Discrimination and Genetic Diversity of Acanthopanax senticosus Using RAPD Markers

RAPD마커를 이용한 가시오갈피의 기원판별 및 유전적 다양성

  • 허만규 (동의대학교 자연과학대학 분자생물학과) ;
  • 최영현 (동의대학교 한의과대학 한의학과) ;
  • 최병태 (동의대학교 한의과대학 한의학과) ;
  • 김경철 (동의대학교 한의과대학 한의학과)
  • Published : 2005.08.01

Abstract

Acanthopanx senticosus (Araliaceae) is a long-lived woody species primarily distributed throughout East Asia. This species is regarded as medically and ecologically important woody plants in Korea. To identify the variation of the RAPD patterns between domestic and foreign A. senticosus species, 22 random primers were applied to Korean A. senticosus and A. senticosus for. inermis, Chinese and Russian A. senticosus. Six primers of them could be used to discriminate the origins and 58 polymorphisms among 92 scored DNA fragments. Six bands are specific for Korean A. senticosus and A. senticosus for. inermis. Especially, three primers, OPD04, OPD11 and OPE10, were useful to differentiate between domestic and foreign Acanthopanax species. RAPD analysis was a useful method to discriminate among A. senticosus populations or accessions and Korean accessions are distinct genetically.

Keywords

References

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