• 한국항공우주학회지
• /
• 제34권6호
• /
• pp.51-58
• /
• 2006

횡단 유동장내에서 일어나는 분무 현상에 대하여 분사각 변화에 대한 특성과 단일 노즐 형상에서 노즐의 크기와 길이에 대한 분무 특성을 연구하였다. 노즐은 단일 구멍으로 직경이 0.5 ㎜이고 노즐 대 노즐길이의 비(L/D)는 1.0에서 6.0이며 이미지는 고해상 줌 렌즈를 이용한 CCD 카메라를 통해 얻었으며, SMD와 액적의 속도는 PDPA와 상용 프로그램인 Image Express를 사용하였다. 액체 제트의 궤적은 웨버수와 모멘텀비, 노즐형상 변화(L/D)에 영향을 받아 액주가 후방으로 휘어지는 현상이 나타났다. 분사각이 낮을 때(${\theta}$ < $90^{\circ}$)에 노즐 형상보다는 웨버수 증가가 액체 제트의 궤적에 더 크게 영향을 미쳤으며, 분사각이 높을 때(${\theta}$ > $90^{\circ}$)에 노즐 형상 변화에 의한 분열점 변화가 액체 제트의 궤적에 더 크게 영향을 미쳤다.

• Mass Spectrometry Letters
• /
• 제5권1호
• /
• pp.24-29
• /
• 2014

An advanced and reliable high performance liquid chromatography (HPLC)/ultraviolet detector (UV)/ion-trap time-of-flight (IT-TOF) mass spectrometry was developed for the simultaneous quantification of 19 marker compounds in Bang-poong-tong-sung-san (BPTS), a traditional oriental prescription. Various parameters affecting HPLC separation and IT-TOF detection were investigated, and optimized conditions were identified. The separation was achieved on a Capcell PAK C18 column ($1.5mm{\times}250mm$, $5{\mu}m$ particle size) using a gradient elution of acetonitrile and water containing 0.1% formic acid at a flow rate of 0.1 mL/min. The column temperature was maintained at $40^{\circ}C$ and the injection volume was $2{\mu}L$. IT-TOF system was equipped with an electrospray ion source (ESI) operating in positive or negative ion mode. The optimized electrospray ionization parameters were as follows: ion spray voltage, +4.5 kV (positive ion mode), or -3.5 kV (negative ion mode); drying gas ($N_2$), 1.5 L/min; heat block temperature, $200^{\circ}C$. Automatic $MS^n$ (n = 1~3) analyses were carried out to obtain structural information of analytes. Elemental compositions and their mass errors were calculated based on their accurate masses obtained from a formula predictor software. The marker compounds in BPTS were identified by comparisons between $MS^n$ spectra from standards and those from extracts. Moreover, the libraries of $MS^2$ and $MS^3$ spectra and accurate masses of parent and fragment ions for marker compounds were constructed. The developed method was successfully applied to the BPTS extracts and identified 17 out of 19 marker compounds in the BPTS extracts.

• Food Science and Biotechnology
• /
• 제18권6호
• /
• pp.1470-1475
• /
• 2009

The seed coat of the black soybean contains 3 main anthocyanins such as delphinidin-3-O-$\beta$-glucoside, cyanidin-3-O-$\beta$-glucoside, and petunidin-3-O-$\beta$-glucoside. As a part of our effort on discovering and breeding new black soybean cultivars which possesses specific anthocyanin component rich, we determined the anthocyanin profiles of the 2 cultivars recently developed soybean cv. Gaechuck #1 and cv. Gyeongsang #1, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and compared their content and identity with those of previously known 10 cultivar controls. The Cosmosil-$5C_{18}$-AR-II column were selected for the analysis because of the best peak separation. The column temperature was set up at $35^{\circ}C$. The mobile phase consisting of water containing 0.5%(v/v) formic acid and methanol gave good separation between the 3 anthocyanin analytes and internal standard (quercetin 3-O-$\beta$-rutinoside) and peaks with suppressed tail. The MS/MS spectra of each individual anthocyanin standard were detected in positive electron spray ionization (ESI) modes. It was disclosed that the anthocyanin contents of the soybean cv. Gaechuck#1 and cv. Gyeongsang#1 are roughly higher than those of the 10 controls.

• Asian-Australasian Journal of Animal Sciences
• /
• 제20권11호
• /
• pp.1770-1776
• /
• 2007

Beef carcasses were examined to explore the effects of spinal cord removal and washing on central nervous system tissue (CNST) decontamination of the surface during the slaughtering process. A total of 15 carcasses were split by sawing centrally down the vertebral column and left sides of split carcasses were used for analysis. Samples were collected by swabbing the surface from 4 defined parts on the interior and 4 on the exterior of carcasses from the abattoir and analyzed using an ELISA-based test. The results showed that automatic and manual spray washing decreased CNST contamination, especially on the interior ventral parts of carcass surfaces (p<0.01), but did not decrease CNST on the interior dorsal parts. Increasing washing time to 60 s did not affect the reduction of CNST contamination. Samples following spinal cord removal prior to splitting showed lower calculated levels of "risk material" than the stated limit of detection (0.1%) of the ELISA kit on interior and exterior carcass parts (p<0.01). Therefore, spinal cord removal prior to splitting could be a very effective way to minimize CNST contamination of beef carcasses.

• Ocean and Polar Research
• /
• 제35권2호
• /
• pp.107-121
• /
• 2013

The underwater glider is an autonomous vehicle that can glide through the ocean interior by using a pair of wings attached to its body and can move up and down through the water column by changing its buoyancy. As of now, there are three widely-used gliders, namely, the Spray that was co-developed by Scripps Oceanographic Institution and Woods Hole Oceanographic Institution, the Slocum produced by the Webb Research Cooperation, and the Seaglider that was produced by the University of Washington. In this paper, I will introduce these three gliders and discuss the principles and procedures related to glider operation as well as the application and extendability of modern physical and bio-geochemical sensors to gliders. My experiences in developing a glider for measuring ocean turbulence and testing it 7 times during 12 days are shared in this paper. On the basis of my experiences and knowledge, different kinds of aspects that should be considered for successful glider operation are discussed. In addition, a suggestion is made as to what would be the ideal way to operate underwater gliders in the East/Japan Sea. At the end, the current status of active glider operation teams is presented and the efforts to proceed toward future gliders are briefly introduced.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2011년도 제41회 하계 정기 학술대회 초록집
• /
• pp.123-123
• /
• 2011

알루미늄은 경량 금속으로 부식 저항력이 높아 철을 부식으로부터 보호하기 위한 표면처리 소재로 사용되고 있다. 철의 부식을 방지하기 위해서 알루미늄을 코팅하는 경우, 코팅 방법은 용융도금법이 주로 사용되고 있으며, 알루미늄을 빛의 반사막으로 활용하는 경우 진공 중에서 물리기상증착(physical vapor deposition; PVD)법을 사용하기도 한다. 알루미늄 박막을 물리기상 증착으로 코팅하면 박막성장 초기에 핵(nucleus)을 형성하고, 형성된 핵을 중심으로 주상정(column)으로 박막이 성장하는 것이 일반적이다. 알루미늄 박막의 주상정과 주상정 사이에 공극(pore)이 존재하기 때문에 알루미늄 박막을 부식방지 막으로 이용하기 위해서는 두께를 증가시켜야 한다. 본 연구에서는 스퍼터링(unbalanced magnetron sputtering)을 이용하여 치밀한 조직을 갖는 알루미늄 박막을 코팅할 수 있는 공정변수를 도출하고, 치밀한 알루미늄 조직이 철의 부식에 미치는 영향을 평가하였다. 기판은 냉연강판(cold rolled steel sheet)이 사용되었으며, 알루미늄 타겟의 크기는 직경 4 inch이었다. 알루미늄 박막의 미세조직과 밀도에 영향을 주는 공정변수를 확인하기 위해서 스퍼터링 파워, 공정 압력, 외부 자기장 세기 등의 조건을 변화시켜 코팅을 실시하였다. 알루미늄 박막의 밀도 변화에 가장 큰 영향을 준 공정변수는 외부 자기장의 세기와 방향이었다. 알루미늄 박막이 약 3 ${\mu}m$의 두께로 코팅된 냉연강판을 염수분무시험(salt spray test, 5% NaCl)으로 부식특성을 평가한 결과, 시험을 시작한 후 120시간 후에도 적청이 발생하지 않았다. 이러한 결과는 기존의 동일한 두께를 갖는 알루미늄이 코팅된 강판의 내부식 특성의 2배의 성능을 보여준다.

• 한국수산과학회지
• /
• 제42권1호
• /
• pp.15-19
• /
• 2009

A simple and sensitive method for erythromycin quantification by liquid chromatography electrospray mass spectrometry (LC-MS/MS) in fishery products was developed. Samples were extracted by liquid-liquid extraction using 70% acetonitrile. Lipids were removed by acetonitrile saturated hexane. LC separation was performed on a Shiseido UG C-18 column ($150\;mm{\times}2.0\;mm$ internal diameter.) with a gradient system of 0.2% acetic acid-acetonitrile containing 0.2% acetic acid as a mobile phase at flow rate of 0.2 mL/min. The mass spectrometer was operated in selected reaction monitoring with positive electro-spray interface. Transitions were monitored a m/z $734{\to}577$ and $734{\to}158$, with m/z $734{\to}577$ chosen for quantification. Recovery of erythromycin from fish and shrimp fortified at the 10 ng/mL, 50 ng/mL and 100 ng/mL were 91.6-109.4%, 84.4-111.2% and 98.8-109.6% with high precision, respectively. Limits of quantification and limits of detection of erythromycin in both fish and shrimp were 10.0 ng/mL and 1.0 ng/mL, respectively. This analysis method for erythromycin has been proposed for registration in the Korean Official Methods of Food Analysis and has been utilized for fishery products analysis by the Korea Food and Drug Adminstration and the National Fisheries Products Quality Inspection Service.

• 한국응용곤충학회지
• /
• 제14권1호
• /
• pp.29-33
• /
• 1975

저항성유기인계농약인 fenitrothion 유제를 8개품종이 수도체에 살포시용하고, 부착 및 침투된 주성분의 경시적변화와 곡위중의 집적잔류량을 GLC로 분석, 검토한 바 그 결과를 요약하면 다음과 같다. 1) FTD/GLC에 있어서 $10\%$ Silicone DC-200 column으로 nano-gram 이하까지 fenitrothion 주성분의 검출이 가능한 조작조건을 밝혔다. 2) 출수전의 수도체에 $0.1\%$ fenitrothion을 반당 20l의 농산로 살포한 바, 전엽에 평균 15.32ppm이 부착, 24시간후에 1.67ppm(약 $11\%$)이 잔존하였고, 살포 24시간후의 침투량은 6.75ppm($44\%$)으로, 24 시간경과후 2.61ppm($39\%$이 잔존하고 그후의 감소는 완만하며, 침투분의 잔존율이 높았다. 3) 엽신과 엽초별 침투량은 엽신이 월등히 높았으나 잔존율은 비등하였고, 침투 3일이내에 급격한 감소(약$90\%$)를 보이고, 그 후는 완만한 감소로 10일후에는 약 $3\%$의 잔존율을 유지하였다. 4) 곡위중의 fenitrothion 잔류량은 왕겨에 0.02-0.05ppm(평균 0.04ppm), 현미에 0.01 ppm-0.02ppm(평균 trace)수준으로 왕겨중함량의 약 $22\%$ 해당하며, WHO/FAO의 권고잔류허용량 (과채류는 0.1-0.5 ppm, 곡류는 미설정)에 훨씬 미달되는 수치였다.

• Journal of Ginseng Research
• /
• 제42권2호
• /
• pp.175-182
• /
• 2018

Background: The impact of fungicide azoxystrobin, applied as foliar spray, on the physiological and biochemical indices and ginsenoside contents of ginseng was studied in ginseng (Panax ginseng Mey. cv. "Ermaya") under natural environmental conditions. Different concentrations of 25% azoxystrobin SC (150 g a.i./ha and 225 g a.i./ha) on ginseng plants were sprayed three times, and the changes in physiological and biochemical indices and ginsenoside contents of ginseng leaves were tested. Methods: Physiological and biochemical indices were measured using a spectrophotometer (Shimadzu UV-2450). Every index was determined three times per replication. Extracts of ginsenosides were analyzed by HPLC (Shimadzu LC20-AB) utilizing a GL-Wondasil $C_{18}$ column. Results: Chlorophyll and soluble protein contents were significantly (p = 0.05) increased compared with the control by the application of azoxystrobin. Additionally, activities of superoxide dismutase, catalase, ascorbate peroxidase, peroxidase, and ginsenoside contents in azoxystrobin-treated plants were improved, and malondialdehyde content and $O_2^-$ contents were reduced effectively. Azoxystrobin treatments to ginseng plants at all growth stages suggested that the azoxystrobin-induced delay of senescence was due to an enhanced antioxidant enzyme activity protecting the plants from harmful active oxygen species. When the dose of azoxystrobin was 225 g a.i./ha, the effect was more significant. Conclusion: This work suggested that azoxystrobin played a role in delaying senescence by changing physiological and biochemical indices and improving ginsenoside contents in ginseng leaves.

• 한국화재소방학회논문지
• /
• 제30권3호
• /
• pp.7-15
• /
• 2016

단면형상계수는 세계적으로 흔하게 활용되는 공학적 내화구조 성능평가 방법이지만 국내의 경우 단면형상계수의 적용을 위한 제도가 아직 마련되어 있지 못하다. 실제 우리나라의 대부분의 철골조 건축물에서 원형 강관의 사용을 흔히 볼 수 있으나, 인정기관으로부터 성능을 인정받은 뿜칠 피복 철골 보 및 기둥은 대부분이 H형강으로 한정되어 있다. 비재하 시험의 특성상 H형강은 관련규정에 따른 시험규격으로 부재에 대한 평가를 수행하여 크기의 제한 없이 인정범위가 부여되나, 이형 강관의 경우는 형상별로 별도의 내화성능에 대한 인정을 받아야 한다. 본 연구에서는 합리적인 내화구조의 인정범위를 설정할 수 있도록 단면형상계수를 활용하여 뿜칠 피복재의 원형 강관의 적용을 위한 표준 시험체 규격을 제시하고자 한다.