Specific and Sensitive Detection of Venturia nashicola, the Scab Fungus of Asian Pears, by Nested PCR |
Koh, Hyun Seok
(Department of Biology, Sunchon National University)
Sohn, San Ho (Department of Biology, Sunchon National University) Lee, Young Sun (Department of Biology, Sunchon National University) Koh, Young Jin (Department of Plant Medicine, Sunchon National University) Song, Jang Hoon (Pear Research Station, National Institute of Horticultural & Herbal Science) Jung, Jae Sung (Department of Biology, Sunchon National University) |
1 | Asano, T., Senda, M., Suga, H. and Kageyama, K. 2010. Development of multiplex PCR to detect five Pythium species related to turfgrass disease. J. Phytopathol. 158:609-615. |
2 | Beck, A., Ritschel, A., Schubert, K., Braun, U. and Triebel, D. 2005. Phylogenetic relationships of the anamorphic genus Fusicladium s. lat. as inferred by ITS nrDNA data. Mycol. Prog. 4:111-116. DOI |
3 | Chen, L. S., Chu, C., Liu, C. D., Chen, R.S. and Tsay, J. G. 2006. PCR-based detection and differentiation of anthracnose pathogens, Colletotrichum gloeosporioides and C. truncatum, from vegetable soybean in Taiwan. J. Phytopathol. 154:654-662. DOI ScienceOn |
4 | Chioccetti, A., Sciaudone, L., Durando, F., Garibaldi, A. and Migheli, Q. 2001. PCR detection of Fusarium oxysporum f. sp. basilici on basil. Plant Dis. 85:607-611. DOI ScienceOn |
5 | Grosch, R., Schneider, J. H. M., Peth, A., Waschke, A., Franken, P., Kofoet, A. and Jabaji-Hare, S. H. 2006. Development of a specific PCR assay for the detection of Rhizoctonia solani AG 1-IB using SCAR primers. J. Appl. Microbiol. 102:806-819. |
6 | Grote, D., Olmos, A., Kofoet, A., Tuset, J. J., Bertolini, E. and Cambra, M. 2002. Specific and sensitive detection of Phytophthora nicotianae by simple and nested-PCR. Eur. J. Plant Pathol. 108:197-207. DOI ScienceOn |
7 | Ishii, H. and Yanase, H. 2000. Venturia nashicola, the scab fungus of Japanese and Chinese pears: a species distinction from V. pirina. Mycol. Res. 104:755-759. DOI ScienceOn |
8 | Le Cam, B., Devaux, M. and Parisi, L. 2001. Specific polymerase chain reaction identification of Venturia nashicola using internally transcribed spacer region in the ribosomal DNA. Phytopathology 91:900-904. DOI ScienceOn |
9 | Li, H. B., Wu, X. Q., Peng, H. Z., Fu, L. Z., Wei, H. L., Wu, Q. Q., Jin, Q. Y. and Li, N. 2008. New available SCAR markers;potentially useful in distinguishing a commercial strain of the superior type from other strains of Lentinula edodes in China. Appl. Micribiol. Biotechnol. 81:303-309. DOI |
10 | Lopez-Mondejar, R., Beaulieu, R., Ros, M. and Pascual, A. 2012. SCAR-based real-time TaqMan PCR for early detection of Fusarium oxysporum in melon seedlings under greenhouse nursery conditions. Crop Prot. 33:1-6. DOI ScienceOn |
11 | Ma, Z. and Michailides, T. J. 2002. A PCR-based technique for identification of Fusicoccum sp. from pistachio and various other hosts in California. Plant Dis. 86:515-520. DOI ScienceOn |
12 | Martin, R. R., James, D. and Levesque, C. A. 2000. Impacts of molecular diagnostic technologies on plant disease management. Annu. Rev. Phytopathol. 38:207-239. DOI ScienceOn |
13 | Sambrook, J. and Russell, D. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, New York, USA. 6.33-6.43 pp. |
14 | Ni, H. F., Yang, H. R., Chen, R. S., Hung, T. H. and Liou, R. F. 2012. A nested multiplex PCR for species-specific identification and detection of Botryosphaeriaceae species on mango. Eur. J. Plant Pathol. 133:819-828. DOI |
15 |
Rigotti, S., Gindro, K., Richter, H. and Viret, O. 2002. Characterization of molecular markers for specific and sensitive detection of Botrytis cinerea Pers.: Fr in strawberry (Fragaria |
16 | Rozen, S. and Skaletsky, H. J. 2000. Primer 3 on the WWW for general users and for biologist programmers, In: Bioinformatics Methods and Protocols: Methods in Molecular Biology, ed. by S. Krawetz and S. Misener, pp. 365-386. Humana Press, Totowa, USA. |
17 | Schnabel, G., Schnabel, E. L. and Jones, A. L. 1999. Characterization of ribosomal DNA from Venturia inaequalis and its phylogenetic relationship to rDNA from other tree-fruit Venturia species. Phytopathology 89:100-108. DOI ScienceOn |
18 | Shabi, E. 1990. Pear scab, In: Compendium of Apple and Pear Diseases, ed. by A. L. Jones and H. S. Aldwinckle, pp 22-23. APS Press, St. Paul, USA. |
19 | Shabi, E., Roten, J. and Loebenstein, G. 1973. Physiological races of Venturia pirina on pear. Phytopathology 63:41-43. DOI |
20 | Tanaka, S. and Yamamoto, S. 1964. Studies on pear scab. II. Taxonomy of the causal fungus of Japanese pear scab. Ann. Phytopathol. Soc. Jpn. 29:128-136. DOI |
21 | Vandemark, G. J., Kraft, J. M., Larsen, R. C., Gritsenko, M. A. and Boge, W. L. 2000. A PCR-based assay by sequence-characterized DNA markers for the identification and detection of Aphanomyces euteiches. Phytopathology 90:1137-1144. DOI ScienceOn |
22 | Zhao, P., Kakishima, M., Uzuhashi, S. and Ishii, H. 2012. Multigene phylogenetic analysis of inter- and intraspecific relationships in Venturia nashicola and V. pirina. Eur. J. Plant Pathol. 132:245-258. DOI |
23 | White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, In: PCR Protocol: A Guide to Methods and Applications, ed. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp 315-322. Academic Press, New York, USA. |
24 | Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A. and Tingey, S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18:6531-6535. DOI ScienceOn |
![]() |