• Asian-Australasian Journal of Animal Sciences
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• v.32 no.7
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• pp.939-948
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• 2019

Objective: Extension and Agouti loci play a key role for proportions of eumelanin and pheomelanin in determining coat color in several species, including goat. Mongolian goats exhibit diverse types of coat color phenotypes. In this study, investigation of the melanocortin 1 receptor (MC1R) coding region in different coat colors in Mongolian goats was performed to ascertain the presence of the extension allele. Methods: A total of 105 goat samples representing three goat breeds were collected for this study from middle Mongolia. A 938 base pair (bp) long coding region of the MC1R gene was sequenced for three different breeds with different coat colors (Gobi Gurwan Saikhan: complete black, Zalaa Jinstiin Tsagaan: complete white, Mongolian native goat: admixture of different of coat colors). The genotypes of these goats were obtained from analyzing and comparing the sequencing results. Results: A total of seven haplotypes defined by five substitution were identified. The five single nucleotide polymorphisms included two synonymous mutations (c.183C>T and c.489G>A) and three missense (non-synonymous) mutations (c.676A>G, c.748T>G, and c.770T>A). Comparison of genotypes frequencies of two common missense mutions using chi-sqaure ($x^2$) test revealed significant differences between coat color groups (p<0.001). A logistic regression analysis additionally suggested highly significant association between genotypes and variation of black versus white uniform combination. Alternatively, most investigated goats (60.4%) belonged to H2 (TGAGT) haplotype. Conclusion: According to the findings obtained in this study on the investigated coat colors, mutations in MC1R gene may have the crucial role for determining eumelanin and pheomelanin phenotypes. Due to the complication of coat color phenotype, more detailed investigation needed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.6
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• pp.640-646
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• 2018

To determine whether there are differences in the food component characteristics of the cultured recessive small-sized abalone Haliotis discus hannai (SA; 30-40 each/kg) and middle-sized abalone (MA; 10-15 each/kg), the proximate compositions, fatty acid and total amino acid compositions of these two species were evaluated. Additionally, extraction methods were performed on the SA to asses the quality characteristics of the resulting. In terms of proximate composition, MA had lower moisture and carbohydrate levels and higher crude protein contents than SA. The total amino acid contents of MA and SA were 15,734.4 and 11,379.1 mg/100 g, respectively, the major amino acids were glutamic acid, aspartic acid, serine, glycine, alanine, leucine, arginine and lysine, and the major fatty acids were 16:0, 18:0, 18:1n-9, 18:1n-7, 20:4n-6, 20:5n-3, and 22:5n-3. The pH levels and total nitrogen and amino nitrogen contents of the hot-water extract (WE) and scrap enzyme hydrolysate (SE) samples from the SA were 6.32 and 6.05, 1.36% and 1.52%, and 342.1 and 403.1 mg/100 g, respectively. The extraction yields and free amino acid contents from SA were 1,317 and 440 mL/kg, and 8,721.1 and 9,070.7 mg/100 g, respectively, and the concentrations of major components were as follows: arginine, glycine, glutamic acid, alanine and lysine. Additionally, the complex extract (WE+SE) was superior to the traditional extract (WE) in terms of extraction yield, amino-nitrogen content, and organoleptic qualities but not odor.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.5
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• pp.808-813
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• 2021

The diet composition of Spanish mackerel Scomberomorus niphonius, was investigated using 853 specimens collected using two-boat trawl, stow net, gill net and set net from January to December 2019 from the South Sea of Korea. The size of the specimens ranged from 25.2 to 114.8 cm in fork length. S. niphonius fed mostly on fishes. Its diets also included small quantities of shrimps, cephalopods, crabs, stomatopods, etc. Among them, Engraulis japonicus was the dominant species. The dietary composition of S. niphonius exhibited significant differences based on their size. The proportion of Trichiurus japonicus increased as body size of S. niphonius increased, whereas the proportion of E. japonicus decreased gradually. As the body size of S. niphonius increased, the mean weight of prey per the stomach (mW/ST) tended to increase significantly (one-way ANOVA, P<0.05).

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.58-58
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• 2019

본 연구에서는 자생 참나무속 2종의 몇 가지 저광량 조건하에서 생육 및 엽록소 형광 반응을 조사하였다. 연구의 재료는 참가시나무(Quercus salicina Blume)와 붉가시나무(Q. acuta Thunb.)의 2년생 실생묘를 사용하였다. 다양한 실내 광환경조건을 조사하여 광량을 10, 50, 100 및 200 PPFD (${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$)로 설정하였으며, 실험기간 동안 광주기(12/12 h), 온도($25{\pm}1^{\circ}C$) 및 습도($55{\pm}3%$)를 동일하게 설정하여 총 8주간 재배하였다. 대조구는 유리온실에서 동일기간 동안 재배된 식물을 사용하였다. 연구의 결과, 참가시나무는 50 PPFD 이하에서 전체 식물 중 50%가 잎이 마르며 떨어지는 경향을 보였으며, 100 PPFD에서도 25%의 잎마름이 관찰되었다. 200 PPFD는 줄기직경을 제외한 초장, 엽수, 엽장, 엽폭 및 엽록소 함량(SPAD)이 대조구와 유사하거나 높았으며, 엽록소 형광 반응에서도 모든 측정값이 대조구와 유의한 차이를 보이지 않았다. 붉가시나무는 50 PPFD 이상의 광량에서는 줄기직경, 엽수, 엽장 및 SPAD가 대조구와 유사하거나 높은 결과를 보였으나, 모든 광량조건에서 초장과 엽폭이 대조구에 비해 유의적으로 낮았다. 엽록소 형광 반응은 모든 광량에서 대조구와 비슷한 양상을 보였으나, 10 PPFD는 초기형광값(Fo)과 비광학적 에너지의 손실(DIo/RC)이 대조구에 비해 유의적으로 낮은 결과를 보였다.

• Journal of Microbiology and Biotechnology
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• v.31 no.5
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• pp.686-695
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• 2021

Tricholoma matsutake is an ectomycorrhizal fungus that has a symbiotic relationship with the root of Pinus densiflora. Soil microbial communities greatly affect the growth of T. matsutake, however, few studies have examined the characteristics of these communities. In the present study, we analyzed soil fungal communities from Gyeongju and Yeongdeok using metagenomic pyrosequencing to investigate differences in fungal species diversity, richness, and taxonomic composition between the soil under T. matsutake fruiting bodies (Sample 2) and soil where the fairy ring of T. matsutake was no longer present (Sample 1). The same spot was investigated three times at intervals of four months to observe changes in the community. In the samples from Yeongdeok, the number of valid reads was lower than that at Gyeongju. The operational taxonomic units of most Sample 2 groups were less than those of Sample 1 groups, indicating that fungal diversity was low in the T. matsutake-dominant soil. The soil under the T. matsutake fruiting bodies was dominated by more than 51% T. matsutake. From fall to the following spring, the ratio of T. matsutake decreased. Basidiomycota was the dominant phylum in most samples. G-F1-2, G-F2-2, and Y-F1-2 had the genera Tricholoma, Umbelopsis, Oidiodendron, Sagenomella, Cladophialophora, and Phialocephala in common. G-F1-1, G-F2-1, and Y-F1-1 had 10 genera including Umbelopsis and Sagenomella in common. From fall to the following spring, the amount of phyla Basidiomycota and Mucoromycota gradually decreased but that of phylum Ascomycota increased. We suggest that the genus Umbelopsis is positively related to T. matsutake.

• Journal of Animal Science and Technology
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• v.63 no.2
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• pp.305-318
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• 2021

Alfalfa (Medicago sativa L.) is an important forage legume grown in Kansas, USA and its productivity with cool-season grasses however is unknown. The objective of this study was to determine the dry matter yield (DMY) and forage nutritive value of alfalfa-grass mixtures compared to those of alfalfa and grasses grown in monoculture with and without nitrogen fertilization. Three different alfalfa varieties were planted (reduced-lignin alfalfa, Roundup Ready, and conventional alfalfa) and two kinds of cool-season grasses (smooth brome, Bromus inermis Leyss, and tall fescue, Festuca arundinacea Schreb) were planted as a monoculture or in alfalfa-grass mixtures. Nitrogen fertilizer (urea) was applied at green-up at a rate of 56 kg/ha and after the second cutting at a rate of 56 kg/ha in 2016 and 2017, respectively. and control treatments received no nitrogen. DMY was significantly higher in monoculture alfalfa and alfalfa-grass mixtures than in grass monocultures. Between alfalfa monoculture and alfalfa-grass mixtures, no significant differences in DMY were found. For all treatments, nitrogen application significantly increased DMY compared to the control. In 2016 and 2017, the low-lignin alfalfa monoculture had the lowest acid detergent fiber (ADF) and the grass monocultures had the highest ADF. In 2016 and 2017, neutral detergent fiber (NDF) in smooth bromegrass and tall fescue was higher than in other species treatments. A low-lignin alfalfa monoculture had significantly lower NDF concentration compared to alfalfa-grass mixtures. When averaged over 2016 and 2017, relative feed value (RFV) was highest in low-lignin alfalfa and lowest in the grass monocultures. In both years, nitrogen fertilizer application did not affect nutritive values.

• Parasites, Hosts and Diseases
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• v.59 no.1
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• pp.103-108
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• 2021

To date, there is no report on the genetic diversity of ticks in these regions. A total of 370 representative ticks from the south and east regions of Kazakhstan (SERK) and Xinjiang Uygur Autonomous Region (XUAR) were selected for molecular comparison. A fragment of the mitochondrial cytochrome c oxidase subunit I (cox1) gene, ranging from 631 bp to 889 bp, was used to analyze genetic diversity among these ticks. Phylogenetic analyses indicated 7 tick species including Hyalomma asiaticum, Hyalomma detritum, Hyalomma anatolicum, Dermacentor marginatus, Rhipicephalus sanguineus, Rhipicephalus turanicus and Haemaphysalis erinacei from the SERK clustered together with conspecific ticks from the XUAR. The network diagram of haplotypes showed that i) Hy. asiaticum from Almaty and Kyzylorda Oblasts together with that from Yuli County of XUAR constituted haplogroup H-2, and the lineage from Chimkent City of South Kazakhstan was newly evolved; and ii) the R. turanicus ticks sampled in Israel, Almaty, South Kazakhstan, Usu City, Ulugqat and Baicheng Counties of XUAR were derivated from an old lineage in Alataw City of XUAR. These findings indicate that: i) Hy. asiaticum, R. turanicus and Ha. erinacei shared genetic similarities between the SERK and XUAR; and ii) Hy. marginatum and D. reticulatus show differences in their evolution.

• Journal of Environmental Impact Assessment
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• v.30 no.1
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• pp.24-34
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• 2021

Many countries have established and implemented marine ecosystem management plans to solve various problems arising from excessive development and use of marine and coastal areas. In this study, network analysis was applied to compare and understand marine ecosystem management's direction and characteristics in Korea and other maritime countries. The results showed that the words 'strengthen,' 'promote,' 'improve,' and 'establish' were the keywords used a lot in domestic and foreign marine ecosystem management plans. Establishing a foundation for marine ecosystem management, establishing international cooperation and partnerships, and strengthening climate change adaptation was commonly included. However, there were some differences in detailed management plans. In foreign countries, it aims to present management measures for certain species and improve the existing institutional foundation. Still, in Korea, it aims to strengthen the comprehensive management of marine life and establish an institutional foundation for marine ecosystems. This study is expected to help understand the direction of domestic and overseas marine ecosystem management and establish a domestic marine ecosystem management plan in the future.

• Journal of Microbiology and Biotechnology
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• v.31 no.12
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• pp.1709-1715
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• 2021

Outbreaks of food poisoning due to the consumption of norovirus-contaminated shellfish continue to occur. Male-specific (F+) coliphage has been suggested as an indicator of viral species due to the association with animal and human wastes. Here, we compared two methods, the double agar overlay and the quantitative real-time PCR (RT-PCR)-based method, for evaluating the applicability of F+ coliphage-based detection technique in microbial contamination tracking of shellfish samples. The RT-PCR-based method showed 1.6-39 times higher coliphage PFU values from spiked shellfish samples, in relation to the double agar overlay method. These differences indicated that the RT-PCR-based technique can detect both intact viruses and non-particle-protected viral DNA/RNA, suggesting that the RT-PCR based method could be a more efficient tool for tracking microbial contamination in shellfish. However, the virome information on F+ coliphage-contaminated oyster samples revealed that the high specificity of the RT-PCR- based method has a limitation in microbial contamination tracking due to the genomic diversity of F+ coliphages. Further research on the development of appropriate primer sets for microbial contamination tracking is therefore necessary. This study provides preliminary insight that should be examined in the search for suitable microbial contamination tracking methods to control the sanitation of shellfish and related seawater.

• Journal of Cadastre & Land InformatiX
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• v.52 no.1
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• pp.95-104
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• 2022

This study aims to find the exact area of the forest fire in Okgye-myeon, Gangneung, April 4, 2019. Since there is a gradient in our country's forests, we should find a surface area that takes into account The 5th numerical clinical map provided by the DEM and the Korea Forest Service provided by the National Geographic Information Service was used. In DEM, the center point of each pixel was created and all points were connected. The length of the connecting line is determined by the spatial resolution of the pixel and the cosine value, and the surface area is obtained along with the height value, which is called the Slope-Aspect algorithm. The surface area and floor area of the forest were shown according to the tree species and types of forest, and their quantitative numerical differences proved the validity of this study.