• Title/Summary/Keyword: Spatial Domain

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Novel splice isoforms of pig myoneurin and their diverse mRNA expression patterns

  • Guo, Xiaohong;Li, Meng;Gao, Pengfei;Cao, Guoqing;Cheng, Zhimin;Zhang, Wanfeng;Liu, Jianfeng;Liu, Xiaojun;Li, Bugao
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.10
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    • pp.1581-1590
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    • 2018
  • Objective: The aim of this study was to clone alternative splicing isoforms of pig myoneurin (MYNN), predict the structure and function of coding protein, and study temporal and spatial expression characteristics of each transcript. Methods: Alternative splice isoforms of MYNN were identified using RNA sequencing (RNA-seq) and cloning techniques. Quantitative real-time polymerase chain reaction (qPCR) was employed to detect expression patterns in 11 tissues of Large White (LW) and Mashen (MS) pigs, and to study developmental expression patterns in cerebellum (CE), stomach (ST), and longissimus dorsi (LD). Results: The results showed that MYNN had two alternatively spliced isoforms, MYNN-1 (GenBank accession number: KY470829) and MYNN-2 (GenBank accession number: KY670835). MYNN-1 coding sequence (CDS) is composed of 1,830 bp encoding 609 AA, whereas MYNN-2 CDS is composed of 1,746 bp encoding 581 AA. MYNN-2 was 84 bp less than MYNN-1 and lacked the sixth exon. MYNN-2 was found to have one $C_2H_2$ type zinc finger protein domain less than MYNN-1. Two variants were ubiquitously expressed in all pig tissues, and there were significant differences in expression of different tissues (p<0.05; p<0.01). The expression of MYNN-1 was significantly higher than that of MYNN-2 in almost tissues (p<0.05; p<0.01), which testified that MYNN-1 is the main variant. The expression of two isoforms decreased gradually with increase of age in ST and CE of MS pig, whereas increased gradually in LW pig. In LD, the expression of two isoforms increased first and then decreased with increase of age in MS pig, and decreased gradually in LW pig. Conclusion: Two transcripts of pig MYNN were successfully cloned and MYNN-1 was main variant. MYNN was highly expressed in ST, CE, and LD, and their expression was regular. We speculated that MYNN plays important roles in digestion/absorption and skeletal muscle growth, whereas the specific mechanisms require further elucidation.

Comparative Assessment of a Method for Extraction of TC-induced Rainfall Affecting the Korean Peninsula (한반도 태풍강우 추출기법 비교 평가)

  • Son, Chan-Young;Kwon, Hyun-Han;Kim, Jong-Suk;Moon, Young-Il
    • Journal of Korea Water Resources Association
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    • v.47 no.12
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    • pp.1187-1198
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    • 2014
  • Strong winds and heavy rainfall from tropical cyclones (TCs) that occur in the Northwestern Pacific cause significant human and material damage to the Korean peninsula and East Asia. Hence, it is important to establish early warning systems and conduct preparedness activities in advance of a TC. This study suggests a technique to extract the value of uniform TC-induced rainfall considering the TC track and TC size. To validate our technique, we compare it to existing TC rainfall techniques using the spatial domain. To determine the TC size required for extracting TC-induced rainfall, this research analyzed the mean of TC-induced rainfall by TC size (1973-2012). As a result of this analysis, the maximum amount of mean of TC-induced rainfall was found for a TC with a radius of 700 km. Other techniques have limitations which this new technique addresses; it can extract TC-induced rainfall in each administrative area and minimize systematic biases of other extraction methods. The result of this study can be utilized in the preparation of rainfall forecasts, designing hydraulic structures, and predicting landslide and debris flows using TC-induced rainfall and downpours.

Estimation of Flood Risk Index for the Nakdong River Watershed (낙동강 유역의 홍수위험지수 산정)

  • Song, Jae Ha;Kim, Sangdan;Park, Moo Jong;Choi, Hyun Il
    • Journal of Korea Water Resources Association
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    • v.46 no.1
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    • pp.35-45
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    • 2013
  • The aim of study is to present how to estimate and use the FRI (Flood Risk Index) for classifying area zones based on regional flooding risk in terms of the integrated flood risk management. To estimate the FRI at a spatial resolution of city/county/town units for the Nakdong River Watershed, the 17 representative flood indexing factors are carefully selected for the three flood indexes, such as PI (Pressure Index), SI (State Index), and RI (Response Index) under the P-S-R (Pressure-State-Response) classification system. Because flood indexing factors are measured at different scales and units, they are transformed into a common domain by the T-Score normalization technique. The entropy weight coefficient method is also applied to calculate the weight of flood indexing factors in order to reduce subjective judgement on the effect of weight coefficients. The three flood indexes of PI, SI, and RI are integrated for an overall value of the FRI to evaluate the flood risk of districts. To examine the practical application of the proposed FRI, the FRI results with/without the weight coefficients are compared with flooding zones of natural disaster risk areas officially announced in 2010. It is expected that the FRI ensured by full verification can make regional protection plans against flooding disasters with respect to causes and characteristics of past floods.

Hardware Implementation of Real-Time Blind Watermarking by Substituting Bitplanes of Wavelet DC Coefficients (웨이블릿 DC 계수의 비트평면 치환방법에 의한 실시간 블라인드 워터마킹 및 하드웨어 구현)

  • 서영호;김동욱
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.3C
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    • pp.398-407
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    • 2004
  • In this paper, a blind watermarking method which is suitable to the video compression using 2-D discrete wavelet transform was proposed and implemented into the hardware using VHDL(VHSIC Hardware Description Language). The goal of the proposed watermarking algorithm is the authentication about the manipulation of the watermark embedded image and the detection of the error positions. Considering the compressed video image, the proposed watermarking scheme is unrelated to the quantization and is able to concurrently embed or extract the watermark. We experimentally verified that the lowest frequency subband(LL4) is not sensitive to the change in the spatial domain, so LL4 subband was selected for the mark space. And the combination of the bitplanes which has the properties of both the minimum degradation of the image and the robustness was chosen as the embedded Point in the mark space in LL4 subband. Since we know the watermark embedded positions and the watermark is embedded by not varying the value but changing the value, the watermark can be extracted without the original image. Also, for the security when exposing the watermark embedded position, we embed the encrypted watermark by the block cipher. The proposed watermark algorithm shows the robustness against the general image manipulation and is easily transplanted into the image or video compressor with the minimal changing in the structure. The designed hardware has 4037 LABs(24%) and 85 ESBs(3%) in APEX20KC EP20K400CF672C7 FPGA of Altera and stably operates in 82MHz clock frequency.

Distribution of Soil Water Storage at a Hillslope in Sulmachun Watershed (설마천 사면의 토양층 저류량의 분포양상에 관한 연구)

  • Jang, Eun-se;Kim, Sang-Hyun;Lee, Jung-Hoon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.18 no.2
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    • pp.88-98
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    • 2016
  • The storage variation in the vadose zone at a hillslope is important to understand the hydrological process. This study explores seasonal changes of soil water storage at a hillslope scale. The study was conducted on a hillslope of Beomrunsa, located in the Seolma-cheon river basin in Paju-si, Gyeonggi-do. Using soil moisture measurements through Time Domain Reflectometry (TDR), storage, discharge, and response constant were calculated for all monitoring points on a hillslope between March and November, 2010. This study found that temporal changes in storage are resulted from the rainfall distribution patterns. Analysis of the spatial changes in storage indicated that the soil water storage tends to increase towards the downslope direction. The discharges calculated based on the soil water measurements exhibited a high correlation with observation discharge. The storage response constant was high during the autumn which demonstrates the increased contribution from upslope is responsible for the occurrence of soil water recharge during autumn.

A Fundamental Study about a Quality Certification of 3D Precision Indoor Geospatial Information - Focused on Yeongdeungpo Station - (3차원 정밀 실내공간정보 품질인증 방안에 관한 기초연구 - 영등포역을 중심으로-)

  • Lee, Ki Sung;Jeong, In Hun;Choi, Yun Soo;Kim, Sang Bong
    • Journal of Korean Society for Geospatial Information Science
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    • v.23 no.1
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    • pp.3-14
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    • 2015
  • As buildings in the downtown area, such as the complex building connected to the skyscraper and the underground facility, have become large and complex, the range of target domain of the space information service is rapidly expanding to the interior space; it is considered that this change will create various demands of service such as the indoor geospatial information base safety, the management of facility, and the interior navigation in the future, along with spread of mobile devices and development of IT technology. As for the indoor geospatial information, however, there is no certification standard of the established indoor geospatial information data quality, so preparing the certification standard is urgent. Thus, this study reviewed foreign and domestic research cases and prepared measures for quality verification of the indoor geospatial information to conduct a verification test of the Yeongdeungpo Station indoor geospatial information, established in 2014. As a result, through the verification test of the method and standard of the indoor geospatial information quality certification suggested by this research, it was identified that the uniform and higher quality data could be classified, and the types of error high frequently occurring could be investigated. These results are expected to be utilized as the basic data for establishing quality certification system for the indoor geospatial information in the future.

An Efficient Error Compensation Method for Thumbnail Extraction in H.264/AVC Bitstreams (H.264/AVC 비트스트림으로부터 썸네일 추출 시 효율적인 오차 보상 방법)

  • Yoon, Myung-Keun;Lee, Yeo-Song;Sohn, Chae-Bong;Park, Ho-Chong;Ahn, Chang-Beom;Oh, Seoung-Jun
    • Journal of Broadcast Engineering
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    • v.13 no.5
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    • pp.622-635
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    • 2008
  • Recently, high definition media services like HDTV and IPTV are growing. A fast reduced-size image extracting method is required to meet what those services require. Conventional DC image extracting methods, however, can't be applied to H.264/AVC streams since a spatial domain prediction scheme is adopted in H.264/AVC intra mode. To solve this problem, a thumbnail extraction method in H.264/AVC was proposed. However, the method has mismatch problem which was caused by round-off operation in intra prediction and mismatch between integer and floating point calculation. In this paper, we propose an error compensation method for extracting thumbnail directly in H.264/AVC bitstreams. The compensation method introduces the mismatch problem in thumbnail extraction and presents compensation values. Through the implementation and performance evaluation, proposed method compensated round-off error efficiently in D1 and HD sequences while the additional extraction time is negligible.

A Case Study of Middle School Science Teachers' Topic-Specific Pedagogical Content Knowledge on the Unit of Stars and Universe (별과 우주 단원에 대한 중학교 과학 교사의 주제-특이적 PCK 사례연구)

  • Lee, Kiyoung;Maeng, Seungho;Park, Young-Shin;Lee, Jeong-A;Oh, Hyunseok
    • Journal of The Korean Association For Science Education
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    • v.34 no.4
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    • pp.393-406
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    • 2014
  • Pedagogical Content Knowledge (PCK) is intrinsically domain/topic-specific knowledge for teachers' expertise. On the basis of this idea about PCK, we investigated in-service science teachers' topic-specific PCK on the unit of stars and universe in terms of teachers' knowledge and practices. We observed four middle school science teachers' classes for eighth graders, and took the videos of the classes. The topics of the classes covered constellation, annual parallax and the distance of stars, and the expansion of the universe. We also examined the teachers' pedagogical thinking through video stimulated recall interviews. In addition, we developed a protocol to analyze the levels of participant teachers' PCK and the characteristics revealed in their classes on each topic. Results of the study showed that the participant teachers' level of PCK varied across the topics of classes and the sub-components of PCK. We also identified teaching orientations played key roles in shaping overall characteristics of their PCK. Moreover, astronomical thinking such as spatial thinking and system thinking, was not appropriately embedded as the specific practices into the astronomical concepts in their instructions. We discussed the implications on the progressions of teachers' PCK in terms of their professional development.

Statistical Characteristics of Recent Lightning Occurred over South Korea (최근 남한지역에서 발생한 낙뢰의 통계적 특성)

  • Eom, Hyo-Sik;Suh, Myoung-Seok
    • Journal of the Korean earth science society
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    • v.30 no.2
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    • pp.210-222
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    • 2009
  • Lightning data, observed from total lightning detection system (TLDS) of KMA, for the recent five years (2002-2006) have been analyzed for temporal and spatial characteristics of frequency, intensity, duration, and flash rate. Lightning frequency varies largely with years (most frequent in 2006) and the lightning during the summer accounts for 75% of total flashes and only 0.6% of lightnings strike in cold season. In rainy season (JJAS), the ratio of positive flashes to negative ones is as low as 0.15, but it increases up to 0.98 in February. The seasonal variation of lightning duration is strongly linked with lightning occurrences, whereas flashes rates show weak seasonal variability. In a daily scale, lightning, on average, occurs more often at dawn (2 am, 5-7 am) and in the mid-afternoon (15 pm), and the lightning at dawn (around 5 am) is most intense during the day. The western inland areas md the West/South Sea show high lightning density during JJAS, whereas eastern part and the East Sea exhibit a low density of lightning. Considering the low ratio of positive flashes (0.15) for the whole analysis domain during summer period, Chungnam and Jeonbuk areas have a high ratio of flashes over 0.4. However, these should be analyzed with much caution because weak positive cloud-to-cloud discharges can be regarded as cloud-to-ground flashes. The western inland also exhibits long annual flash hours (15-24). And the W3st Sea has high flash rates as a result of large density and low flash hours. The most frequent time of lightning occurrence over most inland areas lies between mid-afternoon and early-evening, whereas mountainous and coastal areas, and the northern Kyoungki and Hwanghae provinces show the maximum lightning strikes in the morning and at dawn, respectively.

Variability and Horizontal Structure of Sea Surface Height Anomaly Estimated from Topex/poseidon Altimeter in the East (Japan) Sea (동해의 Topex/Poseidon 고도계로부터 추정된 해면고도이상치의 수평구조와 변동성)

  • Kim, Eung;Ro, Young-Jae;Kim, Chang-Shik
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.2
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    • pp.94-110
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    • 2003
  • This study utilizes the dataset of Topex/Poseidon(T/P) altimeter sea surface height (1992-2000 yr., 286 cycles)to investigate the tempore-spatial variability in the East (Japan) Sea. Optimal interpolation (Ol) technique was applied to the pre-processed T/P dataset (level 2) to produce sea surface height anomaly (SSHA) map on regular grids. Spectral analyses of the timeseries of the SSHA at chosen stations and empirical orthogonal function (EOF) analysis of the SSHA in the entire East Sea were made. Distribution of the SSHA can be divided by the southern and northern regions sharply by the polar front situated in the middle of the East Sea. The southern region under the direct influence of the Tsushima Current exhibits higher amplitude of the SSHA fluctuation, while the northern region does relatively smaller one. The spatio-temporal variability of the SSHA in the East Sea can be characterized by the five modes of the EOFs accounting for more than 85% of the total variance. The first mode dominates the SSHA variation in the entire domain with strong seasonal and inter-annual periods accounting for the 72.3% of the total variance. The other modes (up to 5th account for 14%) are responsible for the SSHA variation associated with the local current system, meandering of the polar frontal axis, and mesoscale eddies. Spectral peaks with significant confluence level show semi-annual, annual and interannual (2, 3-4 years) periods.