• Title/Summary/Keyword: Spectral studies

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Effects of fermentation on protein profile of coffee by-products and its relationship with internal protein structure measured by vibrational spectroscopy

  • Samadi;Xin Feng;Luciana Prates;Siti Wajizah;Zulfahrizal;Agus Arip Munawar;Peiqiang Yu
    • Animal Bioscience
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    • v.36 no.8
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    • pp.1190-1198
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    • 2023
  • Objective: To our knowledge, there are few studies on the correlation between internal structure of fermented products and nutrient delivery from by-products from coffee processing in the ruminant system. The objective of this project was to use advanced mid-infrared vibrational spectroscopic technique (ATR-FT/IR) to reveal interactive correlation between protein internal structure and ruminant-relevant protein and energy metabolic profiles of by-products from coffee processing affected by added-microorganism fermentation duration. Methods: The by-products from coffee processing were fermented using commercial fermentation product, called Saus Burger Pakan, consisting of various microorganisms: cellulolytic, lactic acid, amylolytic, proteolytic, and xylanolytic microbes, for 0, 7, 14, 21, and 28 days. Protein chemical profiles, Cornell Net Carbohydrate and Protein System crude protein and CHO subfractions, and ruminal degradation and intestinal digestion of protein were evaluated. The attenuated total reflectance-Ft/IR (ATR-FTIR) spectroscopy was used to study protein structural features of spectra that were affected by added microorganism fermentation duration. The molecular spectral analyses were carried using OMNIC software. Molecular spectral analysis parameters in fermented and non-fermented by-products from coffee processing included: Amide I area (AIA), Amide II (AIIA) area, Amide I heigh (AIH), Amide II height (AIIH), α-helix height (αH), β-sheet height (βH), AIA to AIIA ratio, AIH to AIIH ratio, and αH to βH ratio. The relationship between protein structure spectral profiles of by-products from coffee processing and protein related metabolic features in ruminant were also investigated. Results: Fermentation decreased rumen degradable protein and increased rumen undegradable protein of by-products from coffee processing (p<0.05), indicating more protein entering from rumen to the small intestine for animal use. The fermentation duration significantly impacted (p<0.05) protein structure spectral features. Fermentation tended to increase (p<0.10) AIA and AIH as well as β-sheet height which all are significantly related to the protein level. Conclusion: Protein structure spectral profiles of by-product form coffee processing could be utilized as potential evaluators to estimate protein related chemical profile and protein metabolic characteristics in ruminant system.

Synthesis of Co(II), Ni(II) and Cu(II) Complexes from Schiff base Ligand and Reactivity Studies with Thermosetting Epoxy Resin

  • Lakshmi, B.;Shivananda, K.N.;Prakash, Gouda Avaji;Rama, Krishna Reddy K.;Mahendra, K.N.
    • Bulletin of the Korean Chemical Society
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    • v.32 no.5
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    • pp.1613-1619
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    • 2011
  • A hybrid thermosetting maleimido epoxy compound 4-(N-maleimidophenyl) glycidylether (N-MPGE) containing Co(II), Ni(II) and Cu(II) ions was prepared by curing N-MPGE and tetradentate Schiff base Co(II), Ni(II) and Cu(II) complexes. The curing polymerization reaction of N-MPGE with metal complexes as curing agents was studied. The cured samples were studied for thermal stability, chemical (acid/alkali/solvent) and water absorption resistance and homogeneity of the cured systems. The tetradentate Schiff base, 3-[(Z)-2-piperazin-1-yl-ethylimino]-1,3-dihydro indol-2-one was synthesized by the condensation of Isatin (Indole-2, 3-dione) with 1-(2-aminoethyl)piperazine (AEP). Its complexes with Co(II), Ni(II) and Cu(II) have been synthesized and characterized by microanalysis, conductivity, Uv-Visible, FT-IR, TGA and magnetic susceptibility measurements. The spectral data revealed that the ligand acts as a neutral tetradentate Schiff base and coordinating through the azomethine nitrogen, two piperazine nitrogen atoms and carbonyl oxygen.

Electroencephalographic Changes Induced by a Neurofeedback Training : A Preliminary Study in Primary Insomniac Patients (뉴로피드백 훈련에 의한 뇌파 변화 연구 : 일차성 불면증 환자에 대한 예비 연구)

  • Lee, Jin Han;Shin, Hong-Beom;Kim, Jong Won;Suh, Ho-Suk;Lee, Young Jin
    • Sleep Medicine and Psychophysiology
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    • v.26 no.1
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    • pp.44-48
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    • 2019
  • Objectives: Insomnia is one of the most prevalent sleep disorders. Recent studies suggest that cognitive and physical arousal play an important role in the generation of primary insomnia. Studies have also shown that information processing disorders due to cortical hyperactivity might interfere with normal sleep onset and sleep continuity. Therefore, focusing on central nervous system arousal and normalizing the information process have become current topics of interest. It has been well known that neurofeedback can reduce the brain hyperarousal by modulating patients' brain waves during a sequence of behavior therapy. The purpose of this study was to investigate effects of neurofeedback therapy on electroencephalography (EEG) characteristics in patients with primary insomnia. Methods: Thirteen subjects who met the criteria for an insomnia diagnosis and 14 control subjects who were matched on sex and age were included. Neurofeedback and sham treatments were performed in a random order for 30 minutes, respectively. EEG spectral power analyses were performed to quantify effects of the neurofeedback therapy on brain wave forms. Results: In patients with primary insomnia, relative spectral theta and sigma power during a therapeutic neurofeedback session were significantly lower than during a sham session ($13.9{\pm}2.6$ vs. $12.2{\pm}3.8$ and $3.6{\pm}0.9$ vs. $3.2{\pm}1.0$ in %, respectively; p < 0.05). There were no statistically significant changes in other EEG spectral bands. Conclusion: For the first time in Korea, EEG spectral power in the theta band was found to increase when a neurofeedback session was applied to patients with insomnia. This outcome might provide some insight into new interventions for improving sleep onset. However, the treatment response of insomniacs was not precisely evaluated due to limitations of the current pilot study, which requires follow-up studies with larger samples in the future.

Generalized Two-dimensional (2D) Correlation Spectroscopy: Principle and Its Applications (일반화된 이차원 상관 분광학: 원리 및 응용)

  • Young Mee Jung;Seung Bin Kim
    • Journal of the Korean Chemical Society
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    • v.47 no.5
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    • pp.447-459
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    • 2003
  • Generalized 2D correlation spectroscopy has been applied extensively to the analysis of spectral data sets obtained during the observation of a system under some external perturbation. It is used in various fields of spectroscopy including IR, Raman, UV, fluorescence, X-ray diffraction, and X-ray absorption spectroscopy (XAS) as well as chromatography. 2D hetero-spectral correlation analysis compares two completely different types of spectra obtained for a system under the same perturbation. Because of the wide range of applications of this technique, it has become one of the standard analytical techniques for the analytical chemistry, physical chemistry, biochemistry, and so on, and for studies of polymers, biomolecules, nanomaterials, etc. In this paper, we will introduce the principle of generalized 2D correlation spectroscopy and its applications that we have studied.

Spanwise coherent structure of wind turbulence and induced pressure on rectangular cylinders

  • Le, Thai-Hoa;Matsumoto, Masaru;Shirato, Hiromichi
    • Wind and Structures
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    • v.12 no.5
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    • pp.441-455
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    • 2009
  • Studying the spatial distribution in coherent fields such as turbulence and turbulence-induced force is important to model and evaluate turbulence-induced forces and response of structures in the turbulent flows. Turbulence field-based coherence function is commonly used for the spatial distribution characteristic of the turbulence-induced forces in the frequency domain so far. This paper will focus to study spectral coherent structure of the turbulence and induced forces in not only the frequency domain using conventional Fourier transform-based coherence, but also temporo-spectral coherence one in the time-frequency plane thanks to wavelet transform-based coherence for better understanding of the turbulence and force coherences and their spatial distributions. Effects of spanwise separations, bluff body flow, flow conditions and Karman vortex on coherent structures of the turbulence and induced pressure, comparison between turbulence and pressure coherences as well as intermittency of the coherent structure in the time-frequency plane will be investigated here. Some new findings are that not only the force coherence is higher than the turbulence coherence, the coherences of turbulence and forces depend on the spanwise separation as previous studies, but also the coherent structures of turbulence and forces relate to the ongoing turbulence flow and bluff body flow, moreover, intermittency in the time domain and low spectral band is considered as the nature of the coherent structure. Simultaneous measurements of the surface pressure and turbulence have been carried out on some typical rectangular cylinders with slenderness ratios B/D=1 (without and with splitter plate) and B/D=5 under the artificial turbulent flows in the wind tunnel.

Structural damage detection through longitudinal wave propagation using spectral finite element method

  • Kumar, K. Varun;Saravanan, T. Jothi;Sreekala, R.;Gopalakrishnan, N.;Mini, K.M.
    • Geomechanics and Engineering
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    • v.12 no.1
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    • pp.161-183
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    • 2017
  • This paper investigates the damage identification of the concrete pile element through axial wave propagation technique using computational and experimental studies. Now-a-days, concrete pile foundations are often common in all engineering structures and their safety is significant for preventing the failure. Damage detection and estimation in a sub-structure is challenging as the visual picture of the sub-structure and its condition is not well known and the state of the structure or foundation can be inferred only through its static and dynamic response. The concept of wave propagation involves dynamic impedance and whenever a wave encounters a changing impedance (due to loss of stiffness), a reflecting wave is generated with the total strain energy forked as reflected as well as refracted portions. Among many frequency domain methods, the Spectral Finite Element method (SFEM) has been found suitable for analysis of wave propagation in real engineering structures as the formulation is based on dynamic equilibrium under harmonic steady state excitation. The feasibility of the axial wave propagation technique is studied through numerical simulations using Elementary rod theory and higher order Love rod theory under SFEM and ABAQUS dynamic explicit analysis with experimental validation exercise. Towards simulating the damage scenario in a pile element, dis-continuity (impedance mismatch) is induced by varying its cross-sectional area along its length. Both experimental and computational investigations are performed under pulse-echo and pitch-catch configuration methods. Analytical and experimental results are in good agreement.

Bit Error Probability Performance of Multi-carrier CDMA System Using M-band Cosine Modulated Filter Bank in the Mobile Radio Channel (M-밴드 코사인변조 필터뱅크를 이용한 멀티캐리어 CDMA 시스템의 이동무선환경에서의 비트오율 성능)

  • Kim, Myoung-Jin
    • Journal of the Korean Institute of Telematics and Electronics S
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    • v.36S no.6
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    • pp.1-8
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    • 1999
  • Multi-carrier CDMA is a transmission scheme where data symbols are spread in the frequency domain with a spread code then transmitted using multiple carriers. It is robust against narrowband interference because of its long symbol duration. However, due to large sidebands of the rectangular window frequency response, subchannels spectrally overlap with neighboring subchannels, which leads to substantial intercarrier interference in a dispersive channel. In this paper, we consider a mult-carrier CDMA system where subchannel spectral containment is achiveved by M-band cosine modulated filter bank (CMFB). In CMFB based MC-CDMA, subchannel spectral containment is achieved using the filter bank of longer impulse response than that of conventional discrete Fourier transform (DFT) filter bank. We show that spectral containment feature of the CMFB based fading channel is analyzed using computer simulations.

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Assessment of statistical sampling methods and approximation models applied to aeroacoustic and vibroacoustic problems

  • Biedermann, Till M.;Reich, Marius;Kameier, Frank;Adam, Mario;Paschereit, C.O.
    • Advances in aircraft and spacecraft science
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    • v.6 no.6
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    • pp.529-550
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    • 2019
  • The effect of multiple process parameters on a set of continuous response variables is, especially in experimental designs, difficult and intricate to determine. Due to the complexity in aeroacoustic and vibroacoustic studies, the often-performed simple one-factor-at-a-time method turns out to be the least effective approach. In contrast, the statistical Design of Experiments is a technique used with the objective to maximize the obtained information while keeping the experimental effort at a minimum. The presented work aims at giving insights on Design of Experiments applied to aeroacoustic and vibroacoustic problems while comparing different experimental designs and approximation models. For this purpose, an experimental rig of a ducted low-pressure fan is developed that allows gathering data of both, aerodynamic and aeroacoustic nature while analysing three independent process parameters. The experimental designs used to sample the design space are a Central Composite design and a Box-Behnken design, both used to model a response surface regression, and Latin Hypercube sampling to model an Artificial Neural network. The results indicate that Latin Hypercube sampling extracts information that is more diverse and, in combination with an Artificial Neural network, outperforms the quadratic response surface regressions. It is shown that the Latin Hypercube sampling, initially developed for computer-aided experiments, can also be used as an experimental design. To further increase the benefit of the presented approach, spectral information of every experimental test point is extracted and Artificial Neural networks are chosen for modelling the spectral information since they show to be the most universal approximators.

Application of Bispectral Analysis to Estimate Nonlinear Acoustic Parameter (음향 비선형 파라미터의 추정을 위한 바이스펙트럼 해석법의 적용)

  • Kim, K.C.;Jhang, K.Y.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.19 no.2
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    • pp.85-92
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    • 1999
  • The fact that material degradation can be evaluated by measuring nonlinear acoustic effect has been proposed by previous studies. The most conventional method to measure nonlinear acoustic effect is to measure the absolute magnitude of fundamental and $2^{nd}$ order harmonic frequency component in the propagated ultrasonic wave. For this aim, power spectral analysis technique has been used widely. However, the power spectral analysis has fatal disadvantage that the gaussian additive noise superimposed in the wave signal remains in the power spectrum domain. Moreover, the magnitude of $2^{nd}$ order harmonic frequency component generated by nonlinear effect is so small that it may be suppressed by the noise remained in the power spectrum. In order to overcome this problem, this paper proposes an alternative method using bispectrum analysis, which can reduce the effect of addictive gaussian noise and. the nonlinear parameter can be obtained more stably. Simulations showed that the proposed method can obtain the value of nonlinear parameter near to the true value in the case of low SNR signal. Also, in order to confirm the usefulness of our method in actual case, we compared the nonlinear parameter obtained by using both of power spectral and bispectral analysis for several specimen intentionally degraded by fatigue load.

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The solar photospheric and chromospheric magnetic field as observed in the near-infrared

  • Collados, Manuel
    • The Bulletin of The Korean Astronomical Society
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    • v.41 no.1
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    • pp.31.4-32
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    • 2016
  • Observing the solar atmosphere with ground-based telescopes in the near-infrared has a number of advantages when compared to classical measurements in visible wavelengths. One of them comes from the magnetic sensitivity of spectral lines, which varies as ${\lambda}_g$, where g is the effective $Land{\acute{e}}$ factor of the transition. This wavelength dependence makes the near-infrared range adequate to study subtle spatial or temporal variations of the magnetic field. Spectral lines, such as the photospheric Fe I $1.5648{\mu}m$ spectral line, with a $Land{\acute{e}}$ factor g=3, have often been used in the past for this type of studies. To study the chromosphere, the Ca II IR triplet and the He I $1.0830{\mu}m$ triplet are the most often observed lines. The latter has the additional advantage that the photospheric Si I $1.0827{\mu}m$ is close enough so that photosphere and chromosphere can be simultaneously recorded with a single detector in a spectrograph. The instrument TIP (Tenerife Infrared Polarimeter) has been continuously operating since 1999 at the 70-cm German VTT of the Observatorio del Teide and has been recently moved to the 1.5-m German GREGOR. During all this time, results have been obtained concerning the nature of the weak photospheric magnetic field of the quiet sun, magneto-acoustic wave propagation, evolution with the cycle of sunspot magnetic fields, photospheric and chromospheric magnetic field in emerging regions, magnetic field in chromospheric structures such as filaments, prominences, flares, and spicules, etc. In this talk, I will review the main results obtained after all these observations and mention the main challenges for the future. With its novel polarization-free design and a complete suite of instruments aimed at simultaneous (imaging and spectroscopic) observations of the solar photosphere and chromosphere, the EST (European Solar Telescope) will represent a major world-wide infrastructure to understand the physical nature of all these phenomena.

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