• 제목/요약/키워드: Sprague Dawley rats

검색결과 3,877건 처리시간 0.023초

Sprague-dawley(SD) 랫드에서 natural killer cell의 분리 ·동정 및 형태적 특징 (Isolation and morphological characterization of natural killer cell in the sprague-dawley(SD) rats)

  • 강경선;이영순
    • 대한수의학회지
    • /
    • 제32권2호
    • /
    • pp.245-250
    • /
    • 1992
  • This study was performed to demonstrate the presence of large granular lymphocyte(LGL) in Sprague-Dawley(SD) rats and morphologically observe NK cell and also establish the method of isolation of natural killer cell in SD rats. By percoll discontinuous density gradients centrifugation, highly enriched LGL population were shown to fraction 2(border line between 44.2% and 50.8%). LGL were shown to bind selectively to YAC1 mouse lymphoma cell. This fraction expressed very high NK cell cytolysis. Therefore, we thought that LGL have NK activity in SD rats. The Morphology of rat LGL is very similar to that of human LGL. These cells have an eccentric kidney-shaped nucleus. Their most distinctive feature was their cytoplasmic azurophilic granules. Another distinguishing feature of rat LGL was their high cytoplasmic : nuclear ratio. It was concluded that LGL played a role part in mediating natural killer activity in this species.

  • PDF

랫드에 대한 G. bimaculatus의 급성경구독성시험 (Acute Oral Toxicity of G. bimaculatus in Rats)

  • 김인선;안미영;류강선;이병무
    • Toxicological Research
    • /
    • 제18권4호
    • /
    • pp.397-400
    • /
    • 2002
  • This study was carried out to investigate the acute toxicity of G. bimaculatus in Sprague-Dawley rats. G. bimaculatus was administered orally at doses of 8, 40, 200, 1000 and 5000 mg/kg. in this study, number of deaths, clinical sign, body weights, and pathological examination were investigated for 14 days after administration of G. bimaculatus. The results indicate that G. bimaculatus did not show any toxic effect in rats and oral $LD_{50}$ value was over 5000 mg/kg in Sprague-Dawley rats.

Sprague-Dawley 랫트에 자연발생한 고환위축의 병리조직학적 관찰 (Histopathological observation of spontaneous testicular atrophy in Sprague-Dawley rat)

  • 손화영;김용범;하창수;강부현
    • 한국수의병리학회지
    • /
    • 제2권1호
    • /
    • pp.47-52
    • /
    • 1998
  • The incidence of spontaneous testicular atrophy and its morphological changes during stage-specific spermatogenesis were investigated in male Sprague-Dawley rats at 10, 19, and 32 weeks of age. The incidence of testicular atrophy was 0.2%(2/90) 7.9%(9/114) and 10%(4/40) in 4, 13 and 26 weeks respectively. The epididymis with testicular atrophy had low sperm density. In the minimally affected tests scattered tubules showed complete depletion of germ cells without stage specificity. Testes with moderate to severe testicular atrophy showed seminiferous tubules lined with dense Sertoli cell population. While Leydig cells in the interstitium appeared hypertrophy they were immunohistochemically negative for proliferating cell nuclear antigen a marker of cell proliferation.

  • PDF

참치뼈로부터 제조한 Hydroxyapatite 소결체의 랫드에 대한 급성피하독성시험 (Study on Acute Subcutaneous Toxicity of Hydroxyapatite Sinter Produced from Tuna Bone in Sprague-Dawley Rats)

  • 김세권;박표잠;김용태
    • 생명과학회지
    • /
    • 제11권2호
    • /
    • pp.97-102
    • /
    • 2001
  • This study was performed to evaluate the actue sbucutaneous toxicity of hydroxyapatite sinter produced from tuna bone in Sprague-Dawley(SD) rats. Hydroxyapatite sinter was administrated at dose levels of 5000, 2500, 1250, 625, 312.5 and 0 mg/kg. After single subcutaneous adiminstration to both sexes to both sexes SD rats, we observed rats for 14 days. Hydroxyapatite sinter did not induce any toxic signs inmortalities, clinical findings, body weights and gross findings of the rats. In view of result, it was impossible to estimate LD/ sub 50/ values in SD rats. In conclusion, these results suggest that hydroxyapatite sinter produced from tuna bone has no effect on acute subcutaneous toxicity in SD rats.

  • PDF

Sprague-Dawley 랫드를 이용한 미선나무주정추출물의 2주 반복 경구투여 독성평가 (Toxicity Assessment of Abeliophyllum distichum Nakai Ethanol Extract Orally Administered to Sprague-Dawley Rats for Two Consecutive Weeks)

  • 권순복
    • 한국식생활문화학회지
    • /
    • 제34권6호
    • /
    • pp.801-809
    • /
    • 2019
  • Abeliophyllum distichum Nakai is a deciduous shrub of a flowering plant in Oleaceae. It is an important plant resource and consists of only one species in the entire world. A. distichum Nakai is well known an edible, medicinal herb in its habitat districts, but the toxicological evaluation for the safe use of its extract is still insufficient. The study characterized the toxicity of an Abeliophyllum distichum Nakai ethanol extract in Sprague-Dawley (SD) rats and determined the safe dosage levels in a 13 weeks toxicity study. Abeliophyllum distichum Nakai ethanol extract was orally administered once daily for 2 weeks at 0, 500, 1,000 and 2,000 mg/kg/day to male and female SD rats. while recording the clinical signs of toxicity, body weight, food intake/consumption, eye test and urine analysis. Only the total protein frequency in the urine of male SD rats (p<0.05), the right ovary of the 500 mg/kg group (p<0.01) and the right adrenal gland of the 1,000 mg/kg group (p<0.05) in the female rats showed statistically significant changes. But no toxic effects were noted from repeated-dose administration of the Abeliophyllum distichum Nakai ethanol extract in the SD rats during the observation period. The post-mortem examinations showed no test substance-mediated changes. The hematological analysis and clinical blood chemistry data demonstrated no toxic effects from repeated-dose administration of Abeliophyllum distichum Nakai ethanol extract in the SD rats during the observation period. Based on these results, this data suggests that a dose of 1,000 mg/kg/day is a highest treatment to administer when conducting a further 13 weeks toxicity study.

랫드에서 인체 재조합 적혈구 조혈인자, rHu-EPO의 아급성정맥독성시험 (Subacute Intravenous Toxicity Study of Recombinant Human Erythropoietin(rHu-EPO) in Rats)

  • 김형식;곽승준;천선아;임소영;박현선;홍채영;김원배;김병문;안병옥
    • Toxicological Research
    • /
    • 제12권2호
    • /
    • pp.309-318
    • /
    • 1996
  • This study was conducted to investigate the subacute intravenous toxicity of rHu-EPO in Sprague-Dawley rats. rHu-EPO was daily administered to male and female rats for 30 days with different dose levels(100, 500, 2,500 unit/ kg). After the administration period of 30 days, 5 males and 5females rats per each dose group were assigned for recovery period of additional 30 days. There were no clinical signs compared with control group but slight decrease in spontaneous motor activities and locomotions were observed at high dose groups of males and females. In males, feed consumption was reduced at 500 unit/kg and body weight gain was retarded. In the administration sites of tail, focal congestion was observed in a few animals treated with rHu-EPO. No deaths were occurred during the course of study. In hematological examination. a significant increase of hemoglobin and hematocrit was observed in the males and females rats receiving high dose of rHu-EPO. rHu-EPO administration for 30 days showed a marked decrease in glucose concentration. At the highest dose groups, there was a significant incerase in the weights of spleens in both sexes. but this was considered to be related to its pharmacological activity. These results indicate that rHu-EPO does not induce any significant toxic effect on Sprague-Dawley rats for 30 days.

  • PDF

흰쥐에 대한 생모수의 아급성 경피독성시험 (Subacute Dermal Toxicity Study of Sangmosu in Rats)

  • 박현선
    • 약학회지
    • /
    • 제43권3호
    • /
    • pp.358-368
    • /
    • 1999
  • Subacute toxicity study was performed in Sprague-Dawley rats after daily dermal administration of Sangmosu (0.2, 1.0 and 5.0 g/kg) for one month. There were no clinical signs and pathological changes compared with control group. Bodyweights were not significantly changed between control and Sangmosu treatment groups. In histopathological examinations, there were some pneumonia in lung tissues at all groups of Sangmosu treatment including control, but it was not considered to be caused by Sangmosu. These results suggest that Sangmosu does not induce any significant subacute dermal toxicities in Sprague-Dawley rats.

  • PDF

Sprague-Dawley 랫드를 이용한 수태능 및 초기배 발생시험의 기초자료연구 (Background Data for Fertility and Early Embryonic Development Study in Sprague-Dawley Rats)

  • 김종춘;이상준;서정은;차신우;김충용;한정희;정문구
    • Toxicological Research
    • /
    • 제18권2호
    • /
    • pp.167-174
    • /
    • 2002
  • Historical control data have been shown to be valuable in the proper interpretation and validation of reproductive toxicology studies. The present data were compiled from rat fertility and early embryonic development studies conducted at Korea Institute of Toxicology during the 1994∼2001 period. These data were assembled in order to provide background information for the general and reproductive data collected in 11 fertility and early embryonic development studies using Sprague-Dawley rats obtain-ing from the Breeding Facility, Korea Institute of Toxicology, Korea. A total of 274 males and 274 females were used in these studies during the eight-year period. Parameters of fertility and early embryonic development included clinical sign, body weights, food consumption, organ weights, estrus cycle, copulation index, precoital time, fertility index, pregnancy index, sperm parameters, and early embryonic development parameters. Most of the values were comparable to the previous historical control data reported by other investigators. These data can be wed not only as a historical data base for the meaningful interpretation of data from reproductive and developmental toxicity studies, but also as a contribution to biological characterization of Sprague-Dawley rats.

Ktc:Sprague-Dawley 랫드의 안구에 자연발생한 amelanotic melanoma (Spontaneous intaocular amelanotic melanomas in ktc:Sprague-Dawley rats)

  • 강부현;손화영;하창수;김대용
    • 대한수의학회지
    • /
    • 제35권2호
    • /
    • pp.375-381
    • /
    • 1995
  • Two intraocular tumors were identified in low and medium dosed groups of a carcinogenicity study using 200 males and 200 females Sprague-Dawley rats in Screening & Toxicology Research Center, Korea Research Institute of Chemical Technology. The tumors were grossly observed as white or yellow, unilateral nodules. They were approximately $1-2{\times}3-5mm$ in size. The tumors located in the region of iris and/or ciliary body invaded peripheral cornea. The microscopic features were usually composed of spindle cells arranged in parallel, forming gently curving bundles or whorls. The spindle cells had poorly defined cell boundaries, scant to moderate cytoplasm, fusiform nuclei and indistinct nucleoli. Mitotic figures were rare and areas of necrosis were present. The spindle cells had positive immunoreactivity for S-100 protein and vimentin but negative for desmin, collagen and HMB-45 antibody. In special histochemical studies, the spindle cells react with Gomori's stain for argyrophih fibers, Prussian blue stain for iron but negative with Masson-Fontana's stain for melanin granules. Ultrastructurally, cytoplasmic premelanosomes were not observed in the tumor cells due to the poor preservation of tumor masses. Based on the results, the tumors were diagnosed as amelanotic melanoma.

  • PDF

Ktc: Sprague-Dawley 랫드의 장기중량치의 자료분석 (Reference Values of Organ Weights in Sprague-Dawley Rats)

  • 강부현;김일환;김용범;김영희;이현숙;하창수
    • 한국수의병리학회지
    • /
    • 제5권2호
    • /
    • pp.39-42
    • /
    • 2001
  • A reference range data base containing organ weight values on animals used in 4 week and 13 week toxicity tests is described. Data listed include the values of minimum, maximum, mean and standard deviation for each of the following organ weights. Organs: Brain, heart, liver, spleen, kidney, adrenal gland, testis, ovary. This study was undertaken to determine organ weight reference values of Ktc: SD rats

  • PDF