• 식품보건융합연구
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• 제4권4호
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• pp.18-25
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• 2018

Proteins play a key role in many functions such as metabolic activity, differentiation, as cargos and cell fate regulators. It is necessary to know about the markers involved in male fertility in order to develop remedies for the treatment of male infertility. But, the role of the proteins is not limited to particular function in the biological systems. Some of the proteins act as ion channels such as catsper and proteins like Nanos acts as a translational repressor in germ cells and expressed in prenatal period whose role in male fertility is uncertain. Rbm5 is a pre mRNA splicing factor necessary for sperm differentiation whose loss of function results deficit in sperm production. DEFB114 is a beta defensin family protein necessary for sperm motility in LPS challenged mice where as TEX 101 is a plasma membrane specific germ cell protein whose function is not clearly known u to now. Gpr56 is another adhesion protein whose null mutation leads to arrest of production of pups in rats. Amyloid precursor protein role in Alzheimer's disease is already known but it plays an important role in male fertility also but its function is uncertain and has to be considered while targeting APP during the treatment of Alzheimer's disease. The study on amyloid precursor protein in male fertility is a novel thing but requires further study in correlation to alzheimer's disease.

• Steel and Composite Structures
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• 제38권4호
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• pp.365-380
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• 2021

Although traditional steel-concrete composite beams have excellent structural characteristics, it cannot meet the requirement of quick assembly and repair in the engineering. This paper presents a study on static behavior of bolt connected steel-concrete composite beam without post-cast zone. A three-dimensional finite element model was developed with its accuracy and reliability validated by available experimental results. The analysis results show that in the normal service stage, the bolt is basically in the state of unidirectional stress with the loss of pretightening can be ignored. Parametric studies are presented to quantify the effects of the post-cast zone, size and position of splicing gap on the behavior of the beam. Based on the studies, suggested size of gap and installation order were proposed. It is also confirmed that optimized concrete slab in mid-span can reduce the requirement of construction accuracy.

• BMB Reports
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• 제54권7호
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• pp.335-343
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• 2021

Cancer stem cells (CSCs) are a subpopulation of cancer that can self-renew and differentiate into large tumor masses. Evidence accumulated to date shows that CSCs affect tumor proliferation, recurrence, and resistance to chemotherapy. Recent studies have shown that, like stem cells, CSCs maintain cells with self-renewal capacity by means of asymmetric division and promote cell proliferation by means of symmetric division. This cell division is regulated by fate determinants, such as the NUMB protein, which recently has also been confirmed as a tumor suppressor. Loss of NUMB expression leads to uncontrolled proliferation and amplification of the CSC pool, which promotes the Notch signaling pathway and reduces the expression of the p53 protein. NUMB genes are alternatively spliced to produce six functionally distinct isoforms. An interesting recent discovery is that the protein NUMB isoform produced by alternative splicing of NUMB plays an important role in promoting carcinogenesis. In this review, we summarize the known functions of NUMB and NUMB isoforms related to the proliferation and generation of CSCs.

• Molecules and Cells
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• 제43권7호
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• pp.662-670
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• 2020

We have investigated the involvement of the pre-mRNA processing factor 4B (PRP4) kinase domain in mediating drug resistance. HCT116 cells were treated with curcumin, and apoptosis was assessed based on flow cytometry and the generation of reactive oxygen species (ROS). Cells were then transfected with PRP4 or pre-mRNA-processing-splicing factor 8 (PRP8), and drug resistance was analyzed both in vitro and in vivo. Furthermore, we deleted the kinase domain in PRP4 using Gateway™ technology. Curcumin induced cell death through the production of ROS and decreased the activation of survival signals, but PRP4 overexpression reversed the curcumin-induced oxidative stress and apoptosis. PRP8 failed to reverse the curcumin-induced apoptosis in the HCT116 colon cancer cell line. In xenograft mouse model experiments, curcumin effectively reduced tumour size whereas PRP4 conferred resistance to curcumin, which was evident from increasing tumour size, while PRP8 failed to regulate the curcumin action. PRP4 overexpression altered the morphology, rearranged the actin cytoskeleton, triggered epithelial-mesenchymal transition (EMT), and decreased the invasiveness of HCT116 cells. The loss of E-cadherin, a hallmark of EMT, was observed in HCT116 cells overexpressing PRP4. Moreover, we observed that the EMT-inducing potential of PRP4 was aborted after the deletion of its kinase domain. Collectively, our investigations suggest that the PRP4 kinase domain is responsible for promoting drug resistance to curcumin by inducing EMT. Further evaluation of PRP4-induced inhibition of cell death and PRP4 kinase domain interactions with various other proteins might lead to the development of novel approaches for overcoming drug resistance in patients with colon cancer.

• 한국가축번식학회지
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• 제20권4호
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• pp.453-458
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• 1997

형질전환 동물의 후손에서 transgene은 멘델의 법칙에 따라 유전된다고 일반적으로 인식되어져 왔다. 따라서 본 연구에서는 transgene이 이러한 인식과 일치하는지를 여러 세대를 통하여 확인하고 후손에서 어떻게 유전되는지를 연구하기 위하여 형질전환 생쥐를 생산하여 본 연구의 모델로 삼았다. 수정된 생쥐의 embryo에 DNA를 microinjection하는 방법으로 MMTV-LTR (long terminal repeat), bovine ($\alpha$s1-casein cDNA, 그리고 SV 40 splicing과 polyadenylation site 등의 sequence를 포함한 3.0Kb의 DNA가 주입되었다. 여기에서 태어난 새끼는 dot blot과 Southern blot에 의하여 transgene의 존재여부가 확인되어 founder line이 만들어졌다. 그들의 자손은 PCR에 의해서 transgene이 유전되는지를 확인하였다. F0의 72마리 새끼중에서 4마리의 Founder가 transgene을 가지고 있었다(5.6%). F0에서 F1으로의 유전(transmission)은 각각 33.3, 7.7, 0, 62.5%이었다. Transgene은 F1에서 F2로 각각 63.6, 5.9, 68.8% 유전되었고, F2에서 F3로 각각 85.7, 0, 88.2% 유전되었다. 따라서 본 연구 모델에 의하면 transgene은 멘델의 법칙을 따르는 경우와 deletion이 되는 경우로 각각 관찰되었다.

• 마이크로전자및패키징학회지
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• 제12권4호통권37호
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• pp.323-330
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• 2005

저밀도 파장분할 방식 (CWDM)을 사용하는 광중계기와 광전송시스템에 사용할 수 있는 PIN PD (Positive Intrinsic Negative Photo Diode)를 제작하고 특성을 알아보았다. 특별히 제작된 CWDM 필터를 PD 패키지에 포함하여 일체형으로 제작하여 기존에 별도로 연결하여 사용하던 방법에 비해 작업성과성능 그리고 가격면에서 우수함을 보였다. 일체형 저밀도 파장분할 PD를 제작하기 위해서 CWDM 필터 조립 단계, PD 패키징 단계, 완제품 최종 조립 및 측정 단계의 3단계 과정을 수행하였다. 제작된 PD는 0.5 dB 대역폭이 17 nm, 투과 단자의 인접채널의 고립도는 60 dB 이상으로 측정되었고, 반사 단자의 고립도는 20 dB 이상으로 측정되었다. 무선주파수 특성을 위해 IMD3를 측정결과 63dBc 이상이었으며 PD의 응답도는 제작 샘플 23개중 20개가 0.9A/W 이상이었다. 일체형으로 제작함으로써 전체적인 삽입손실이 0.4-0.7 dB 정도 줄었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권7호
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• pp.1017-1021
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• 2005

Myostatin is a negative regulator of skeletal muscle growth and a loss of functional myostatin protein increases muscle hypertrophy and hyperplasia in cattle. The present study was conducted to investigate whether maternal passive immunization against myostatin would improve growth performance in chickens. A complete broiler myostatin cDNA was cloned and it was expressed into two transcripts as 1,128 bp and 985 bp by alternative splicing. A conjugated mature myostatin (350 bp) was used to induce autoimmunization and maternal passively immunized chickens was used for the experiment. It was confirmed that there was a maternal passive immunization against myostatin at zero weeks of age, but its effect was reduced by 6 weeks of age. The auto-immunized groups showed smaller body weights than those of control group during the growing period and the difference was getting bigger with time until 6 weeks of age. These results suggest that passive autoimmunization against myostatin used in this study is not potent enough to stimulate growth performance in chickens.

• 한국광학회지
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• 제4권4호
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• pp.420-427
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• 1993

융착 접속 기술을 이용하여 다중모우드 광섬유와 단일 모우드 광섬유내에 유전체 박막 거울을 제작하였다. $45{\circ}$ 유전체 거울이 내장된 광섬유는 극소형이며, 광학적인 손실이 매우 작고(1.3 ${\mu}m$에서, 다중 모우드 광섬유의 경우 0.2dB, 단일 모우드 광섬유의 경우 0.5dB), 기계적 강도가 우수한 결합기로 사용될 수 있다. 반사율은 파장에 따라 변화하며, 편광에 매우 민감하였다. 백색광을 사용하여 유전체 거울로부터 반사되는 출력 파워를 원거리 스캔하며 측정하였을 때 출력 빔의 모양은 거의 원형 대칭으로써 최대 파워의 5%에서 측정된 종횡비는 1.09이었다. 다이오우드 레이저 광원을 사용하여 측정한 다중모우드 광섬유 결합기의 광분파율은 종래의 FBT(Fused Biconical Taper) 결합기보다 입력 광신호의 결합 조건에 따른 변화가 훨씬 적어서 사용하는 광통신 시스템의 모우드 잡음에 덜 민감하다. 광섬유 축에 수직하게 증착된 다층 유전체 거울들의 반사율 스펙트럼 특성을 측정하였으며, 행렬 해석법을 사용하여 실험 결과를 분석, 고찰하였다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2002년도 창립10주년기념 및 국립독성연구원 의약품동등성평가부서 신설기념 국재학술대회:생물학적 동등성과 의약품 개발 전략을 위한 국제심포지움
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• pp.113-113
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• 2002

Phylogenetically conserved Bcl-2 family proteins play a pivotal role in the regulation of apoptosis from virus to human. Members of the Bcl-2 family consist of antiapoptotic proteins such as Bcl-2, Bcl-xL, and Bcl-w, and proapoptotic proteins such as BAD, Bax, BOD, and Bok. It has been proposed that anti- and proapoptotic Bcl-2 proteins regulate cell death by binding to each other and forming heterodimers. A delicate balance between anti- and proapoptotic Bcl-2 family members exists in each cell and the relative concentration of these two groups of proteins determines whether the cell survives or undergoes apoptosis. Mcl-1 (Myeloid cell :leukemia-1) is a member of the Bcl-2 family proteins and was originally cloned as a differentiation-induced early gene that was activated in the human myeloblastic leukemia cell line, ML-1 . Mcl-1 is expressed in a wide variety of tissues and cells including neoplastic ones. We recently identified a short splicing variant of Mcl-1 short (Mcl-IS) and designated the known Mcl-1 as Mcl-1 long (Mcl-lL). Mcl-lL protein exhibits antiapoptotic activity and possesses the BH (Bcl-2 homology) 1, BH2, BH3, and transmembrane (TM) domains found in related Bcl-2 proteins. In contrast, Mcl-1 S is a BH3 domain-only proapoptotic protein that heterodimerizes with Mcl-lL. Although both Mc1-lL and Mcl-lS proteins contain BH domains fecund in other Bcl-2 family proteins, they are distinguished by their unusually long N-terminal sequences containing PEST (proline, glutamic acid, serine, and threonine) motifs, four pairs of arginine residues, and alanine- and glycine-rich regions. In addition, the expression pattern of Mcl-1 protein is different from that of Bcl-2 suggesting a unique role (or Mcl-1 in apoptosis regulation. Tankyrasel (TRF1-interacting, ankyrin-related ADP-related polymerasel) was originally isolated based on its binding to TRF 1 (telomeric repeat binding factor-1) and contains the sterile alpha motif (SAM) module, 24 ankyrin (ANK) repeats, and the catalytic domain of poly(adenosine diphosphate-ribose) polymerase (PARP). Previous studies showed that tankyrasel promotes telomere elongation in human cells presumably by inhibiting TRFI though its poly(ADP-ribosyl)action by tankyrasel . In addition, tankyrasel poly(ADP-ribosyl)ates Insulin-responsive amino peptidase (IRAP), a resident protein of GLUT4 vesicles, and insulin stimulates the PARP activity of tankyrase1 through its phosphorylation by mitogen-activated protein kinase (MAPK). ADP-ribosylation is a posttranslational modification that usually results in a loss of protein activity presumably by enhancing protein turnover. However, little information is available regarding the physiological function(s) of tankyrase1 other than as a PARP enzyme. In the present study, we found tankyrasel as a specific-binding protein of Mcl-1 Overexpression of tankyrasel led to the inhibition of both the apoptotic activity of Mel-lS and the survival action of Mcl-lL in mammalian cells. Unlike other known tankyrasel-interacting proteins, tankyrasel did not poly(ADP-ribosyl)ate either of the Mcl-1 proteins despite its ability to decrease Mcl-1 proteins expression following coexpression. Therefore, this study provides a novel mechanism to regulate Mcl-1-modulated apoptosis in which tankyrasel downregulates the expression of Mcl-1 proteins without the involvement of its ADP-ribosylation activity.

• 한국광학회지
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• 제32권2호
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• pp.49-54
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• 2021

본 논문에서는 고출력 광섬유 레이저의 핵심 부품인 대구경 광섬유 엔드캡을 제작하는 장비를 설계 및 제작하였으며, 제작장비를 이용하여 대구경 광섬유 엔드캡을 제작하였다. 대구경 광섬유 엔드캡 제작장비는 레이저 광을 조사하여 접속 열원으로 사용하기 위한 CO2 레이저 광원부, 대구경 광섬유와 엔드캡의 위치를 이송하기 위한 정밀 스테이지 조립체, 스테이지 조립체와 연동되어 융착 시 정렬에 사용되는 비전 시스템으로 구성되어 있다. 레이저 광원의 출력은 스테이지 조립체와 연동되어 출력을 제어하며, 비전 시스템으로 대구경 편광유지 광섬유의 편광축을 정렬할 수 있도록 제작되었다. 자체 제작한 장비를 이용하여 클래드 직경이 400 ㎛인 대구경 편광유지 광섬유와 10(W)×5(D)×2(H) ㎣의 엔드캡을 레이저 융착하여 대구경 광섬유 엔드캡을 제작하였다. 제작된 대구경 광섬유 엔드캡의 신호광 삽입손실, 소광률 및 빔품질(M2)은 각각 0.6%, 16.7 dB, M2x=1.21, M2y=1.22로 측정되었다.