• Proceedings of the Korea Society of Poultry Science Conference
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• 2004.05a
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• pp.5-27
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• 2004

2003년 12월, 우리나라에서의 고병원성 가금인플루엔자(HPAI ： High Pathogenic Avian Influenza) 발생과 미국에서의 소해면상뇌증(BSE : Bovine Spongiform Encephalopathy) 발생이 매스컴을 통해 여과없이 발표되기 시작되자 닭고기와 쇠고기의 소비가 급격하게 줄어. 축산 생산기반마저 위협을 받는 위기에 처한 바가 있었다. (중략)

• Journal of Microbiology and Biotechnology
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• v.17 no.7
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• pp.1059-1070
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• 2007

Prion diseases, often called transmissible spongiform encephalopathies (TSEs), are infectious diseases that accompany neurological dysfunctions in many mammalian hosts. Prion diseases include Creutzfeldt-Jakob disease (CJD) in humans, bovine spongiform encephalopathy (BSE, "mad cow disease") in cattle, scrapie in sheep, and chronic wasting disease (CWD) in deer and elks. The cause of these fatal diseases is a proteinaceous pathogen termed prion that lacks functional nucleic acids. As demonstrated in the BSE outbreak and its transmission to humans, the onset of disease is not limited to a certain species but can be transmissible from one host species to another. Such a striking nature of prions has generated huge concerns in public health and attracted serious attention in the scientific communities. To date, the potential transmission of prions to humans via foodborne infection and iatrogenic routes has not been alleviated. Rather, the possible transmission of human to human or cervids to human aggravates the terrifying situation across the globe. In this review, basic features about prion diseases including clinical and pathological characteristics, etiology, and transmission of diseases are described. Based on recently accumulated evidences, the molecular and biochemical aspects of prions, with an emphasis on the molecular interactions involved in prion conversion that is critical during prion replication and pathogenesis, are also addressed.

• Journal of the korean veterinary medical association
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• v.32 no.5
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• pp.317-327
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• 1996

소의 해면형 뇌증(Bovine Spongiform Encephalpathy; BSE)은 우리나라에서의 발생보고는 없으나, 우리에게 그리 생소한 질병만은 아니다. 저자중 한사람인 진 영 화 박사는 일찌기 소 해면형 뇌증의 발견 초창기인 1987년도에 UNDP 가축위생강화계획에 의하여 영국 중앙수의연구소(CVL)에 파견되어 소 해면형 뇌증의 진단병리 연구에 참여한 바 있으며, 강 영 배 박사는 전국 각 시도 가축위생시험소장을 인솔하여 영국 중앙수의연구소를 3차례 다녀온 바 있다. 본편에서는, 1991년 해외출장 기간 중 영국정부를 통하여 입수한, 새로운 질병 즉 소의 해면형 뇌증(Bovine Spongiform Encephalpathy)에 대한 Video tape 자료중, 'BSE 이야기 - 모든 합리적인 대책방안(MAFF V710)'과 'BSE 임상증예 5예 보고(MAFF V659)'를 기본으로하여 사진 또는 영문자료와 함께 소개하고자 한다. BSE의 잠복기는 2년 이상으로 매우 길며, 3-5세의 성우에서 다발한다. 주요 임상증상은, 정신상태, 정서 및 운동의 이상으로 특징지어 지는데, 광증과 침울 등 행동이상을 나타내며, 특히 후지파행, 비틀거림, 미끄러짐, 넘어짐 등의 보행이상을 나타낸다. 초기의 관찰사항으로는, 출입구 통과나 착유실 입실을 거부하는 등 불안 상태를 흔히 나타내는 것이다. 건드리거나 소리에 민감하게 반응하는 지각과민증, 경증의 보행실조, 발로차기, 동물이나 관리자에 대한 공격적 자세, 그리고 공포자세가 흔히 수반된다. 임상소견은 발병기간 중 일관되게 유지되며, 개체별로는 수주일부터 수개월간 지속된다. 젖소에서는 산유량이 떨어지며, 6개월내지 1년 정도 경과후 100$\%$ 폐사되는 것이 특징이다. 치료시도는 전혀 효과가 없다.

• Food Science and Biotechnology
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• v.18 no.3
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• pp.782-787
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• 2009

In prion disease, spongiform neurodegeneration is preceded by earlier synaptic dysfunction. There is evidence that soluble N-ethylmaleimide sensitive factor attachment receptor (SNARE) complex formation is reduced in scrapie-infected in vivo models, which might explain this synaptic dysfunction because SNARE complex plays a crucial role in neuroexocytosis. In the present study, however, it is shown that prion protein (PrP) does not interfere with SNARE complex formation of 3 SNARE proteins: syntaxin 1a, SNAP-25, and synaptobrevin. Sodium dodecyl sulfate-resistant complex formation, SNAREdriven membrane fusion, and neuroexocytosis of PC12 cells were not altered by PrP. Thus, PrP does not alter synaptic function by directly interfering with SNARE complex formation.

• Korean Journal of Veterinary Service
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• v.32 no.4
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• pp.307-314
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• 2009

The aim of the present study was to investigate whether Bovine Spongiform Encephalopathy (BSE) is present in this country and to analyze the Global BSE Risk (GBR) status in Bangladesh. A total of 2,000 brain samples were collected from cattle older than 30 months of age, slaughtered for human consumption in the district slaughter houses from 2005 to 2006. The brainstem (obex), Pyriform lobe, cerebrum and cerebellum were subjected to histopathological study. Samples that showed some nonspecific lesions were subjected to immunohistochemistry and only brain stem to ELISA for the detection of abnormal prion protein $PrP^{sc}$. In passive surveillance, annual overall diseases of cattle, buffalo, sheep and goats in Bangladesh were collected from Department of Livestock Services (DLS), Dhaka to investigate the occurrences of neurological diseases. Import related data were collected from "National Export Promotion Bureau" Kawran Bazar, Bangladesh Bank and DLS to analyze the importing products of animal origin (cattle, buffalo, sheep and goats) from different countries to find whether or not the imported products posed any risk for the BSE. In an actire surveillance conducted in slaughter house, histopathologically BSE specific lesions were not detected in any of the brain samples, but other nonspecific lesions were observed. No $PrP^{sc}$ was detected from the samples by immunohistochemistry and ELISA. DLS report also supported the absence of BSE in cattle and buffalo and scrapie in sheep and goats in Bangladesh. It was also clearly recorded that Bangladesh imported livestock products from countries in GBR level I and II but not from countries in GBR level III and IV. From this study it apparently seems that BSE is not currently present in the indigenous animals in Bangladesh and poses no or negligible risk to human and animal health.

• Korean Journal of Radiology
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• v.21 no.6
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• pp.764-772
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• 2020

Objective: Laser ablation is a therapeutic modality used to reduce the volume of large benign thyroid nodules. Unsatisfactory reduction and regrowth are observed in some treated nodules. The aim of the study was to evaluate the long-term outcomes of laser treatment for solid nodules during a 5-year follow-up period, the regrowth rate, and the predictive risk factors of nodule regrowth. Materials and Methods: We retrospectively evaluated patients with benign, solid, cold thyroid nodules who underwent laser ablation and were followed-up for 5 years. According to the selection criteria, 104 patients were included (median baseline nodule volume, 12.5 mL [25.0-75.0%, 8-18 mL]; median energy delivered, 481.5 J/mL [25.0-75.0%, 370-620 J/mL]). Nodule volume, thyroid function test results, and ultrasound were evaluated at baseline and then annually after the procedure. Results: Of 104 patients, 31 patients (29.8%) had a 12-month volume reduction ratio (VRR) < 50.0% and 39 (37.5%) experienced nodule regrowth. Of these 39 patients, 17 (43.6%) underwent surgery and 14 (35.9%) underwent a second laser treatment. The rate of nodule regrowth was inversely related to the 12-month VRR, i.e., the lower the 12-month VRR, the higher the risk of regrowth (p < 0.001). The mean time for nodule regrowth was 33.5 ± 16.6 months. The 12-month VRR was directly related to time to regrowth, i.e., the lower the 12-month VRR, the shorter the time to regrowth (p < 0.001; R² = 0.3516). Non-spongiform composition increased the risk of regrowth with an odds ratio of 4.3 (95% confidence interval [CI] 1.8-10.2; p < 0.001); 12-month VRR < 50.0% increased the risk of regrowth with an odds ratio of 11.7 (95% CI 4.2-32.2; p < 0.001). Conclusion: The VRR of thyroid nodules subjected to similar amounts of laser energy varies widely and depends on the nodule composition; non-spongiform nodules are reduced to a lesser extent and regrow more frequently than spongiform nodules. A 12-month VRR < 50.0% is a predictive risk factor for regrowth and correlates with the time to regrowth.

• Journal of the korean veterinary medical association
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• v.32 no.3
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• pp.137-142
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• 1996

최근 소의 광우병 발생보고가 영국에서 16만여두 그리고 EU 14게국에서 약 150여 마리에 이르므로써 우산업분야에 미치는 심각한 경제적 여파와 인체에서의 Creutzfeldt-Jakob병과 어떤 연관성 논란으로 공중보건상 심각한 논쟁이 일고 있다. 본회 학술홍보 위원회에서는 이 질병에 관한 현재까지의 알려진 일반적인 지식을 정리하여 회원들에게 우선 소개하고자한다. 이 질병에 관한 보다 정리된 종합적인 내용은 수의과학연구소 해외전염병과에서 정리하여 다음호에 특집으로 소개할 것입니다.

• Microbiology and Biotechnology Letters
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• v.43 no.2
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• pp.91-96
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• 2015

Prions are proteinaceous infectious particles that cause neurodegenerative diseases, such as scrapie in sheep, bovine spongiform encephalopathy in cattle and Creutzfeldt-Jakob disease (CJD) in humans. Although the detailed process, regarding the abnormal conversion of prion proteins (PrP), remains to be fully elucidated, a number of environmental factors appear to affect the formation of misfolded PrP, termed PrP^Sc. Because oceanic algae contain mycosporine-like amino acids (MAAs), which exhibit cellular defensive activities under a variety of stress conditions, we investigated the level of PrP^Sc in prion-infected neuroblastoma cells using mycosporine-glycine, porphyra-334 and shinorine. When judged by the level of protease-resistant PrP^Sc in western blots, porphyra-334 and shinorine increased the level of PrP^Sc in cells, but mycosporine-glycine did not. The current results indicate that the MAAs tested in this study enhance the formation of PrP^Sc.

• Journal of Animal Science and Technology
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• v.51 no.6
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• pp.453-458
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• 2009

Prion protein (PRNP) is known to be a causative protein for transmissible spongiform encephalopathy (TSE), a disease occurring in human and animals. Previous results indicate that the genetic variability can affect the resistance and susceptibility of goat scrapie and can give the guideline for reducing the risk of this disease. Until now, 35 single nucleotide polymorphisms (SNPs) were identified in goat PRNP gene from many countries such as Great Britain, Italy, United States of America and Asian countries etc. In this study, SNPs in PRNP gene have been investigated to research the PRNP variations and their possible TSE risks in 60 Korean native goats. Based on the sequencing results, we identified four SNPs and three of those polymorphisms (G126A, C414T and C718T) were synonymous and the A428G polymorphism was non-synonymous which changes the amino acid histidine to arginine. Previously, all of these four SNPs were identified in Asian native goats. Specifically, five polymorphisms were identified in Asian native goats and two of them (G126A and C414T) were silent mutations, and the other SNPs (T304G, A428G and T718C) caused amino acid changes (W102G, H143R and S240P). Comparing with SNP results from other breeds, this study is an initial step to understand resistance and susceptibility of this disease in Korean native goats.

• The Korean Journal of Food And Nutrition
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• v.24 no.2
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• pp.166-172
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• 2011

This study was conducted to assess food safety knowledge of college students according to mass-media reports. To accomplish this, a survey was conducted to investigate the reliability, of primary mass-media sources on Bovine Spongiform Encephalopathy(BSE) as well as the preferences of college students. The most common sources of media consumed by male and female students were terrestrial television(TV)(60%), the internet(21%), and newspapers(11%) and terrestrial TV(60%), newspapers (18%), and the internet(17%), respectively. The preferences for media of major and non-major students were terrestrial TV(64%), newspapers(17%), and the internet(14%) and terrestrial TV(51%), the internet(22%), and newspapers(19%), respectively. The reliability of media as viewed by male students were as follows: terrestrial TV(53%) and the internet(40%), whereas for female students, these values were terrestrial TV(55%) and the internet(37%). The reliability of media as viewed by major and non-major students was as follows: terrestrial TV(67%) and the internet(28%), and terrestrial TV(44%) and the internet(44%), respectively. In the case of male students, the primary sources for information regarding BSE were terrestrial TV(54%) and the internet(38%), whereas for female students, the primary sources were terrestrial TV(57%) and internet (37%). In the case of major students, the primary sources for information regarding BSE were terrestrial TV(52%) and the Internet(40%), and for non-major students, the internet(47%) and terrestrial TV(39%). Both male and female students were found to have less knowledge regarding prions, SRM(Specified Risk Materials), MM(methionine-methionine) type genes, and cow above 30 months associated with BSE compared to the other factors associated with this disease. The BSE-related knowledge held by major and non-major students was revealed unknown more than the majority of responses. Based on these results, greater effort should be made to provide meaningful information to improve the food-safety knowledge of college students according to mass-media.