• 한국수정란이식학회지
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• 제30권3호
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• pp.143-147
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• 2015

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen Post-thawed in boar. Recently, polymorphism (g. 35756 T>C) of Estrogen Receptor 1 (ESR1) gene reported to be significant association with MOT. This study was conducted to evaluate the ESR1 gene as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results, The g.35756 T>C SNP of ESR1 was significantly associated with frozen semen motility and kinematic characteristics. The g.35756 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP (p<0.001). The SNP was also significantly associated with ALH (P<0.05). Therefore, we suggest that the g. 35756 T>C polymorphism in the intron 1 region of the porcine ESR1 gene could potentially be applied in frozen semen programs to improve MOT trait, but only after validation in other populations.

• 한국발생생물학회지:발생과생식
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• 제2권2호
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• pp.213-221
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• 1998

세포질내 정자주입술은 불임치료에 도입된 이후 남성불임 극복에 성공적으로 이용되고 있다. 세포질내 정자주입술 시행 후 수정률, 난할률과 발생된 배아의 상태는 여러 가지 요인에 의해 영향을 받으며, 난자의 상태에 따라 영향을 받는 지에 대해서 아직까지는 논란이 많다. 본 연구에서는 세포질내 정자주입술의 결과가 난자의 상태에 따라 영향을 받는지를 알아보기 위하여 세포질내 정자주입술을 시행한 44례에서 전과정을 현미경에 부착된 CCD 카메라를 통하여 녹화하였다. 그리고 각각의 난자에 대하여 세포질내 함유물의 존재 여부, 위란강의 넓이, 위란강내 세포질 잔기의 존재 여부, 난자의 세포막의 탄력성을 관찰, 기록하여 ICSI시행 후 수정률과 발생된 배아의 상태를 비교, 관찰하였다. 결과를 살펴보면 수정률은 세포질내 함유물의 존재 여부, 위란강의 넓이, 위란강내 세포질 잔기의 존재 여부에 따라서 유의한 차이를 나타내지 않았다. 난자의 세포막의 탄력성의 차이에 따른 퇴화율은 주입미세관이 들어가면서 난자의 세포막이 뚫리는 경우가 17.7%, 난자의 세포질을 흡입하여 난자의 세포막이 뚫린 경우가 1.6%로 유의한 차이 (P<0.001)를 나타내었다. 발생된 배아의 상태는 각각의 경우에 대해서 유의한 차이를 나타내지 않았다. 원인별 난자의 상태는, 여성요인 환자와 남성요인 환자에서 세포질내 함유물을 갖는 난자의 비율은 각각 48.4%, 25.1% (P<0.001), 위란강이 넓은 난자의 비율은 각각 35.2%, 19.0% (P<0.001), 위란강내 세포질 잔기가 존재하는 난자의 비율은 각각 53.3%, 38.4% (P<0.05)로 여성요인 환자에서 유의하게 높았다. 결론적으로 세포질내 정자주입술 후 수정률과 발생된 배아의 발생률은 광학현미경하에서 관찰한 난자의 상태와 상관관계가 없는 것으로 사료되며, 세포질내 정자주입술 후 난자의 퇴화율은 난자 세포막의 탄력성과 상관관계가 있는 것으로 생각된다.rine 또는 autocrine조절물질로 작용하여 GH 분비 외에도 뇌하수체 전엽 세포들의 분화와 분열등에 관여함을 시사한다..3 unit의 잔존활성을 보였으나 중심온도가 9$0^{\circ}C$와 10$0^{\circ}C$일 때 잔존활성은 측정되지 않았다.>에서 뚜렷한 효과를 나타내었다.비율이 높은 반면 Malaysia 거주자들은 중국음식, 만두 및 면류의 이용이 비교적 많은 편이었다. 이상의 결과를 종합해 보면 외국에 거주하면서 한국의 전통적인 특성을 간직하고자 하는 의식을 가지고 있음을 볼 수 있으며 하루 세끼의 식사형태에서 밥을 중심으로 하는 비율은 국내 보다 크게 낮았지만 여전히 다른 식사형태 보다 높은 것을 알 수 있다. 한국 고유의 식문화 특성을 가지면서 거주지역의 식품생산 및 사회 문화적 특성에 영향을 받음으로써 거주국의 식품, 식사 형태, 조리방법을 도입하는 등 부분적으로 변화가 진행되고 있음을 확인 할 수 있었다.가장 시급한 개선점이라고 생각된다. 따라서 효과적인 급식운영을 하기 위해서는 학교식당은 맛, 다양한 메뉴의 식단, 위생, 가격의 저렴 등을 통해서 급식대상자들이 만족한 급식을 할 수 있도록 체계적인 관리 및 운영을 통해 적극적으로 개선해 나가야 할 것으로 사료된다.er (p<0.05), $T_{max}$ might be an insignificant parameter because cyclosporin A generally requires long-term administration. From these results, the two products are bioequivalent.alent.t.>, C-a. and T-between the two products (7.18%, 9.58% and 7.53%, respectively) were less than 20%. The power (1-7) and

• Asian-Australasian Journal of Animal Sciences
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• 제11권1호
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• pp.55-59
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• 1998

To study the effect of induced hypothyroidism on fertility status of male Black Bengal goats, 10 adult healthy mature males were divided into control and treated groups. Hypothyroidism was induced successfully by injecting thiourea subcutaneously initially for 15 days at the rate of 100 mg/kg body weight, followed by 66.7 mg/kg body weight for the subse quent 15 days. This resulted in a decrease (p < 0.01) in plasma tri-iodothyronine, thyroxine and testosterone levels, with increasing duration of thiourea treatment. It also adversely affected semen quality, ejaculate volume, sperm concentration, motility and viability. Live percentage declined to 75% for treated and control remained at 90%. Artificial insemination fo female goats with semen of thiourea-treated goats, resulted in failure of conception. However, females inseminated with semen of male goats of control group showed 100% conception. These observations indicate that, thyroid hormones play a key role in maintaining the normal reproductive processes of male goats.

• 한국식품영양과학회지
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• 제28권5호
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• pp.1180-1186
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• 1999

A wide ranges of chemicals released into the environment have potential to interfere with physiological and development process by disrupting endocrine pathways. Endocrine system embraces a multitude of mechanisms of action, including effect on growth, behavior, reproduction and immune function. These environmental endocrine disruptors are present in environment and pose potential health consequences to human and wildlife. The best known form in endocrine distruptors involves substances which mimic or block the action of natural hormone in the body. Endocrine disruptor have been variously defined as exogenous agents that interfere with the synthesis, secretion, transport, metabolism, binding action or elimination of the natural hormones in the body which are responsible for the maintenance of homeostasis, reproduction developmental and/or behavior. Many compounds polluted into the environment by human activity are capable of disrupting the endocrine system of animals, including fish, wildlife, and humans. Among these chemicals are pesticides, industrial chemicals, and other anthropogenic products. It has been alleged that several adverse effects on human health are linked with exposure to chemicals which are claimed to be endocrine disrupters, that is, increased incidence of testicular, prostate and female breast cancer, time dependent reductions in sperm quality and quantity, increased incidence of cryptorchidism (undescended testicles) and hypospadias(malformation of the penis), altered physical and mental de velopment in children. This observation is currently the only example of chemically mediated endocrine disruption which has resulted in a clear effect at the population level.

• 대한생식의학회:학술대회논문집
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• 대한생식의학회 2007년도 제52차 춘계학술대회 초록집
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• pp.147-147
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• 2007

• 운동과학
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• 제26권3호
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• pp.204-211
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• 2017

PURPOSE: This study was to investigate the effects of resistance exercise on serum inflammatory markers and CatSper 1-4 expression in testis of OLETF rats. METHODS: Male OLETF rats were divided into two groups; control group (n=12), resistance exercise group (n=12). The exercise group performed a total of 8 weeks of moderate intensity resistance exercise on a 1.35 m vertical ladder with weights secured to their tails. RESULTS: The results of this study was following; The exercise group showed a significant decreased in the inflammatory ($IL-1{\beta}$, IL-6, $TNF-{\alpha}$) levels as compared to the control group. But CRP was no significant difference between control and exercise groups. Also, CatSper 1 and 2 were significantly increased in the exercise group compared to the control group, whereas CatSper 3, 4 were no significant difference between both groups. CONCLUSIONS: This study suggests that resistance exercise training can contribute to reduce pro-inflammatory responses in whole body and it affects male reproductive function by improve sperm quality and CatSper protein expression.

• Clinical and Experimental Reproductive Medicine
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• 제45권4호
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• pp.189-194
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• 2018

Objective: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. Methods: Immature oocytes were obtained by needle aspiration from 49 pregnant women (group 1) who underwent a cesarean section at term and 77 non-pregnant women (group 2) who underwent a gynecological operation during the same period (8 months). Healthy immature oocytes (530 in group 1 and 539 in group 2) were cultured and assessed for maturation 36 hours later. Mature oocytes were inseminated by intracytoplasmic sperm injection and cultured up to 144 hours. Results: The percentage of degenerated oocytes was significantly higher (12.1% vs. 6.3%; p<0.001) in group 1 than in group 2. There was no significant difference in the maturation rate (66.8% vs. 68.1%; p=0.698), fertilization rate (66.7% vs. 67.6%; p=0.857), or the rate of formation of good-quality blastocysts (46.2% vs. 47.2%; p=0.898) in oocytes obtained from pregnant and non-pregnant women. Conclusion: The developmental competence of immature oocytes did not differ between pregnant and non-pregnant women.

• Clinical and Experimental Reproductive Medicine
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• 제49권1호
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• pp.70-73
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• 2022

Objective: This study was conducted to assess the efficacy of subcutaneous granulocyte colony-stimulating factor (G-CSF) for treating thin endometrium. Methods: Data from 88 infertile women with thin endometrium (<7 mm) aged 23 to 40 years were evaluated retrospectively over a period of 1 year. In group 1, subcutaneous infusion of G-CSF (300 ㎍/mL) was administered to 44 women along with other supplemental treatments. If the lining did not exceed 7 mm within 72 hours, a second infusion was administered. In group 2, which also had 44 women, only estradiol valerate and sildenafil were administered, while subcutaneous G-CSF infusion was not. Embryo transfers were performed once the lining exceeded 7.5 mm. The efficacy of G-CSF was evaluated by assessing the thickness of the endometrium before embryo transfer, pregnancy rates, and clinical pregnancy rates. Results: There were no differences between the groups regarding demographic variables, egg reserves, sperm parameters, the number of embryos transferred, and embryo quality. The pregnancy rate was significantly higher in group 1 (60%, 24 of 40 cases) than in group 2 (31%, 9 of 29 cases) (p<0.001). The clinical pregnancy rate was also significantly higher in group 1 (55%) than in group 2 (24%) (p<0.001). Conclusion: Subcutaneous G-CSF infusion improved the thickness of the endometrium when it was thin. To the best of our knowledge, this is the first documented study to clearly demonstrate the benefits of subcutaneous G-CSF infusion for treating thin endometrium.

• Clinical and Experimental Reproductive Medicine
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• 제48권4호
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• pp.362-367
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• 2021

Objective: The impact of early mechanical removal of cumulus cells on fertilization and embryonic development is not yet precisely known. This study aimed to investigate the effects of early and late cumulus cell removal on fertilization, polyspermy, embryonic development potential, blastocyst development, and clinical outcomes. Methods: A prospective study was conducted of patients who underwent in vitro fertilization between September 2019 and October 2020. Sibling oocytes were randomly allocated after insemination to early cumulus cell removal at 6 hours (group I) and late cumulus cell removal at 16-18 hours (group II). If total fertilization failure (TFF) was determined to have occurred at early cumulus cell removal, rescue intracytoplasmic sperm injection (ICSI) was performed. Fertilization, embryonic development, and pregnancy outcomes were compared. Results: A total of 912 oocytes were assigned to group I (458 oocytes) and group II (454 oocytes). Fertilization, polyspermy, embryo quality, and pregnancy outcomes were not significantly different between both groups. Rescue ICSI enabled fertilization of 79.2% of the TFF oocytes. Conclusion: Early cumulus cell removal at 6 hours had no significant difference in fertilization, polyspermy, embryo development, or obstetric and perinatal outcomes compared to late removal. Early cumulus cell removal combined with early rescue ICSI may have the potential to help couples with TFF.

• Asian-Australasian Journal of Animal Sciences
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• 제31권8호
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• pp.1119-1126
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• 2018

Objective: Present investigation was aimed to study the Single Nucleotide Variants of the luteinizing hormone beta ($LH{\beta}$) gene and to analyze their association with the semen quality (fresh and post-thawed frozen semen) and luteinizing hormone (LH) concentrations in Murrah buffalo bulls. Methods: Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and Sanger sequencing method is used to study genetic variability in $LH{\beta}$ gene. LH assay was carried out using enzyme-linked immunosorbent assay method. A fixed general linear model was used to analyze association of single nucleotide polymorphism (SNP) of $LH{\beta}$ gene with semen quality in 109 and LH concentrations in 80 Murrah bulls. Results: $LH{\beta}$ gene was found to be polymorphic. Total six SNPs were identified in $LH{\beta}$ gene g C356090A, g C356113T, g A356701G, g G355869A, g G356330C, and g G356606T. Single Stranded Conformational Polymorphism variants of pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 had highly significant (p<0.01) effect on sperm concentration (million/mL), percent mass motility, acrosome integrity and membrane integrity in fresh and frozen semen whereas significant (p<0.05) effect was observed on percent live spermatozoa. SSCP variants of pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 had highly significant (p<0.01) effect on luteinizing hormone concentrations too. Conclusion: The observed association between SSCP variants of $LH{\beta}$ gene with semen quality parameters and LH concentrations indicated the possibilities of using $LH{\beta}$ as a candidate gene for identification of markers for semen quality traits and LH concentrations in Murrah buffaloes.