• Journal of Ecology and Environment
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• 제35권4호
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• pp.343-349
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• 2012

The use of growth medium is often recommended milkweed seedlings to grow and develop after emergence, and it is affected by growth medium and local habitat conditions. The effects of growth medium and partial shade on early growth of milkweed under drought stress (Calotropis procera L.) were studied in a field experiment. A split-split plot experimental design with three replications was carried out in the nursery. The main treatment plot was divided into two levels of shade; (no shading and partial shading). Sub treatment plot1 included growth medium at four levels (G1 = clay [suitable for milkweed growth], G2 = clay + sand, G3 = clay + perlite, G4 = clay + perlite + sand) and sub treatment plot2 included drought (irrigation intervals) at six levels (D1 = 2 [control], D2 = 4, D3 = 6, D4 = 8, D5 = 10, and D6 = 12 days per for three month). The results showed that drought stress significantly decreased emergence percentage, shoot length, shoot dry weight (SDW1), root dry weight (RDW), seedling dry weight (SDW2) and vigor index (VI). The use of growth medium increased all seedling characteristics. The G3 (clay + perlite) growth medium showed the highest performance, especially in terms of emergence percentage and seedling dry weight. Partial shade improved shoot length, shoot dry weight, and vigor index. Our results showed that the best treatment for high-vigor milkweed seedlings under drought stress was G3 (clay + perlite) growth medium and partial shade.

• 한국작물학회지
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• 제51권1호
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• pp.1-13
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• 2006

Response of grain yield and milled-rice protein content to nitrogen topdress (N) timing at panicle initiation stage (PIS) is critical for quantifying real-time N requirement for target grain yield and milled-rice protein content. Two split-split-plot experiments with three replications, one in 2004 and the other in 2005, were conducted in Experimental Farm, Seoul National University, Suwon, Korea. The experiments included three N rates at tillering stage (TS), three N timing treatments at panicle initiation stage (PIS) and two rice cultivars. The N rates at TS, N timing at PIS, and rice cultivars were randomly assigned to main plot, sub plot, and sub-sub plot, respectively. Results showed that the delayed N application at PIS reduced grain yield in 2004 and increased milled-rice protein content in both years significantly at 0.05 probability level. The calculated optimum N timing at PIS from pooled data by N rates and rice cultivars in two years was at 28 days before heading (DBH). However, real-time of N timing at PIS was dependent on plant growth and N status around PIS that in turn was dependent on applied N rates at TS. The optimum N timing at PIS was at 30 DBH for no N treatments at TS while at 27 DBH for 3.6 and 7.2 kg N/10a treatments and at 27 and 29 DBH for Hwaseongbyeo and Daeanbyeo, respectively. In general, earlier applied N at PIS resulted in lower milled-rice protein content but the highest grain yield was expected to be obtained when N topdress at PIS was applied at the time when shoot N concentration started to drop below about 23 mg/g due to dilution effect after transplanting. In conclusion, the results of our experiments imply that the currently recommended N topdress time (24DBH) at PIS in Korea should be reconsidered for the higher grain yield and the better quality of rice.

• 한국연초학회지
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• 제10권2호
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• pp.93-98
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• 1988

This study was conducted to obtain basic information for varietal difference of leaf breakdown in field of flue-cured tobacco at Taegu Experiment Station, Korea Ginseng & Tobacco Research Institute in 1987. The experiment was designed in randomized block with 3 replications, data ware analysed as Split split-plot design. Main plots were varieties, sub-plots were leaf positions, 4, 5 and 6th from bottom, and each sub-plot was divided into 3 parts with distance of midrib, 7 10 and 13em from stalk. Four varieties, NC 95, NC 2326, NC 82 and BY 4 were transplanted in 15 April, and measured the weight of leaf breakdown by artificially weighted in 5 June. The results obtained are as follows : 1. Weights of leaf breakdown according to leaf position, NC 95 were lower as 358-5799 than those of other varieties as 555-597g, were not significantly different regardless of varieties. 2. Weights of leaf breakdown in relation to distance of midrib from stack, NC 95 were lower as 309-419g than those of other varieties as 472-710g. 3. Weights of leaf breakdown were significantly different according to distance of midrib from stalk, and not significantly different according to leaf position under the same distance of midrib from stalk regardless of varieties.

• Journal of Plant Biology
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• 제30권2호
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• pp.135-149
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• 1987

An experiment was made in order to analyze the growth characteristics and productivity of Zoysia japonica under control, mowing and TIBA treatment conditions at the experimental farm of Kyung Hee University. The field was planned by the split plot design method and each treatment was given to two plots (40$\times$40 and 100$\times$100 mm) and was replicated three times. Each plot was 9$m^2$ for Zoysia japonica. The sampling of each plot was taken once a week after sowing. In order to know the dry matter of total standing crops, each organ of plants was kept at 9$0^{\circ}C$ and weighed. Total leaf area of a plant was measured by drawing method. The author adopted the growth analysis of English School. Holocellulose analysis by sodium chlorite method was made. The increasing rate of LAI was high in all plots between 10th and 12th week after sowing and high growth rate of assimilatory organ was observed in low density area of TIBA plot. Between 10th and 12th week after sowing all the experimental plots showed high increasing rate of standing crop and in the process of growth each density area of TIBA plot recorded high increasing rate. In all the plots F/C ratio and RGR were high when the growth rate of assimilatory organ was increasing cosiderably, and the higher the planting density was, the higher F/C ratio was recorded. In all the plots NAR recorded maximum rate before the growth period to show a high increase of RGR. In the process of the growth TIBA plot showed high icnreasing rate of NAR. CGR showed high value in high density areas of all the plots and reached its maximum in the 13th week after sowing. Compared to the other experimental areas the low planting density areas of control plot and TIBA plot showed considerable earlier lignification indicating low Holocellulose content in the growth process.

• Animal Bioscience
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• 제35권1호
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• pp.39-46
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• 2022

Objective: The fermentation profile and silage quality of 3 Italian ryegrass (Lolium multiflorum Lam.) cultivars (cvs. Devis, Hellen, and Trinova) treated with 5 nitrogen doses (0, 50, 100, 150, and 200 kg/ha) were evaluated. Methods: The experiment was laid out in split plot in randomized complete block design with three replications. Annual ryegrass cultivars used in this study have been commonly grown in Turkey. Nitrogen doses were set in main plot and cultivars in split plot in the field. Plants were harvested at full-flowering stage with dry matter content about 220 g/kg for first cutting and 260 g/kg for second cutting. Harvested plants were chopped theoretically into 2 to 3 cm lengths for ensiling. Chopped fresh materials were ensilaged by compressing in 2 L plastic jars about 3±0.1 kg. Results: Effects of N doses on dry matter, neutral detergent fiber, acid detergent fiber, dry matter digestibility, relative feed value, crude protein, pH, ammonia nitrogen, lactic acid, acetic acid, and lactic acid/acetic acid were statistically significant while water soluble carbohydrate, ash and organic matter were not statistically different. Ammonia nitrogen, crude protein, ash, organic matter, lactic acid, and lactic acid/acetic acid were affected by cultivars, but the other parameters were not. Increasing nitrogen applications positively affected the chemical composition of annual ryegrass silage. The significant increase in protein content was remarkable, however, silage fermentation properties were adversely affected by the increasing nitrogen dose. Conclusion: It can be recommended 150 kg/ha nitrogen dose for annual ryegrass harvested at full blooming stage. Even though the silage fermentation properties of the used cultivars were similar, cv. Devis gave better results than the others in terms of silage pH and relative feed value.

• 한국초지조사료학회지
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• 제12권2호
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• pp.98-103
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• 1992

With a purpose of finding out the effects of cattle slurry application on thc nutrient contents of herbages and energy productivity in grassland, a field experiment was conducted with two times and five levels of cattle slurry application. The experimental field was designed in a split-plot with threc replications, and lasted from September, 1986 to October, 1990 at the Livestock Experiment Station in Suweon, The results obtained are summarized as follows: 1. The net energy(NEL, StE and TDN) contents of grasses in the plots with cattle slurry application just after cutting were slightly higher when compared to manuring 15 days after cutting. but DCP content has reverse trend. 2. Average net energy(NEL. StE and TDN) and DCP production of the plot with cattle slurry application 15 days after cutting were insignificantly increased by 26 % than those of manuring just after cutting. The energy and DCP yields in the plot with cattle slurry and with slurry + mineral fertilizer were decreased by 21 -27 % and 8- 11 %, respectively than those of the plot with single dressing of mineral fertilizer. 3. Mineral contents of grasses were not significant difference among the treatments of cattle slurry. but considering nutrient balance of animals, application of cattle slurry of 30-$60m^3$/ha with mineral fertilizer was profitable as compared to the other treatments.

• 한국초지조사료학회지
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• 제17권4호
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• pp.371-378
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• 1997

The purpose of this research was to investigate the effect of P fertilization and cutting fkquency without N fertilizer on nutritive value and nitrogen fixation activity of alfalfa. A field experiment was conducted on the experimental farm of Kongju National University at Yesan from 1994 to 1996. The experimental design was a split plot design with two P levels as main plot and two cutting frequencies as the sub-plot. The results obtained are summarized as follows. Available phosphate, exchangeable Ca and Na in the soil were slightly increased with P fertilizer and basal treatment of lime. Average DM yield was 1.5 ton and 1.4 ton in second and third year. There was no significant difference within P level and cutting kquency. The first cut showed higest CP value as cutting times increased the value tend to be lowered. Crude protein content in 3 and 4 cutting regimes were from 16.3 to 16.6% 17.4 to 17.9% respectively, but no significant effect were found among the main and sub-plot. ADF and NDF in 1995 were ranged from 38.2 to 39.9, 45.1 to 58.1% respectively. ADF was 2% more higher in 1996 compared to previous year and NDF was slightly higher in 1996. There was significant difference of ADF between main plot in 1995. .P, Ca, K and Mg of 200kg P level with 3 times cutting treatment in 1995 and 1996 were 0.32 and 0.23, 0.95 and 0.84, 3.24 and 2.87, and 0.36 and 0.26. Significant difference of K and Mg between cutting frequency was observed in 1995. Nitrogen fixing activity of 400kg P fertilizer plots were higher than 200kg P level and 3 times cutting plot also showed higher acetylene reduction activity than that of 4 cutting per year. Phosphate level and cutting frequency treatment of did not significantly affect nitrogen fixation activity.

• 품질경영학회지
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• 제33권3호
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• pp.156-160
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• 2005

기존의 표준공법과 신공법간의 품질특성치의 평균에 차이가 있는지를 알아보기 위한 가장 실용적인 실험 설계는 표준공법으로 진행되고 있는 공정을 신공법으로 바꾼 후에 연속적으로 생산해서 실험 자료를 얻는 것이다. 이 실험 자료로부터 평균 수율의 차이가 있는 지를 검정하기 위해서는 표준공법으로 연속해서 생산된 제품들의 수율에 관한 정보를 추가적으로 필요로 한다. 표준공법으로 연속해서 생산된 제품들의 수율에 관한 충분한 최근의 자료가 없으면 분할법에 의한 실험설계가 실용적인 설계이다. 분할법 I은 각 단계의 실험을 축차적으로도 실시할 수 있다는 장점이 있다. 단계 1에서는 표준공법과 신공법의 실시순서를 랜덤하게 정한다. 그다음에 정해진 방법에 따라서 연속해서 실험을 r회 실시한다. 단계 2,..., rep에서도 동일한 방법으로 실험을 실시한다. 분할법 II는 모든 실험을 한번에 실시하는 설계로, 두 개의 공정조건의 변경이 제한적으로 가능하다면 먼저 rep개의 반복을 갖는 각 공법을 2*rep개의 일차단위에 랜덤하게 배치하고, 일차단위에 랜덤하게 배치된 공정조건을 고정시키고 실험을 r회 반복한다. 분할법에서 일반적으로 공정조건을 변경하는데 비용과 시간이 많이 들어서 rep를 적게 하는 효율적인 실험의 반복수 ${\gamma}^*$을 결정하는 것이 엔지니어들의 관심사항이다. 분할법 I이나 분할법 II에서의 효율적인 실험의 반복수는 ${\gamma}^*$=6이고, 차선책은 ${\gamma}^*$=4이다.

• Communications for Statistical Applications and Methods
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• 제4권2호
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• pp.581-588
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• 1997

Univariate F test is based on the multisample sphericity assumption. Robustness for tests of a main effect of the within-subjects factor was investigated when the assumptions of the onmibus F tests are violated in a split-plot design with one between-subjects factor using untrimmed data and trimmed data. The results indicate that when sample sizes are unbalanced and dispersion matrices are heterogeneous, the CIGA and the $CIGA_T$ tests better control Type I error rates than do the $F_T$test and the$\widetilde{\xi_T}$test.

• Communications for Statistical Applications and Methods
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• 제4권3호
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• pp.775-785
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• 1997

Robustness rates for repeated measures pairwise multiple comparison procedures were investigated in a split plot design with one between- and one within-subjects factor using untrimmed and trimmed data. Five factors were manipulated in the study: distribution, sphericity, variance-covariance heteroscedasticity, total sample size, and sample size ratio. The Welsch test (W) and the Welsch test on trimmed data $(W_{RT})$ performed better than the other procedures, but had a liberal tendency. The trimmed difference score Bonferroni Procedure $(B_{DT})$ was a good choice in some conditions.