• 한국환경과학회지
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• 제10권3호
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• pp.225-231
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• 2001

The fish species collected in the Keumho River basin are 42 species 31 genera belonging to 15 Families. This report was investigated for the evaluation of water quality in the Keumho River system which is a tributary of Nakdong River in Korea on september in 1999. The fishes collected were 42 species, 31 genera belonging to 15 Families. The dominant species were 5 species; Zacco platypus, Zacco temmincki, Squalidus chankaensis tsuchigae, Moroco oxycephalus, Squalidus gracilis majimae, and 8 species; Hemibarbus longirostris, Pseudogobio esocinus, Culter brevicauda, Cobitis rotundicaudata, Pseudobagrus fulvidraco, Pungitius sinensis kaibarae, Monopterus albus, Channa argus were rare species. The relationship among the GPI, EC and BOD by the organic pollutants were over 0.9. The group pollution index(GPI) was lowest at St. 1(0.85) and highest at St. 1(0.85) and highest at St. 5(2.33). The water quality of the Keumho river divided into 3 parts; the water of upper reaches in river(St. 1) was 1st class(oligotrophic condition), middle parts(St. 2, 3, 4) were 3rd class($\alpha$-mesosaprobic condition) and lower part(St. 5) was 4th class(Polysaprobic condition) as the source of tap water, respectively. And the tributary which are the Sinryeong Stream(St. 6), the Sincheon(St. 7) and the Donghwa Stream(St. 9) in Keumho river were 2nd class as the source of tap water. The results in this study was represented same patterns as the result by the use of indicator species like as algae and invertebrates for the discrimination of water quality. So, some freshwater fish species can be use applicant for the discrimination of water quality.

• 대한수의학회지
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• 제39권3호
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• pp.513-522
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• 1999

DNA hybridization assay using probes prepared from liver was carried out to identify species characterization of the domestic animals. Gel electrophoresis showed that the target DNA extracted from raw muscle were 1kb and uniform pattern while fragments size of heated muscle were irregular. Hybridization was performed by adding 200ng/ml probe in hybridization solution and incubating for 12 hours at $68^{\circ}C$. To obtain good discrimination, applied washing buffer and washing step differently depending on the species. The probes of pig, horse and dog formed the specific hybrids with each target DNA respectively. Although cross reaction was detected in cattle, goat and sheep but signal intensity among these species made the discrimination possible each other. Such pattern was the same in the cases of chicken, turkey and duck. The hybridization pattern of heated muscle was similar to that of raw muscle in general, but the signal intensity was inferior to that of raw muscle. Species identification between closely related animal species, hybridized using the target DNA of such closely related animal species as a blocking agent, remarkable increase of discrimination from the evident decrease of non specific reaction compared with the control group. In addition, in the admixture where certain meat was included in the beef, pork, chicken meat, we could find whether any unjust meat was admixed or not. In this case, detection limit of certain meat in admixture was 1%.

• 한국자원식물학회지
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• 제20권3호
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• pp.247-250
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• 2007

This study was performed to investigate the discrimination of Angelica gigas, Angelica acutiloba and Angelica sinensis on the treatment of chromaticity and colorfastness. Angelica gigantis root has been used as a Korean traditional medicine for the treatment of woman disease. Natural dyes give us many great benefits, including diversified color, but no pollution. These studies were carried out acetate iron, dichloride copper and alum with a mordant to ramie fabric. The ramie fabric was dyed with Angelica gigas, Angelica acutiloba and Angelica sinensis. The results of experiment showed as follows: In discrimination by dyeing, the colors of Angelica acutiloba and Angelica sinensis were very similar, but that of Angelica gigas was different. There were no differences among colors of materials using non-mordant. But dyeing with iron acetate and copper dichloride were showed dark in Angelica gigas than other angelica species.

• 대한본초학회지
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• 제39권3호
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• pp.23-35
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• 2024

Objective : In this study, we performed quantitative comparison on the content of 10 marker compounds in cinnamon barks from different species and found chemical discrimination between genuine Cinnamomum cassia and other Cinnamomum species (Non C. cassia). Methods : Cinnamon bark samples were extracted using the ultrasonication in 100% methanol for 30 minutes. The samples were analysed using high-performance liquid chromatography with statistical analysis. Results : The analytical method developed in this study met all validation criteria and was applied to the quantification of the 10 marker compounds in cinnamon bark samples. The major chemical discrimination of C. cassia were identified as low content of epicatechin and eugenol, and high contents of benzaldehyde, cinnamaldehyde and cinnamic acid compared to other Non C. cassia samples. Especially, among other compounds, the content of cinnamaldehyde was the highest in the C. cassia and Non C. cassia samples. The result of principal component analysis showed that the samples of C. cassia and Non C. cassia were clearly differentiated via benzaldehyde, cinnamaldehyde, cinnamic acid, eugenol, and epicatechin, which influenced on clustering C. cassia and Non C. cassia samples. Conclusion : C. cassia and Non C. cassia samples were chemically discriminated using the quantitative HPLC analysis. Based on this, it is possible to control the quality of herbal medicines containing Cinnamomi Cortex. It is necessary to further improve the accuracy of discrimination between C. cassia and Non C. cassia species to evaluate cinnamon bark quality.

• 동의생리병리학회지
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• 제19권4호
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• pp.1046-1049
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• 2005

Acanthopanx senticosus (Araliaceae) is a long-lived woody species primarily distributed throughout East Asia. This species is regarded as medically and ecologically important woody plants in Korea. To identify the variation of the RAPD patterns between domestic and foreign A. senticosus species, 22 random primers were applied to Korean A. senticosus and A. senticosus for. inermis, Chinese and Russian A. senticosus. Six primers of them could be used to discriminate the origins and 58 polymorphisms among 92 scored DNA fragments. Six bands are specific for Korean A. senticosus and A. senticosus for. inermis. Especially, three primers, OPD04, OPD11 and OPE10, were useful to differentiate between domestic and foreign Acanthopanax species. RAPD analysis was a useful method to discriminate among A. senticosus populations or accessions and Korean accessions are distinct genetically.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2000년도 공동 춘계학술대회
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• pp.63.2-63
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• 2000

No Abstract, See Full Text

• Journal of Microbiology and Biotechnology
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• 제32권8호
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• pp.1011-1016
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• 2022

Bacillus subtilis is a useful bacterium in the food industry with applications as a starter strain for fermented food and as a probiotic. However, it is difficult to discriminate B. subtilis from other Bacillus species because of high phenotypic and genetic similarity. In this study, we employed five previously constructed multilocus sequence typing (MLST) methods for the discrimination of B. subtilis from other Bacillus species and all five MLST assays clearly distinguished B. subtilis. Additionally, the 17 housekeeping genes used in the five MLST assays also clearly distinguished B. subtilis. The pyruvate carboxylase (pyrA) and shikimate dehydrogenase (aroE) genes were selected for the discrimination of B. subtilis because of their high number of polymorphic sites and the fact that they displayed the lowest homology among the 17 housekeeping genes. Specific primer sets for the pyrA and aroE genes were designed and PCR products were specifically amplified from B. subtilis, demonstrating the high specificity of the two housekeeping genes for B. subtilis. This species-specific PCR method provides a quick, simple, powerful, and reliable alternative to conventional methods in the detection and identification of B. subtilis.

• 대한본초학회지
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• 제24권4호
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• pp.115-120
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• 2009

Objectives : Due to the morphological similarity and frequent occurrence of intermediate forms as well as morphological variations of aerial part, the correct identification between Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma is very difficult. To develop a reliable method for correct identification and improving the quality standards of Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma, we analyzed RAPD and developed SCAR marker. Methods : To amplify target DNA at the genomic level, 32 Operon 10-mer random primers were applied with four Rheum species, R. officinale, R. palmatum, R. tanguticum and R. undulatum. The nucleotide sequences were determined and species-specific primers were prepared depending on the species-specific RAPD amplicons after subcloned into the pGEM-Teasy vector. To develop the SCAR markers, species-specific PCR amplification and multiplex-PCR were carried out using the single species-specific primer pairs and combinations of them, respectively. Results : We used RAPD analysis of four Rheum plant species to obtain several species-specific RAPD amplicons. From nucleotide sequences of these RAPD amplicons, we developed two SCAR markers that amplified 314 bp and 390 bp DNA fragments in only R. undulatum but not in R. officinale, R. palmatum, R. tanguticum and R. undulatum, for distinguishing Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma. Furthermore, we established SCAR markers for the simultaneous discrimination of the three species within a single reaction by using multiplex-PCR. Conclusions : These genetic markers can be used for the efficient discrimination of plants species and commercial herbal medicines between Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma, to ultimately prevent indiscriminate distribution and prescription of these herbal medicines.

• International Journal of Oral Biology
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• 제38권1호
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• pp.29-36
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• 2013

Mitis group streptococci (MGS) were classified based on the nucleotide sequences 16S rRNA gene (16S rDNA) and comprised 13 Streptococcus species. However, 16S rDNA homogeneity among MGS was too high to discriminate between clinical strains at the species level, notably between Streptococcus mitis, Streptococcus oralis, Streptococcus pneumoniae, and Streptococcus pseudopneumoniae. The purpose of this study was to discriminate between 37 strains of MGS isolated from Korean oral cavities using phylogenetic analysis of the DNA-dependant RNA polymerase beta-subunit gene (rpoB). 16S rDNA and rpoB from clinical strains of MGS were sequenced using the dideoxy chain termination method and analyzed using MEGA version 5 software. The resulting phylogenetic data showed that the rpoB sequences could delineate clinical strains of MGS at the species level. Phylogenetic analysis of rpoB is therefore a useful approach for identifying MGS at the species level.

• Journal of Plant Biotechnology
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• 제31권1호
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• pp.1-6
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• 2004

한약재로 사용되는 방풍류는 절단되어 유통되므로 외부 형태적인 특징만으로 구분하기가 어려워 방풍류로 사용되는 방풍, 식방풍, 석방풍, 갯방풍 등 4종에 대해 PCR에 기초한 RAPD 마커를 이용하여 SCAR 마커를 개발하고자 하였다. RAPD 분석결과 밴드의 패턴은 다양하게 나타났으며 다형성의 밴드 수는 총 215개로 전체 밴드수의 98%였다. RAPD 분석에서 각 방풍류를 구별 할 수 있는 특이적인 밴드를 나타내는 primer는 방풍에서 4개의 primer, 식방풍은 6개의 primer, 석방풍은 4개의 primer, 갯방풍은 6개의 primer를 선발하였고, 그 중 특히 primer 425는 4종의 방풍류의 감별에 유용하였고, 이를 이용하여 SCAR마커로 전환하는데 이용하였다. 특이적인 단편을 클로닝하여 염기서열 분석으로 특이 primer를 제작하고 제작된 primer로 방풍류 시료 16개에 적용하였을 때, 국내의 야생에서 주로 자생하는 석방풍은 215 bp, 그리고 국내에서 가장 많이 재배 또는 생산되는 갯방풍은 177 bp와 300 bp에서 뚜렷하게 나타났다. 따라서 갯방풍과 석방풍의 감별 가능성을 제시할 수 있으며 개발된 SCAR 마커를 이용하여 시중에 유통되고 있는 방풍류 건조약재의 감별에 유용한 마커로 활용될 수 있을 것이다.