• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.1125-1129
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• 2005

Cloning of a 6.6-kb BamHI digested chromosomal DNA from S. griseus IFO13350 revealed the presence of an additional gene encoding a novel trypsin-like enzyme, named SprU. The SprU protein shows a high homology ($79\%$ identity, $88\%$ similarity) with the SGT protease, which has been reported as a bacterial trypsin in the same strain. The amino acid sequence deduced from the nucleotide sequence of the sprU gene suggests that SprU is produced as a precursor consisting of an amino-terminal presequence (29 amino acid residues), prosequence (4 residues), and mature trypsin consisting of 222 amino acids with a molecular weight of 22.94 kDa and a calculated pI of 4.13. The serine, histidine, and aspartic acid residues composing the catalytic triad of typical serine proteases are also well conserved. When the trypsin activity of the SprU was spectrophotometrically measured by the enzymatic hydrolysis of the artificial chromogenic substrate, N-${alpha}$-benzoyl-DL-arginine-p-nitroanilide, the S. lividans transformant with pWHM3-U gave 3 times higher activity than that of control. When the same recombinant plasmid was introduced into S. griseus, however, the gene dosage effect was not so significant, as in the cases of other genes encoding serine proteases, such as sprA, sprB, and sprD. Although two trypsins, SprU and SGT, have a high degree of homology, the pI values, the gene dosage effect in S. griseus, and the gene arrangement adjacent to the two genes are very different, suggesting that the biochemical and biological function of the SprU might be quite different from that of the SGT.

• Proceedings of the KWS Conference
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• 2006.05a
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• pp.310-312
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• 2006

Self-piercing riveting(SPR) is becoming an important joining technique for automotive application of various material sheets and shapes. Fatigue behavior of SPR conections needs to be investigated experimentally and numerically to predict SPR fatigue lives. The simulations of various SPR specimens (Coach-Peel specimen, Cross-Tension specimen, Tensile-Shear specimen, Pure-Shear specimen) are performed to predict the fatigue life of SPR connections under different material combinations. Finite element models of various SPR specimens are developed using a FEMFAT SPOT SPR pre-processor. The fatigue lives of SPR specimens are predicted using a FEMFAT 4.4e based on the liner finite element analysis.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.1
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• pp.147-154
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• 2009

This paper presents a grating~integrated SPR (Surface Plasmon Resonance) sensor chip for simple and inexpensive biomolecule detection. The grating-integrated SPR sensor chip has two sensing channels having a nano grating for SPR coupling. An external mirror is used for multi channel SPR sensing. The present sensor chip replaces bulky and expensive optical components, such as fiber-optic switches or special shaped prisms, resulting in a simple and inexpensive wavelength modulated multi-channel SPR sensing system. We fabricate a SPR sensor chip integrated with 835 nm-pitch gratings by a micromolding technique to reduce the fabrication cost. In the experimental characterization, the refractive index sensitivity of each sensing channel is measured as $321.8{\pm}8.1nm$/RI and $514.3{\pm}8.lnm$/RI, respectively. 0.5uM of the target biomolecule (streptavidin) was detected by a $1.13{\pm}0.16nm$ shift of the SPR dip in the 10%-biotinylated sample channel, while the SPR dip in the reference channel for environmental perturbation monitoring remained at the same position. From the experimental results, multi-channel biomolecule detection capability of the present grating-integrated SPR sensor chip has been verified. On the basis of the preliminary experiments, we successfully measured the binding reaction rate for the $2\;nM{\sim}200\;nM$ monoclonal-antibiotin, thus verifying biomolecule concentration detectability of the present SPR sensor chip. The binding reaction rates measured from the present SPR sensor chip agredd well with those from a commercialized SPR sensor.

• Journal of Sensor Science and Technology
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• v.22 no.2
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• pp.144-149
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• 2013

In this research, we developed a biosensor to detect lung cancer-specific biomarker using Anodic Aluminum Oxide (AAO) chip based on interference and nano surface plasmon resonance (nanoSPR). The nano-porous AAO chip was fabricated $2{\mu}m$ of pore-depth by two-step anodizing method for surface uniformity. NanoSPR has sensitivity to the refractive index (RI) of the surrounding medium and also provides simple and label-free detection when specific antibodies are immobilized to the Au-deposited surface of nano-porous AAO chip. To detect the lung cancer-specific biomarker, antibodies were immobilized on the surface of the chip by Self Assembled Monolayer (SAM) method. Since then lung cancer-specific biomarker was applied atop the antibodies immobilized layer. The specific reaction of the antigen-antibody contributed to the change in the refractive index that cause shift of resonance spectrum in the interference pattern. The Limit of Detection (LOD) was 1 fg/ml by using our nano-porous AAO biosensor chip.

• Journal of the Korean Society of Safety
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• v.30 no.4
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• pp.1-7
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• 2015

Self-piercing riveting (SPR) process is gaining popularity due to its many advantages. The SPR does not require a pre-drilled hole and has capability to join a wide range of similar or dissimilar materials and combinations of materials. This study investigated the fatigue strength of self-piercing rivet joint with aluminum alloy (Al-5052) and steel (SPCC) sheets. Static and fatigue tests on tensile-shear specimens were conducted. From the static strength aspect, the optimal punching force for the specimen with upper SPCC (U.S) sheet and lower aluminum alloy(L.A) sheets was 34 kN. During static test the specimens fractured in pull-out fracture mode due to influence of plastic deformation of joining area. There was a relationship between applied load amplitude $P_{amp}$ and number of cycles N ; $P_{amp}=19588N_f^{-0.211}$ and $P_{amp}=4885N_f^{-0.083}$ for U.S-L.A and U.A-L.S specimens, respectively. U.A-L.S fatigue specimens failed due to fretting crack initiation around the rivet neck between upper and lower sheets.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.635-638
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• 2003

For the acute assessment on biological toxicity of wastewater, surface plasmon resonance(SPR) based cell viability detection was performed using gold surface-confined cell as a result of adhesion-modifying chemicals. Escherichia coli O157:H7 (E. coli O157:H7) was investigated after exposure to EDTA. Cells were immobilized on gold coated slide glass for SPR analysis by the method of cross-linking carboxyl group on the bacterial surface with amine group of poly-L-lysine that had been coupled to the gold surface modified by a self-assembled monolayer of 11-mercaptounde canoic acid (11-(MUA)). Reflective intensity of each flow step was changed with respect to confect of ethylenediaminetetraacetic acid (EDTA) disodium salt and phosphate-buffered saline (PBS) solution. The proposed detection technique can be used for biological toxicity test.

• Proceedings of the Materials Research Society of Korea Conference
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• 2007.11a
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• pp.145.2-145.2
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• 2007

• Journal of the Korean Mathematical Society
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• v.60 no.2
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• pp.407-464
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• 2023

Let R be a commutative ring with identity. The structure theorem says that R is a PIR (resp., UFR, general ZPI-ring, π-ring) if and only if R is a finite direct product of PIDs (resp., UFDs, Dedekind domains, π-domains) and special primary rings. All of these four types of integral domains are Krull domains, so motivated by the structure theorem, we study the prime factorization of ideals in a ring that is a finite direct product of Krull domains and special primary rings. Such a ring will be called a general Krull ring. It is known that Krull domains can be characterized by the star operations v or t as follows: An integral domain R is a Krull domain if and only if every nonzero proper principal ideal of R can be written as a finite v- or t-product of prime ideals. However, this is not true for general Krull rings. In this paper, we introduce a new star operation u on R, so that R is a general Krull ring if and only if every proper principal ideal of R can be written as a finite u-product of prime ideals. We also study several ring-theoretic properties of general Krull rings including Kaplansky-type theorem, Mori-Nagata theorem, Nagata rings, and Noetherian property.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.630-633
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• 2003

Transfer of an electron from one site to another in a molecular or between molecules and/or electrodes is one of the most fundamental and ubiquitous processes in chemistry, biology and physics. In this study fusion proteins composed by green fluorescent protein(GFP) and cytochrome b562 were used in fabricating molecular array as an electron sensitizer and electron acceptor, Protein formation onto the substrate was performed by the self-assembly technique. The fusion protein film were analyzed using scanning probe microscope(SPM), Surface Plasmon Resornance(SPR) and hybrid STM/I-V. The results suggest that the proposed molecular photodiode can be used as a basic unit of the memory device.

• Proceedings of the Materials Research Society of Korea Conference
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• 2011.05a
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• pp.242.2-242.2
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• 2011

Molecularly imprinted polymer thin films were applied to develop a gas sensor based on the surface plasmon resonance phenomenon for small gaseous molecules such as toluene and xylene. The imprinted polymer films were synthesized via photo-polymerization method using various combination of templates, functional monomers and cross-linkers. The temperature of pre-polymerization solutions and the power of UV light were controlled for optimized performance of gas sensing. The morphology and porosity of the polymer films were controlled by varying the mixing ratios of the pre-polymerization solutions and confirmed by atomic force microscopy. By fitting the adsorption/desorption sensorgrams to conventional kinetic models, the effects of different templates and cross-linkers were interpreted in term of the structural differences of the polymer networks formed on the gold film. The sensitivity and selectivity of sensors were estimated for toluene and xylene, and also for humidity and other gaseous molecules such as formaldehyde and ammonia.