• 한국미생물·생명공학회지
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• 제18권3호
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• pp.280-285
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• 1990

Biotransformation of progesterone to 11 $\alpha$ -hydroxyprogesterone by growing cells of Rhizopus nigricans was investigated. As the concentration of progesterone increased, the specific growth rate of R. nigricans decreased linearly, and consequently the conversion yield lowered. The hyphae of the microorganism were observed to become thicker, shorter, and more densely branched at high concentrations of progesterone. In order to improve the process productivity, biotransformation was conducted with continuous addition of progesterone. When the substrate was added continuously at a rate of 0.86 g/hr for 30 hrs, overall conversion yield reached upto 56% while a single addition of the same amount of progesterone yielded about 40% eonversion. When additional feeding of glucose was carried out upon its depletion, an improved br'oconversion yield upto 68% was obtained.

• Structural Engineering and Mechanics
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• 제26권3호
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• pp.297-313
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• 2007

A preliminary performance-based seismic design methodology is proposed. The top yield displacement of the system is computed from these of the components, which are assumed constant. Besides, a simple procedure to evaluate the top yield displacement of frames is developed. Seismic demands are represented in the form of yield point spectra. The methodology is general, conceptually transparent, uses simple calculations based on first principles and is applicable to asymmetric systems. To consider a specific situation two earthquake levels, occasional and rare are considered. The advantage of an arbitrary assignment of strength to the different components to reduce eccentricities and improved the torsional response of the system is addressed. The methodology is applied to an asymmetric five story building, and the results are verified by push-over analysis and non linear dynamic analysis.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2005년도 제17회 워크샵 및 추계학술대회
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• pp.299-306
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• 2005

Anaerobic fermentation of food waste (FW) and waste activated sludge (WAS) for hydrogen production was performed in CSTR (Continuous Stirred tank reactor) under various HRTs and volumetric mixing ratio (V/V) of two substrates, FW and WAS. The specific hydrogen production potential of FW was higher than that of WAS. However, pH drop in the CSTR for hydrogen production from FW was higher than that from WAS. The maintenance of desired pH during fermentative hydrogen production is regarded as the most important operation parameter for the stable hydrogen production. Therefore, when the potential of hydrogen production from FW and better buffer capacity of WAS, the proper mixture of FW and WAS for fermentative hydrogen production were considered as a useful complementary substrate. The maximum yield of specific hydrogen production, 140 mL/g VSS, was found at HRT of 2 day and the volumetric mixing ratio of 20:80 (WAS : FW). The spatial distribution of hydrogen producing bacteria was observed in anaerobic fermentative reactor using fluorescent in situ hybridization (FISH) method.

• 대한방사성의약품학회지
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• 제4권2호
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• pp.85-89
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• 2018

We demonstrate a detail protocol for the radiosynthesis of an $^{125}I$-labeled MSTP prosthetic group and its application to the efficient radiolabeling of human serum albumin (HSA). Radioiodination of the precursor (2) was carried out by using $[^{125}I]$NaI and chloramine T as an oxidant at room temperature for 15 min. After HPLC purification of the crude product, the purified $^{125}I$-labeled MSTP ($[^{125}I]1$) was obtained with high radiochemical yield ($73{\pm}5%$, n = 3) and excellent radiochemical purity (>99%). Site-specific reaction between ($[^{125}I]1$) and HSA gave the $^{125}I$-labeled human serum albumin ($[^{125}I]3$) with more than 99% of radiochemical yield as determined by radio-thin-layer chromatography (radio-TLC). These results clearly demonstrate that the present radiolabeling method will be useful for the efficient and convenient radiolabeling of thiol-bearing biomolecules.

• KSBB Journal
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• 제9권4호
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• pp.418-427
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• 1994

For the production of ${\alpha}$-amylase cloned from Bacillus licheniformis expressed in E. coli, cultivating factors including the concentrations of glucose, maltose and acetic acid were investigated. The results were as follows: 1) Maximum ${\alpha}$-amylase yield and maximum specific production rate obtained from glucose source were better than those achieved from maltose source. 2) The optimum production yield of ${\alpha}$-amylase was obtained at 1.0ml/$\ell$ or less of initial acetic acid concentration.

• 한국산림과학회지
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• 제75권1호
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• pp.55-66
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• 1986

개암나무 10품종(品種)의 각(各) 특성(特性)에 대한 일반조합능력(一般組合能力)이 좋은 개체(個體)와 특수조합능력(特殊組合能力)이 좋은 교배조합(交配組合)을 선발(選拔)하기 위(爲)하여 완전대조교배(完全對照交配)를 실시(實施)하였다. ha 당(當) 인중생산량(仁重生産量)에서 일반조합능력(一般組合能力)이 가장 좋은 개체(個體)는 "Barcelona" 이었으며 특수조합능력(特殊組合能力)이 좋은 교배조합(交配組合)은 Kara${\times}$Badem, Badem${\times}$Barcelona, Sivri${\times}$Barcelona, Sirri${\times}$Barcelona, Palaz${\times}$Barcelona, Tombul${\times}$Kara, Barcelona${\times}$Sivri, Hukuken 2호(號)${\times}$Hukuken 3호(號), Hukuken 3호(號)${\times}$Hukuken 2호(號)이다. 교배조합간(交配組合間)의 결실율(結實率), 과우(果友)두께, 입중(粒重), 인중(仁重), 인중비(仁重比), 본당(本當) 혹은 ha당(當) 인중생산량(仁重生産量)의 특성(特性)에 대(對)하여도 비교분석(比交分析) 하였다.

• 한국식품과학회지
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• 제27권5호
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• pp.673-679
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• 1995

Valencia 오렌지로부터 추출한 펙틴에스테라제(pectinesterase, PE) 중에서 가열처리하지 않은 PE 조효소를 CM-Sephadex C-50, Phenyl Sepharose CL-4B와 CM-Biogel A의 chromatography를 차례로 수행하여 내열성이 약한 TLPE(thermolabile pectinesterase)와 내열성이 강한 TSPE(thermostable pectinesterase)를 분리 정제하였다. PE 중에서 가열처리한 PE 조효소에서는 위와 같은 정제과정으로 TSPE 만을 분리하였다. TLPE는 비활성 1,005 units/mg이고 35배로 정제된 것을 13.6% 회수할 수 있었으며, 반면에 가열하지 않고 분리한 TSPE는 비활성 3,115 units/mg이고 100.5배로 정제된 것을 1.5% 얻을 수 있었으나 가열하여 분리한 TSPE는 비활성 1,803 units/mg이고 가열처리한 조효소가 140배로 정제된 것을 15.4% 얻을 수 있었다.

• 한국물환경학회지
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• 제23권5호
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• pp.650-658
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• 2007

The Universal Soil Loss Equation (USLE) has been used in over 100 countries to estimate potential long-term soil erosion from the field. However, the USLE estimated soil erosion cannot be used to estimate the sediment delivered to the stream networks. For an effective erosion control, it is necessary to compute sediment delivery ratio (SDR) for watershed and sediment yield at watershed outlet. Thus, the Sediment Assessment Tool for Effective Erosion Control (SATEEC) was developed to compute the sediment yield at any point in watershed. In this study, the SATEEC was applied to the Sudong watershed, Chuncheon Gangwon to compare the sediment yield using area-based sediment delivery ratio (SDRA) and slope-based sediment delivery ratio (SDRS) at watershed outlet. The sediment yield using the SDRA by Vanoni, SYA and the sediment yield using the SDRS by Willams and Berndt, SYS were compared for the same sized watersheds. The 19 subwatersheds was 2.19 ha in size, the soil loss and sediment yield were estimated for each subwatershed. Average slope of main stream was about 0.86~3.17%. Soil loss and sediment yield using SDRA and SDRS were distinguished depending on topography, especially in steep and flat areas. The SDRA for all subwatersheds was 0.762, however the SDRS were estimated in the range of 0.553~0.999. The difference between SYA and SYS was -79.74~27.45%. Thus site specific slope-based SDR is more effective in sediment yield estimation than area-based SDR. However it is recommended that watershed characteristic need to be considered in estimating yield behaviors.

• 한국식품과학회지
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• 제31권4호
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• pp.1011-1016
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• 1999

식물의 세포벽 모형에 근거하여 고온고압의 조건하에서 기계적인 전단웅력을 식물 세포벽에 가할 경우 수용성 다당류를 효과적으로 분리할 수 있다는 가설에 근거하여 사과박으로부터 펙틴을 추출하기 위해 압출처리법을 도입하였다. L/D비가 20: 1인 동방향완전맞물림형 이축압출기를 이용하여 압출기의 축회전수, 원료의 공급률 및 원료의 수분함량을 공정변수로 하고 압출기의 비기계에너지를 시스템의 매개변수로 하여 압출처리에 의한 수용성 다당류의 수율, 평균 분자량 및 galacturonic acid 함량을 분석한 후, 각각의 모형을 개발하였다. 펙틴의 수율과 품질을 동시에 비교해 볼때, 비기계에너지를 증가시키면 펙틴의 수율이 향상되는 것으로 나타났으나, 수율을 향상시키는 압출조건은 품질의 저하를 초래하는 것으로 나타났다. 비기계에너지가 191 kWh/ton인 고강도의 추출조건에서 수율은 20.1%로 나타나 기존의 산처리법에 의한 14.4% 수율보다 월등히 향상된 수율을 얻을 수 있었다. 한편, 비기계에너지가 96 kWh/ton인 저강도의 추출조건에서는 분자량이 240,000인 펙틴을 얻을 수 있어 산처리시 분자량 181,000의 펙틴보다 훨씬 양질의 펙틴을 얻을 수 있었다. 따라서, 목표로 하는 품질의 펙틴을 생산할 수 있으면서 최고의 수율을 얻을 수 있는 압출조건을 설정해야 하며, 이를 위해서 본 연구에서 제시한 펙틴의 수율 및 품질 모형을 이용할 수 있다.

• Plant Breeding and Biotechnology
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• 제5권4호
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• pp.371-389
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• 2017

Supplying sufficient rice to growing populations is a global challenge. Hybrid indica rice varieties exploiting heterosis have increased yields, but inter-subspecific crosses between indica and japonica varieties are hampered by sterility. Examination and genetic understanding of yield heterosis in indica/japonica crosses addressing yield barriers are basic requirements. In this study, QTLs for heterosis of yield traits were identified in indica-japonica recombinant inbred lines (RILs) using a total of 178 RILs originating from Dasanbyeo (indica) ${\times}$ TR22183 (japonica) (DT-RILs) and their backcrossed populations. Nine of sixty-six major quantitative trait loci (QTLs) identified in DT-RILs exhibited heterosis. Heterosis QTLs clustered with other traits on chromosomes 1, 4, and 8, and clusters were conserved between different RILs. The clusters contained several known yield enhancement genes/QTLs. Specific heterotic allele combinations contributed to four major heterosis QTLs, particularly for panicle and spikelet number traits. Heterosis for yield and yield-related traits was explained by the harmonized effects of overdominance, dominance, and epistatic interactions in inter-subspecific breeding populations.