• The Plant Pathology Journal
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• v.24 no.2
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• pp.101-111
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• 2008

The fungal genus Alternaria is comprised of many saprophytic and endophytic species, but is most well known as containing many notoriously destructive plant pathogens. There are over 4,000 Alternaria/host associations recorded in the USDA Fungal Host Index ranking the genus 10th among nearly 2,000 fungal genera based on the total number of host records. While few Alternaria species appear to have a sexual stage to their life cycles, the majority lack sexuality altogether. Many pathogenic species of Alternaria are prolific toxin producers, which facilitates their necrotrophic lifestyle. Necrotrophs must kill host cells prior to colonization, and thus these toxins are secreted to facilitate host cell death often by triggering genetically programmed apoptotic pathways or by directly causing cell damage resulting in necrosis. While many species of Alternaria produce toxins with rather broad host ranges, a closely-related group of agronomically important Alternaria species produce selective toxins with a very narrow range often to the cultivar level. Genes that code for and direct the biosynthesis of these host-specific toxins for the Alternaria alternata sensu lato lineages are often contained on small, mostly conditionally dispensable, chromosomes. Besides the role of toxins in Alternaria pathogenesis, relatively few genes and/or gene products have been identified that contribute to or are required for pathogenicity. Recently, the completion of the A. brassicicola genome sequencing project has facilitated the examination of a substantial subset of genes for their role in pathogenicity. In this review, we will highlight the role of toxins in Alternaria pathogenesis and the use of A. brassicicola as a model representative for basic virulence studies for the genus as a whole. The current status of these research efforts will be discussed.

• Korean Journal of Plant Resources
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• v.24 no.2
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• pp.214-235
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• 2011

The field survey to investigate the flora in Sogwang-ri, Uljin-gun, was carried out 10 times, from April 2009 to September 2010, and The results summarized as 430 taxa, 89 families, 253 genera, 367 species, 3 subspecies, 53 varieties, and 7 forms. Among these, 13 taxa were recorded as Korean endemic plant species and 11 taxa as rare plant species, which applied by the Korean Forest Service. Furthermore, 54 taxa were listed as specific plant species based on phytogeographical points of view and based on the list of those plants approved for delivery overseas, 17 taxa were recorded as such in the investigated area. There were 15 taxa identified as naturalized plants, and their naturalization ratio was found to be 3.4%. Among the 430 taxa, 178 taxa (40.9%) were categorized as edible plants, 145 taxa (33.7%) as medicinal plants, 114 taxa (26.5%) as pasture plants, 63 taxa (14.6%) as ornamental plants, 15 taxa (3.4%) as timber plants, 11 taxa (2.5%) as fiber plants, and 4 taxa (0.9%) as industrial plants.

• Korean Journal of Environment and Ecology
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• v.26 no.5
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• pp.635-653
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• 2012

Vascular plants of Oruem in region of Jeju-City were investigated 454 taxa totally; 116 families, 301 genera, 359 species, 3 subspecies, 78 varieties and 14 forms. In the studied Oruems in region of Jeju-City within altitude 800m, evergreen tree forests does not range and a lot of Pines thunbergii forest and Cryptomeria japonica and Chamaecyparis obtusa plantation forest were. The endemic species of Oruem in region of Jeju-City were 14 taxa such as Asarum maculatum, Hepatica insularis, Cardamine glechomifolia etc. The floristics degree categorized by the specific distribution of plant species were total 116 Taxa, that is, V grade species Magnolia kobus, Aconitum japonicum subsp. napiforme, Ligularia taquetii etc, 6 kinds be, IV grade species Rubus ribisoideus, Ligustrum salicinum, Carex macrandrolepis etc, 16 kinds be, III grade species Illicium anisatum, Hydrangea petiolaris, Rubus schizostylus etc, 37 kinds be, II grade species Pseudostellaria coreana, potentilla dickinsii, Euonymus pauciflorus etc, 6 kinds and I grade species Sphenomeris chinensis, Pteris multifida, Machilus thunbergii etc, be. 51 kinds were confirmed. Rare and Endemic Plant Species of Korea Forest Service were 11 taxa such as Asarum maculatum, Aconitum japonicum subsp. napiforme and Magnolia kobus etc. Naturalized plant were confirmed 31 taxons with Rumex acetosella, Hypochaeris radicata and Sisyrinchium atlanticum etc.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.2
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• pp.66-74
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• 2008

In recent years, the view centered conservation for eco-system is getting important rather than development from human oriented perspective. As a result, the concept "Sustainable development", that is seeking harmony between conservation and development, appeared so that we researched distribution and status of vascular plants a site of project in the Bukkyungnam substation. The main findings from the study is as follows. The vascular plants a site of project in the Bukkyungnam substation area was listed 435 taxa; 93 families, 269 genera, 377 species, 1 subspecies, 51 varieties and 6 forms. Specific plant species by floral region were total 25 taxa; Acer palmatum (Planted species), Asperula lasiantha in Class III, Caryopteris incana, Cirsium schantarense in Class II, 21 taxa (Dryopteris erythrosora, Chloranthus japonicus, Salix glandulosa, Alnus hirsuta, Quercus variabilis, Ulmus parvifolia, Clematis patens, Rubus hirsutus, Euphorbia pekinensis, Cayratia japonica, Grewia biloba var. parviflora, Vaccinium oldhami, Microstegium japonicum, Hosta capitata, Polygonatum falcatum, etc.) in Class I. Based on the list of Korean endemic plants, 10 taxa were recorded; Cephalotaxus koreana, Populus tomentiglandulosa (Planted species), Indigofera koreana, Forsythia koreana (Planted species), Paulownia coreana (Planted species), Asperula lasiantha, Weigela subsessilis, Adenophora triphylla var. hirsuta, Carex okamotoi, Lilium amabile and based on the list of the rare plants by the Forest ResearchInstitute, 2 taxa were recorded in the studied areas; Lilium callosum (Preservation priority order; No. 191), Aristolochia contorta (No. 151). The naturalized plants in this site were 13 families, 22 genera, 28 taxa and naturalization rate was 6.4% of all 435 taxa vascular plants.

• Korean Journal of Veterinary Research
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• v.55 no.2
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• pp.105-110
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• 2015

A real-time PCR assay using hybridization probe (HybProbe) has been developed to detect Brucella (B.) melitensis strains. The primer and HybProbe sets were designed based on the gap gene of chromosome I with a specific single nucleotide polymorphism of B. melitensis. Specificity of the assay was confirmed by comparison to reference Brucella species and other related strains. In the melting curve analysis, B. melitensis generated a peak at $67^{\circ}C$ unlike those for other Brucella species observed at $61^{\circ}C$. Sensitivity of the assay for B. melitensis ranged from 20 ng to 200 fg of genomic DNA. The ability to identify 94 Mongolian B. melitensis isolates using the real-time PCR assay was identical to that of classical biotyping methods and differential multiplex PCR. These data showed that this new molecular technique is a simple and quick method for detecting B. melitensis, which will be important for the control and prevention of brucellosis.

• The Plant Pathology Journal
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• v.24 no.1
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• pp.17-23
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• 2008

A total of 33 isolates of Colletotrichum species obtained from pepper, apple, and strawberry in 2001 and 2002 were identified based on mycological characteristics, responses to fungicides carbendazim and the mixture of carbendazim and diethofencarb, and nucleotide sequence analysis of internal transcribed spacer (ITS) regionMost of the Colletotrichum isolates from pepper could be identified as C. acutatum. The pepper isolates produced grey white mycelia that gradually changed to dark gray. The conidia were variable in size, and almost cylindrical in shape with at least one rounded end. They could grow on PDA amended with carbendazim or with the mixture of carbendazim and diethofencarb at $10{\mu}g/ml$, to which the isolates from apple and strawberry were very sensitive. A part of the ITS regions from the Colletotrichum isolates was amplified with the specific primers designed for C. acutatum (Ca1-1) or C. gloeosporioides (Cg1-3). A primer pair of Ca1-1 and a universal primer (ITS4) amplified a 496-bp DNA fragment from all of the pepper isolates examined and one apple isolate. Taken together, it is conclusive that the Colletotrichum isolates causing the typical lesion of anthracnose on pepper fruits are C. acutatum.

• Journal of Plant Biology
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• v.28 no.3
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• pp.217-224
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• 1985

Because Ceramium fastigiatum Harvey (1834) is a later homonym of C. fastigiatum Roth (1806), a quite different plant from the former, it becomes illegitimate and must be rejected under the Article 64 of International Code of Botanical Nomenclature. For this reason, we suggest to give a new name, Ceramium fastigiramosum Boo et Lee, to the former species, keeping the original specific epithet‘fastigiatum’. The morphology of vegetative and reproductive structures is re-examined. The life history is confirmed as a Polysiphonia-type in laboratory culture.

• The Plant Pathology Journal
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• v.20 no.4
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• pp.302-307
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• 2004

Three pairs of specific primers were developed for rapid and precise RT-PCR detection of three seed-transmissible viruses, namely Peanut clump virus (PCV, Pecluvirus), White clover mosaic virus (WCIMV, Potexvirus) and Carrot red leaf virus (CaRLV, Luteovirus). Each primer set was found in conserved region through multiple sequence alignment in the DNAMAN. Total nucleic acids extracted from PCV-, WCMV-, and CaRLV-infected seeds and healthy plants were used for RT-PCR detection using each virus-specific primer, Sizes of PCV, WCIMV, and CaRLV PCR products were 617bp (PCV-uni5 and PCV-uni3 primers), 561bp (WCMV-CP5 and WCMV-CP3 primers), and 626bp (CL1-UP and CL2-DN primers); which corresponded to the target sizes. Nucleotides sequences of each amplified cDNA were confirmed which belonged to the original virus. This study suggests that these virus-specific primer sets can specifically amplify viral sequences in infected seeds. Thus, they can be used for specific detection of three viruses (PCV, WCMV and CaRLV) from imported seed samples for plant quarantine service.

• Journal of Plant Biotechnology
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• v.49 no.1
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• pp.30-38
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• 2022

Solanum brevicaule is one of the tuber-bearing wild Solanum species. Because of its resistance to several important pathogens infecting potatoes during cultivation, it can be used for potato breeding. However, the fact that S. brevicaule used in this study has an EBN value of two causes the sexual reproduction barriers between the species and cultivated potatoes. In this study, specific markers for discriminating S. brevicaule from other Solanum species were developed on the basis of the results of sequence alignments with the whole chloroplast genomes of S. brevicaule and seven other Solanum species. The chloroplast genome of S. brevicaule was completed by next-generation sequencing technology described in other recent studies. The total sequence length of the chloroplast genome of S. brevicaule is 155,531 bp. Its structure and gene composition are similar to those of other Solanum species. Phylogenetic analysis revealed that S. brevicaule was closely grouped with other Solanum species. BLASTN search showed that its genome sequence had 99.99% and 99.89% identity with those of S. spegazzinii (MH021562) and S. kurtzianum (MH021495), respectively. Sequence alignment identified 27 SNPs that were specific to S. brevicaule. Thus, three PCR-based CAPS markers specific to S. brevicaule were developed on the basis of these SNPs. This study will facilitate in further studies on evolutionary and breeding aspects in Solanum species.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.1
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• pp.44-56
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• 2007

The flora was summarized as 195 taxa; 73 families, 138 genera, 168 species, 23 varieties and 4 forms in Jangsoo-ri windbreak forest and 151 taxa; 63 families, 118 genera, 131 species, 18 varieties and 2 forms in Pyeongsa-ri windbreak forest. Based on the list of the rare plants by the Forest Research Institute, 3 taxa and based on the list of Korean endemic plant 4 taxa were recorded in the studied areas; Puccinellia coreensis (Gramineae), Asarum maculatum (Aristolochiaceae), Prunus yedoensis (Rosaceae), Koelreuteria paniculata (Sapindaceae), Adenophora triphylla var. hirsuta (Campanulaceae). The Specific Plant Species which is categorized by the Ministry of Environment, Korea, was summarized as 23 taxa for the 1 degree of 17 taxa, 3 degree of 5 taxa, 5 degree of 1 taxa in Jangsoo-ri windbreak forest and 22 taxa for the 1 degree of 18 taxa, 3 degree of 3 taxa, 5 degree of 1 taxa in Pyeongsa-ri windbreak forest. From the enlisted the flora surveyed, the naturalized plants were 17 taxa in Jangsoo-ri windbreak forest and 19 taxa in Pyeongsa-ri windbreak forests with the Naturalization rate was 8.7 percent and 12.6 percent, respectively. In Jangsoo-ri windbreak forest, there has been occurred many man-made impair and development without legal institution and control. So, it needs in haste institutional conservation measures and systemic database construction about function and necessity of windbreak forest. In Pyeongsa-ri windbreak forest, recently many people visited here. So, if we make eco-tour place connecting nearby coast and windbreak forest, it will contribute resident's economic profit and opportunity, concerns about windbreak forest, and necessity of conservation measures. Recently, the windbreak forests in Jangsoo-ri and Pyeongsa-ri should be bereaved windbreak forest of function by the human activities.