80% ethanol, and analyzed for total polyphenol content, DPPH and ABTS radical scavenging activity. Total polyphenol content increased from 474 mg GA eq/100 g sample for IT231267 to 1364 mg GA eq/g sample for $23^{\circ}C$ of germination. DPPH radical scavenging activity of IT102849 increased from 189mg Trolox eq./100 g sample ($20^{\circ}C$) to 1073mg Trolox eq./100 g sample ($23^{\circ}C$) also ABTS radical scavenging activity of IT234267 increased from 479 mg Trolox eq./100 g sample ($20^{\circ}C$) to 1134 mg AA eq/100 g ($23^{\circ}C$). These results suggest that germination temperature for increasing antioxidant activities may be $23^{\circ}C$.

• Korean Journal of Food Science and Technology
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• v.43 no.1
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• pp.91-97
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• 2011

This study was performed to assess the microbiological quality and potential health risk of fresh-cut produce and organic vegetables sampled from supermarkets and department stores in Korea. A total of 96 samples comprised three types of fresh-cut produce (sprouts, mixed-vegetables, fruit) and three types of organic vegetables (lettuce, perilla leaf, green pepper). The samples were analyzed for total viable cell counts, coliforms, Enterobacteriaceae, Escherichia coli, Salmonella spp., Listeria monocytogenes, Vibrio parahaemolyticus, Bacillus cereus, and Staphylococcus aureus. The microbiological counts of fruit were very low. Sprouts were highly contaminated by total viable cell counts ($8.3{\pm}0.57$ log CFU/g), Enterobacteriaceae ($7.1{\pm}0.76$ log CFU/g), and coliforms ($4.9{\pm}0.40$ log MPN/g), and showed a high incidence level of B. cereus ($2.9{\pm}0.48$ log CFU/g). Of the fresh-cut produce analyzed, six (13.6%) mixed-vegetable salads were E. coli positive. S. aureus was detected in only one sprout sample and one mixed-vegetable salad, and its contamination levels were under 2 log CFU/g, which is appropriate for Korean standards (<3 log CFU/g) of fresh-cut produce. Of the organic vegetables, lettuces were highly contaminated by total viable cell counts ($6.4{\pm}0.74$ log CFU/g), Enterobacteriaceae ($5.7{\pm}0.98$ log CFU/g), and coliforms ($3.7{\pm}1.72$ log MPN/g). Two (13.6%) organic lettuce and one (7.1%) perillar leaf sample were E. coli positive, and S. aureus was detected in one lettuce and two perilla leaf samples. Salmonella spp., Vibrio parahaemolyticus, and Listeria monocytogenes were not detected in any of the fresh-cut produce or organic vegetables analyzed.

• Korean Journal of Food Science and Technology
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• v.42 no.3
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• pp.355-360
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• 2010

Vibrio parahaemolyticus (V. parahaemolyticus), which is commonly found in raw seafood, causes gastroenteritis in humans. Rapid and effective methods have been developed as culture methods require up to 5-7 days. In this study, real-time PCR was compared with the standard culture method for detecting V. parahaemolyticus in seafood and radish sprout samples. Five hundred grams of the samples were artificially contaminated with V. parahaemolyticus then divided into 20 samples. The samples were incubated in alkaline peptone water and then streaked onto thiosulfate-citrate-bile saltssucrose agar. Biochemical tests for suspicious colonies were performed using the API 20NE strip. In parallel, real-time PCR was performed targeting the toxR gene using the enrichment broth. The real-time PCR was sensitive in discriminating V. parahaemolyticus from other foodborne pathogens. The detection limit of the real-time PCR was $10^3\;CFU/mL$ in phosphate-buffered saline. Although the real-time PCR detected more positive samples (76 out of 180, 42%) than the culture method (66 out of 180, 37%), there was no significant statistical difference (p>0.05) between the two methods. In conclusion, real-time PCR assays could be an alternative to the standard culture method for detecting V. parahaemolyticus in seafood and radish sprouts, which has many advantages in terms of detection time, labor, and sensitivity.

• Korean Journal of Food Science and Technology
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• v.42 no.3
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• pp.329-334
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• 2010

Escherichia coli O157:H7 causes hemolytic uremic syndrome and hemorrhagic colitis in humans. The objectives of this study were to produce monoclonal antibody(MAb) against E. coli O157:H7 and to develop an enzyme linked immunosorbent assay(ELISA) for the rapid detection of E. coli O157:H7 in agri-stockbreeding. The characterization of MAb produced from hybridoma cell (HKEC 4G8-5) was validated by ELISA and Western blot. The produced MAb was specific to E. coli O157:H7 and showed weak cross-reaction to Staphylococcus aureus. The detection limit of ELISA based on 4G8-5 MAb was $10^5\;cell/mL$. Although the ELISA could not detect E. coli O157:H7 in the meat and sprout samples inoculated with $1{\times}10^1\;cell$/10 g without enrichment, the same samples after enrichment for 6 hr were confirmed to be positive by ELISA. These results indicated that the ELISA combined with short enrichment (6 hr) is useful tool for rapid screening of E. coli O157:H7 in various samples.

• Korean journal of food and cookery science
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• v.15 no.1
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• pp.16-22
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• 1999

The relation between food intakes and dental caries was investigated with 211 primary school students (83 boys and 128 girls) in Kumsan area. The results were as follows. The rate of dental caries of the subjects was 65.4%. 83.9% of the subjects had a snack, 69.5% of students having a snack and 44.1% of students having not snack was dental caries (p<0.001). For tooth brushing numbers per day, 38.4% of subjects was one time, and 61.6% was more than two times. For tooth brushing time, 31.8% had before meals and 68.2% after meals. The intake frequency rate of main food was cooked rice 33.30%, ramyun 14.81%, bread 9.57%, rice cake 6.16%, nodule 4.88% in good group, and cooked rice 27.04%, rice cake 12.04%, ramyun 8.50%, bread 8.11%, fried rice 5.49% in dental caries group. The intake frequency rate of a side dish was kimchi 13.88%, egg 6.21%, dried laver 5.51%, fish paste 5.51%, fish 5.03%, kongjaban 0.44% in good group, and potato soup 6.86%, cucumber 6.56%, sprout soup 5.78%, bean curd 5.33%, sesame leaf 5.21%, lettuce 1.23% in dental caries group. The intake frequency rate for snack was ice cream 9.85%, biscuit 7.35%, gum 6.17%, chestnut 6.16%, apple 5.81%, milk 5.56%, yogurt 4.86%, gypo 4.39%, candy 4.15%, chocolete 3.91% in good group, and biscuit 10.00%, ice cream 6.75%, candy 5.88%, fruit canned food 5.70%, milk 5.41%, corn 5.00%, banana 5.00%, peanut 4.42%, fritter 4.39% in dental caries group. The rate of cariogenic food, detergent food, protective food in a side dish were 4.0%, 40.8%, 55.1%o in good group, and 11.5%, 43.9%, 44.6% in dental group respectively (p<0.001). The rate of cariogenic food, detergent food, protective food in a snack 67.8%, 10.4%, 21.7% in good caries group, and 75.3%, 8.8%, 15.9% in dental caries group respectively (p＜0.001).