Clinical and Experimental Reproductive Medicine

  • 제45권4호
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  • Pages.189-194
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  • 2018
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Developmental competence of in vitro-matured human oocytes obtained from pregnant and non-pregnant women

  • Pongsuthirak, Pallop (Department of Obstetrics and Gynecology, Buddhachinaraj Hospital Medical School) ;
  • Vutyavanich, Teraporn (Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University)
  • 투고 : 2018.06.27
  • 심사 : 2018.09.07
  • 발행 : 2018.12.31
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초록

Objective: The aim of this study was to compare the rate of maturation, fertilization, and embryo development of in vitro-matured human oocytes derived from pregnant and non-pregnant women. Methods: Immature oocytes were obtained by needle aspiration from 49 pregnant women (group 1) who underwent a cesarean section at term and 77 non-pregnant women (group 2) who underwent a gynecological operation during the same period (8 months). Healthy immature oocytes (530 in group 1 and 539 in group 2) were cultured and assessed for maturation 36 hours later. Mature oocytes were inseminated by intracytoplasmic sperm injection and cultured up to 144 hours. Results: The percentage of degenerated oocytes was significantly higher (12.1% vs. 6.3%; p<0.001) in group 1 than in group 2. There was no significant difference in the maturation rate (66.8% vs. 68.1%; p=0.698), fertilization rate (66.7% vs. 67.6%; p=0.857), or the rate of formation of good-quality blastocysts (46.2% vs. 47.2%; p=0.898) in oocytes obtained from pregnant and non-pregnant women. Conclusion: The developmental competence of immature oocytes did not differ between pregnant and non-pregnant women.

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참고문헌

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피인용 문헌

  1. The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitro vol.19, pp.1, 2021, https://doi.org/10.1186/s12958-021-00771-8