• 대한원격탐사학회지
• /
• 제33권2호
• /
• pp.213-230
• /
• 2017

위성기반 적조 탐지 알고리즘들은 특정 해역, 적조 종을 중심으로 개발되어 왔다. 하지만 한반도 주변해역의 빠르고 정확한 적조 감시를 위해서는 한반도 주변의 전 발생 해역과 다양한 적조 종을 대상으로 한 연구가 필수적이다. 본 연구에서는 한반도 주변해역을 대상으로 GOCI 영상을 활용하여 적조 영역의 스펙트럼 특성과 위성기반 적조 탐지 알고리즘의 적합성을 분석하였다. 그 결과, 적조 종들의 클로로필 함량과 적조가 출현하는 해역의 탁도에 따라서 스펙트럼 특성이 달라졌다. 또한 기존 적조 탐지 알고리즘을 GOCI 영상에 적용하였으며, 이를 통해 기존 임계값으로는 적조 영역 추출에 한계가 있음을 알 수 있었다. 이를 개선하기 위해 적조 종들을 클로로필 함량의 차이에 따라 두 그룹으로 나누어 적조 감시 체계를 제시하였다. 총 5 단계를 거쳐 적조 영역과 비적조 영역을 구분하였으며, 적조 속보를 기준으로 했을 경우 최종 추출된 적조영역이 기존 알고리즘으로 추출된 영역에 비해 적절한 결과를 나타냈다. 이러한 적조 감시 체계를 활용한다면 한반도 주변의 모든 해역과 다양한 적조 종에 대한 빠르고 정확한 감시로 인해 효율적인 적조 감시가 가능할 것이다.

• 한국동물위생학회지
• /
• 제35권3호
• /
• pp.215-222
• /
• 2012

Species-specific $TaqMan^{(R)}$ probe-based real-time PCR assays were developed for detection of beef, pork, chicken, duck, goose and turkey. The primer and probe sets used in this study were designed to be complementary to fibroblast growth factor (FGF) for cattle and pig, mitochondrial NADH dehydrogenase (ND) subunit 3 and ND2 for chicken and duck, 12S rRNA for goose and turkey, respectively. As internal positive control we used conserved region in the ribosomal 18S RNA gene to ensure the accuracy of the detection of target DNA by real-time PCR. We confirmed that real-time PCR assays with the primer and probe sets were positive for cattle, pig and chicken intended target animal species with no cross-reactivity with other non-target animal species. Only >50 ng DNA of beef show cross-reactivity in the determination of duck. Using species-specific primer and probe sets, it was possible to detect amounts of 0.1 ng DNA of cattle and pig, 1.0 pg DNA of chicken, duck and turkey, and 0.1 pg DNA of goose for raw samples, respectively. The detection limits were 0.1 ng DNA of cattle, 1.0 ng DNA of pig and 1.0 pg DNA of chicken for DNA mixtures (beef, pork and chicken) extracted from heat-treated ($121^{\circ}C$/5 min) meat samples. In conclusion, it can be suggested that the $TaqMan^{(R)}$ probe-based assay developed in this study might be a rapid and specific method for the identification of meat species in raw or cooked meat products.

• 산업기술연구
• /
• 제28권A호
• /
• pp.141-147
• /
• 2008

Several contaminated bacteria such as Lactobacillus brevis and Pediococcus damnosus in beer production cause beer spoilage by producing off flavours and turbidity. Detection of these organisms is complicated by the strict anaerobic conditions and lengthy incubation times required for their cultivation, consequently there is a need for more rapid detection methods. Recently, two contaminated strains were isolated from vessel of beer production and identified as Lactobacillus species by API kit identificaton as well as 16S-23S ITS sequencing analyses. Two isolated strains were named as Lactobacillus sp. HLA1 and Lactobacillus HLB2, respectively. A polymerase chain reaction (PCR) method was developed for the rapid and specific detection of Lactobacillus sp.. Two sets of primer pairs (HLA1-F/HLA1-R and HLB2-F/HLB2-R) were designed for the amplification of a 1576 base pair (bp) fragment of the HLA1 16S-23S rRNA gene and 1888 bp fragement of the HLB2 16S-23S rRNA. Amplified PCR products were highly specific to detect corresponding bacteria when other contaminated strains were used as PCR templates. However, detection of both strains were limited when $100{\mu}{\ell}$ of cultured samples were mixed with $100m{\ell}$ of beer sample in arbitrary manner. The sensitivity of the assay still needs to be improved for direct detection of the small amounts of bacteria present in beer.

• Parasites, Hosts and Diseases
• /
• 제54권4호
• /
• pp.503-507
• /
• 2016

The genus Spirometra belongs to the family Diphyllobothriidae and order Pseudophyllidea, and includes intestinal parasites of cats and dogs. In this study, a plerocercoid labeled as Spirometra mansonoides from the USA was examined for species identification and phylogenetic analysis using 2 complete mitochondrial genes, cytochrome c oxidase I (cox1) and NADH dehydrogenase subunit 3 (nad3). The cox1 sequences (1,566 bp) of the plerocercoid specimen (USA) showed 99.2% similarity to the reference sequences of the plerocercoid of Korean Spirometra decipiens (GenBank no. KJ599679), and 99.1% similarity in regard to nad3 (346 bp). Phylogenetic tree topologies generated using 4 analytical methods were identical and showed high confidence levels with bootstrap values of 1.00, 100%, 100%, and 100% for Bayesian inference (BI), maximum-likelihood (ML), neighbor-joining (NJ), and maximum parsimony (MP) methods, respectively. Representatives of Diphyllobothrium and Spirometra species formed a monophyletic group, and the sister-genera status between these species was well supported. Trapezoic proglottids in the posterior 1/5 region of an adult worm obtained from an experimentally infected cat were morphologically examined. The outer uterine loop of the uterus coiling characteristically consisted of 2 complete turns. The results clearly indicated that the examined Spirometra specimen from the USA matched to S. decipiens very well, and indicated possible presence of the life cycle of this species in this region.

• 한국축산식품학회지
• /
• 제36권4호
• /
• pp.463-468
• /
• 2016

Contamination by foodborne pathogens and mycotoxins was examined in 475 eggs and 20 feed samples collected from three egg layer farms, three egg-processing units, and five retail markets in Korea. Microbial contamination with Salmonella species, Escherichia coli, and Arcobacter species was examined by bacterial culture and multiplex polymerase chain reaction (PCR). The contamination levels of aflatoxins, ochratoxins, and zearalenone in eggs and chicken feeds were simultaneously analyzed with high-performance liquid chromatography coupled with fluorescence detection after the post-derivatization. While E. coli was isolated from 9.1% of eggs, Salmonella species were not isolated. Arcobacter species were detected in 0.8% of eggs collected from egg layers by PCR only. While aflatoxins, ochratoxins, and zearalenone were found in 100%, 100%, and 85% of chicken feeds, their contamination levels were below the maximum acceptable levels (1.86, 2.24, and 147.53 μg/kg, respectively). However, no eggs were contaminated with aflatoxins, ochratoxins, or zearalenone. Therefore, the risk of contamination by mycotoxins and microbes in eggs and chicken feeds is considered negligible and unlikely to pose a threat to human health.

• 농업과학연구
• /
• 제40권4호
• /
• pp.281-287
• /
• 2013

우리나라의 격리재배는 102속, 약 250여종의 식물에 대해 실시하고 있으며, 직접경검법, 배양법, 선택배지, 생리생화학, ELISA 및 PCR 검사방법을 사용한다. 2005-2012년, 우리나라에서 수행된 격리재배는 총 8,307건이며 이중 구근류가 5,165건(62.2%)로 가장 많았고, 묘목류가2,119건(25.0%), 종자 796건(9.6%), 삽수 150건(1.8%), 접수 70건(0.8%) 및 기타 7건(0.1%) 이었다. 불합격 사례는 총 413건으로 약 4.97%였고, 발견된 병의 종류는 총 47종으로 나타났다. 종류별로는 바이러스가 27종으로 가장 많은 수를 차지했으며, 곰팡이 16종, 바이로이드 1종, Chromalveolata 1종 및 기타 2종으로 나타났다. 가장 많은 검역건을 올린 병원체는 Arabis mosaic virus (77건), Tobacco rattle virus (70건), Lily symptomless virus (46건), Penicillium expansum (46건)이다.

• The Plant Pathology Journal
• /
• 제29권4호
• /
• pp.357-363
• /
• 2013

The fungus Venturia nashicola is the causal agent of scab on Asian pears. For the rapid and reliable identification as well as sensitive detection of V. nashicola, a PCR-based technique was developed. DNA fingerprints of three closely related species, V. nashicola, V. pirina, and V. inaequalis, were obtained by random amplified polymorphic DNA (RAPD) analysis. Two RAPD markers specific to V. nashicola were identified by PCR, after which two pairs of sequence characterized amplified region (SCAR) primers were designed from the nucleotide sequences of the markers. The SCAR primer pairs, designated as D12F/D12R and E11F/E11R, amplified 535-bp and 525-bp DNA fragments, respectively, only from genomic DNA of V. nashicola. The specificity of the primer sets was tested on strains representing three species of Venturia and 20 fungal plant pathogens. The nested PCR primer pair specific to V. nashicola was developed based on the sequence of the species-specific 525-bp DNA fragment amplified by primer set E11F/E11R. The internal primer pair Na11F/Na11R amplified a 235-bp fragment from V. nashicola, but not from any other fungal species tested. The nested PCR assay was sensitive enough to detect the specific fragment in 50 fg of V. nashicola DNA.

• 미생물학회지
• /
• 제48권4호
• /
• pp.229-239
• /
• 2012

Cronobacter spp. (formerly Enterobacter sakazakii), a Gram-negative bacillus, is a rare cause of meningitis and central nervous system infections. In England, the first case infected by this organism occurred in 1958. By July 2008, approximately 120 documented cases of Cronobacter spp. infection and at least 27 deaths have been identified from all around the world in the published literature and in reports submitted by public health sectors. In 2007, it was proposed by European organizations that the original taxonomy of E. sakazakii would be revised, to consist of five new species moved to a new genus, and identified as "Cronobacter". E. sakazakii has thus now been reclassified as 6 separate species in the new genus, Cronobacter, gen. nov., within the Enterobacteriaceae family. The new species are presently Cronobacter sakazakii, C. turicensis, C. malonaticus, C. muytjensii, and C. dublinensis; the sixth species is identified simply as genomospecies I, as currently including only two representative strains. The objectives of this review are to provide insight on (1) the classification and taxonomy of Cronobacter spp., (2) its clinical etiology and pathogenicity, (3) prequency of Cronobacter spp. in different categories of ready-to-eat food other than infant formula, (4) methods for detecting, isolating and typing Cronobacter spp., and (5) recent research trends for detecting Cronobacter spp.

• 전기전자학회논문지
• /
• 제24권4호
• /
• pp.1141-1147
• /
• 2020

말벌 종은 모양이 매우 유사하기 때문에 비전문가가 분류하기 어렵고, 객체의 크기가 작고 빠르게 움직이기 때문에 실시간으로 탐지하여 종을 분류하는 것은 더욱 어렵다. 본 논문에서는 바운딩 박스를 이용한 딥러닝 알고리즘을 기반으로 말벌 종을 실시간으로 분류하는 시스템을 개발하였다. 훈련 영상의 레이블링 작업 시 바운딩 박스 안에 포함되는 배경 영역을 최소화하기 위하여 말벌의 머리와 몸통 부분만을 선택하는 방법을 제안한다. 또한 실시간으로 말벌을 탐지하고 그 종을 분류할 수 있는 최선의 알고리즘을 찾기 위하여 기존의 바운딩 박스 기반 객체 인식 알고리즘들을 실험을 통하여 비교한다. 실험 결과 컨볼루션 레이어의 활성함수로 mish 함수를 적용하고, 객체 검출 블록 전에 공간집중모듈(Spatial Attention Module, SAM)을 적용한 YOLOv4 모델을 사용하여 말벌 영상을 테스트한 경우 평균 97.89%의 정밀도(Precision)와 98.69%의 재현율(Recall)을 나타내었다.

• 대한본초학회지
• /
• 제39권2호
• /
• pp.1-9
• /
• 2024

Objectives : Paeng-hwal is described as an insect herbal medicine used for digestive diseases in the Dong-ui-bo-gam. The origin of this herbal medicine is limited to several small crabs, such as Helice tridens. These crab species cohabitat in the same environment and share similar morphological characteristics, making it very difficult to distinguish and collect the individual species for use in dietary supplements or herbal medicines. This study was conducted to develop a genetic identification tool for discriminating among these closely related small crab species. Methods : CO1 DNA barcode regions of 15 samples from 6 species of small crabs were analyzed to obtain the individual sequences. To identify the correct species, comparative analyses were carried out using the database of the NCBI GenBank and the NIBR. SCAR primers were designed to develop simple and rapid assay methods using inter-species specific sequences. Optimal SCAR assay conditions were established through gradient PCR, and the limit of detection (LOD) was determined. Results : Six species of small crabs (Helicana tridens, Macrophthalmus abbreviatus, Helicana tientsinensis, Helicana wuana, Chiromantes dehaani, and Hemigrapsus penicillatus), which are distributed as Paeng-hwal, were identified through CO1 sequences analysis. We also developed SCAR markers to distinguish between six small crabs at the species level. Furthermore, we established the optimal PCR assay methods and the LOD of each individual species. Conclusions : The rapid and simple SCAR-PCR assay methods were developed to identify the species and control the quality of herbal medicines for Paeng-hwal based on the genetic analyses of CO1 DNA barcodes.