• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2004년도 춘계학술발표대회
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• pp.229-229
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• 2004

The identification of embryo-specific genes would provide insights into early embryonic development. However, the current methods employed to identify the genes that are expressed at a specific developmental stage are labor intensive and suffer from high rates of false positives. Here we employed a new and accurate reverse transcription-polymerase chain reaction (RT-PCR) technology that involves annealing control primers (ACPs) to identify the genes that are specifically or prominently expressed in bovine early blastocysts and hatched blastocysts produced in vitro. (omitted)

• Journal of Microbiology and Biotechnology
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• 제20권9호
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• pp.1295-1299
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• 2010

Recently, recombinant Streptomyces venezuelae has been established as a heterologous host for microbial production of flavanones and stilbenes, a class of plant-specific polyketides. In the present work, we expanded the applicability of the S. venezuelae system to the production of more diverse plant polyketides including flavones and flavonols. A plasmid with the synthetic codon-optimized flavone synthase I gene from Petroselium crispum was introduced to S. venezuelae DHS2001 bearing a deletion of the native pikromycin polyketide synthase gene, and the resulting strain generated flavones from exogenously fed flavanones. In addition, a recombinant S. venezuelae mutant expressing a codon-optimized flavanone $3{\beta}$-hydroxylase gene from Citrus siensis and a flavonol synthase gene from Citrus unshius also successfully produced flavonols.

• Journal of Dental Anesthesia and Pain Medicine
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• 제17권1호
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• pp.55-59
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• 2017

Digital dentistry has influenced many dental procedures, such as three-dimensional (3D) diagnosis and treatment planning, surgical splints, and prosthetic treatments. Patient-specific protective appliances (PSPAs) prevent dental injury during endotracheal intubation. However, the required laboratory work takes time, and there is the possibility of tooth extraction while obtaining the dental impression. In this technical report, we utilized new digital technology for creating PSPAs, using direct intraoral scanners and 3D printers for dental cast fabrication.

• 한국유체기계학회 논문집
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• 제14권6호
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• pp.26-34
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• 2011

Waterhammer and slamming phenomena can occur when power is cut off due to reversal flow in pipeline and sudden close of check valve. Therefore analysis of reversal flow time, which means the time of reversal flow in pipeline due to pumping station blackout, is needed to protect facilities from waterhammer economically and efficiently. However systematic study on reversal flow time has not been done yet. So theory of reversal flow time analysis is proposed and verified with experiment using several parameters like pump specific speed, motor pole number, and characteristic curve of pipeline in this study.

• 한국어병학회지
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• 제21권3호
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• pp.181-188
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• 2008

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used to detect and identify four fish viruses, fish iridovirus, viral hemorrhagic septicaemia virus (VHSV), viral nervous necrosis virus (VNNV), hirame rhabdovirus (HRV). Four viruses were detected by PCR with each specific primers. Identification of iridovirus was achieved by digesting the PCR amplified fragment with a restriction enzyme ApaⅠ. It was possible to distinguish positive from false positive PCR amplicons of VHSV by RFLP of PstⅠ or HindⅢ restriction enzymes. VNNV was identified using RFLP of BamHⅠrestriction enzyme and HRV was identified by XbaⅠ restriction enzyme. This approach can be used for more rapid, simple and specific diagnosis of fish viral diseases.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2005년도 International Symposium & conference of the Plant Resources Society of Korea
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• pp.181-181
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• 2005

• 한국실내디자인학회:학술대회논문집
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• 한국실내디자인학회 2004년도 추계학술발표대회 논문집
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• pp.76-79
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• 2004

The purpose of this study is to suggest the specific design guidelines for major physical elements like colors, patterns or shapes which can produce the place identity enhancing wayfinding affordability. The research consists with theoretical studies. From the precedent researches, the main design principles enhancing wayfinding affordability were defined and then the list and the nature of the major physical interior elements were drawn, based on them. The result presented that the specific design guidelines as well as design principles could be focused on the categories like generating landmarks, introducing color codes, floor patterns, and wall decorating. And dealing them to promote place identity, were shown as useful in articulating the pathway and indicating spatial orientation. Design guidelines as the result of the research, could be easily and directly applied on the real public spaces as a visual guidance enhancing navigability(the ability to recover position and orientation) to make up the wayfinding weakness.

• 한국동물위생학회지
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• 제35권4호
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• pp.289-294
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• 2012

Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Infections with Mycoplasma species can cause a variety of problems in living organisms and in vitro cell cultures. In this study, we investigated the usefulness of a genus-specific consensus PCR analysis method to detect Mycoplasma species. The developed consensus primer pairs MycoF and MycoR were designed specifically to amplify the 16S ribosomal RNA gene (rRNA) of Mycoplasma species by the optimized PCR system. The developed consensus PCR system effectively amplified 215 bp of Mycoplasma genus-specific region of 16S rRNA. In conclusion, we recommend this consensus PCR for monitoring Mycoplasma species in animals, human and cell culture system.

• 영어어문교육
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• 제17권2호
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• pp.115-137
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• 2011

The tourism industry has attained remarkable growth, and the need for professional Tourism English education has increased. Universities of online education can offer an environment for education to both job applicants and laymen who are interested in Tourism English. Tourism English belongs to English for Specific Purposes, which reflects the needs of specific area. The aim of this study is to propose improvements in classes design for online Tourism English instruction. The results of a needs analysis conducted on 160 Korean online university students suggest that online Tourism English class should be different from a traditional classroom-based one in regards to aims, contents, and methods. Online Tourism English class should not only focus on English for specific purposes, but also include more generalized topics. This comes as a result of the diverse backgrounds of online students. The results suggest that extralinguistic elements, such as culture and etiquette differences among English-speaking countries, become more interesting when introduced using pictures, videos, animations, etc. Additionally, SMS or emails can be utilized to raise students' motivation for online Tourism English class.

• Bulletin of the Korean Chemical Society
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• 제32권5호
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• pp.1507-1510
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• 2011

In this study, mercury vapor adsorption behaviors for some kinds of porous materials having various pore structures were investigated. The specific surface area and pore structures were studied by BET and D-R plot methods from $N_2$/77 K adsorption isotherms. It was found that the micropore materials (activated carbons, ACs) showed the highest mercury adsorption capacity. In a comparative study of mesoporous materials (SBA-15 and MCM-41), the adsorption capacity of the SBA-15 was higher than that of MCM-41. From the pore structure analysis, it was found that SBA-15 has a higher micropore fraction compared to MCM-41. This result indicates that the mercury vapor adsorptions can be determined by two factors. The first factor is the specific surface area of the adsorbent, and the second is the micropore fraction when the specific surface areas of the adsorbent are similar.