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Production of Monoclonal Antibody against Escherichia coli O157:H7 and Development of Enzyme Linked Immunosorbent Assay  

Ryu, Hee-Jeong (Division of Applied Life Science (BK21 program), Gyeongsang National University)
Kim, Jeong-Sook (Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Kyeong-Yeol (Division of Applied Life Science (BK21 program), Gyeongsang National University)
Nam, Bo-Ram (Division of Applied Life Science (BK21 program), Gyeongsang National University)
Nam, Min-Ji (Division of Applied Life Science (BK21 program), Gyeongsang National University)
Shim, Won-Bo (Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Nam-Soo (Food Bio-nano Technology Research Group, Korea Food Research Institute)
Cho, Yong-Jin (Food Bio-nano Technology Research Group, Korea Food Research Institute)
Chung, Duck-Hwa (Division of Applied Life Science (BK21 program), Gyeongsang National University)
Publication Information
Korean Journal of Food Science and Technology / v.42, no.3, 2010 , pp. 329-334 More about this Journal
Abstract
Escherichia coli O157:H7 causes hemolytic uremic syndrome and hemorrhagic colitis in humans. The objectives of this study were to produce monoclonal antibody(MAb) against E. coli O157:H7 and to develop an enzyme linked immunosorbent assay(ELISA) for the rapid detection of E. coli O157:H7 in agri-stockbreeding. The characterization of MAb produced from hybridoma cell (HKEC 4G8-5) was validated by ELISA and Western blot. The produced MAb was specific to E. coli O157:H7 and showed weak cross-reaction to Staphylococcus aureus. The detection limit of ELISA based on 4G8-5 MAb was $10^5\;cell/mL$. Although the ELISA could not detect E. coli O157:H7 in the meat and sprout samples inoculated with $1{\times}10^1\;cell$/10 g without enrichment, the same samples after enrichment for 6 hr were confirmed to be positive by ELISA. These results indicated that the ELISA combined with short enrichment (6 hr) is useful tool for rapid screening of E. coli O157:H7 in various samples.
Keywords
E. coli O157:H7; monoclonal antibody; ELISA;
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Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 1
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