• The Korean Journal of Mycology
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• v.40 no.3
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• pp.141-144
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• 2012

Total 50 species, 80 strains: Eighty strains of yeasts were isolated from wild flowers of Daejeon city and its surroundings in Korea. Isolated yeasts were identified by comparison of their PCR-amplified 18s rDNA V3 region or internal transcribed spacer-2 (ITS-2) genes with BLAST database. Among them, 32 species, 54 strains of yeasts were isolated and identified from wild flowers of Daejeon city, and Cryptococcus species were dominant yeasts. Furthermore, 18 species, 26 strains of yeasts were isolated and identified from wild flowers of Geumsan-gun and Nonsan-gun, Chungcheongnam-do in Korea, and also Candida species and Pseudozyma species were dominant yeasts.

• Journal of Microbiology and Biotechnology
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• v.20 no.12
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• pp.1637-1646
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• 2010

The bacterium Deinococcus radiodurans is one of the most resistant organisms to ionizing radiation and other DNA-damaging agents. Although, at present, 30 Deinococcus species have been identified, the whole-genome sequences of most species remain unknown, with the exception of D. radiodurans (DRD), D. geothermalis, and D. deserti. In this study, comparative genomic hybridization (CGH) microarray analysis of three Deinococcus species, D. radiopugnans (DRP), D. proteolyticus (DPL), and D. radiophilus (DRPH), was performed using oligonucleotide arrays based on DRD. Approximately 28%, 14%, and 15% of 3,128 open reading frames (ORFs) of DRD were absent in the genomes of DRP, DPL, and DRPH, respectively. In addition, 162 DRD ORFs were absent in all three species. The absence of 17 randomly selected ORFs was confirmed by a Southern blot. Functional classification showed that the absent genes spanned a variety of functional categories: some genes involved in amino acid biosynthesis, cell envelope, cellular processes, central intermediary metabolism, and DNA metabolism were not present in any of the three deinococcal species tested. Finally, comparative genomic data showed that 120 genes were Deinococcus-specific, not the 230 reported previously. Specifically, ddrD, ddrO, and ddrH genes, previously identified as Deinococcus-specific, were not present in DRP, DPL, or DRPH, suggesting that only a portion of ddr genes are shared by all members of the genus Deinococcus.

• Journal of Microbiology and Biotechnology
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• v.24 no.7
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• pp.888-897
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• 2014

A bacterial community analysis of the gut of Tenebrio molitor larvae was performed using pyrosequencing of the 16S rRNA gene. A predominance of genus Spiroplasma species in phylum Tenericutes was observed in the gut samples, but there was variation found in the community composition between T. molitor individuals. The gut bacteria community structure was not significantly affected by the presence of antibiotics or by the exposure of T. molitor larvae to a highly diverse soil bacteria community. A negative relationship was identified between bacterial diversity and ampicillin concentration; however, no negative relationship was identified with the addition of kanamycin. Ampicillin treatment resulted in a reduction in the bacterial community size, estimated using the 16S rRNA gene copy number. A detailed phylogenetic analysis indicated that the Spiroplasma-associated sequences originating from the T. molitor larvae were distinct from previously identified Spiroplasma type species, implying the presence of novel Spiroplasma species. Some Spiroplasma species are known to be insect pathogens; however, the T. molitor larvae did not experience any harmful effects arising from the presence of Spiroplasma species, indicating that Spiroplasma in the gut of T. molitor larvae do not act as a pathogen to the host. A comparison with the bacterial communities found in other insects (Apis and Solenopsis) showed that the Spiroplasma species found in this study were specific to T. molitor.

• Korean journal of applied entomology
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• v.46 no.3
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• pp.375-383
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• 2007

To find out the affection of the sampling techniques to the result of a faunistic study, we surveyed the insect fauna of the Chungbuk National University (four different sites) for a year, from spring to fall. For each site, four different collecting methods: light trap, net sweeping, pitfall trap, and window trap, were applied and the collecting was done every other week for a total of 16 times. A total of 14 orders and 672 species were collected. 501 species were collected by the light trap, which covers about 75% of the total number of species, turn out to be the most effective, while other methods could only cover 18% or less. On average, only about 30% of the species collected at a given time of collecting were re-collected at the next collecting, which means about 70% of the species collected from the first collecting remains not collected in the next collecting if you collect insects every other week. The result suggests that, in addition to applying diverse collecting methods, frequent sampling, or narrow sample window, is another very important factor for a good representation of species diversity in an insect faunistic study.

• Journal of Forest and Environmental Science
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• v.33 no.4
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• pp.315-321
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• 2017

This study was carried out to estimate the parameters of stem taper functions, to figure out the best taper model by species, and to compare with previous studies by species, targeting on the stemmed tree samples collected from the Korean red pine (Pinus densiflora), Korean white pine (Pinus koraiensis), and Japanese larch (Larix kaempferi ) stands in Gangwon and North Gyeongsang provinces of South Korea. The seven widely used models were applied in this study, and Muhairwe 1999 model for Korean red pine and Korean white pine and Kozak 2002 model for Japanese larch were evaluated as the best model for each species according to the fit statistics and the predicted stem form comparison. In addition, the predicted diameter was suitably fitted when comparing the previous studies, and the values were more appropriate following stem taper according to neiloid, paraboloid, and cone parts by species. Consequently, the estimation of this study was considered to represent the stem taper well. When comparing stem taper of three species, the diameter was largest in Korean white pine. Overall, the taper models of this study are judged to be useful for estimating stem form and volume computation of Korean red pine, Korean white pine, and Japanese larch.

• Parasites, Hosts and Diseases
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• v.57 no.6
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• pp.647-656
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• 2019

Isthmiophora hortensis (Digenea: Echinostomatidae) is a dominant echinostome in animal reservoir hosts and humans in the Republic of Korea (Korea). We intended to investigate the infection status with this echinostome species in the several species of wild animals and describe the morphological characteristics in the faunistic view point. A total of 175 animal carcasses belonging to 3 families, i.e., Canidae, Felidae and Mustelidae, were collected from the southern regions of Korea from March 2010 to July 2017. Isthmiophora spp. worms were recovered from the small intestines of each animal under a stereomicroscope after washing of intestinal contents. Isthmiophora hortensis was recovered from 4 species of wild carnivores, i.e., Nyctereutes procyonoides (3/107: 2.8%), Mustela sibirica (11/31: 35.5%), Meles lucurus (2/3: 33.3%) and Martes flavigula (1/2: 50%). The other 3 carnivores comprising stray dogs, cat and leopard cat were negative for I. hortensis infection (0/2, 0/10 and 0/12, respectively). Specimens obtained from the Lutra lutra (6/8: 75%) were identified as a distinct species, I. inermis, by morphological comparison. Isthmiophora inermis has thinner body, elongate testes and different anterior limits of vitelline fields. Detailed morphological descriptions and comparisons with the morphological characteristics are provided. Conclusively, it was confirmed for the first time that 3 species of mustelid mammals, i.e., M. sibirica, M. lucurus, and M. flavigula, are to be the new definitive hosts of I. hortensis in Korea. Additionally, I. inermis is to be newly added in the Korean echinostome fauna.

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.2
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• pp.65-69
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• 1990

An experiment was carried out ot determine the effects of seeding rates and seedbed preparations on the establishment and yield of surface-sown pasture species. Establishment of orchardgrass was significantly increased by the lowest seeding rate of 20 kg/ha as compared to 30 and 40 kg/ha, but no differences were found between the seeding rates of ladino clover. Both raking and burning significantly improved the establishment of pasture species in comparison with non-treatment. There was a significant difference between the raking and burning for the establishment of orchardgrass, but no significant difference was found between the two treatments for that of ladino clover. Increasing seeding rate of pasture species did not enhance the yield of surface-sown pasture, but both the raking and non-treatment significantly increased the yield of pasture species. The results suggest that both the raking and burning can improve the establishment but increasing seeding rate over 20 kg/ha and burning may not increase the yield.

• Korean Journal of Agricultural Science
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• v.46 no.3
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• pp.529-538
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• 2019

This study examined the changes in plant phenology of 12 woody species in Daegu University Forest, Gyeongsan-si, Gyeongsanbuk-do in 2007 and 2018. The Hobo was installed at a height of 1.2 meters to measure the microclimate such as air temperature and relative humidity to identify its effects on the changes in plant phenology. The number of trees surveyed were 42 individuals that included 12 species in 9 families, all of which are deciduous broad-leaved trees. The trends in temperature change in 2018 were similar to that in 2007, except for the temperatures in the warmest and coldest month. Compared to 2007, the average temperature of the warmest month in 2018 was $2.5^{\circ}C$ higher, and the coldest month was $3.3^{\circ}C$ lower. The lowest temperature from February to April in 2018 had the highest correlation with the blooming dates of the trees (r = 0.692) in 2018, and the highest temperature showed the lowest correlation (r = 0.392). The blooming date by species was significantly different (p < 0.05). The blooming date of 8 species (75%), including Prunus padus, was earlier by 1 to 16 days, while 4 species, such as Prunus armeniaca var. ansu, was later by 1 to 7 days in 2018 than that in 2007. However, the flowering duration did not have a significant effect on the plant phenology. The results can be used as basic data for long-term monitoring of plant phenology in the future, and follow-up studies on other environmental factors and physiological factors are needed.

• Biomedical Science Letters
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• v.25 no.1
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• pp.40-53
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• 2019

Of the Acinetobacter spp., A. baumannii (genospecies 2) is the most clinically significant in terms of hospital-acquired infections worldwide. It is difficult to perform Acinetobacter-related taxonomy using phenotypic characteristics and routine laboratory methods owing to clusters of closely related species. The ability to accurately identify Acinetobacter spp. is clinically important because antimicrobial susceptibility and clinical relevance differs significantly among the different genospecies. Based on the medical importance of pathogenic Acinetobacter spp., the distribution and characterization of Acinetobacter spp. isolates from 123 clinical samples was determined in the current study using four typically applied bacterial identification methods; partial rpoB gene sequencing, amplified rRNA gene restriction analysis (ARDRA) of the intergenic transcribed spacer (ITS) region of the 16~23S rRNA, the $VITEK^{(R)}$ 2 system (an automated microbial identification system) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). A. baumannii isolates (74.8%, 92/123) were the most common species, A. nosocomialis (10.6%, 13/123) and A. pittii isolates (7.5%, 9/123) were second and third most common strains of the A. calcoaceticus-A. baumannii (ACB) complex, respectively. A. soli (5.0%, 6/123) was the most common species of the non-ACB complex. RpoB gene sequencing and ARDRA of the ITS region were demonstrated to lead to more accurate species identification than the other methods of analysis used in this study. These results suggest that the use of rpoB genotyping and ARDRA of the ITS region is useful for the species-level identification of Acinetobacter isolates.

• Journal of People, Plants, and Environment
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• v.24 no.3
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• pp.267-275
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• 2021

Background and objective: Particulate matter (PM) has a fatal effect on health. There have been many studies on the use of plants such as trees and shrubs as eco-friendly and sustainable biofilter for the removal of PM. In forming more green space, ground cover plants play an important role in multi-layered planting. This study was conducted to investigate the ability of plants to reduce PM, targeting Korean native ground cover plants with high availability in urban green spaces. Methods: For 4 species of Asteraceae, 4 species of Liliaceae, and 3 species of Rosaceae, one species of plants at a time were placed in an acrylic chamber (800 × 800 × 1000 mm, L × W × H) modeling an indoor space. After the injection of PM, the amount of PM remaining in the chamber over time was investigated. Results: For all three types of PM (PM10, PM2.5, PM1), significant difference occurred in the amount of PM remaining between plant species after 1 hour in the Liliaceae chamber, 3 hours in the Asteraceae chamber, and 5 hours in the Rosaceae chamber. With Liliaceae, the leaf area and the amount of PM remaining in the chamber showed a negative (-) correlation. With the Asteraceae and Rosaceae, there was a weak negative correlation between the leaf area and the amount of PM remaining in the chamber. Conclusion: When using ground cover plants as a biofilter to remove PM, it is considered effective to select a species with a large total leaf area, especially for Liliaceae.