• 대한의생명과학회지
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• 제23권4호
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• pp.416-420
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• 2017

Endoplasmic reticulum (ER) stress induces unfolded protein response (UPR) via inositol-requiring enzyme 1 (IRE1) activation, which sends a molecular signal for X box-binding protein 1 (XBP1) mRNA splicing in the cytosol. IRE1 endoribonuclease activity induces cleavage of XBP1 mRNA. The XBP1 mRNA is then ligated by an uncharacterized RNA ligase and translated to produce spliced XBP1 by 23 nt removed in which contains the PstI restriction enzyme site. The splicing of XBP1 mRNA can be detected by semiquantitative RT-PCR, and then splicing of XBP1 is a useful tool to measure the genetic variability in ER stress. In this study, we have estimated IRE1-dependent splicing of XBP1 mRNA under conditions of various hypothermia. The results indicated that hypothermia regulated ER stress. This study demonstrated that hypothermia is closely related to ER stress and may be useful for early diagnosis of ER-associated disease.

• 한국항해항만학회지
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• 제32권9호
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• pp.723-727
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• 2008

Sensing performances of evanescent field absorption (EFA) hetero-core fiber sensor has been presented based on EFA by changing the length and the core diameter of the single mode fiber. Experimental results have demonstrated a good feature in their relationship between the length and the core diameter of the single mode fiber. The sensor consists of 2 fiber optics which have the same cladding diameter of $125{\mu}m$ However one fiber optic used is single mode and has varying core diameter ranging from 3.3 to $5.6{\mu}m$. The other fiber is multimode type and has a thicker fixed core diameter of $62.5{\mu}m$. The 2 fiber optics are thermally spliced together. Experiments conducted to measure the resonance wavelength were carried out over a range of refractive index, to find the optimum sensing length Experiments show that core diameter of the single mode fiber and sensing length offects the linearity and sensitivity.

• 유공압시스템학회논문집
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• 제7권2호
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• pp.1-6
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• 2010

A new type fiber optic sensing system has been developed as a commercially available standard using the technique of hetero-core spliced fiber optic sensor, for the purposes of monitoring large scaled structures, preserving natural environments and measuring physical phenomenons. The sensing system has been tested and evaluated in a possible outdoor condition in view of the full scaled operation at actual sites to be monitored. Additionally, the developed system in this work conveniently provides us with various options of sensor modules intended to measure such physical quantities as displacement, distortion, pressure, binary states and liquid adhesion. The experiment study has been performed to examine the performance to a pseudo-cracking experiment in the outdoor situation, and to clarify temperature influences to the system in terms of the coupling of optical connectors and the OTDR stability. It has been verified that the sensing system is robust to the temperature change ranging from the general condition to the hard condition. Especially, in this study, the specification and performances of the pressure sensor have been demonstrated to show the capability of inspecting various physical quantities.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2001년도 추계 학술발표회 논문집 Proceedings of EESK Conference-Fall 2001
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• pp.194-199
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• 2001

Lap splice in plastic hinge region is inevitable because of due to constructional joint between footing and column. R/C Circular columns with lap-splice in plastic hinge region are widely used in Korean highway bridges. In addition, these columns which constructed before the seismic design code have a number of structural deficiencies. It is, however, believed that there are not many experimental research works for nonlinear behavior of these columns subjected to earthquake motions. The object of this research is to evaluate the seismic performance of existing circular reinforced concrete bridge piers by the Quasi-static test. Existing reinforced concrete bridge piers were moderate seismically designed in accordance with the conventional provisions of Korea Highway Design Specification. This study has been performed to verify the effect of lap spliced longitudinal steel, confinement steel type and confinement steel ratio far the seismic behavior of reinforced concrete bridge piers. Quasi-static test has been done to investigate the physical seismic performance of RC bridge piers, such as displacement ductility, energy absorption, strength degradation etc.

• Toxicological Research
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• 제24권2호
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• pp.129-135
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• 2008

The results of recent studies indicate that high levels of free fatty acids(FFAs) and adipokines may be the main causes of non-alcoholic liver disease; however, the molecular mechanism that links FFAs to lipotoxicity remains unclear. In the present study, we treated HepG2 cells with FFA(either palmitate or oleate) to investigate the mechanisms involved in lipotoxicity in the liver cells. We also treated cells with palmitate in the presence of a chemical chaperone, 4-phenylbutyric acid(PBA), to confirm the involvement of ER stress in lipotoxicity. Palmitate significantly induced cytotoxicity in dose- and time-dependent manners. Apoptosis was also significantly induced by palmitate as measured by caspase-3 activity and DAPI staining. Palmitate led to increased expressions of the spliced form of X-box-protein(Xbp)-1 mRNA and C/EBP homologous transcription factor(CHOP) protein, suggesting activation of the unfolded-protein response. PBA co-incubation significantly attenuated apoptosis induced by palmitate. The above data demonstrate that high levels of palmitate induce apoptosis via the mediation of ER stress in the liver cells and that chemical chaperones act to modulate ER stress and accompanying apoptosis.

• Genomics & Informatics
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• 제6권2호
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• pp.84-86
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• 2008

The Tetrahymena group I intron has been shown to employ a trans-splicing reaction and has been modified to specifically target and replace human telomerase reverse transcriptase (hTERT) RNA with a suicide gene transcript, resulting in the induction of selective cytotoxicity in cancer cells that express the target RNA, in animal models as well as in cell cultures. In this study, we evaluated the target RNA specificity of trans-splicing phenomena by the group I intron in mice that were intraperitoneally inoculated with hTERT-expressing human cancer cells to validate the anti-cancer therapeutic applicability of the group I intron. To this end, an adenoviral vector that encoded for the hTERT-targeting group I intron was constructed and systemically injected into the animal. 5'-end RACE-PCR and sequencing analyses of the trans-spliced cDNA clones revealed that all of the analyzed products in the tumor tissue of the virus-infected mice resulted from reactions that were generated only with the targeted hTERT RNA. This study implies the in vivo target specificity of the trans-splicing group I intron and hence suggests that RNA replacement via a trans-splicing reaction by the group I intron is a potent anti-cancer genetic approach.

• 대한의생명과학회지
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• 제11권2호
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• pp.249-252
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• 2005

The mammalian unfolded protein response (UPR) protects the cell. against the stress of unfolded or misfolded proteins in the endoplasmic reticulum (ER). It has recently demonstrated that IRE1, PERK, ATF6, and X-box protein 1 (XBP-l) directly or indirectly participate in this process. Upon accumulation of unfolded/misfolded proteins in the ER lumen, release of BiP from Ire1p permits dimerization and autophosphorylation to activate its kinase and endoribonulease activities to initiate XBP-1 mRNA splicing. Spliced XBP-1 mRNA removed middle part of 23 bp and encodes a potent transcription factor, XBP-l protein that binds to the unfolded protein response element (UPRE) or endoplasmic reticulum stress element (ERSE) sequence of many UPR target genes and produces several kind of ER chaperones. In this study, we described both the result and the detailed experimental procedures of XBP-1 mRNA splicing induced by ER stress, this result might help to elucidate the roles of the UPR and early diagnosis in a number of human diseases involving endoplasmic reticulum storage disease (ERSD).

• Molecules and Cells
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• 제25권4호
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• pp.504-509
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• 2008

Three G-protein-linked acetylcholine receptors (GARs) exist in the nematode C. elegans. GAR-3 is pharmacologically most similar to mammalian muscarinic acetylcholine receptors (mAChRs). We observed that carbachol stimulated ERK1/2 activation in Chinese hamster ovary (CHO) cells stably expressing GAR-3b, the predominant alternatively spliced isoform of GAR-3. This effect was substantially reduced by the phospholipase C (PLC) inhibitor U73122 and the protein kinase C (PKC) inhibitor GF109203X, implying that PLC and PKC are involved in this process. On the other hand, GAR-3b-mediated ERK1/2 activation was inhibited by treatment with forskolin, an adenylate cyclase (AC) activator. This inhibitory effect was blocked by H89, an inhibitor of cAMP-dependent protein kinase A (PKA). These results suggest that GAR-3b-mediated ERK1/2 activation is negatively regulated by cAMP through PKA. Together our data show that GAR-3b mediates ERK1/2 activation in CHO cells and that GAR-3b can couple to both stimulatory and inhibitory pathways to modulate ERK1/2.

• 한국구조물진단유지관리공학회 논문집
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• 제11권6호
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• pp.103-112
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• 2007

본 연구의 목적은 건설현장에서 노무비와 공사비를 절감하기 위하여 5개의 프리캐스트 세그먼트로 구성된 프리스트레스트 콘크리트 거더를 제작하여 그 역학적인 거동 특성을 평가하는데 있다. 본 연구를 위하여 총 4개의 25m 실험체를 동일 단면으로 제작하였으며, 모멘트-처짐 곡선으로 텐던변화와 접합부에 대한 분석과 해석을 수행하였다. 또한, 실험결과를 검증하기 위하여 2차원 비선형유한요소해석을 수행하였으며, 해석결과는 실험체의 모멘트-처짐곡선을 비교적 잘 예측하였다.

• 한국광학회지
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• 제12권4호
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• pp.289-293
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• 2001

금속 모세관을 이용하여 일반적인 단일모드 광섬유를 결합함으로써 매크로 벤딩(macro bending)을 측정할 수 있는 광섬유 센서를 제작하였다. 매크로 벤딩에 의한 곡률 반지름이 감소함에 따라 각도 정렬 오차가 증가하여 투과되는 빛의 에너지가 감소하게 되므로 매크로 벤딩을 측정할 수 있었다. 측정 범위는 곡률 반지름 20mm에서 85mm의 영역이었다. 사용한 광섬유 격자의 중심 파장은 각각 1543.3 nm, 1549.5nm이고 최대 벤딩 손실을 -11.19dB였다. 이 센서와 광섬유 브래그 격자를 이용하여 다중점 측정이 가능함을 보였다.