Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
권호 표지
DOI QR코드

DOI QR Code

Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women

  • Pongsuthirak, Pallop (Department of Obstetrics and Gynecology, Buddhachinaraj Hospital Medical School) ;
  • Vutyavanich, Teraporn (Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University)
  • Received : 2018.06.27
  • Accepted : 2018.09.07
  • Published : 2018.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. Methods: Immature oocytes were obtained by needle aspiration from 49 pregnant women (group 1) who underwent a cesarean section at term and 77 non-pregnant women (group 2) who underwent a gynecological operation during the same period (8 months). Healthy immature oocytes (530 in group 1 and 539 in group 2) were cultured and assessed for maturation 36 hours later. Mature oocytes were inseminated by intracytoplasmic sperm injection and cultured up to 144 hours. Results: The percentage of degenerated oocytes was significantly higher (12.1% vs. 6.3%; p<0.001) in group 1 than in group 2. There was no significant difference in the maturation rate (66.8% vs. 68.1%; p=0.698), fertilization rate (66.7% vs. 67.6%; p=0.857), or the rate of formation of good-quality blastocysts (46.2% vs. 47.2%; p=0.898) in oocytes obtained from pregnant and non-pregnant women. Conclusion: The developmental competence of immature oocytes did not differ between pregnant and non-pregnant women.

Keywords

References

  1. Pongsuthirak P, Vutyavanich T. Developmental competence of human embryos derived from in vitro maturation of immature oocytes retrieved during cesarean section. J Obstet Gynaecol Res 2014;40:459-64. https://doi.org/10.1111/jog.12186
  2. Pongsuthirak P, Songveeratham S, Vutyavanich T. Comparison of blastocyst and Sage media for in vitro maturation of human immature oocytes. Reprod Sci 2015;22:343-6. https://doi.org/10.1177/1933719114542027
  3. Behboodi E, Anderson GB, BonDurant RH. Development of in vitro fertilized oocytes from pregnant and nonpregnant cows in oviductal epithelial and cumulus cell co-culture systems. Theriogenology 1992;38:1077-84. https://doi.org/10.1016/0093-691X(92)90121-7
  4. Gambini A, Jarazo J, Karlanian F, De Stefano A, Salamone DF. Effect of collection-maturation interval time and pregnancy status of donor mares on oocyte developmental competence in horse cloning. J Anim Sci 2014;92:561-7. https://doi.org/10.2527/jas.2013-7026
  5. Torner H, Heleil B, Alm H, Ghoneim IM, Srsen V, Kanitz W, et al. Changes in cumulus-oocyte complexes of pregnant and nonpregnant camels (Camelus dromedarius) during maturation in vitro. Theriogenology 2003;60:977-87. https://doi.org/10.1016/S0093-691X(03)00099-2
  6. Brzyski RG, Leland MM, Eddy CA. In vitro maturation of baboon oocytes retrieved at the time of cesarean section. Fertil Steril 1999;71:1153-6. https://doi.org/10.1016/S0015-0282(99)00130-2
  7. Xu M, Fazleabas AT, Shikanov A, Jackson E, Barrett SL, Hirshfeld- Cytron J, et al. In vitro oocyte maturation and preantral follicle culture from the luteal-phase baboon ovary produce mature oocytes. Biol Reprod 2011;84:689-97. https://doi.org/10.1095/biolreprod.110.088674
  8. Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Yoon TK. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril 1991;55:109-13. https://doi.org/10.1016/S0015-0282(16)54068-0
  9. Trounson A, Wood C, Kausche A. In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril 1994;62:353-62. https://doi.org/10.1016/S0015-0282(16)56891-5
  10. Barnes FL, Crombie A, Gardner DK, Kausche A, Lacham-Kaplan O, Suikkari AM, et al. Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod 1995;10:3243-7. https://doi.org/10.1093/oxfordjournals.humrep.a135896
  11. Chian RC, Buckett WM, Tulandi T, Tan SL. Prospective randomized study of human chorionic gonadotrophin priming before immature oocyte retrieval from unstimulated women with polycystic ovarian syndrome. Hum Reprod 2000;15:165-70. https://doi.org/10.1093/humrep/15.1.165
  12. Lin YH, Hwang JL, Huang LW, Mu SC, Seow KM, Chung J, et al. Combination of FSH priming and hCG priming for in-vitro maturation of human oocytes. Hum Reprod 2003;18:1632-6. https://doi.org/10.1093/humrep/deg335
  13. Hwang JL, Lin YH, Tsai YL. In vitro maturation and fertilization of immature oocytes: a comparative study of fertilization techniques. J Assist Reprod Genet 2000;17:39-43. https://doi.org/10.1023/A:1009450014064
  14. Hwu YM, Lee RK, Chen CP, Su JT, Chen YW, Lin SP. Development of hatching blastocysts from immature human oocytes following in-vitro maturation and fertilization using a co-culture system. Hum Reprod 1998;13:1916-21. https://doi.org/10.1093/humrep/13.7.1916
  15. Hwang JL, Lin YH, Tsai YL. Pregnancy after immature oocyte donation and intracytoplasmic sperm injection. Fertil Steril 1997;68:1139-40. https://doi.org/10.1016/S0015-0282(97)00398-1
  16. Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod 2011;26:1270-83. https://doi.org/10.1093/humrep/der037
  17. Baerwald AR, Adams GP, Pierson RA. Ovarian antral folliculogenesis during the human menstrual cycle: a review. Hum Reprod Update 2012;18:73-91. https://doi.org/10.1093/humupd/dmr039
  18. Teramoto S, Osada H, Sato Y, Shozu M. Nondominant small follicles are a promising source of mature oocytes in modified natural cycle in vitro fertilization and embryo transfer. Fertil Steril 2016;106:113-8. https://doi.org/10.1016/j.fertnstert.2016.03.015
  19. Demirtas E, Elizur SE, Holzer H, Gidoni Y, Son WY, Chian RC, et al. Immature oocyte retrieval in the luteal phase to preserve fertility in cancer patients. Reprod Biomed Online 2008;17:520-3. https://doi.org/10.1016/S1472-6483(10)60239-8
  20. Maman E, Meirow D, Brengauz M, Raanani H, Dor J, Hourvitz A. Luteal phase oocyte retrieval and in vitro maturation is an optional procedure for urgent fertility preservation. Fertil Steril 2011;95:64-7. https://doi.org/10.1016/j.fertnstert.2010.06.064
  21. Oktay K, Demirtas E, Son WY, Lostritto K, Chian RC, Tan SL. In vitro maturation of germinal vesicle oocytes recovered after premature luteinizing hormone surge: description of a novel approach to fertility preservation. Fertil Steril 2008;89:228.e19-22.
  22. Moffat R, Pirtea P, Gayet V, Wolf JP, Chapron C, de Ziegler D. Dual ovarian stimulation is a new viable option for enhancing the oocyte yield when the time for assisted reproductive technnology is limited. Reprod Biomed Online 2014;29:659-61. https://doi.org/10.1016/j.rbmo.2014.08.010
  23. Ubaldi FM, Capalbo A, Vaiarelli A, Cimadomo D, Colamaria S, Alviggi C, et al. Follicular versus luteal phase ovarian stimulation during the same menstrual cycle (DuoStim) in a reduced ovarian reserve population results in a similar euploid blastocyst formation rate: new insight in ovarian reserve exploitation. Fertil Steril 2016;105:1488-95.e1. https://doi.org/10.1016/j.fertnstert.2016.03.002
  24. Govan AD. The human ovary in early pregnancy. J Endocrinol 1968;40:421-8. https://doi.org/10.1677/joe.0.0400421
  25. Govan AD. Ovarian follicular activity in late pregnancy. J Endocrinol 1970;48:235-41. https://doi.org/10.1677/joe.0.0480235
  26. Westergaard L, McNatty KP, Christensen IJ. Steroid concentrations in fluid from human ovarian antral follicles during pregnancy. J Endocrinol 1985;107:133-6. https://doi.org/10.1677/joe.0.1070133
  27. Cha KY, Do BR, Chi HJ, Yoon TK, Choi DH, Koo JJ, et al. Viability of human follicular oocytes collected from unstimulated ovaries and matured and fertilized in vitro. Reprod Fertil Dev 1992;4:695-701. https://doi.org/10.1071/RD9920695
  28. Kim M, Hong SJ, Lee JH, Min CK, Hwang KJ, Park RW. Comparison of in vitro maturation media of immature oocytes: the effectiveness of blastocyst culture media. Fertil Steril 2011;95:554-7. https://doi.org/10.1016/j.fertnstert.2010.10.035
  29. Grynberg M, Poulain M, le Parco S, Sifer C, Fanchin R, Frydman N. Similar in vitro maturation rates of oocytes retrieved during the follicular or luteal phase offer flexible options for urgent fertility preservation in breast cancer patients. Hum Reprod 2016;31:623-9. https://doi.org/10.1093/humrep/dev325

Cited by

  1. The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitro vol.19, pp.1, 2021, https://doi.org/10.1186/s12958-021-00771-8