• Mass Spectrometry Letters
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• v.5 no.2
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• pp.42-48
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• 2014

A specific and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry method (LC-ESI-MS/MS) was developed and validated for the simultaneous quantification of porphyrins (coproporphyrin, pentacarboxylporphyrin, hexacarboxylporphyrin, heptacarboxylporphyrin, and uroporphyrin) in human plasma and urine. Acidified plasma samples and urine samples were prepared by using liquid-liquid extraction using ethyl acetate and protein precipitation with acetonitrile, respectively. The separation was achieved onto a Synergi Fusion RP column ($150mm{\times}2.0mm$, $4{\mu}m$) with a gradient elution of mobile phase A (0.1% formic acid in 2 mmol/L ammonium acetate, v/v) and mobile phase B (20% methanol in acetonitrile, v/v) at a flow rate of $450{\mu}L$/min. Porphyrins and the internal standard (IS), coproporphyrin I-$^{15}N_4$, were detected by a tandem mass spectrometer equipped with an electrospray ion source operating in positive ion mode. Multiple reaction monitoring (MRM) transitions of the protonated precursor ions and the related product ions were optimized to increase selectivity and sensitivity. The proposed method was validated by assessing selectivity, linearity, limit of quantification (LOQ), precision, accuracy, recovery, and stability. The calibration curves were obtained in the range of 0.1-100 nmol/L and the LOQs were estimated as 0.1 nmol/L for all porphyrins. Results obtained from the validation study of porphyrins showed good accuracy, precision, recovery, and stability. Finally, the proposed method was successfully applied to clinical studies on the autism spectrum disorder (ASD) diagnosis of 203 Korean children.

• Korean Journal of Environment and Ecology
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• v.23 no.2
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• pp.105-115
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• 2009

The naturalized plants in the Byeonsanbando National Park were listed 88 taxa composed of 24 families, 65 genus, 83 species and 3 varieties and 2 forms by field survey. The naturalized plants divided into pattern by survey of annual plants ratio is 38 taxa (43.2%) by life form spectrum and perennials is 28 taxa (31.8%), biennials is 19 taxa (21.6%), two trees (Robinia pseudoacacia, Ailanthus altissima) and one shrub (Amorpha furticosa) were founded. According to analysis results form place of origin, Europe covered 26 taxa (37.1%), North America covered 23 taxa (32.8%). Naturalized degree 3 plants, as common but not abundant, founded as 28 taxa (31.8%) were hold most highly ratio, naturalized degree 1 plants founded as 8 taxa (9.1%) were hold lowest. Introduction period 1 covered 39 taxa (44.3%) have had highest score and period 2 covered 16 taxa (18.2%) have had lowest scores. In addition, the urbanization index based on 271 taxa was 32.5% and Ambrosia artemisiaefolia is growing by ecosystem disturbing wild plants. Meanwhile, Ambrosia artemisiaefolia have confirmed into district of introduction in the Byeonsanbando National Park were damage the human body of plants develop an allergy to pollen. Therefore, management counterplan of the sequel of monitoring them might be required, introduction into naturalized plants are grasp of ecological characteristics and systematical management plan from this point of time by require.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.36 no.3
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• pp.349-359
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• 2016

In order to analytically evaluate buffeting responses, the analysis of wind characteristics such as turbulence intensity, turbulence length, gust, roughness coefficient, etc must be a priority. Static aerodynamic force coefficients, flutter coefficients, structural damping ratios, aerodynamic damping ratios and natural frequencies affect the analytical responses. The bridge interested in this paper has being been used for 32 years. As the time passes, current terrain conditions around the bridge are different markedly from the conditions it was built 32 years ago. Also, wind environments were considerably varied by the climate change. For this reason, it is necessary to evaluate the turbulence intensity, length, spectrum and roughness coefficient of the bridge site from full-scale measurements using the structural health monitoring system. The evaluation results indicate that wind characteristics of bridge site is analogous to that of open terrain although the bridge is located on the coastal area. To calculate buffeting responses, the analysis variables such as damping ratios, static aerodynamic force coefficients and natural frequency were evaluated from measured data. The analysis was performed with regard to 4 cases. The evaluated variables from measured data are applied to the first and second analysis cases. And the other analysis cases were performed based on Design Guidelines for Steel Cable Supported Bridges. The calculated responses of each analysis cases are compared with the buffeting response measured at less than 25m/s wind speed. It is verified that the responses by the numerical analysis applying the estimated variables based on full-scale measurements are well agreed with the measured actual buffeting responses under wind speed 25m/s. Also, the extreme wind speed corresponding to a recurrence interval 200 years is derived from Gumbel distribution. The derived wind speed for return period of 200 years is 45m/s. Therefore the buffeting responses at wind speed 45m/s is determined by the analysis applying the estimated variables.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.43 no.10
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• pp.928-935
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• 2015

This paper presents low temperature structural tests of a UAV wing which has room temperature-curing adhesive bond. The wing structure is made of carbon fiber reinforced composites, and the skins are bonded to the inner structures (such as ribs and spars) using room temperature-curing adhesive bond. Also, to verify damage tolerance design of the wing structure, barely visible impact damages are intentionally created in the critical areas. The attachment fittings of the wing are fixed in a specially designed chamber which can simulate the low temperature environments of the operating altitudes. The test load is applied by hydraulic actuators which are placed outside the chamber. The structural tests consist of strain survey tests and a durability test for 1-life fatigue load spectrum. During the tests, strains of major parts are measured by strain gauges and FBG sensors. The change of the initial impact damages is also monitored using piezoelectric sensors. The 1-life damage tolerance of the composite structure is verified by the structural tests under the simulated environments.

• Korean Journal of Optics and Photonics
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• v.19 no.1
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• pp.68-72
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• 2008

Fiber-optic strain sensors based on Bragg reflection gratings produce the change of reflection spectrum when an external stress is applied on the sensor. To measure the Bragg reflection wavelength in high speed, an arrayed waveguide grating device is incorporated in this work. By monitoring the output power from each channel of the AWG, the peak wavelength corresponding to the applied strain could be obtained. To enhance the accuracy of the AWG wavelength interrogation system, a chirped fiber Bragg grating with a 3-dB bandwith of 5.4 nm is utilized. The high-speed response of the proposed system is demonstrated by measuring a fast varying strain produced by the damped oscillation of a cantilever. An oscillation frequency of 17.8 Hz and a damping time constant of 0.96 second are obtained in this measurement.

• Korean Journal of Optics and Photonics
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• v.22 no.2
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• pp.67-71
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• 2011

In the paper, we have demonstrated an azobenzene-coated fiber Bragg grating (FBG) for monitoring ultraviolet light (UV) intensity in remote measurement. The elasticity of the coated azobenzene polymer is changed by the UV light, which induces a center wavelength change corresponding to the change of the FBG's grating period. The wavelength shift resulting from both UV light and other light with the wavelength out of the UV range was about 0.18 nm. In order to improve the accuracy of the measurement, the center wavelength shift caused by radiant heat of the light source was sufficiently removed by using a thermal filter. The amount of the center wavelength shift was consequently reduced to 0.06 nm, compared to the result without the thermal filter. Also, the FBGs coated by using azobenzene polymer were produced by two different methods; thermal casting and UV curing. Considering temperature dependence, UV curing is more suitable than thermal casting in UV sensor application of the azobenzene-coated FBG. In addition, we have confirmed the wavelength dependence of the optical sensor by means of four different band pass filters. Thus, we found out that the center wavelength shift per unit intensity is 0.029 [arb. unit] as a maximum value at 370 nm wavelength region and that the absorption spectrum of the azobenzene polymer was very consistent with the wavelength dependence of the azobenzene-coated FBG.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.05a
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• pp.554-557
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• 2016

Recently, supercomputer has been increasingly adopted as a computing environment for scientific simulation as well as education, healthcare and national defence. Especially, supercomputing system with heterogeneous computing resources is gaining resurgence of interest as a next-generation problem solving environment, allowing theoretical and/or experimental research in various fields to be free of time and spatial limits. However, traditional supercomputing services have only been handled through a simple form of command-line based console, which leads to the critical limit of accessibility and usability of heterogeneous computing resources. To address this problem, in this paper, we provide the design and implementation of web-based HPC (High Performance Computing) job management framework for computational scientific simulation. The proposed framework has highly extensible design principles, providing the abstraction interfaces of job scheduler (as well as bundle scheduler plug-ins for LoadLeveler, Sun Grid Engine, OpenPBS scheduler) in order to easily incorporate the broad spectrum of heterogeneous computing resources such as cluster, computing cloud and grid. We also present the detailed specification of HTTP standard based RESTful endpoints, which manage simulation job's life-cycles such as job creation, submission, control and status monitoring, etc., enabling various 3rd-party applications to be newly created on top of the proposed framework.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.6 s.121
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• pp.629-638
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• 2007

The methods of bandwidth measurement in the field environments are notified in ITU-R spectrum monitoring(SM) documents. But, these methods for terrestrial DTV and T-DMB are not informed yet. The x-dB bandwidth is the most suitable method fer the bandwidth measurement of terrestrial digital broadcasting signals. So, we proposed the suitable x-dB value for x-dB bandwidth measurement of the digital broadcasting signal that is not notified in ITU-R SM documents. As a result, we derived the x dB value of -12 dB and -8 dB that can be used fer estimation of occupied bandwidth of DTV and T-DMB signals respectively by x dB bandwidth measurement in field environments.

• Journal of Korean Society of Health-System Pharmacists
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• v.35 no.4
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• pp.391-399
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• 2018

Background : The Antimicrobial Stewardship Program promotes interdisciplinary interventions and targeted recommendations for the proper utilization of antibiotics. In particular, the aim of the program is to avoid indiscriminate use of broad-spectrum antibiotics based on the documented literature on the significant impact of unsystematic usage of antibiotics on the distribution of antibiotic-resistant microorganisms. To improve the care process for pneumonia treatment using antimicrobial agents, institution-level guidelines were established and disseminated at the Seoul National University Bundang Hospital in April 2016. In this study, we evaluated changes in the physicians'antibiotic prescribing patterns both before-and after-the implementation of the guidelines. Methods : The electronic medical records of inpatients who were prescribed with one or more antibiotics in May 2014 (Group A) and May 2016 (Group B) were reviewed. Data on demographic characteristics, clinical outcomes, and antibiotic prescriptions were collected and the prescription records were compared both before- and after- the implementation of the guidelines. Results : A total of 180 patients were included in the study: 77 patients in group A and 103 patients in group B. The baseline characteristics of the patients were not significantly different between the two groups. Community-acquired pneumonia was the most common diagnosis in both the groups and the difference was not significant (68.8% vs. 67.9%; p=0.67). The type of antibiotic prescriptions used for empirical treatment was not different between the two groups. The most commonly prescribed empirical antibiotics were cephalosporins, with no significant difference (p=0.31). One of the most inappropriately used antibiotics was piperacillin/tazobactam and the rate of prescription was similar in both the groups (p=0.68). The rates of appropriate empirical selection of antibiotics remained unaltered between the two groups (67.5% vs. 71.8%; p=0.53). Conclusions : Implementation of the guidelines only exhibited no significant effect on the antibiotic prescribing patterns of physicians for the treatment of pneumonia. To improve the adequate use of empiric antibiotics, more active interventions and closer monitoring of the feedbacks should be additionally considered and evaluated in future studies.

• Pediatric Infection and Vaccine
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• v.31 no.1
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• pp.46-54
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• 2024

Purpose: This study aimed to identify the pathogens of bloodstream infection in children with underlying hemato-oncologic diseases, analyze susceptibility patterns, compare temporal trends with those of previous studies, and assess empirical antimicrobial therapy. Methods: Retrospective review study of children bacteremia in hemato-oncologic diseases was conducted at Seoul National University Bundang Hospital from January 2013 to July 2023. Results: Overall, 98 episodes of bacteremia were observed in 74 patients. Among pathogens isolated, 57.1% (n=56) were Gram-positive bacteria, 38.8% (n=38) were Gram-negative bacteria, and 4.1% (n=4) were Candida spp. The most common Gram-positive bacteria were coagulase-negative staphylococci (n=21, 21.4%) and Staphylococcus aureus, (n=14, 14.3%) whereas the most common Gram-negative bacteria were Klebsiella pneumoniae (n=16, 16.3%) and Escherichia coli (n=10, 10.2%). The susceptibility of Gram-positive bacteria to penicillin, oxacillin, and vancomycin was 11.5%, 32.7%, and 94.2%, respectively and the susceptibility of Gram-negative bacteria to cefotaxime, piperacillin/tazobactam, imipenem, gentamicin, and amikacin was 68.6%, 80%, 97.1%, 82.9%, and 91.4%, respectively. Methicillin-resistant S. aureus was detected in 1 strain and among Gram-negative strains, extended spectrum β-lactamase accounted for 28.9% (12/38). When analyzing the antibiotic susceptibility and empirical antibiotics, the mismatch rate was 25.5% (n=25). The mortality rate of children within 30 days of bacteremia was 7.1% (n=7). Conclusions: Empirical antibiotic therapy for bacteremia in children with hemato-oncologic diseases should be based on the local antibiogram in each institution and continuous monitoring is necessary.